Reacciones al conflicto y ansiedad en el logro de aprendizaje de los cadetes de Comunicaciones de la Escuela Militar de Chorrillos Coronel Francisco Bolognesi, 2018

El estudio tiene como objetivo general determinar la relación entre las reacciones al conflicto y la ansiedad en el logro de aprendizaje de los cadetes de Comunicaciones de la Escuela Militar de Chorrillos Coronel Francisco Bolognesi, 2018, para el caso, utilizó un enfoque cuantitativo, de tipo básico, con un diseño no experimental, transeccional correlacional causal, en una población - muestra, denominado muestreo censal, conformado por 120 cadetes de Comunicaciones. La técnica de recolección de datos empleado para las variables reacciones al conflicto y ansiedad fue la encuesta, que utilizó como instrumentos, dos cuestionarios, mientras que para la variable logro de aprendizaje se utilizó la técnica de evaluación educativa cuyo instrumento fue el registro de notas de aprendizaje. La validez de contenido de los instrumentos se hizo a través de juicio de expertos con resultados de aplicable, y el nivel de confiabilidad de los instrumentos fue excelente, realizados mediante la prueba Alfa de Cronbach, cuyo valor fue 0,858 para la variable independiente (X1): Reacciones al conflicto (30 ítems) y 0,914 para la variable independiente (X2): Ansiedad (30 ítems) y para el caso de la variable dependiente (Y1): Logro de aprendizaje (al tratarse de actas de notas emitidas por la EMCH no se aplica pruebas de confiabilidad). Los resultados de la investigación permitieron concluir que: Existe relación significativa entre las reacciones al conflicto y la ansiedad en el logro de aprendizaje de los cadetes de Comunicaciones de la Escuela Militar de Chorrillos Coronel Francisco Bolognesi, 2018, con un p < 0,05 y la correlación r de Pearson = 0,905 (X1*Y1) correlación positiva muy alta y r de Pearson = -0,805 (X2*Y1) correlación negativa muy alta.The study's general objective is to determine the relationship between reactions to conflict and anxiety in the learning achievement of the Communications Cadets of the Military School of Chorrillos Coronel Francisco Bolognesi, 2018, for the case, it used a quantitative approach, of the type Basic, with a non-experimental, causal correlational cross-sectional design, in a sample population, called census sampling, made up of 120 Communications cadets. The data collection technique used for the variables reactions to conflict and anxiety was the survey, which used two questionnaires as instruments, while the educational evaluation technique was used for the variable achievement of learning, the instrument of which was the recording of learning. The content validity of the instruments was made through expert judgment with applicable results, and the level of reliability of the instruments was excellent, performed using the Cronbach's Alpha test, whose value was 0.858 for the independent variable (X1). : Reactions to the conflict (30 items) and 0.914 for the independent variable (X2): Anxiety (30 items) and in the case of the dependent variable (Y1): Learning achievement (it was not necessary to apply reliability, as they are records of notes issued by the EMCH). The results of the research allowed us to conclude that: There is a significant relationship between reactions to conflict and anxiety in the learning achievement of the Communications Cadets of the Military School of Chorrillos Coronel Francisco Bolognesi, 2018, with a p <0.05 and the correlation Pearson's r = 0.905 (X1 * Y1) very high positive correlation and Pearson's r = -0.805 (X2 * Y1) very high negative correlation

Symbiotic control of the olive fruit fly, Bactrocera oleae

The olive fruit fly Bactrocera oleae (Rossi) (OLF) is a major pest, which causes severe yield losses and quality decay of olive oil. The widespread use of chemical insecticides to control this pest is a major concern for the resulting environmental impact and food safety issues. Therefore, the development of sustainable control strategies is highly desirable. The primary endosymbiotic bacterium of the OLF, “Candidatus Erwinia dacicola”, is essential for successful larval development in unripe olive fruits. Then, targeting this endosymbiont with antimicrobial compounds may exert a control action against OLF. Here we evaluate the impact on OLF endosymbiont of Copper Oxychloride (CO) and the fungal metabolites Viridiol and Harzianic Acid (HA) produced by two biocontrol strains of Trichoderma spp. Laboratory bioassays were carried out on OLF wild populations to assess the effect of the oral administration of these compounds on mortality and fecundity of adult flies, and on larval development of their progeny in unripe olive fruits. Treated females were processed by qPCR to measure the endosymbiont load in the oesophageal bulb and in the midgut. Exposure to Viridiol and HA had a strong negative impact on endosymbiont load and OLF larval survival, while CO negatively affected both adults and larval stages, showing a combined toxic action and an anti-symbiotic effect, which was dose-dependent. These results provide new insights on the symbiotic control of the OLF and pave the way for developing new strategies based on the use of natural compounds with antimicrobial activity

