191 research outputs found

    Compilation and validation of SAR and optical data products for a complete and global map of inland/ocean water tailored to the climate modeling community

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    Accurate maps of surface water extent are of paramount importance for water management, satellite data processing and climate modeling. Several maps of water bodies based on remote sensing data have been released during the last decade. Nonetheless, none has a truly (90°N/90°S) global coverage while being thoroughly validated. This paper describes a global, spatially-complete (void-free) and accurate mask of inland/ocean water for the 2000–2012 period, built in the framework of the European Space Agency (ESA) Climate Change Initiative (CCI). This map results from the synergistic combination of multiple individual SAR and optical water body and auxiliary datasets. A key aspect of this work is the original and rigorous stratified random sampling designed for the quality assessment of binary classifications where one class is marginally distributed. Input and consolidated products were assessed qualitatively and quantitatively against a reference validation database of 2110 samples spread throughout the globe. Using all samples, overall accuracy was always very high among all products, between 98% and 100%. The CCI global map of open water bodies provided the best water class representation (F-score of 89%) compared to its constitutive inputs. When focusing on the challenging areas for water bodies’ mapping, such as shorelines, lakes and river banks, all products yielded substantially lower accuracy figures with overall accuracies ranging between 74% and 89%. The inland water area of the CCI global map of open water bodies was estimated to be 3.17 million km2 ± 0.24 million km2. The dataset is freely available through the ESA CCI Land Cover viewer

    Canopy-scale biophysical controls of transpiration and evaporation in the Amazon Basin.

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    Canopy and aerodynamic conductances (gC and gA) are two of the key land surface biophysical variables that control the land surface response of land surface schemes in climate models. Their representation is crucial for predicting transpiration (λET) and evaporation (λEE) flux components of the terrestrial latent heat flux (λE), which has important implications for global climate change and water resource management. By physical integration of radiometric surface temperature (TR) into an integrated framework of the Penman?Monteith and Shuttleworth?Wallace models, we present a novel approach to directly quantify the canopy-scale biophysical controls on λET and λEE over multiple plant functional types (PFTs) in the Amazon Basin. Combining data from six LBA (Large-scale Biosphere-Atmosphere Experiment in Amazonia) eddy covariance tower sites and a TR-driven physically based modeling approach, we identified the canopy-scale feedback-response mechanism between gC, λET, and atmospheric vapor pressure deficit (DA), without using any leaf-scale empirical parameterizations for the modeling. The TR-based model shows minor biophysical control on λET during the wet (rainy) seasons where λET becomes predominantly radiation driven and net radiation (RN) determines 75 to 80 % of the variances of λET. However, biophysical control on λET is dramatically increased during the dry seasons, and particularly the 2005 drought year, explaining 50 to 65 % of the variances of λET, and indicates λET to be substantially soil moisture driven during the rainfall deficit phase. Despite substantial differences in gA between forests and pastures, very similar canopy?atmosphere "coupling" was found in these two biomes due to soil moisture-induced decrease in gC in the pasture. This revealed the pragmatic aspect of the TR-driven model behavior that exhibits a high sensitivity of gC to per unit change in wetness as opposed to gA that is marginally sensitive to surface wetness variability. Our results reveal the occurrence of a significant hysteresis between λET and gC during the dry season for the pasture sites, which is attributed to relatively low soil water availability as compared to the rainforests, likely due to differences in rooting depth between the two systems. Evaporation was significantly influenced by gA for all the PFTs and across all wetness conditions. Our analytical framework logically captures the responses of gC and gA to changes in atmospheric radiation, DA, and surface radiometric temperature, and thus appears to be promising for the improvement of existing land?surface?atmosphere exchange parameterizations across a range of spatial scales

    Canopy-scale biophysical controls on transpiration and evaporation in the Amazon Basin

