AMAZONS FROM MADRID TO VIENNA, BY WAY OF ITALY: THE CIRCULATION OF A SPANISH TEXT AND THE DEFINITION OF AN IMAGINARY

The fascinating phenomenon of the migration of theatrical subjects between literary genres, languages and countries is enriched through a new example discussed in this article. A handwritten libretto compiled in Rome for the court of Vienna, La simpatia nell'odio, overo Le amazoni amanti by Giovanni Pietro Monesio (1664), was discovered to be a faithful translation of the Spanish play Las Amazonas by Antonio de Solís (1657), hitherto known as the basis of a much later libretto, Caduta del regno delle Amazzoni, by Domenico De Totis (1690), set by Bernardo Pasquini. Monesio's libretto not only allows us to reconstruct the manner and time of the European circulation of a Spanish subject (from Madrid to Rome, to Vienna and Naples), but also to shed light on wider cultural aspects. First of all, it increases the number of librettos closely based on Spanish plays, of which so far only a few examples by Giulio Rospigliosi were known. Secondly, it provides further proof of the hypotheticised Spanish influence on the fortune of the Amazons in Italian opera and confirmation that its first appearance was beyond the Alps rather than in Italy. Finally, in the context of the the Habsburg Viennese court, the success of the Amazons seems to be linked to the political need to create a strong symbolic association between the ‘discourse' on the virago and the legitimation of female powe

The Integration of Computers into the Classroom as School Equipment: A Primary School Case Study

AbstractThe past three years have seen a growing tendency on the part of Italian schools to embark on digitalization projects involving the integration of new technologies into the classroom. The current contribution examines the implications of this process, taking three classes at an Italian primary school as a case study. The findings suggest that teachers need to acquire a high level of digital skills in order to implement lessons with significant use of technology, and that the PC may be viewed as an “all-in-one product” although it cannot be expected to substitute all the tools of the traditional classroom

Ifn-g induction by the tc13tul antigen from trypanosoma cruzi in naïve balb/c mice

Trypanosoma cruzi, the etiological agent of Chagas disease, releases factors which modulate the host immune responses, including Tc13 antigens. Regarding innate immune responses, Tc13 antigen from Tulahuén strain, Tc13Tul, has shown to induce B cell expansion and non-specific IgM production on cultures of splenocytes from naïve BALB/c mice. To obtain further information about this role, we evaluated Tc13Tul ability to induce cytokine secretion by in vitro and in vivo stimulation. In vitro Tc13Tul stimulation of splenocytes from naïve BALB/c mice induced higher IFN-g secretion than that induced by the control protein MBP (554 ± 140 pg/ml and 35.33 ± 18.56 pg/ml, respectively). Differences in neither IL-17 nor IL-4 were detected. Tc13Tul-induced IFN-? secretion was also observed in cultured naïve splenocytes from the LPS-resistant C3H/HeJ mouse strain. In vivo administration of Tc13Tul (or MBP as control) to naïve BALB/c mice (3 daily ip doses of 1 ?g/mouse/dose) increased non-specific IgG in sera. In addition, in vitro cultured splenocytes from Tc13Tul-inoculated mice secreted a higher basal level of non-specific IgM than controls and the in vitro Tc13Tul stimulation of these cells showed an additive effect on IgM secretion. Regarding Tc13Tul-induced IFN-g secretion, in vitro cultured splenocytes from Tc13Tul-inoculated mice have no differences in basal levels respect to controls; however, when splenocytes were in vitro stimulated with Tc13Tul, cells from Tc13Tul-inoculated mice showed higher IFN-g secretion than cells from MBP-inoculated mice (950 ± 265.2 pg/ml and 147.3 ± 55.48 pg/ml, respectively). Results indicate that Tc13Tul participation in the innate immune response against T. cruzi is mainly exerted in phenomena related to the evasion of the immune system, such as non-specific Ig production. In contrast, as IFN-g is an important factor involved in T. cruzi resistance, this may be considered a Tc13Tul effect in favor to the host.Fil: Tasso, Laura Mónica. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garavaglia, Patricia Andrea. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Esteva, Mónica Inés. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; ArgentinaFil: Bruballa, Andrea Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Albareda, María Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Gabriela Andrea. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”. Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben”; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaXXXI Reunión de la Sociedad Argentina de ProtozoologíaMar del PlataArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina de Protozoologi

Effect of the Tc13Tul antigen from Trypanosoma cruzi on splenocytes from naïve mice