Role of redox environment on the oligomerization of higher molecular weight adiponectin

<p>Abstract</p> <p>Background</p> <p>Adiponectin is an adipocyte-secreted hormone with insulin-sensitizing and anti-inflammatory actions. The assembly of trimeric, hexameric, and higher molecular weight (HMW) species of adiponectin is a topic of significant interest because physiological actions of adiponectin are oligomer-specific. In addition, adiponectin assembly is an example of oxidative oligomerization of multi-subunit protein complexes in endoplasmic reticulum (ER).</p> <p>Results</p> <p>We previously reported that trimers assemble into HMW adiponectin <it>via </it>intermediates stabilized by disulfide bonds, and complete oxidation of available cysteines locks adiponectin in hexameric conformation. In this study, we examined the effects of redox environment on the rate of oligomer formation and the distribution of oligomers. Reassembly of adiponectin under oxidizing conditions accelerated disulfide bonding but favored formation of hexamers over the HMW species. Increased ratios of HMW to hexameric adiponectin could be achieved rapidly under oxidizing conditions by promoting disulfide rearrangement.</p> <p>Conclusions</p> <p>Based upon these observations, we propose oxidative assembly of multi-subunit adiponectin complexes in a defined and stable redox environment is favored under oxidizing conditions coupled with high rates of disulfide rearrangement.</p

Acetylsalicylic acid prevents intermittent hypoxia-induced vascular remodeling in a murine model of sleep apnea

Study objectives: Chronic intermittent hypoxia (CIH), a hallmark feature of obstructive sleep apnea (OSA), induces accelerated atherogenesis as well as aorta vascular remodeling. Although the cyclooxygenase (COX) pathway has been proposed to contribute to the cardiovascular consequences of OSA, the potential benefits of a widely employed COX-inhibitor such (acetylsalicylic acid, ASA) on CIH-induced vascular pathology are unknown. Therefore, we hypothesized that a common non-selective COX inhibitor such as ASA would attenuate the aortic remodeling induced by CIH in mice. Methods: 40 wild-type C57/BL6malemice were randomly allocated to CIH or normoxic exposures (N) and treated with daily doses of ASA or placebo for 6 weeks. At the end of the experiments, intima-media thickness (IMT), elastin disorganization (ED), elastin fragmentation (EF), length between fragmented fiber endpoints (LFF), aortic wall collagen abundance (AC) and mucoid deposition (MD) were assessed. Results: Compared to N, CIH promoted significant increases in IMT (52.58 ± 2.82μm vs. 46.07 ± 4.18μm, p < 0.003), ED (25.29 ± 14.60% vs. 4.74 ± 5.37%, p < 0.001), EF (5.80 ± 2.04 vs. 3.06 ± 0.58, p < 0.001), LFF (0.65 ± 0.34% vs. 0.14 ± 0.09%, p < 0.001), AC (3.43 ± 1.52% vs. 1.67 ± 0.67%, p < 0.001) and MD (3.40 ± 2.73 μm2 vs. 1.09 ± 0.72 μm2, p < 0.006). ASA treatment mitigated the CIH-induced alterations in IMT: 44.07 ± 2.73μm; ED: 10.57 ± 12.89%; EF: 4.63 ± 0.88; LFF: 0.25 ± 0.17% and AC: 0.90 ± 0.13% (p<0.05 for all comparisons). Conclusions: ASA prevents the CIH-induced aortic vascular remodeling, and should therefore be prospectively evaluated as adjuvant treatment in patients with OSA.This work was supported by the Spanish Respiratory Society (SEPAR), SOCAP, the Associació Lleidatana de Respiratori (ALLER), and the Spanish Fondo de Investigaciones Sanitarias (PI14/00486 and PI14-00004), Instituto de Salud Carlos III (ISCIII), European Regional Development Fund (ERDF) “Una manera de hacer Europa”. DG is supported by National Institutes of Health grant HL130984

