Influencia de las aplicaciones hormonales para el retraso de la recolección en el caqui 'Rojo Brillante' sobre la brotación, desarrollo vegetativo y floración. Técnicas de estímulo

[ES] Tras el retraso de la recolección del caqui promovido por la aplicación de ácido giberélico y sales de calcio, se ha observado un decaimiento generalizado en el vigor del árbol que repercute negativamente en la brotación y floración siguiente y, por consiguiente, en la cosecha. El objetivo de este trabajo es estudiar si este declive en el crecimiento vegetativo puede recuperarse con la aplicación de la hormona y compuestos nitrogenados y cuál es su comportamiento nutricional. Para ello, se ha realizado un seguimiento del crecimiento vegetativo de 5 brotes en cada uno de los 20 árboles, siendo 10 de estos tratados en otoño (30 de septiembre, 24 de octubre y 8 de noviembre) con AG para retrasar la recolección. A una parte de estos árboles se les aplicó adicionalmente en el momento de la brotación (28 de marzo) una mezcla de AG(2.5g) y un compuesto nitrogenado (28% N), y también a otros de los que en otoño habían sido control, y se repitió 35 días después (2 de mayo). Siete árboles se mantuvieron como control. Periódicamente se midió la longitud de los brotes, y se muestrearon yemas, y hojas y flores para analizar la calidad de las yemas mediante microscopía óptica y su contenido nutricional.[EN] Currently, after the persimmon harvesting delay promoted by the gibberellic acid and calcium salts, it has been observed the general decay in the vigor that affects negatively in the next sprouting and flowering and therefore, in the harvesting. The goal of this paper is to study if this decline in the vegetative development could normalize with the hormone application and nitrogenous compounds and which is its nutritional behavior. In order to this, it has been done a vegetative development monitoring of 5 buds in each of the 20 trees, being 10 of these trees treated in autumn(30th September, 24th October and 8th November) with GA to delay the harvest. In the sprouting (28th March), it has been applicated in a part of these trees a mixture of GA(2.5g) and nitrogenous compound (28%N), and also to other trees that were kept as a control in autumn, and this process was repeated again 35 days later (2nd May). Seven trees were kept as a total control. Periodically it was measured the sprout length, and it was sampled the buds, leaves and flowers to analyze the buds quality by means of optic microscopy and their nutritional contents.García Vega, LG. (2017). Influencia de las aplicaciones hormonales para el retraso de la recolección en el caqui 'Rojo Brillante' sobre la brotación, desarrollo vegetativo y floración. Técnicas de estímulo. http://hdl.handle.net/10251/88263.TFG

Un caso práctico de meta-evaluación docente: el manual de evaluación de la Universidad Politécnica de Cataluña

La Universidad Politécnica de Cataluña ha sido pionera en la introducción de un modelo de eva- luación docente plenamente adaptado a las exigencias del Espacio Europeo de Enseñanza Supe- rior. Además, su sistema de evaluación de la actividad docente del profesorado ha sido homologa- do por la AQU y ANECA. Dicho modelo de evaluación docente se basa en las más recientes di- rectrices europeas y tiene como fundamento diversos indicadores indirectos. Este modelo se ha em- pleado para evaluar la docencia del profesorado en cinco convocatorias. Durante este periodo de tiempo se ha acumulado una información valiosísima que permite obtener datos agregados no só- lo de la calidad de los docentes sino, también, de diversas características inherentes al proceso de evaluación, así como de la bondad de los indicadores empleados. En este artículo se presentan es- tos datos y se muestra un conjunto de datos agregados que permiten estimar la bondad y fiabilidad del proceso de evaluación, así como su aceptación por el profesorado evaluado.Peer ReviewedPostprint (published version

Phase II study of preoperative bevacizumab, capecitabine and radiotherapy for resectable locally-advanced rectal cancer

Background: to evaluate whether the addition of bevacizumab (BVZ) to capecitabine-based chemoradiotherapy in the preoperative treatment of locally advanced rectal cancer (LARC) improves efficacy measured by the pathological complete response (pCR) rate. Methods: a phase II two-step design was performed. Patients received four cycles of therapy consisting of: BVZ 10 mg/kg in first infusion on day 1 and 5 mg/kg on days 15, 29, 43, capecitabine 1800 mg/m2/day 5 days per week during radiotherapy, which consisted of external-beam irradiation (45 Gy in 1.8 Gy dose per session over 5 sessions/week for 5 weeks). Six to eight weeks after completion of all therapies surgery was undergone. To profile the biological behaviour during BVZ treatment we measured molecular biomarkers before treatment, during BVZ monotherapy, and during and after combination therapy. Microvessel density (MVD) was measured after surgery. Results: forty-three patients were assessed and 41 were included in the study. Three patients achieved a pathological complete response (3/40: 7.5%) and 27 (67.5%) had a pathological partial response, (overall pathological response rate of 75%). A further 8 patients (20%) had stable disease, giving a disease control rate of 95%. Downstaging occurred in 31 (31/40: 77.5%) of the patients evaluated. This treatment resulted in an actuarial 4-year disease-free and overall survival of 85.4 and 92.7% respectively. BVZ with chemoradiotherapy showed acceptable toxicity. No correlations were observed between biomarker results and efficacy variables. Conclusion: BVZ with capecitabine and radiotherapy seem safe and active and produce promising survival results in LARC

Computational tools for splicing defect prediction in breast/ovarian cancer genes: how efficient are they at predicting RNA alterations?

