Population genetic structure of a sandstone specialist and a generalist heath species at two levels of sandstone patchiness across the Strait of Gibraltar

Many habitat specialist species are originally composed of small, discontinuous populations because their habitats are naturally fragmented or patchy. They may have suffered the long-term effects of natural patchiness. Mediterranean heathlands, a representative habitat in the Strait of Gibraltar region, are associated with nutrient-poor, acidic sandstone soils. Sandstone soil patches in the African side of the Strait (Tangier) are, in general, smaller and more scattered than in the European side (Algeciras). In this study, we analyze the effect of this sandstone patchiness on the population genetic diversity and structure of two Erica species from these Mediterranean heathlands that differ in their edaphic specificity, E. australis, sandstone specialist, and E. arborea, generalist. Average levels of within-population genetic diversity and gene flow between populations were significantly lower in Tangier (high sandstone patchiness) than in Algeciras (low patchiness) for the sandstone specialist, whereas no differences between both sides of the Strait were detected in the edaphic generalist. Since most endemic species in Mediterranean heathlands of the Strait of Gibraltar are sandstone specialists, these results highlight an increased vulnerability to loss of genetic diversity and local extinction of the heathland endemic flora in the Tangier side of the Strait of Gibraltar. © 2014 Gil-López et al.Project BREATHAL (CGL2011-28759/BOS) was financed by the Spanish Ministerio de Ciencia e Innovación.Peer Reviewe

Incomplete MaxSAT approaches for combinatorial testing

We present a Satisfiability (SAT)-based approach for building Mixed Covering Arrays with Constraints of minimum length, referred to as the Covering Array Number problem. This problem is central in Combinatorial Testing for the detection of system failures. In particular, we show how to apply Maximum Satisfiability (MaxSAT) technology by describing efficient encodings for different classes of complete and incomplete MaxSAT solvers to compute optimal and suboptimal solutions, respectively. Similarly, we show how to solve through MaxSAT technology a closely related problem, the Tuple Number problem, which we extend to incorporate constraints. For this problem, we additionally provide a new MaxSAT-based incomplete algorithm. The extensive experimental evaluation we carry out on the available Mixed Covering Arrays with Constraints benchmarks and the comparison with state-of-the-art tools confirm the good performance of our approaches.We would like to thank specially Akihisa Yamada for the access to several benchmarks for our experiments and for solving some questions about his previous work on Combinatorial Testing with Constraints. This work was partially supported by Grant PID2019-109137GB-C21 funded by MCIN/AEI/10.13039/501100011033, PANDEMIES 2020 by Agencia de Gestio d’Ajuts Universitaris i de Recerca (AGAUR), Departament d’Empresa i Coneixement de la Generalitat de Catalunya; FONDO SUPERA COVID-19 funded by Crue-CSIC-SANTANDER, ISINC (PID2019-111544GB-C21), and the MICNN FPU fellowship (FPU18/02929)

Total ozone measurements from the NDACC Izaña Subtropical Station: visible spectroscopy versus Brewer and satellite instruments

Póster presentado en: Quadrennial Ozone Symposium 2012 celebrado del 27 al 31 de agosto de 2012 en Toronto, CanadáThis work has been partially funded by NORS, UE FP7 Project under grant agreement n° 284421 and AMISOC, CGL2011‐24891, from the Spanish Plan for Research, Development and Innovation

On the concentration and size distribution of sub-micron aerosol in the Galápagos Islands

