FRENCH QUALITY AND ECO-LABELING SCHEMES: DO THEY ALSO BENEFIT THE ENVIRONMENT?

The environmental effects of various 'quality' and 'eco-labeling' programs in the Midi-Pyrenees region of the south of France are analyzed, using factor analysis, analysis of variance, and qualitative analysis. Implications for agri-environmental policies on both sides of the Atlantic are discussed.Environmental Economics and Policy,

A Behavioral Approach to Understanding the Git Experience

The Investigating and Archiving the Scholarly Git Experience (IASGE) project is multi-track study focused on understanding the uses of Git by students, faculty, and staff working in academic research institutions as well as the ways source code repositories and their associated contextual ephemera can be better preserved. This research, in turn, has implications regarding how to support Git in the scholarly process, how version control systems contribute to reproducibility, and how Library and Information Science (LIS) professionals can support Git through instruction and sustainability efforts. In this paper, we focus on a subset of our larger project and take a deep look at what code hosting platforms offer researchers in terms of productivity and collaboration. For this portion, a survey, focus groups, and user experience interviews were conducted to gain an understanding of how and why scholarly researchers use Version Control Systems (VCS) as well as some of the pain points in learning and using VCS for daily work

Synthetic protein-conductive membrane nanopores built with DNA

Nanopores are key in portable sequencing and research given their ability to transport elongated DNA or small bioactive molecules through narrow transmembrane channels. Transport of folded proteins could lead to similar scientific and technological benefits. Yet this has not been realised due to the shortage of wide and structurally defined natural pores. Here we report that a synthetic nanopore designed via DNA nanotechnology can accommodate folded proteins. Transport of fluorescent proteins through single pores is kinetically analysed using massively parallel optical readout with transparent silicon-on-insulator cavity chips vs. electrical recordings to reveal an at least 20-fold higher speed for the electrically driven movement. Pores nevertheless allow a high diffusive flux of more than 66 molecules per second that can also be directed beyond equillibria. The pores may be exploited to sense diagnostically relevant proteins with portable analysis technology, to create molecular gates for drug delivery, or to build synthetic cells

Rational design of an estrogen receptor mutant with altered DNA-binding specificity

Although artificial C2-H2 zinc fingers can be designed to recognize specific DNA sequences, it remains unclear to which extent nuclear receptor C4 zinc fingers can be tailored to bind novel DNA elements. Steroid receptors bind as dimers to palindromic response elements differing in the two central base pairs of repeated motifs. Predictions based on one amino acid—one base-pair relationships may not apply to estrogen receptors (ERs), which recognize the two central base pairs of estrogen response elements (EREs) via two charged amino acids, each contacting two bases on opposite DNA strands. Mutagenesis of these residues, E203 and K210 in ERα, indicated that both contribute to ERE binding. Removal of the electric charge and steric constraints associated with K210 was required for full loss of parental DNA-binding specificity and recognition of novel sequences by E203 mutants. Although some of the new binding profiles did not match predictions, the double mutation E203R-K210A generated as predicted a mutant ER that was transcriptionally active on palindromes of PuGCTCA motifs, but not on consensus EREs. This study demonstrates the feasibility of designing C4 zinc finger mutants with novel DNA-binding specificity, but also uncovers limitations of this approach

The ZERO: Zip-Tie Revolver

The Boise State University Microgravity Team has been challenged with designing a mechanical Zip-tie tool to be used during EVA missions on the ISS. NASA is looking for a new tool that will allow astronauts to fasten zip-ties to secure wires and hoses. This tool would need to have a number of zip-ties stored internally, be quickly reusable, and would need to be easily and safely operated by an astronaut in a single handed operation

Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities.