Collecting wild potato species (Solanum sect. Petota) in Peru to enhance genetic representation and fill gaps in ex situ collections

Crop wild relatives (CWRs) are important sources of novel genes, due to their high variability of response to biotic and abiotic stresses, which can be invaluable for crop genetic improvement programs. Recent studies have shown that CWRs are threatened by several factors, including changes in land-use and climate change. A large proportion of CWRs are underrepresented in genebanks, making it necessary to take action to ensure their long-term ex situ conservation. With this aim, 18 targeted collecting trips were conducted during 2017/2018 in the center of origin of potato (Solanum tuberosum L.), targeting 17 diverse ecological regions of Peru. This was the first comprehensive wild potato collection in Peru in at least 20 years and encompassed most of the unique habitats of potato CWRs in the country. A total of 322 wild potato accessions were collected as seed, tubers, and whole plants for ex situ storage and conservation. They belonged to 36 wild potato species including one accession of S. ayacuchense that was not conserved previously in any genebank. Most accessions required regeneration in the greenhouse prior to long-term conservation as seed. The collected accessions help reduce genetic gaps in ex situ conserved germplasm and will allow further research questions on potato genetic improvement and conservation strategies to be addressed. These potato CWRs are available by request for research, training, and breeding purposes under the terms of the International Treaty for Plant Genetic Resources for Food and Agriculture (ITPGRFA) from the Instituto Nacional de Innovacion Agraria (INIA) and the International Potato Center (CIP) in Lima-Peru

Cardiomyocyte hypertrophy induced by Endonuclease G deficiency requires reactive oxygen radicals accumulation and is inhibitable by the micropeptide humanin

The endonuclease G gene (Endog), which codes for a mitochondrial nuclease, was identified as a determinant of cardiac hypertrophy. How ENDOG controls cardiomyocyte growth is still unknown. Thus, we aimed at finding the link between ENDOG activity and cardiomyocyte growth. Endog deficiency induced reactive oxygen species (ROS) accumulation and abnormal growth in neonatal rodent cardiomyocytes, altering the AKT-GSK3 beta and Class-II histone deacethylases (HDAC) signal transduction pathways. These effects were blocked by ROS scavengers. Lack of ENDOG reduced mitochondrial DNA (mtDNA) replication independently of ROS accumulation. Because mtDNA encodes several subunits of the mitochondrial electron transport chain, whose activity is an important source of cellular ROS, we investigated whether Endog deficiency compromised the expression and activity of the respiratory chain complexes but found no changes in these parameters nor in ATP content. MtDNA also codes for humanin, a micropeptide with possible metabolic functions. Nanomolar concentrations of synthetic humanin restored normal ROS levels and cell size in Endog-deficient cardiomyocytes. These results support the involvement of redox signaling in the control of cardiomyocyte growth by ENDOG and suggest a pathway relating mtDNA content to the regulation of cell growth probably involving humanin, which prevents reactive oxygen radicals accumulation and hypertrophy induced by Endog deficiency.This work was supported by Grant SAF2013-44942R from the Ministerio de Economia y Competitividad (MINECO) to DS, Grant 20153810 from Fundacio La Marato de TV3 to DS, Program ``Redes Tematicas de Investigacion Cooperativa en Salud´´ (RETICS) Grants RD12/0042/0035, RD12/0042/0056 and RD12/0042/0021, Red de Investigacion Cardiovascular (RIC) from the Institute de Salud Carlos-III (ISCIII) to DS, DG-D and JV, Grant 2009SGR-346 from the Agencia de Gestic d'Ajuts Universitaris i de Recerca (AGAUR) from the Government of Catalonia to DS. AB is supported by Fundacio La Marato de TV3 and GB is supported by a predoctoral contract from the Universitat de Lleida.S