During the CHARLEX campaign in the Galápagos Islands, a Scanning Particle Mobility Sizer was deployed on San Cristobal Island in July-August 2011 to carry out size-resolved measurements of the concentration of submicron aerosols. To our knowledge these are the first measurements of aerosol concentrations in this unique environment. The particles with marine origin displayed a tri-modal number size distribution with peak diameters of 0.016 μm, 0.050 μm and 0.174 μm and a cloud-processed intermodal minimum at 0.093 μm. The mean total aerosol number concentration for the marine contribution was 470 ± 160 cm. A low particle concentration of 70 ± 50 cm for the nucleation size range was measured, but no evidence of new particle production in the atmospheric marine boundary layer (MBL) was observed. The concentration of the Aitken size mode was found to be related to aerosol entrainment from the free troposphere off the coast of Chile followed by transport within the MBL to the Galápagos Islands. Cloud processing may activate the particles in the Aitken size range, growing through 'in-cloud' sulphate production and increasing the particle concentration in the accumulation size range. The 0.093 μm cloud processed minima suggests that the critical supersaturation at which the particle is activated to a cloud droplet is in the 0.14-0.21% range. The daytime marine particle background concentration was influenced by human activity around the sampling site, as well as by new particle formation triggered by biogenic emissions from the vegetation cover of the island's semiarid lowlands. Effective CCN formation may play a role in the formation and properties of the stratus clouds, which permanently cover the top of the windward side of the islands and establish one of their characteristic climatic bands.Peer Reviewe

Long-path averaged mixing ratios of O₃ and NO₂ in the free troposphere from mountain MAX-DOAS

A new approximation is proposed to estimate O3 and NO2 mixing ratios in the northern subtropical free troposphere (FT). The proposed method uses O4 slant column densities (SCDs) at horizontal and near-zenith geometries to estimate a station-level differential path. The modified geometrical approach (MGA) is a simple method that takes advantage of a very long horizontal path to retrieve mixing ratios in the range of a few pptv. The methodology is presented, and the possible limitations are discussed. Multi-axis differential optical absorption spectroscopy (MAX-DOAS) high-mountain measurements recorded at the Izaña observatory (28° 18' N, 16° 29' W) are used in this study. The results show that under low aerosol loading, O3 and NO2 mixing ratios can be retrieved even at very low concentrations. The obtained mixing ratios are compared with those provided by in situ instrumentation at the observatory. The MGA reproduces the O3 mixing ratio measured by the in situ instrumentation with a difference of 28%. The different air masses scanned by each instrument are identified as a cause of the discrepancy between the O3 observed by MAX-DOAS and the in situ measurements. The NO2 is in the range of 20–40 ppt, which is below the detection limit of the in situ instrumentation, but it is in agreement with measurements from previous studies for similar conditions

Prescription refill, patient self-report and physician report in assessing adherence to oral endocrine therapy in early breast cancer patients: a restrospective cohort study in Catalonia, Spain.

AIMS: To compare different methods in order to assess adherence and persistence with oral endocrine therapy in women diagnosed with breast cancer (BC) in Catalonia. MATERIALS AND METHODS: This study covered all women newly diagnosed with stage I, II or IIIa BC and positive hormone receptors at six hospitals in Catalonia (Spain) in 2004. Adherence was assessed on the basis of physician report and patient self-report using a telephone questionnaire. Persistence was measured by refill prescriptions. We used the Kappa index to compare adherence measures and logistic regression to evaluate adherence-related risk factors. RESULTS: The study covered a total of 692 women. Adherence ranged from 92% (self-report) to 94.7% (physician report), depending on the measure used; persistence was 74.7% at 5 years of follow-up. Low concordance between measures was observed (Kappa range: 0.018-0.267). Patients aged 50-74 years showed higher adherence than those aged <50 years. Adherence was also associated with: adjuvant chemotherapy and sequential hormonal therapy. CONCLUSIONS: Concordance between the different measures was remarkably low, indicating the need for further research. Adherence is an issue in the management of BC patients taking oral drugs, and should be assessed in clinical practice

Spectral classification of the 100 pc white dwarf population from Gaia-DR3 and the virtual observatory

