Support immunologique pour biocapteur : caractérisations physico-chimiques et biologiques

The aim of my PhD thesis, conducted as part of a collaboration between the laboratory of protein separation sciences and nanotechnology (Paris-Sud Galen Institute) and the Micro and Nano System (basic electronics institute) group was to study the influence of self-assembled monolayers on the biological activity of bioreceptor toward biosensor development. In this project, we choose the organosilanes that can bind covalently to the silicon. Two silanes (7-octenyl trichlorosilane(OTS) and 3- aminopropyltriethoxysilane (APTES)) were studied., Their impact on the stability and the functionality of bio- receptor , model mouse immunoglobulin G (IgG), were evaluated. Spectroscopic characterization by XPS and infra- red Fourier transformed (FTIR) was first carried out to assess that the silanized surface exhibit carboxylic groups. The homogeneity of the surfaces was measured by AFM. Then, IgG were immobilized on these supports, covalently and a topographic AFM study was conducted to measure the distribution of these antibodies. The orientation of the grafted antibody was investigated by immune-enzymatic assays. We have also evaluated the binding capacity of the IgG immobilized on both surfaces. Then, the impact of aging on APTES surface was evaluated by spectroscopics and biological methods.L’objectif de mon doctorat, réalisé dans le cadre d’une collaboration entre le laboratoire des protéines et nanotechnologies en sciences séparatives (institut Galien Paris-Sud) et le groupe Micro et Nano Système (institut d’électronique fondamentale) était d’étudier l’influence des monocouches autoassemblées, sur l’activité biologique du bio-récepteur dans une perspective de développement de biocapteur. Dans ce projet, nous avons choisi les organo-silanes qui peuvent se lier de manière covalente sur le silicium. Deux silanes (7-octenyl trichlorosilanes et le (3-aminopropyl)triethoxysilane) ont été étudiés, leur impact en terme de nature et de stabilité sur la fonctionnalité du bio-récepteur, des immunoglobulines G de souris, ont été évalué. Dans un premier temps, nous nous sommes intéressés à la fonctionnalité des anticorps greffés sur une mono couche auto-assemblée composée de 7-octenyltrichlorosilane (OTS) présentant à sa surface un groupement carboxylique. Une caractérisation spectroscopique par XPS et infra-rouge à transformé de Fourrier (FTIR) a tout d’abord été effectuée afin de vérifier la présence de ces groupements carboxyliques. L’homogénéité de la surface a été évaluée par AFM. Nous avons ensuite immobilisé ces anticorps, sur ces supports, de manière covalente et une étude topographique par AFM a été menée pour mesurer la répartition de ces anticorps. L’orientation des anticorps greffés a été évaluée à l’aide d’immuno-essais. Ensuite, nous avons comparé l’APTES, permettant l’obtention de plaques de silicium fonctionnalisé avec des groupements aminés à leur surface, avec l’OTS. Nous avons notamment comparé la capacité de capture des anticorps immobilisés sur ces deux types de silanes. Dans la dernière partie, l’impact du vieillissement d’un support immunologique préparé chimiquement en utilisant l’APTES a été évaluée sur le plan physico-chimique et biologique

Fe-MCM-22 zeolites: synthesis and study about the states of iron

The Fe-MCM-22 zeolite was successfully synthesized with hexametylenimine template. Several physicochemical techniques (XRD, SEM, BET, AAS, IR and ESR) have been used to characterize this zeolite. Iron exists under three states: isolated ions in tetrahedral lattice positions, in octahedral coordination as isolated ions at cationic positions and as aggregated oxide species or hydroxide phases.Keywords: Fe-MCM-22 zeolite, synthesis, characterization, framework iron

COPPER-MODIFIED MCM-22 AS CATALYSTS FOR HYDROCARBON SELECTIVE CATALYTIC REDUCTION OF NOX

Joint Research on Environmental Science and Technology for the Eart

An Outbreak of Severe Infections with Community-Acquired MRSA Carrying the Panton-Valentine Leukocidin Following Vaccination