The mammary epithelium depends on specific lineages and their stem and progenitor function to accommodate hormone-triggered physiological demands in the adult female. Perturbations of these lineages underpin breast cancer risk, yet our understanding of normal mammary cell composition is incomplete. Here, we build a multimodal resource for the adult gland through comprehensive profiling of primary cell epigenomes, transcriptomes, and proteomes. We define systems-level relationships between chromatin-DNA-RNA-protein states, identify lineage-specific DNA methylation of transcription factor binding sites, and pinpoint proteins underlying progesterone responsiveness. Comparative proteomics of estrogen and progesterone receptor-positive and -negative cell populations, extensive target validation, and drug testing lead to discovery of stem and progenitor cell vulnerabilities. Top epigenetic drugs exert cytostatic effects; prevent adult mammary cell expansion, clonogenicity, and mammopoiesis; and deplete stem cell frequency. Select drugs also abrogate human breast progenitor cell activity in normal and high-risk patient samples. This integrative computational and functional study provides fundamental insight into mammary lineage and stem cell biology

Testing peatland testate amoeba transfer functions: Appropriate methods for clustered training-sets

Transfer functions are widely used in palaeoecology to infer past environmental conditions from fossil remains of many groups of organisms. In contrast to traditional training-set design with one observation per site, some training-sets, including those for peatland testate amoeba-hydrology transfer functions, have a clustered structure with many observations from each site. Here we show that this clustered design causes standard performance statistics to be overly optimistic. Model performance when applied to independent data sets is considerably weaker than suggested by statistical cross-validation. We discuss the reasons for these problems and describe leave-one-site-out cross-validation and the cluster bootstrap as appropriate methods for clustered training-sets. Using these methods we show that the performance of most testate amoeba-hydrology transfer functions is worse than previously assumed and reconstructions are more uncertain

Influence of the characteristics of the house and place of residence in the daily educational activities of children during the period of COVID-19’ confinement

The period of confinement motivated by the COVID-19 pandemic and established by the governments of different countries has influenced the lifestyle of millions of children, not being able to continue carrying out multiple educational activities as they did until confinement. The objective of this research was to determine and analyze whether the living conditions of children during the period of confinement caused by COVID-19 influenced their daily educational activities. A descriptive, comparative and cross- sectional quantitative study with a non-experimental design was carried out, with a single measurement in a single group. Factors associate with living conditions were analyzed, such as the place of residence and the type of house in which Spanish children have been confined, as well as the number and use of technological devices. The parents' perception of the children's state of fatigue, happiness, energy and tiredness was also analyzed. We have worked with a sample of 837 Spanish children. As a data collection instrument, the validated questionnaire on Equipment and Use of Information and Communication Technologies in Households (TIC-H2019) of the National Statistics Institute (INE) was used, following the recommendations of the Statistical Office of the European Union (EUROSTAT). The results confirm some statistically significant influence of the conditions of the house and place of residence on the daily time dedicated to different educational activities such as reading, physical activity, free play or use of technological devices between children residing in small flats and those residing in large flats or houses with garden and those residing in urban and rural settings

C-Terminal Domain Deletion Enhances the Protective Activity of cpa/cpb Loaded Solid Lipid Nanoparticles against Leishmania major in BALB/c Mice

Cutaneous leishmaniasis (CL) is the most common form of leishmaniasis with an annual incidence of approximately 2 million cases and is endemic in 88 countries, including Iran. CL's continued spread, along with rather ineffectual treatments and drug-resistant variants emergence has increased the need for advanced preventive strategies. We studied Type II cysteine proteinase (CPA) and Type I (CPB) with its C-terminal extension (CTE) as cocktail DNA vaccine against murine and canine leishmaniasis. However, adjuvants' success in enhancing immune responses to selected antigens led us to refocus our vaccine development programs. Herein, we discuss cationic solid lipid nanoparticles' (cSLN) ability to improve vaccine-induced protective efficacy against CL and subsequent lesion size and parasite load reduction in BALB/c mice. For this work, we evaluated five different conventional as well as novel parasite detection techniques, i.e., footpad imaging, footpad flowcytometry and lymph node flowcytometry for disease progression assessments. Vaccination with cSLN-cpa/cpb-CTE formulation showed highest parasite inhibition at 3-month post vaccination. Immunized mice showed reduced IL-5 level and significant IFN-ã increase, compared to control groups. We think our study represents a potential future and a major step forward in vaccine development against leishmaniasis