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    Canopy and aerodynamic conductances (gC and gA) are two of the key land surface biophysical variables that control the land surface response of land surface schemes in climate models. Their representation is crucial for predicting transpiration (?ET) and evaporation (?EE) flux components of the terrestrial latent heat flux (?E), which has important implications for global climate change and water resource management. By physical integration of radiometric surface temperature (TR) into an integrated framework of the Penman?Monteith and Shuttleworth?Wallace models, we present a novel approach to directly quantify the canopy-scale biophysical controls on ?ET and ?EE over multiple plant functional types (PFTs) in the Amazon Basin. Combining data from six LBA (Large-scale Biosphere-Atmosphere Experiment in Amazonia) eddy covariance tower sites and a TR-driven physically based modeling approach, we identified the canopy-scale feedback-response mechanism between gC, ?ET, and atmospheric vapor pressure deficit (DA), without using any leaf-scale empirical parameterizations for the modeling. The TR-based model shows minor biophysical control on ?ET during the wet (rainy) seasons where ?ET becomes predominantly radiation driven and net radiation (RN) determines 75 to 80?% of the variances of ?ET. However, biophysical control on ?ET is dramatically increased during the dry seasons, and particularly the 2005 drought year, explaining 50 to 65?% of the variances of ?ET, and indicates ?ET to be substantially soil moisture driven during the rainfall deficit phase. Despite substantial differences in gA between forests and pastures, very similar canopy?atmosphere "coupling" was found in these two biomes due to soil moisture-induced decrease in gC in the pasture. This revealed the pragmatic aspect of the TR-driven model behavior that exhibits a high sensitivity of gC to per unit change in wetness as opposed to gA that is marginally sensitive to surface wetness variability. Our results reveal the occurrence of a significant hysteresis between ?ET and gC during the dry season for the pasture sites, which is attributed to relatively low soil water availability as compared to the rainforests, likely due to differences in rooting depth between the two systems. Evaporation was significantly influenced by gA for all the PFTs and across all wetness conditions. Our analytical framework logically captures the responses of gC and gA to changes in atmospheric radiation, DA, and surface radiometric temperature, and thus appears to be promising for the improvement of existing land?surface?atmosphere exchange parameterizations across a range of spatial scales

    The Effect of Oxidant and the Non-Oxidant Alteration of Cellular Thiol Concentration on the Formation of Protein Mixed-Disulfides in HEK 293 Cells

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    Cellular molecules possess various mechanisms in responding to oxidant stress. In terms of protein responses, protein S-glutathionylation is a unique post-translational modification of protein reactive cysteines forming disulfides with glutathione molecules. This modification has been proposed to play roles in antioxidant, regulatory and signaling in cells under oxidant stress. Recently, the increased level of protein S-glutathionylation has been linked with the development of diseases. In this report, specific S-glutathionylated proteins were demonstrated in human embryonic kidney 293 cells treated with two different oxidative reagents: diamide and hydrogen peroxide. Diamide is a chemical oxidizing agent whereas hydrogen peroxide is a physiological oxidant. Under the experimental conditions, these two oxidants decreased glutathione concentration without toxicity. S-glutathionylated proteins were detected by immunoblotting and glutathione concentrations were determined by high performance liquid chromatography. We further show the effect of alteration of the cellular thiol pool on the amount of protein S-glutathionylation in oxidant-treated cells. Cellular thiol concentrations were altered either by a specific way using buthionine sulfoximine, a specific inhibitor of glutathione biosynthesis or by a non-specific way, incubating cells in cystine-methionine deficient media. Cells only treated with either buthionine sulfoximine or cystine-methionine deficient media did not induce protein S-glutathionylation, even though both conditions decreased 65% of cellular glutathione. Moreover, the amount of protein S-glutathionylation under both conditions in the presence of oxidants was not altered when compared to the amount observed in regular media with oxidants present. Protein S-glutathionylation is a dynamic reaction which depends on the rate of adding and removing glutathione. Phenylarsine oxide, which specifically forms a covalent adduct with vicinal thiols, was used to determine the possible role of vicinal thiols in the amount of glutathionylation. Our data shows phenylarsine oxide did not change glutathione concentrations, but it did enhance the amount of glutathionylation in oxidant-treated cells

    Quantifying local rainfall dynamics and uncertain boundary conditions into a nested regional-local flood modeling system