The third data release of Gaia has provided low resolution spectra for 100 000 white dwarfs (WDs) that, together with the excellent photometry and astrometry, represent an unrivalled benchmark for the study of this population. In this work, we first built a highly-complete volume-limited sample consisting in 12 718 WDs within 100 pc from the Sun. The use of VOSA tool allowed us to perform an automated fitting of their spectral energy distributions to different atmospheric models. In particular, the use of spectrally derived J-PAS photometry from Gaia spectra led to the classification of DA and non-DA WDs with an accuracy ¡ 90%, tested in already spectroscopically labelled objects. The excellent performance achieved was extended to practically the whole population of WDs with effective temperatures above 5500 K. Our results show that, while the A branch of the Gaia WD Hertzsprung-Russell diagram is practically populated by DA WDs, the B branch is largely formed by non-DAs (65%). The remaining 35% of DAs within the B branch implies a second peak at 0.8 M in the DA- mass distribution. Additionally, the Q branch and its extension to lower temperatures can be observed for both DA and non-DA objects due to core crystallisation. Finally, we derived a detailed spectral evolution function, which confirms a slow increase of the fraction of non-DAs as the effective temperature decreases down to 10 500 K, where it reaches a maximum of 36% and then decreases for lower temperatures down to 31%We are greatly indebted to Detlev Koester for providing us with his white dwarf model atmosphere spectra. ST acknowledges fruitful discussion and data provided by Carlos López-Sanjuan. We also thank to the referee Dr Jay Holberg for his valuable comments. FJE acknowledges financial support from the Servicio P ¨ublico de Empleo Estatal, Spain. PC acknowledges financial support from the Government of Comunidad Autónoma de Madrid (Spain) via postdoctoral grant ‘Atracción de Talento Investigador’ 2019-T2/TIC-14760. ST and ARM acknowledge support from MINECO under the PID2020-117252GB-I00 grant. ARM acknowledges support from Grant RYC-2016-20254 funded by MCIN/AEI/10.13039/501100011033 and by ESF Investing in your future. RMO is funded by INTA through grant PRE- OBSERVATORIO. MEC acknowledges support from NASA grants (HGC) 80NSSC17K0008 and (LWS) 80NSSC20K0193 and the University of Colorado Boulder.Peer ReviewedPostprint (published version

NO2 seasonal evolution in the north subtropical free troposphere

Three years of multi-axis differential optical absorption spectroscopy (MAXDOAS) measurements (2011-2013) have been used for estimating the NO2 mixing ratio along a horizontal line of sight from the high mountain subtropical observatory of Izaña, at 2370 m a.s.l. (NDACC station, 28.3° N, 16.5° W). The method is based on horizontal path calculation from the O2-O2 collisional complex at the 477 nm absorption band which is measured simultaneously to the NO2 column density, and is applicable under low aerosol-loading conditions. The MAXDOAS technique, applied in horizontal mode in the free troposphere, minimizes the impact of the NO2 contamination resulting from the arrival of marine boundary layer (MBL) air masses from thermally forced upwelling breeze during middle hours of the day. Comparisons with in situ observations show that during most of the measuring period, the MAXDOAS is insensitive or very slightly sensitive to the upwelling breeze. Exceptions are found for pollution events during southern wind conditions. On these occasions, evidence of fast, efficient and irreversible transport from the surface to the free troposphere is found. Background NO2 volume mixing ratio (vmr), representative of the remote free troposphere, is in the range of 20-45 pptv. The observed seasonal evolution shows an annual wave where the peak is in phase with the solar radiation. Model simulations with the chemistry-climate CAM-Chem model are in good agreement with the NO2 measurements, and are used to further investigate the possible drivers of the NO2 seasonality observed at Izaña.Peer Reviewe

NO2 climatology in the northern subtropical region: diurnal, seasonal and interannual variability

