Chronic Stress Triggers Expression of Immediate Early Genes and Differentially Affects the Expression of AMPA and NMDA Subunits in Dorsal and Ventral Hippocampus of Rats

Indexación: Web of Science; Scopus.Previous studies in rats have demonstrated that chronic restraint stress triggers anhedonia, depressive-like behaviors, anxiety and a reduction in dendritic spine density in hippocampal neurons. In this study, we compared the effect of repeated stress on the expression of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) and N-methyl-D-aspartate (NMDA) receptor subunits in dorsal and ventral hippocampus (VH). Adult male Sprague-Dawley rats were randomly divided into control and stressed groups, and were daily restrained in their motion (2.5 h/day) during 14 days. We found that chronic stress promotes an increase in c-Fos mRNA levels in both hippocampal areas, although it was observed a reduction in the immunoreactivity at pyramidal cell layer. Furthermore, Arc mRNAs levels were increased in both dorsal and VH, accompanied by an increase in Arc immunoreactivity in dendritic hippocampal layers. Furthermore, stress triggered a reduction in PSD-95 and NR1 protein levels in whole extract of dorsal and VH. Moreover, a reduction in NR2A/NR2B ratio was observed only in dorsal pole. In synaptosomal fractions, we detected a rise in NR1 in dorsal hippocampus (DH). By indirect immunofluorescence we found that NR1 subunits rise, especially in neuropil areas of dorsal, but not VH. In relation to AMPA receptor (AMPAR) subunits, chronic stress did not trigger any change, either in dorsal or ventral hippocampal areas. These data suggest that DH is more sensitive than VH to chronic stress exposure, mainly altering the expression of NMDA receptor (NMDAR) subunits, and probably favors changes in the configuration of this receptor that may influence the function of this area.https://www.frontiersin.org/articles/10.3389/fnmol.2017.00244/ful

Validation and Adaptation of the Prosocial and Antisocial Behavior in Sport Scale to the Spanish context of Physical Education

Physical education (PE), by its own characteristics, is a subject where social communication is especially promoted. However, it is necessary to have tools that evaluate the social behaviour of students during PE classes. For this reason, we propose to validate and adapt the Prosocial and Antisocial Behavior in Sport Scale to the Spanish context of PE classes. The study involved 1081 students aged 12 to 18 (M = 14.83; SD = 1.27). The psychometric properties of the Prosocial Behavior Scale were analyzed through several statistical analyses. The results of the confirmatory factorial analysis and the exploratory factorial analysis supported the internal structure of the questionnaire. In addition, the scale was invariant to gender. Cronbach’s alpha values were higher than 0.70 in the factors and sub-factors, finally showing adequate levels of temporal stability. Taking into account the results achieved in the present study, PE teachers have an effective tool to assess the social and antisocial behaviour of their students’ students during PE classes

Electrostatic potential variations on stellarator magnetic surfaces in low collisionality regimes

The component of the neoclassical electrostatic potential that is non-constant on the magnetic surface, that we denote by $\tilde\varphi$, can affect radial transport of highly charged impurities, and this has motivated its inclusion in some modern neoclassical codes. The number of neoclassical simulations in which $\tilde\varphi$ is calculated is still scarce, partly because they are usually demanding in terms of computational resources, especially at low collisionality. In this paper the size, the scaling with collisionality and with aspect ratio, and the structure of $\tilde\varphi$ on the magnetic surface are analytically derived in the $1/\nu$, $\sqrt{\nu}$ and superbanana-plateau regimes of stellarators close to omnigeneity; i. e. stellarators that have been optimized for neoclassical transport. It is found that the largest $\tilde\varphi$ that the neoclassical equations admit scales linearly with the inverse aspect ratio and with the size of the deviation from omnigeneity. Using a model for a perturbed omnigeneous configuration, the analytical results are verified and illustrated with calculations by the code KNOSOS. The techniques, results and numerical tools employed in this paper can be applied to neoclassical transport problems in tokamaks with broken axisymmetry.Comment: 30 pages, 12 figures, 1 table. Published versio

Utilización de la música en educación física: principales problemas

La utilización de la música para el desarrollo de las clases de Educación Física nos puede ofrecer multitud de posibilidades, convirtiéndose en un elemento indispensable para alcanzar objetivos didácticos. Pero la realidad docente está muy lejos de aprovechar el potencial que la música ofrece, tendiendo a un escaso uso, que casi siempre se cierne al contenido de Expresión Corporal, actualmente denominado en los contenidos oficiales, Ritmo y Expresión. Hemos realizado un estudio cuyo objetivo es conocer como el profesor de Educación Física utiliza la música en sus clases y cuales son los principales motivos que causan tal deficiencia. Para obtener los datos aplicamos un cuestionario de opinión que fue contestado por el 95% de los profesores de Educación Física que imparten clase en primer ciclo de la E.S.O. de la Región de Murcia. De los resultados obtenidos podemos extraer que aunque el profesor tiene una valoración positiva sobre la música, el principal factor que influye en su falta de utilización es la escasa formación que ha recibido durante sus estudios, tanto a nivel inicial como permanente