Agencia Pizca

El presente trabajo tiene como objetivo conocer y profundizar en el público objetivo de Matchcota y realizar una campaña con los hallazgos de esta investigación. Iniciamos la investigación con un pedido de la marca Matchcota, una plataforma virtual de adopción que busca aumentar la cantidad de adopciones mensuales. Para ello necesitaban ampliar su alcance mediante una campaña atractiva, potente, creativa y sobre todo que se ajuste al presupuesto que la marca proporciona. En la investigación se utilizaron herramientas cuantitativas, como encuestas y Facebook Audience Insights, y herramientas cualitativas, como focus groups, entrevistas y netnografía. De esta manera la investigación empezó con un análisis de la información que proporcionaba Facebook sobre el público y con una encuesta para aproximarnos a la información demográfica del público. Posteriormente realizamos dos focus groups para profundizar en la relación del público con sus mascotas y su opinión sobre la adopción. Finalmente, realizamos entrevistas para profundizar aún más en nuestro público. Posteriormente utilizamos herramientas para ordenar la información y los hallazgos encontrados, lo cual nos serviría para establecer una estrategia creativa y de medios. Entre los hallazgos más importantes encontramos que el público principal son mujeres, también encontramos que consideran a la mascota como un miembro más de la familia y que una mascota les permite descubrirse a sí mismos, se generan emociones y comportamientos nuevo

Tyrosine 331 and phenylalanine 334 in Clostridium perfringens α-toxin are essential for cytotoxic activity

AbstractDifferences in the biological properties of the Clostridium perfringens phospholipase C (α-toxin) and the C. bifermentans phospholipase C (Cbp) have been attributed to differences in their carboxy-terminal domains. Three residues in the carboxy-terminal domain of α-toxin, which have been proposed to play a role in membrane recognition (D269, Y331 and F334), are not conserved in Cbp (Y, L and I respectively). We have characterised D269Y, Y331L and F334I variant forms of α-toxin. Variant D269Y had reduced phospholipase C activity towards aggregated egg yolk phospholipid but increased haemolytic and cytotoxic activity. Variants Y331L and F334I showed a reduction in phospholipase C, haemolytic and cytotoxic activities indicating that these substitutions contribute to the reduced haemolytic and cytotoxic activity of Cbp

The Muon Anomalous Magnetic Moment and the Standard Model

The muon anomalous magnetic moment measurement, when compared with theory, can be used to test many extensions to the standard model. The most recent measurement made by the Brookhaven E821 Collaboration reduces the uncertainty on the world average of a_mu to 0.7 ppm, comparable in precision to theory. This paper describes the experiment and the current theoretical efforts to establish a correct standard model reference value for the muon anomaly.Comment: Plenary Talk; PANIC'02 XVI Particles and Nuclear International Conference, Osaka, Japan; Sept. 30 - Oct. 4, 2002; Report describes the published 0.7 ppm result and updates the theory statu

A network perspective on the ecology of gut microbiota and progression of type 2 diabetes: Linkages to keystone taxa in a Mexican cohort

IntroductionThe human gut microbiota (GM) is a dynamic system which ecological interactions among the community members affect the host metabolism. Understanding the principles that rule the bidirectional communication between GM and its host, is one of the most valuable enterprise for uncovering how bacterial ecology influences the clinical variables in the host.MethodsHere, we used SparCC to infer association networks in 16S rRNA gene amplicon data from the GM of a cohort of Mexican patients with type 2 diabetes (T2D) in different stages: NG (normoglycemic), IFG (impaired fasting glucose), IGT (impaired glucose tolerance), IFG + IGT (impaired fasting glucose plus impaired glucose tolerance), T2D and T2D treated (T2D with a 5-year ongoing treatment).ResultsBy exploring the network topology from the different stages of T2D, we observed that, as the disease progress, the networks lose the association between bacteria. It suggests that the microbial community becomes highly sensitive to perturbations in individuals with T2D. With the purpose to identify those genera that guide this transition, we computationally found keystone taxa (driver nodes) and core genera for a Mexican T2D cohort. Altogether, we suggest a set of genera driving the progress of the T2D in a Mexican cohort, among them Ruminococcaceae NK4A214 group, Ruminococcaceae UCG-010, Ruminococcaceae UCG-002, Ruminococcaceae UCG-005, Alistipes, Anaerostipes, and Terrisporobacter.DiscussionBased on a network approach, this study suggests a set of genera that can serve as a potential biomarker to distinguish the distinct degree of advances in T2D for a Mexican cohort of patients. Beyond limiting our conclusion to one population, we present a computational pipeline to link ecological networks and clinical stages in T2D, and desirable aim to advance in the field of precision medicine