Daily NO2 vertical column density (VCD) has been routinely measured by zenith sky spectroscopy at the subtropical station of Izaña (28° N, 16° W) since 1993 in the framework of the Network for the Detection of Atmospheric Composition Change (NDACC). Based on 14 years of data the first low latitude NO2 VCD climatology has been established and the main characteristics from short timescales of one day to interannual variability are presented. Instrumental descriptions and different sources of errors are described in detail. The observed diurnal cycle follows that expected by gas-phase NOx chemistry, as can be shown by the good agreement with a vertically integrated chemical box model, and is modulated by solar radiation. The seasonal evolution departs from the phase of the hours of daylight, indicating the signature of upper stratospheric temperature changes. From the data record (1993–2006) no significant long-term trends in NO2 VCD can be inferred. Comparison of the ground-based data sets with nadir-viewing satellite spectrometers shows excellent agreement for SCIAMACHY with differences between both datasets of 1.1%. GOME displays unrealistic features with the largest discrepancies during summer. The ground-based data are compared with long-term output of the SLIMCAT 3-D chemical transport model (CTM). The basic model, forced by ECMWF (ERA-40) analyses, captures the observed NO2 annual cycle but significantly underestimates the spring/summer maximum (by 12% at sunset and up to 25% at sunrise). In a model run which uses assimilation of satellite CH4 profiles to constrain the model long-lived tracers the agreement is significantly improved. This improvement in modelled column NO2 is due to better modelled NOy profiles and points to transport errors in the ECMWF ERA-40 reanalyses.Routine measurements have been possible thanks to the funding provided by the projects QUILT (EU EVK2-CT2000-0059) SCOUT-O3 (EU 505390-GOCE-CT-2004), TROMPETA (MCyT CGL2004-03669) and is on-going through GEOMON (EU FP6-2005-Global-4- 036677)

Intake of slow-digesting carbohydrates is related to changes in the microbiome and its functional pathways in growing rats with obesity induced by diet

Introduction: The main cause of insulin resistance in childhood is obesity, which contributes to future comorbidities as in adults. Although high-calorie diets and lack of exercise contribute to metabolic disease development, food quality rather than the quantity of macronutrients is more important than food density. The purpose of the present study was to examine the effects of changing the quality of carbohydrates from rapidly to slowly digestible carbohydrates on the composition of the gut microbiota and the profiles of the functional pathways in growing rats with obesity due to a high-fat diet (HFD). Methods: During the course of 4 weeks, rats growing on an HFD-containing carbohydrates with different digestive rates were fed either HFD-containing carbohydrates with a rapid digestion rate (OBE group) or HFD-containing carbohydrates with a slow digestion rate (OBE-ISR group). A non-obese group (NOB) was included as a reference, and rats were fed on a rodent standard diet (AIN93G). An analysis of gut microbiota was conducted using 16S rRNA-based metagenomics; a linear mixed-effects model (LMM) was used to determine changes in abundance between baseline and 4 weeks of treatment, and functional pathways were identified. Gut microbiota composition at bacterial diversity and relative abundance, at phylum and genus levels, and functional profiles were analyzed by integrating the Integrated Microbial Genomes (IMG) database. Results: The groups showed comparable gut microbiota at baseline. At the end of the treatment, animals from the ISR group exhibited differences at the phylum levels by decreasing the diversity of Fisher’s index and Firmicutes (newly named as Bacillota), and increasing the Pielou’s evenness and Bacteroidetes (newly named as Bacteroidota); at the genus level by increasing Alistipes, Bifidobacterium, Bacteroides, Butyricimonas, Lachnoclostridium, Flavonifractor, Ruminiclostridium 5, and Faecalibaculum and decreasing Muribaculum, Blautia, and Ruminiclostridium 9. Remarkably, relative abundances of genera Tyzzerella and Angelakisella were higher in the OBE group compared to NOB and OBE-ISR groups. In addition, some microbiota carbohydrate metabolism pathways such as glycolysis, glucuronic acid degradation, pentose phosphate pathway, methanogenesis, and fatty acid biosynthesis exhibited increased activity in the OBE-ISR group after the treatment. Higher levels of acetate and propionate were found in the feces of the ISR group compared with the NOB and OBE groups. Conclusion: The results of this study demonstrate that replacing rapidly digestible carbohydrates with slowly digestible carbohydrates within an HFD improve the composition of the gut microbiota. Consequently, metabolic disturbances associated with obesity may be prevented.Abbott Laboratories S.A"Fundacion Ramon Areces", Madrid, Spai