Background: Infections with community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) are emerging worldwide. We investigated an outbreak of severe CA-MRSA infections in children following out-patient vaccination. Methods and Findings: We carried out a field investigation after adverse events following immunization (AEFI) were reported. We reviewed the clinical data from all cases. S. aureus recovered from skin infections and from nasal and throat swabs were analyzed by pulse-field gel electrophoresis, multi locus sequence typing, PCR and microarray. In May 2006, nine children presented with AEFI, ranging from fatal toxic shock syndrome, necrotizing soft tissue infection, purulent abscesses, to fever with rash. All had received a vaccination injection in different health centres in one District of Ho Chi Minh City. Eight children had been vaccinated by the same health care worker (HCW). Deficiencies in vaccine quality, storage practices, or preparation and delivery were not found. Infection control practices were insufficient. CA-MRSA was cultured in four children and from nasal and throat swabs from the HCW. Strains from children and HCW were indistinguishable. All carried the Panton-Valentine leukocidine (PVL), the staphylococcal enterotoxin B gene, the gene complex for staphylococcal-cassette-chromosome mec type V, and were sequence type 59. Strain HCM3A is epidemiologically unrelated to a strain of ST59 prevalent in the USA, althoughthey belong to the same lineage. Conclusions. We describe an outbreak of infections with CA-MRSA in children, transmitted by an asymptomatic colonized HCW during immunization injection. Consistent adherence to injection practice guidelines is needed to prevent CA-MRSA transmission in both in- and outpatient settings

Safety and efficacy of fluoxetine on functional outcome after acute stroke (AFFINITY): a randomised, double-blind, placebo-controlled trial

Background Trials of fluoxetine for recovery after stroke report conflicting results. The Assessment oF FluoxetINe In sTroke recoverY (AFFINITY) trial aimed to show if daily oral fluoxetine for 6 months after stroke improves functional outcome in an ethnically diverse population. Methods AFFINITY was a randomised, parallel-group, double-blind, placebo-controlled trial done in 43 hospital stroke units in Australia (n=29), New Zealand (four), and Vietnam (ten). Eligible patients were adults (aged ≥18 years) with a clinical diagnosis of acute stroke in the previous 2–15 days, brain imaging consistent with ischaemic or haemorrhagic stroke, and a persisting neurological deficit that produced a modified Rankin Scale (mRS) score of 1 or more. Patients were randomly assigned 1:1 via a web-based system using a minimisation algorithm to once daily, oral fluoxetine 20 mg capsules or matching placebo for 6 months. Patients, carers, investigators, and outcome assessors were masked to the treatment allocation. The primary outcome was functional status, measured by the mRS, at 6 months. The primary analysis was an ordinal logistic regression of the mRS at 6 months, adjusted for minimisation variables. Primary and safety analyses were done according to the patient's treatment allocation. The trial is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12611000774921. Findings Between Jan 11, 2013, and June 30, 2019, 1280 patients were recruited in Australia (n=532), New Zealand (n=42), and Vietnam (n=706), of whom 642 were randomly assigned to fluoxetine and 638 were randomly assigned to placebo. Mean duration of trial treatment was 167 days (SD 48·1). At 6 months, mRS data were available in 624 (97%) patients in the fluoxetine group and 632 (99%) in the placebo group. The distribution of mRS categories was similar in the fluoxetine and placebo groups (adjusted common odds ratio 0·94, 95% CI 0·76–1·15; p=0·53). Compared with patients in the placebo group, patients in the fluoxetine group had more falls (20 [3%] vs seven [1%]; p=0·018), bone fractures (19 [3%] vs six [1%]; p=0·014), and epileptic seizures (ten [2%] vs two [<1%]; p=0·038) at 6 months. Interpretation Oral fluoxetine 20 mg daily for 6 months after acute stroke did not improve functional outcome and increased the risk of falls, bone fractures, and epileptic seizures. These results do not support the use of fluoxetine to improve functional outcome after stroke

