Area preserving analytic flows with dense orbits

The aim of this paper is to give sufficient conditions on area-preserving flows that guarantee the existence of dense orbits. We also answer a question by M.D. Hirsch [M.D. Hirsch, Dense recurrence in area-preserving flows on surfaces, Nonlinearity 12 (1999) 1545–1553]. The results of this work are a generalization of the ones in [M.D. Hirsch, Dense recurrence in area-preserving flows on surfaces, Nonlinearity 12 (1999) 1545–1553] and [H. Marzougui, Area preserving flows with a dense orbit, Nonlinearity 15 (2002) 1379– 1384].The first author was supported by MERST(Ministery of Higher Education,Scientifc Research and Technology,Tunisia)under the Spanish-Tunisian project,grant A/8322/07.The second one was partially supported by MEC(Ministerio de Educación y Ciencia,Spain)and FEDER(Fondo Europeo de Desarrollo Regional),grant MTM2005- 03868,and Fundación Séneca(Comunidad Autónoma de la Región de Murcia,Spain),grant 00684/PI/04

On a Morse conjecture for analytic flows on compact surfaces

The aim of this paper is to prove a Morse conjecture; in particular it is shown that a topologically transitive analytic flow on a compact surface is metrically transitive. We also build smooth topologically transitive flows on surfaces which are not metrically transitive.The first author was supported by the research unit “Systèmes dynamiques et Combinatoire” 99UR/15-15. The second one was partially supported by MEC (Ministerio de Educación y Ciencia, Spain) and FEDER (Fondo Europeo de Desarrollo Regional), grant MTM2005-03868, and Fundación Séneca (Comunidad Autónoma de la Región de Murcia, Spain), grant 00684/PI/04

On the accuracy of the Perturbative Approach for Strong Lensing: Local Distortion for Pseudo-Elliptical Models

The Perturbative Approach (PA) introduced by \citet{alard07} provides analytic solutions for gravitational arcs by solving the lens equation linearized around the Einstein ring solution. This is a powerful method for lens inversion and simulations in that it can be used, in principle, for generic lens models. In this paper we aim to quantify the domain of validity of this method for three quantities derived from the linearized mapping: caustics, critical curves, and the deformation cross section (i.e. the arc cross section in the infinitesimal circular source approximation). We consider lens models with elliptical potentials, in particular the Singular Isothermal Elliptic Potential and Pseudo-Elliptical Navarro--Frenk--White models. We show that the PA is exact for this first model. For the second, we obtain constraints on the model parameter space (given by the potential ellipticity parameter $\varepsilon$ and characteristic convergence $\kappa_s$) such that the PA is accurate for the aforementioned quantities. In this process we obtain analytic expressions for several lensing functions, which are valid for the PA in general. The determination of this domain of validity could have significant implications for the use of the PA, but it still needs to be probed with extended sources.Comment: Accepted for publication in MNRA

Validation of an immunochromatographic assay kit for the identification of the Mycobacterium tuberculosis complex.

International audienceThe performance of the immunochromatographic assay, SD BIOLINE TB Ag MPT64 RAPID®, was evaluated in Madagascar. Using mouse anti-MPT64 monoclonal antibodies for rapid discrimination between the Mycobacterium tuberculosis complex and nontuberculous mycobacteria, the kit was tested on mycobacteria and other pathogens using conventional methods as the gold standard. The results presented here indicate that this kit has excellent sensitivity (100%) and specificity (100%) compared to standard biochemical detection and can be easily used for the rapid identification of M. tuberculosis complex

Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood

As the tissue that contains the largest representation of the human proteome [1], blood is the most important fluid for clinical diagnostics [2, 3, 4]. However, although changes of plasma protein profiles reflect physiological or pathological conditions associated with many human diseases, only a handful of plasma proteins are routinely used in clinical tests. Reasons for this include the intrinsic complexity of the plasma proteome [1], the heterogeneity of human diseases and the rapid degradation of proteins in sampled blood [5]. We report an integrated microfluidic system, the integrated blood barcode chip that can sensitively sample a large panel of protein biomarkers over broad concentration ranges and within 10 min of sample collection. It enables on-chip blood separation and rapid measurement of a panel of plasma proteins from quantities of whole blood as small as those obtained by a finger prick. Our device holds potential for inexpensive, noninvasive and informative clinical diagnoses, particularly in point-of-care settings