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    [Abstract:] Inflow discharge and outflow stage estimates for hydraulic flood models are generally derived from river gauge data. Uncertainties in the measured inflow data and the neglect of rainfall-runoff contributions to the modeled domain downstream of the gauging locations can have a significant impact on these estimated “whole reach” inflows and consequently on flood predictions. In this study, a method to incorporate rating curve uncertainty and local rainfall-runoff dynamics into the predictions of a reach-scale flood model is proposed. The methodology is applied to the July 2007 floods of the River Severn in UK. Discharge uncertainty bounds are generated applying a nonparametric local weighted regression approach to stage-discharge measurements for two gauging stations. Measured rainfall downstream from these locations is used as input to a series of subcatchment regional hydrological model to quantify additional local inflows along the main channel. A regional simplified-physics hydraulic model is then applied to combine these contributions and generate an ensemble of discharge and water elevation time series at the boundaries of a local-scale high complexity hydraulic model. Finally, the effect of these rainfall dynamics and uncertain boundary conditions are evaluated on the local-scale model. Accurate prediction of the flood peak was obtained with the proposed method, which was only possible by resolving the additional complexity of the extreme rainfall contributions over the modeled area. The findings highlight the importance of estimating boundary condition uncertainty and local rainfall contributions for accurate prediction of river flows and inundation at regional scales.María Bermúdez gratefully acknowledges financial support from the Spanish Regional Government of Galicia (postdoctoral grant reference ED481B 2014/156-0). Gemma Coxon was supported by NERC MaRIUS: Managing the Risks, Impacts and Uncertainties of droughts and water Scarcity, grant NE/L010399/1. Jim Freer and Paul Bates by NERC Susceptibility of catchments to INTense RAinfall and flooding, grant NE/K00882X/1. A free version of the model LISFLOOD-FP is available for download at www.bristol.ac.uk/geography/research/hydrology/models/lisflood/. A free version of the model Iber is available for download at www.iberaula.es. The river cross-section data, the LiDAR digital elevation model and the gauging station rainfall, stage, flow and rating curve data of the presented case study are freely available from the Environment Agency ([email protected])Xunta de Galicia; ED481B 2014/156-0Inglaterra. University of Bristol; NE/L010399/1Inglaterra. University of Bristol; NE/K00882X/

    Network Inference Algorithms Elucidate Nrf2 Regulation of Mouse Lung Oxidative Stress

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    A variety of cardiovascular, neurological, and neoplastic conditions have been associated with oxidative stress, i.e., conditions under which levels of reactive oxygen species (ROS) are elevated over significant periods. Nuclear factor erythroid 2-related factor (Nrf2) regulates the transcription of several gene products involved in the protective response to oxidative stress. The transcriptional regulatory and signaling relationships linking gene products involved in the response to oxidative stress are, currently, only partially resolved. Microarray data constitute RNA abundance measures representing gene expression patterns. In some cases, these patterns can identify the molecular interactions of gene products. They can be, in effect, proxies for protein–protein and protein–DNA interactions. Traditional techniques used for clustering coregulated genes on high-throughput gene arrays are rarely capable of distinguishing between direct transcriptional regulatory interactions and indirect ones. In this study, newly developed information-theoretic algorithms that employ the concept of mutual information were used: the Algorithm for the Reconstruction of Accurate Cellular Networks (ARACNE), and Context Likelihood of Relatedness (CLR). These algorithms captured dependencies in the gene expression profiles of the mouse lung, allowing the regulatory effect of Nrf2 in response to oxidative stress to be determined more precisely. In addition, a characterization of promoter sequences of Nrf2 regulatory targets was conducted using a Support Vector Machine classification algorithm to corroborate ARACNE and CLR predictions. Inferred networks were analyzed, compared, and integrated using the Collective Analysis of Biological Interaction Networks (CABIN) plug-in of Cytoscape. Using the two network inference algorithms and one machine learning algorithm, a number of both previously known and novel targets of Nrf2 transcriptional activation were identified. Genes predicted as novel Nrf2 targets include Atf1, Srxn1, Prnp, Sod2, Als2, Nfkbib, and Ppp1r15b. Furthermore, microarray and quantitative RT-PCR experiments following cigarette-smoke-induced oxidative stress in Nrf2+/+ and Nrf2−/− mouse lung affirmed many of the predictions made. Several new potential feed-forward regulatory loops involving Nrf2, Nqo1, Srxn1, Prdx1, Als2, Atf1, Sod1, and Park7 were predicted. This work shows the promise of network inference algorithms operating on high-throughput gene expression data in identifying transcriptional regulatory and other signaling relationships implicated in mammalian disease
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