Immune support for biosensor : physico-chemical and biological characterization

L’objectif de mon doctorat, réalisé dans le cadre d’une collaboration entre le laboratoire des protéines et nanotechnologies en sciences séparatives (institut Galien Paris-Sud) et le groupe Micro et Nano Système (institut d’électronique fondamentale) était d’étudier l’influence des monocouches autoassemblées, sur l’activité biologique du bio-récepteur dans une perspective de développement de biocapteur. Dans ce projet, nous avons choisi les organo-silanes qui peuvent se lier de manière covalente sur le silicium. Deux silanes (7-octenyl trichlorosilanes et le (3-aminopropyl)triethoxysilane) ont été étudiés, leur impact en terme de nature et de stabilité sur la fonctionnalité du bio-récepteur, des immunoglobulines G de souris, ont été évalué. Dans un premier temps, nous nous sommes intéressés à la fonctionnalité des anticorps greffés sur une mono couche auto-assemblée composée de 7-octenyltrichlorosilane (OTS) présentant à sa surface un groupement carboxylique. Une caractérisation spectroscopique par XPS et infra-rouge à transformé de Fourrier (FTIR) a tout d’abord été effectuée afin de vérifier la présence de ces groupements carboxyliques. L’homogénéité de la surface a été évaluée par AFM. Nous avons ensuite immobilisé ces anticorps, sur ces supports, de manière covalente et une étude topographique par AFM a été menée pour mesurer la répartition de ces anticorps. L’orientation des anticorps greffés a été évaluée à l’aide d’immuno-essais. Ensuite, nous avons comparé l’APTES, permettant l’obtention de plaques de silicium fonctionnalisé avec des groupements aminés à leur surface, avec l’OTS. Nous avons notamment comparé la capacité de capture des anticorps immobilisés sur ces deux types de silanes. Dans la dernière partie, l’impact du vieillissement d’un support immunologique préparé chimiquement en utilisant l’APTES a été évaluée sur le plan physico-chimique et biologique.The aim of my PhD thesis, conducted as part of a collaboration between the laboratory of protein separation sciences and nanotechnology (Paris-Sud Galen Institute) and the Micro and Nano System (basic electronics institute) group was to study the influence of self-assembled monolayers on the biological activity of bioreceptor toward biosensor development. In this project, we choose the organosilanes that can bind covalently to the silicon. Two silanes (7-octenyl trichlorosilane(OTS) and 3- aminopropyltriethoxysilane (APTES)) were studied., Their impact on the stability and the functionality of bio- receptor , model mouse immunoglobulin G (IgG), were evaluated. Spectroscopic characterization by XPS and infra- red Fourier transformed (FTIR) was first carried out to assess that the silanized surface exhibit carboxylic groups. The homogeneity of the surfaces was measured by AFM. Then, IgG were immobilized on these supports, covalently and a topographic AFM study was conducted to measure the distribution of these antibodies. The orientation of the grafted antibody was investigated by immune-enzymatic assays. We have also evaluated the binding capacity of the IgG immobilized on both surfaces. Then, the impact of aging on APTES surface was evaluated by spectroscopics and biological methods

Support immunologique pour biocapteur (caractérisations physico-chimiques et biologiques)