The ROCK inhibitor Fasudil prevents chronic restraint stress-induced depressive-like behaviors and dendritic spine loss in rat hippocampus

Indexación: Web of Science; Scopus.Background: Dendritic arbor simplification and dendritic spine loss in the hippocampus, a limbic structure implicated in mood disorders, are assumed to contribute to symptoms of depression. These morphological changes imply modifications in dendritic cytoskeleton. Rho GTPases are regulators of actin dynamics through their effector Rho kinase. We have reported that chronic stress promotes depressive-like behaviors in rats along with dendritic spine loss in apical dendrites of hippocampal pyramidal neurons, changes associated with Rho kinase activation. The present study proposes that the Rho kinase inhibitor Fasudil may prevent the stress-induced behavior and dendritic spine loss. Methods: Adult male Sprague-Dawley rats were injected with saline or Fasudil (i.p., 10 mg/kg) starting 4 days prior to and maintained during the restraint stress procedure (2.5 h/d for 14 days). Nonstressed control animals were injected with saline or Fasudil for 18 days. At 24 hours after treatment, forced swimming test, Golgi-staining, and immuno-western blot were performed. Results: Fasudil prevented stress-induced immobility observed in the forced swimming test. On the other hand, Fasudiltreated control animals showed behavioral patterns similar to those of saline-treated controls. Furthermore, we observed that stress induced an increase in the phosphorylation of MYPT1 in the hippocampus, an exclusive target of Rho kinase. This change was accompanied by dendritic spine loss of apical dendrites of pyramidal hippocampal neurons. Interestingly, increased pMYPT1 levels and spine loss were both prevented by Fasudil administration. Conclusion: Our findings suggest that Fasudil may prevent the development of abnormal behavior and spine loss induced by chronic stress by blocking Rho kinase activity.https://academic.oup.com/ijnp/article/20/4/336/263217

LoRa-based Network for Water Quality Monitoring in Coastal Areas

[EN] Agriculture Farming activity near to rivers and coastal areas sometimes imply spills of chemical and fertilizers products in aquifers and rivers. These spill highly affect the water quality in rivers' mouths and beaches close to those rivers. The presence of these elements can worse the quality for its normal use, even for its enjoying. When this polluted water reaches the sea can also have problematic consequences for fauna and flora. For this reason, it is important to rapidly detect where these spills are taking place and where the water does not have the minimum of quality to be used. In this article we propose the design and implementation of a LoRa (Long Range) based wireless sensor network for monitoring the quality of water in coastal areas, rivers and ditches with the aim to generate an observatory of water quality of the monitored areas. This network is composed by several wireless sensor nodes endowed with several sensors to physically measure parameters of water quality, such as turbidity, temperature, etc., and weather conditions such as temperature and relative humidity. The data collected by the sensors is sent to a gateway that forwards them to our storage database. The database is used to create an observatory that will permit the monitoring of the environment where the network is deployed. We test different devices to select the one that presents the best performance. Finally, the final solution is tested in a real environment for checking its correct operation. Two different tests will be carried out. The first test checks the correct operation of sensors and the network architecture while the second test show us the devices performance in terms of coverage.Sendra, S.; Parra-Boronat, L.; Jimenez, JM.; García-García, L.; Lloret, J. (2023). LoRa-based Network for Water Quality Monitoring in Coastal Areas. Mobile Networks and Applications (Online). 28(1):65-81. https://doi.org/10.1007/s11036-022-01994-8658128

A methodological proposal and tool support for the HL7 standards compliance in the development of health information systems

Health information systems are increasingly complex, and their development is presented as a challenge for software development companies offering quality, maintainable and interoperable products. HL7 (Health level 7) International, an international non-profit organization, defines and maintains standards related to health information systems. However, the modelling languages proposed by HL7 are far removed from standard languages and widely known by software engineers. In these lines, NDT is a software development methodology that has a support tool called NDT-Suite and is based, on the one hand, on the paradigm of model-driven engineering and, on the other hand, in UML that is a widely recognized standard language. This paper proposes an extension of the NDT methodology called MoDHE (Model Driven Health Engineering) to offer software engineers a methodology capable of modelling health information systems conforming to HL7 using UML domain models