Correspondence: Between DWC Task Forces and Committees. 1990-1999

Primarily incoming and outgoing letters regarding individual committee activities, nominations for awards and elections, and membership issues

Decreased microbial co-occurrence network stability and SCFA receptor level correlates with obesity in African-origin women.

We compared the gut microbial populations in 100 women, from rural Ghana and urban US [50% lean (BMI < 25 kg/m2) and 50% obese (BMI ≥ 30 kg/m2)] to examine the ecological co-occurrence network topology of the gut microbiota as well as the relationship of short chain fatty acids (SCFAs) with obesity. Ghanaians consumed significantly more dietary fiber, had greater microbial alpha-diversity, different beta-diversity, and had a greater concentration of total fecal SCFAs (p-value < 0.002). Lean Ghanaians had significantly greater network density, connectivity and stability than either obese Ghanaians, or lean and obese US participants (false discovery rate (FDR) corrected p-value ≤ 0.01). Bacteroides uniformis was significantly more abundant in lean women, irrespective of country (FDR corrected p < 0.001), while lean Ghanaians had a significantly greater proportion of Ruminococcus callidus, Prevotella copri, and Escherichia coli, and smaller proportions of Lachnospiraceae, Bacteroides and Parabacteroides. Lean Ghanaians had a significantly greater abundance of predicted microbial genes that catalyzed the production of butyric acid via the fermentation of pyruvate or branched amino-acids, while obese Ghanaians and US women (irrespective of BMI) had a significantly greater abundance of predicted microbial genes that encoded for enzymes associated with the fermentation of amino-acids such as alanine, aspartate, lysine and glutamate. Similar to lean Ghanaian women, mice humanized with stool from the lean Ghanaian participant had a significantly lower abundance of family Lachnospiraceae and genus Bacteroides and Parabacteroides, and were resistant to obesity following 6-weeks of high fat feeding (p-value < 0.01). Obesity-resistant mice also showed increased intestinal transcriptional expression of the free fatty acid (Ffa) receptor Ffa2, in spite of similar fecal SCFAs concentrations. We demonstrate that the association between obesity resistance and increased predicted ecological connectivity and stability of the lean Ghanaian microbiota, as well as increased local SCFA receptor level, provides evidence of the importance of robust gut ecologic network in obesity

Sexual violence in older adults : a Belgian prevalence study

Background: Sexual violence (SV) is an important public health problem which may cause long-lasting health problems. SV in older adults remains neglected in research, policies and practices. Valid SV prevalence estimates and associated risk factors in older adults are currently unavailable. Objective: To measure lifetime and past 12-months sexual victimisation in older adults living in Belgium, its correlates, assailant characteristics and the way that victims framed their SV experiences. Design: Cross-sectional general population study. Setting: Community-dwelling, assisted living and nursing homes. Participants: 513 people of 70 years and older living in Belgium. Methods: SV was measured using behaviourally specific questions based on a broad definition of SV. Participants were selected via a cluster random probability sampling with a random route finding approach. Information on sexual victimisation, correlates, assailant characteristics and framing was collected via structured face-to-face interviews. Results: Lifetime SV prevalence was 44% (55% F, 29% M). Past 12-months prevalence was 8% (9% F, 8% M). Female sex and a higher number of sexual partners were associated with lifetime SV (p <.05), non-heterosexual sexual orientation with past 12-months SV (p <.05). Correlates generally linked to elder abuse and neglect were not linked with SV. ‘Someone unknown’ was identified as most common assailant. Conclusions: SV appears to be common in older adults in Belgium. Both correlates and assailant characteristics seem to differ from previous studies on elder abuse and neglect. Recognising older adults as a risk group for sexual victimisation in research, policies and practices is of the utmost importance