L objectif de mon doctorat, réalisé dans le cadre d une collaboration entre le laboratoire des protéines et nanotechnologies en sciences séparatives (institut Galien Paris-Sud) et le groupe Micro et Nano Système (institut d électronique fondamentale) était d étudier l influence des monocouches autoassemblées, sur l activité biologique du bio-récepteur dans une perspective de développement de biocapteur. Dans ce projet, nous avons choisi les organo-silanes qui peuvent se lier de manière covalente sur le silicium. Deux silanes (7-octenyl trichlorosilanes et le (3-aminopropyl)triethoxysilane) ont été étudiés, leur impact en terme de nature et de stabilité sur la fonctionnalité du bio-récepteur, des immunoglobulines G de souris, ont été évalué. Dans un premier temps, nous nous sommes intéressés à la fonctionnalité des anticorps greffés sur une mono couche auto-assemblée composée de 7-octenyltrichlorosilane (OTS) présentant à sa surface un groupement carboxylique. Une caractérisation spectroscopique par XPS et infra-rouge à transformé de Fourrier (FTIR) a tout d abord été effectuée afin de vérifier la présence de ces groupements carboxyliques. L homogénéité de la surface a été évaluée par AFM. Nous avons ensuite immobilisé ces anticorps, sur ces supports, de manière covalente et une étude topographique par AFM a été menée pour mesurer la répartition de ces anticorps. L orientation des anticorps greffés a été évaluée à l aide d immuno-essais. Ensuite, nous avons comparé l APTES, permettant l obtention de plaques de silicium fonctionnalisé avec des groupements aminés à leur surface, avec l OTS. Nous avons notamment comparé la capacité de capture des anticorps immobilisés sur ces deux types de silanes. Dans la dernière partie, l impact du vieillissement d un support immunologique préparé chimiquement en utilisant l APTES a été évaluée sur le plan physico-chimique et biologique.The aim of my PhD thesis, conducted as part of a collaboration between the laboratory of protein separation sciences and nanotechnology (Paris-Sud Galen Institute) and the Micro and Nano System (basic electronics institute) group was to study the influence of self-assembled monolayers on the biological activity of bioreceptor toward biosensor development. In this project, we choose the organosilanes that can bind covalently to the silicon. Two silanes (7-octenyl trichlorosilane(OTS) and 3- aminopropyltriethoxysilane (APTES)) were studied., Their impact on the stability and the functionality of bio- receptor , model mouse immunoglobulin G (IgG), were evaluated. Spectroscopic characterization by XPS and infra- red Fourier transformed (FTIR) was first carried out to assess that the silanized surface exhibit carboxylic groups. The homogeneity of the surfaces was measured by AFM. Then, IgG were immobilized on these supports, covalently and a topographic AFM study was conducted to measure the distribution of these antibodies. The orientation of the grafted antibody was investigated by immune-enzymatic assays. We have also evaluated the binding capacity of the IgG immobilized on both surfaces. Then, the impact of aging on APTES surface was evaluated by spectroscopics and biological methods.PARIS11-SCD-Bib. électronique (914719901) / SudocSudocFranceF

#256 : Reproductive Outcomes and Satisfaction Among Patients Using Telemedicine During Assisted Reproduction: A Meta-Analysis

Background and Aims: Telemedicine refers to the delivery of healthcare services through the use of information and communication technology, across distances. There has been a significant increase in the demand for infertility treatment and virtual consultations from home after the COVID-19 pandemic. Despite this, due to a lack of sufficient data, and concerns about patient compliance, telemedicine has struggled to gain widespread acceptance. Our aim was to compare the success of treatment between telemedicine and in-office care during assisted reproduction treatment. Additionally, we evaluated patient satisfaction with telemedicine. Method: We conducted a literature search of published articles in the PubMed/MEDLINE, EMBASE, Scopus, and Web of Science databases up to November 30, 2022. We used the keywords “telemedicine” or “telehealth” and “in vitro fertilization” or “assisted reproduction”. Both randomized controlled trials and observational studies were included in the search. We registered our protocol on PROSPERO (Registration number: CRD42022379428). Results: We identified 14 studies that reported data on 5182 patients. Quality assessment revealed an acceptable risk of bias for both randomized controlled trials and observational studies. The data showed that telemedicine was non-inferior to in-person care in terms of pregnancy rate achieved (OR 1.02, 95% CI: 0.83–1.25, p = 0.84). The Q test p-value of 0.99 and I2 statistic of 0% indicated that all the included studies were homogeneous. Patients who received telemedicine follow-ups during fertility treatment reported adequate satisfaction (88%, 95% CI: 77%-94%). Eggers’ test did not indicate the presence of Deek’s funnel plot asymmetry (p = 0.62), confirming that no publication bias was found. Conclusion: Telemedicine use has resulted in a comparable treatment success rate in terms of pregnancy rate, when compared to face-to-face consultation. These emerging tools also yielded a high level of patient satisfaction