Trypanosoma cruzi, the etiological agent of Chagas disease, releases factors, including antigens from the trans-sialidase (TS) superfamily, which modulate the host immune responses. Tc13 antigens belong to group IV of TSs and are characterized by C-terminal EPKSA repeats. Here, we studied the effect of the Tc13 antigen from the Tulahuén strain, Tc13Tul, on primary cultures of splenocytes from naïve BALB/c mice. Recombinant Tc13Tul increased the percentage of viable cells and induced B (CD19+) lymphocyte proliferation. Tc13Tul stimulation also induced secretion of non-specific IgM and interferon-γ (IFN-γ). The same effects were induced by Tc13Tul on splenocytes from naïve C3H/HeJ mice. In vivo administration of Tc13Tul to naïve BALB/c mice increased non-specific IgG in sera. In addition, in vitro cultured splenocytes from Tc13Tul-inoculated mice secreted a higher basal level of non-specific IgM than controls and the in vitro Tc13Tul stimulation of these cells showed an enhanced effect on IgM and IFN-γ secretion. Our results indicate that Tc13Tul may participate in the early immunity in T. cruzi infection by favouring immune system evasion through B-cell activation and non-specific Ig secretion. In contrast, as IFN-γ is an important factor involved in T. cruzi resistance, this may be considered a Tc13Tul effect in favour of the host.Fil: Tasso, Laura Mónica. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bruballa, Andrea Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Garavaglia, Patricia Andrea. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; ArgentinaFil: Esteva, Mónica Inés. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; ArgentinaFil: Albareda, María Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Garcia, Gabriela Andrea. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbrán". Instituto Nacional de Parasitología "Dr. Mario Fatala Chaben"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

Lo sguardo degli studenti sulle ingiustizie a scuola

Students’ perceptions regarding injustice at school are widespread in every educational stages and refer especially to classroom management and learning assessment strategies used by teachers. In this contribution we present the results of an investigation about injustice at school, realized asking to 175 university students of three different courses (Primary education sciences and Human Resource Development at University of Milan – Bicocca and Engineering Management of Politecnico di Milano) to describe one incident of injusticeregarding their past school experiences. The research was conducted using mixed method approach. The results highlight the importance of student’s perception of injustice about the relationship between students and teachers and among students, the importance of assessment criteria and the importance of teaching strategies adopted.I vissuti di ingiustizia sono molto diffusi tra gli studenti di qualsiasi ordine di scuola ed essi si riferiscono soprattutto alla gestione della classe da parte degli insegnanti e alla valutazione degli apprendimenti. In questo articolo vengono riportati i primi risultati di un’indagine condotta chiedendo a 175 studenti universitari di Scienze della Formazione Primaria, Formazione e Sviluppo delle Risorse Umane (Università di Milano Bicocca) e di Ingegneria Gestionale (Politecnico di Milano) di descrivere un episodio di ingiustizia di cui erano stati soggetti o testimoni a scuola. L’analisi, condotta con metodi qualitativi e quantitativi, secondo l’approccio mixed-method, ha fornito interessanti riflessioni sul peso che i vissuti di ingiustizia degli studenti hanno nella relazione in classe con gli insegnanti e con i compagni, nella valutazione e più in generale nel processo di insegnamento e apprendimento

Giustizia e ingiustizia a scuola nelle parole dei futuri maestri

The words justice and injustice often feature in students’ reflections on their experience of school, most frequently in relation to assessment, teacher-student relations and modes of class management.In this paper, we present the preliminary results of a study in which 82 trainee primary school teachers described both an episode of justice and an episode of injustice that they had either directly experienced or witnessed during their own school years. The data analysis was carried out using mixed-mode methodology and provided interesting insights into issues such as assessment and relationships in the classroom, as well as highlighting the need for teachers to receive training in interpersonal skills to complement their subject knowledge.Le parole giustizia e ingiustizia ricorrono spesso nelle riflessioni che gli studenti fanno sulla loro esperienza scolastica e coinvolgono la valutazione scolastica, la relazione insegnante-allievi e in generale le modalità con cui è gestita la classe. In questo articolo vengono riportati i primi risultati di un’indagine condotta chiedendo a 82 studenti universitari di Scienze della Formazione Primaria di descrivere un episodio di giustizia e uno di ingiustizia di cui erano stati soggetti o testimoni a scuola. L’analisi, condotta con metodi qualitativi e quantitativi secondo il paradigma del mixed-method, ha fornito interessanti riflessioni sul tema della valutazione e della relazione in classe oltre a suggerire indicazioni per una formazione degli insegnanti che ai contenuti disciplinari affianchi buone capacità relazionali

The dual nature of trehalose in citrus canker disease: A virulence factor for Xanthomonas citri subsp. citri and a trigger for plant defence responses