In silico tools for splicing defect prediction have a key role to assess the impact of variants of uncertain significance. Our aim was to evaluate the performance of a set of commonly used splicing in silico tools comparing the predictions against RNA in vitro results. This was done for natural splice sites of clinically relevant genes in hereditary breast/ovarian cancer (HBOC) and Lynch syndrome. A study divided into two stages was used to evaluate SSF-like, MaxEntScan, NNSplice, HSF, SPANR, and dbscSNV tools. A discovery dataset of 99 variants with unequivocal results of RNA in vitro studies, located in the 10 exonic and 20 intronic nucleotides adjacent to exon-intron boundaries of BRCA1, BRCA2, MLH1, MSH2, MSH6, PMS2, ATM, BRIP1, CDH1, PALB2, PTEN, RAD51D, STK11, and TP53, was collected from four Spanish cancer genetic laboratories. The best stand-alone predictors or combinations were validated with a set of 346 variants in the same genes with clear splicing outcomes reported in the literature. Sensitivity, specificity, accuracy, negative predictive value (NPV) and Mathews Coefficient Correlation (MCC) scores were used to measure the performance. The discovery stage showed that HSF and SSF-like were the most accurate for variants at the donor and acceptor region, respectively. The further combination analysis revealed that HSF, HSF+SSF-like or HSF+SSF-like+MES achieved a high performance for predicting the disruption of donor sites, and SSF-like or a sequential combination of MES and SSF-like for predicting disruption of acceptor sites. The performance confirmation of these last results with the validation dataset, indicated that the highest sensitivity, accuracy, and NPV (99.44%, 99.44%, and 96.88, respectively) were attained with HSF+SSF-like or HSF+SSF-like+MES for donor sites and SSF-like (92.63%, 92.65%, and 84.44, respectively) for acceptor sites. We provide recommendations for combining algorithms to conduct in silico splicing analysis that achieved a high performance. The high NPV obtained allows to select the variants in which the study by in vitro RNA analysis is mandatory against those with a negligible probability of being spliceogenic. Our study also shows that the performance of each specific predictor varies depending on whether the natural splicing sites are donors or acceptors

GEODIVULGAR: Geología y Sociedad

Fac. de Ciencias GeológicasFALSEsubmitte

Treatment with tocilizumab or corticosteroids for COVID-19 patients with hyperinflammatory state: a multicentre cohort study (SAM-COVID-19)

Objectives: The objective of this study was to estimate the association between tocilizumab or corticosteroids and the risk of intubation or death in patients with coronavirus disease 19 (COVID-19) with a hyperinflammatory state according to clinical and laboratory parameters. Methods: A cohort study was performed in 60 Spanish hospitals including 778 patients with COVID-19 and clinical and laboratory data indicative of a hyperinflammatory state. Treatment was mainly with tocilizumab, an intermediate-high dose of corticosteroids (IHDC), a pulse dose of corticosteroids (PDC), combination therapy, or no treatment. Primary outcome was intubation or death; follow-up was 21 days. Propensity score-adjusted estimations using Cox regression (logistic regression if needed) were calculated. Propensity scores were used as confounders, matching variables and for the inverse probability of treatment weights (IPTWs). Results: In all, 88, 117, 78 and 151 patients treated with tocilizumab, IHDC, PDC, and combination therapy, respectively, were compared with 344 untreated patients. The primary endpoint occurred in 10 (11.4%), 27 (23.1%), 12 (15.4%), 40 (25.6%) and 69 (21.1%), respectively. The IPTW-based hazard ratios (odds ratio for combination therapy) for the primary endpoint were 0.32 (95%CI 0.22-0.47; p < 0.001) for tocilizumab, 0.82 (0.71-1.30; p 0.82) for IHDC, 0.61 (0.43-0.86; p 0.006) for PDC, and 1.17 (0.86-1.58; p 0.30) for combination therapy. Other applications of the propensity score provided similar results, but were not significant for PDC. Tocilizumab was also associated with lower hazard of death alone in IPTW analysis (0.07; 0.02-0.17; p < 0.001). Conclusions: Tocilizumab might be useful in COVID-19 patients with a hyperinflammatory state and should be prioritized for randomized trials in this situatio

Famílies botàniques de plantes medicinals

Facultat de Farmàcia, Universitat de Barcelona. Ensenyament: Grau de Farmàcia, Assignatura: Botànica Farmacèutica, Curs: 2013-2014, Coordinadors: Joan Simon, Cèsar Blanché i Maria Bosch.Els materials que aquí es presenten són els recull de 175 treballs d’una família botànica d’interès medicinal realitzats de manera individual. Els treballs han estat realitzat per la totalitat dels estudiants dels grups M-2 i M-3 de l’assignatura Botànica Farmacèutica durant els mesos d’abril i maig del curs 2013-14. Tots els treballs s’han dut a terme a través de la plataforma de GoogleDocs i han estat tutoritzats pel professor de l’assignatura i revisats i finalment co-avaluats entre els propis estudiants. L’objectiu principal de l’activitat ha estat fomentar l’aprenentatge autònom i col·laboratiu en Botànica farmacèutica