Prospectus, December 15, 1977

LETTERS TO THE EDITORS: SLACK ANNOUNCES FOR STATE REP., DO THE STUDENTS KNOW?; Finals schedule revised; Star Wars cashes in on Sci-Fi movie craze; Nutcracker danced by National Ballet; Pre-school kids know why they have Christmas; Close encounters of a red and green kind; What else is new: Drivers bad?; Accidents up with sales: Experts: beware of chain saw dangers; ...and speaking of chain saws: America finds new fuel...wood; U. of I. art at krannert; A Prairie Winter: \u27Whose woods these are...\u27; Carl Sandburg: 100 new poems published; Guest artist\u27s choreography entertaining, full of surprises; Bach backers have cause to rejoice; Dirty heating equipment can make your gas bill skyrocket; Physical Science Division entices hi-schoolers with freebees, rap sessions, counseling; Santa\u27s psyche probed by his errant elves; Who was that Santa I saw you with last night?; Settlers thought Illinois land was worthless; New cases for C-U city police: parent abuse; Goodbye, yellow notched card: Computerized circulation at UI Library; Humane Society still has puppies, kittens for gifts; Life doesn\u27t end at 40, either: Seniors Center fights \u27mope at home syndrome\u27; Thimbles and Threads: home-made surprises; Book review: Elvis-What Happened?; New studies of cholesterol: Eggs not villainous after all??; Warfield, Madrigals in UI holiday broadcast; Poet\u27s Corner: The Flute; Allerton Park: As the leaves fall, the people leave; Some assistants please: The Legends of Lawrenceville; Meanwhile, back in the Cobra\u27s camp...; Greatest names of golf fame ready themselves for tourney; Former Parkland student now high school assistant coach; Home run hero hits twin towns; Classifieds; Can Illinois challenge Northwestern?: Mad challenge for NCAA supremacy; Snow stalls area basketball but not for long in Illinois; Weather, Freddy bury Bob in snow; Cobras to play tough teams during break; \u27He doesn\u27t seem big to me\u27: Student has special Cowboy Additional material: Edition 3 of the Parkland literary magazinehttps://spark.parkland.edu/prospectus_1977/1000/thumbnail.jp

Store-Operated Ca2+ Entry (SOCE) and Purinergic Receptor-Mediated Ca2+ Homeostasis in Murine bv2 Microglia Cells: Early Cellular Responses to ATP-Mediated Microglia Activation

Microglia activation is a neuroinflammatory response to parenchymal damage with release of intracellular metabolites, e.g., purines, and signaling molecules from damaged cells. Extracellular purines can elicit Ca(2+)-mediated microglia activation involving P2X/P2Y receptors with metabotropic (P2Y) and ionotropic (P2X) cell signaling in target cells. Such microglia activation results in increased phagocytic activity, activation of their inflammasome and release of cytokines to sustain neuroinflammatory (so-called M1/M2 polarization). ATP-induced activation of ionotropic P2X4 and P2X7 receptors differentially induces receptor-operated Ca(2+) entry (ROCE). Although store-operated Ca(2+) entry (SOCE) was identified to modulate ROCE in primary microglia, its existence and role in one of the most common murine microglia cell line, BV2, is unknown. To dissect SOCE from ROCE in BV2 cells, we applied high-resolution multiphoton Ca(2+) imaging. After depleting internal Ca(2+) stores, SOCE was clearly detectable. High ATP concentrations (1 mM) elicited sustained increases in intracellular [Ca(2+)]i whereas lower concentrations (≤100 μM) also induced Ca(2+) oscillations. These differential responses were assigned to P2X7 and P2X4 activation, respectively. Pharmacologically inhibiting P2Y and P2X responses did not affect SOCE, and in fact, P2Y-responses were barely detectable in BV2 cells. STIM1S content was significantly upregulated by 1 mM ATP. As P2X-mediated Ca(2+) oscillations were rare events in single cells, we implemented a high-content screening approach that allows to record Ca(2+) signal patterns from a large number of individual cells at lower optical resolution. Using automated classifier analysis, several drugs (minocycline, U73122, U73343, wortmannin, LY294002, AZ10606120) were tested on their profile to act on Ca(2+) oscillations (P2X4) and sustained [Ca(2+)]i increases. We demonstrate specific drug effects on purinergic Ca(2+) pathways and provide new pharmacological insights into Ca(2+) oscillations in BV2 cells. For example, minocycline inhibits both P2X7- and P2X4-mediated Ca(2+)-responses, and this may explain its anti-inflammatory action in neuroinflammatory disease. As a technical result, our novel automated bio-screening approach provides a biomedical engineering platform to allow high-content drug library screens to study neuro-inflammation in vitro