Xanthomonas citri subsp. citri (Xcc) is a bacterial pathogen that causes citrus canker in susceptible Citrus spp. The Xcc genome contains genes encoding enzymes from three separate pathways of trehalose biosynthesis. Expression of genes encoding trehalose-6-phosphate synthase (otsA) and trehalose phosphatase (otsB) was highly induced during canker development, suggesting that the two-step pathway of trehalose biosynthesis via trehalose-6-phosphate has a function in pathogenesis. This pathway was eliminated from the bacterium by deletion of the otsA gene. The resulting XccΔotsA mutant produced less trehalose than the wild-type strain, was less resistant to salt and oxidative stresses, and was less able to colonize plant tissues. Gene expression and proteomic analyses of infected leaves showed that infection with XccΔotsA triggered only weak defence responses in the plant compared with infection with Xcc, and had less impact on the host plant's metabolism than the wild-type strain. These results suggested that trehalose of bacterial origin, synthesized via the otsA-otsB pathway, in Xcc, plays a role in modifying the host plant's metabolism to its own advantage but is also perceived by the plant as a sign of pathogen attack. Thus, trehalose biosynthesis has both positive and negative consequences for Xcc. On the one hand, it enables this bacterial pathogen to survive in the inhospitable environment of the leaf surface before infection and exploit the host plant's resources after infection, but on the other hand, it is a tell-tale sign of the pathogen's presence that triggers the plant to defend itself against infection.Fil: Piazza, Ainelén Melanie. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Zimaro, Tamara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Garavaglia, Betiana Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Ficarra, Florencia Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Thomas, Ludivine. King Abdullah University of Science and Technology; Arabia SauditaFil: Marondedze, Claudius. King Abdullah University of Science and Technology; Arabia SauditaFil: Feil, Regina. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Lunn, John E.. Max Planck Institute of Molecular Plant Physiology; AlemaniaFil: Gehring, Chris. King Abdullah University of Science and Technology; Arabia SauditaFil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentin

¿Es posible la enseñanza por competencias en disciplinas básicas? : Una experiencia práctica en el curso de Anatomía I de la Facultad de Ciencias Veterinarias de la UNLP

El trabajo en equipo es una competencia de carácter genérico necesaria para completar el perfil de un profesional que se desarrollará en un escenario laboral complejo. Esta competencia se considera fundamental en la constitución de un profesional comprometido, solidario y que desarrollara un mayor potencial en conjunto con otros profesionales y la sociedad. El presente trabajo pretende evaluar si es posible comenzar a desarrollar en alumnos del curso de Anatomía I de la carrera de Ciencias Veterinarias de la UNLP la competencia “trabajo en equipo”. Se planteó como una experiencia extracurricular voluntaria con una estrategia con modalidad presencial y no presencial utilizando como metodología la resolución de problemas. El 48 % de los alumnos que participaron de la experiencia lograron obtener el mayor nivel de logro que era “resolver el problema superando los requerimientos mínimos y mostraron interés en que todo el equipo sea reconocido por la tarea realizada”. A pesar de que el porcentaje es interesante para un curso de primer año de la carrera, no podemos inferir que la enseñanza por competencias se pueda desarrollar como única estrategia de enseñanza.Eje temático 2: Los cambios e innovaciones en los procesos de formación a - Alternativas didácticas y experiencias de renovación de la enseñanzaFacultad de Ciencias Agrarias y Forestale

Clinical and Biochemical Features in a Patient With Mitochondrial Fission Factor Gene Alteration

Mitochondrial Fission Factor (MFF) is part of a protein complex that promotes mitochondria and peroxisome fission. Hitherto, only 5 patients have been reported harboring mutations in MFF, all of them with the clinical features of a very early onset Leigh-like encephalopathy. We report on an 11-year-old boy with epileptic encephalopathy. He presented with neurological regression, epileptic myoclonic seizures, severe intellectual disability, microcephaly, tetraparesis, optic atrophy, and ophthalmoplegia. Brain MRI pattern was compatible with Leigh syndrome. NGS-based analysis of a gene panel for mitochondrial disorders revealed a homozygous c.892C>T (p. Arg298*) in the MFF gene. Fluorescence staining detected abnormal morphology of mitochondria and peroxisomes in fibroblasts from the patient; a strong reduction in MFF protein levels and the presence of truncated forms were observed. No biochemical alterations denoting peroxisomal disorders were found. As reported in other disorders affecting the dynamics of intracellular organelles, our patient showed clinical features suggesting both mitochondrial and peroxisomal impairment. High levels of lactate in our case suggested an involvement of the energetic metabolism but without clear respiratory chain deficiency, while biomarkers of peroxisomal dysfunction were normal. We confirm that MFF mutations are associated with epileptic encephalopathy with Leigh-like MRI pattern