Large scale multifactorial likelihood quantitative analysis of BRCA1 and BRCA2 variants: An ENIGMA resource to support clinical variant classification

The multifactorial likelihood analysis method has demonstrated utility for quantitative assessment of variant pathogenicity for multiple cancer syndrome genes. Independent data types currently incorporated in the model for assessing BRCA1 and BRCA2 variants include clinically calibrated prior probability of pathogenicity based on variant location and bioinformatic prediction of variant effect, co-segregation, family cancer history profile, co-occurrence with a pathogenic variant in the same gene, breast tumor pathology, and case-control information. Research and clinical data for multifactorial likelihood analysis were collated for 1,395 BRCA1/2 predominantly intronic and missense variants, enabling classification based on posterior probability of pathogenicity for 734 variants: 447 variants were classified as (likely) benign, and 94 as (likely) pathogenic; and 248 classifications were new or considerably altered relative to ClinVar submissions. Classifications were compared with information not yet included in the likelihood model, and evidence strengths aligned to those recommended for ACMG/AMP classification codes. Altered mRNA splicing or function relative to known nonpathogenic variant controls were moderately to strongly predictive of variant pathogenicity. Variant absence in population datasets provided supporting evidence for variant pathogenicity. These findings have direct relevance for BRCA1 and BRCA2 variant evaluation, and justify the need for gene-specific calibration of evidence types used for variant classification

Forzado de emisión de brotes en la papaya

[ES] Se trata de la aplicación de dos diferentes concentraciones de hormonas en las yemas con 3 tratamientos distintos para ver el resultado en los brotes emitidos. Dichas hormonas, se aplican sobre 18 yemas de 24 plantas de papaya, siendo 12 de las plantas tratadas con la concentración C1 (5 mg de BAP + 5 mg de GAs en 10 ml de lanolina) y las otras 12 con la C2 (5 mg de BAP+ 10 mg de GAs en 10 ml de lanolina). Dentro de cada 12 plantas, habrán 4 que corresponden al tratamiento 1 (cubiertas con plástico negro), otras 4 al tratamiento 2 (cubiertas de plástico transparente) y las últimas 4 al tratamiento 3 (sin plástico), todo bajo invernadero. Por tanto, siendo 2 concentraciones y 3 tratamientos, son 6 diferentes combinaciones a las cuales corresponden 4 repeticiones por combinación. Tras realizar un seguimiento, nos damos cuenta de que las diferentes concentraciones no afectan a los resultados, afectando en diferentes aspectos los tratamientos utilizados.[EN] The research developed addresses the application of two concentrations of hormones in the yolks with two different kinds of covered, black and clear plastic, compared without covered. Used like controls, for improving the outbreaks cast for its rooting like propagation technique. These hormones are applied on eighteen yolks of twenty-four papaya plants, being twelve of them treated with 5 BAP mg + 5 mg of GAs in 10 ml of lanoline (C1) and the other 12 by using 5 BAP mg + 10 mg of GAs in 10 ml of lanoline (C2). Was designed an experiment randomized block with 4 repetitions. Was realized a track about the percentage of outbreaking yolks, the outbreaks in each folk, total number of outbreaks, the evolution of the damaged outbreaks while its development, of the survivors and its length. After tracking, we realize that different concentrations do not affect the results, affecting differently each of the treatments used and being the clear plastic covered the best results obtained, although with the black plastic covered, the etiolated outbreaks were longer.García Vega, LG. (2015). Forzado de emisión de brotes en la papaya. http://hdl.handle.net/10251/54215.TFG

Un caso práctico de meta-evaluación docente: el manual de evaluación de la Universidad Politécnica de Cataluña

La Universidad Politécnica de Cataluña ha sido pionera en la introducción de un modelo de eva- luación docente plenamente adaptado a las exigencias del Espacio Europeo de Enseñanza Supe- rior. Además, su sistema de evaluación de la actividad docente del profesorado ha sido homologa- do por la AQU y ANECA. Dicho modelo de evaluación docente se basa en las más recientes di- rectrices europeas y tiene como fundamento diversos indicadores indirectos. Este modelo se ha em- pleado para evaluar la docencia del profesorado en cinco convocatorias. Durante este periodo de tiempo se ha acumulado una información valiosísima que permite obtener datos agregados no só- lo de la calidad de los docentes sino, también, de diversas características inherentes al proceso de evaluación, así como de la bondad de los indicadores empleados. En este artículo se presentan es- tos datos y se muestra un conjunto de datos agregados que permiten estimar la bondad y fiabilidad del proceso de evaluación, así como su aceptación por el profesorado evaluado.Peer Reviewe