El monte pampeano y sus mamíferos

El oeste pampeano y sus mamíferos, una larga historia marcada por transformaciones ambientales. El monte de llanuras y mesetas (de aquí en más monte) es una de las dieciocho regiones naturales o ecorregiones de nuestro país y conforma una diagonal árida que se extiende desde el sur de San Juan hasta la costa atlántica de Chubut, Río Negro y el sur de Buenos Aires. Esta diagonal es a su vez parte de una región mayor, denominada ‘zona de transición sudamericana’, que tiene una larga historia evolutiva y, sin dudas, ha jugado un rol biogeográfico clave para la biota terrestre del sur de Sudamérica...Fil: Soibelzon, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Negrete, Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Habib, Delfino Ahumada. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Montero, Raúl. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Ciai, Dante Nahuel. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo; ArgentinaFil: Martin, Gabriel Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagónica. Universidad Nacional de la Patagonia "San Juan Bosco". Centro de Investigación Esquel de Montaña y Estepa Patagónica; Argentin

A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells

Cellular immunity has an inherent high level of functional heterogeneity. Capturing the full spectrum of these functions requires analysis of large numbers of effector molecules from single cells. We report a microfluidic platform designed for highly multiplexed (more than ten proteins), reliable, sample-efficient (~1 × 10^4 cells) and quantitative measurements of secreted proteins from single cells. We validated the platform by assessment of multiple inflammatory cytokines from lipopolysaccharide (LPS)-stimulated human macrophages and comparison to standard immunotechnologies. We applied the platform toward the ex vivo quantification of T cell polyfunctional diversity via the simultaneous measurement of a dozen effector molecules secreted from tumor antigen–specific cytotoxic T lymphocytes (CTLs) that were actively responding to tumor and compared against a cohort of healthy donor controls. We observed profound, yet focused, functional heterogeneity in active tumor antigen–specific CTLs, with the major functional phenotypes quantitatively identified. The platform represents a new and informative tool for immune monitoring and clinical assessment

Automatic segmentation of multiple cardiovascular structures from cardiac computed tomography angiography images using deep learning.

OBJECTIVES:To develop, demonstrate and evaluate an automated deep learning method for multiple cardiovascular structure segmentation. BACKGROUND:Segmentation of cardiovascular images is resource-intensive. We design an automated deep learning method for the segmentation of multiple structures from Coronary Computed Tomography Angiography (CCTA) images. METHODS:Images from a multicenter registry of patients that underwent clinically-indicated CCTA were used. The proximal ascending and descending aorta (PAA, DA), superior and inferior vena cavae (SVC, IVC), pulmonary artery (PA), coronary sinus (CS), right ventricular wall (RVW) and left atrial wall (LAW) were annotated as ground truth. The U-net-derived deep learning model was trained, validated and tested in a 70:20:10 split. RESULTS:The dataset comprised 206 patients, with 5.130 billion pixels. Mean age was 59.9 ± 9.4 yrs., and was 42.7% female. An overall median Dice score of 0.820 (0.782, 0.843) was achieved. Median Dice scores for PAA, DA, SVC, IVC, PA, CS, RVW and LAW were 0.969 (0.979, 0.988), 0.953 (0.955, 0.983), 0.937 (0.934, 0.965), 0.903 (0.897, 0.948), 0.775 (0.724, 0.925), 0.720 (0.642, 0.809), 0.685 (0.631, 0.761) and 0.625 (0.596, 0.749) respectively. Apart from the CS, there were no significant differences in performance between sexes or age groups. CONCLUSIONS:An automated deep learning model demonstrated segmentation of multiple cardiovascular structures from CCTA images with reasonable overall accuracy when evaluated on a pixel level

Persistence and clearance of Ebola virus RNA from seminal fluid of Ebola virus disease survivors: a longitudinal analysis and modelling study

Background By January, 2016, all known transmission chains of the Ebola virus disease (EVD) outbreak in west Africa had been stopped. However, there is concern about persistence of Ebola virus in the reproductive tract of men who have survived EVD. We aimed to use biostatistical modelling to describe the dynamics of Ebola virus RNA load in seminal fl uid, including clearance parameters. Methods In this longitudinal study, we recruited men who had been discharged from three Ebola treatment units in Guinea between January and July, 2015. Participants provided samples of seminal fl uid at follow-up every 3–6 weeks, which we tested for Ebola virus RNA using quantitative real-time RT-PCR. Representative specimens from eight participants were then inoculated into immunodefi cient mice to test for infectivity. We used a linear mixed-eff ect model to analyse the dynamics of virus persistence in seminal fl uid over time. Findings We enrolled 26 participants and tested 130 seminal fl uid specimens; median follow up was 197 days (IQR 187–209 days) after enrolment, which corresponded to 255 days (228–287) after disease onset. Ebola virus RNA was detected in 86 semen specimens from 19 (73%) participants. Median duration of Ebola virus RNA detection was 158 days after onset (73–181; maximum 407 days at end of follow-up). Mathematical modelling of the quantitative time-series data showed a mean clearance rate of Ebola virus RNA from seminal fl uid of –0·58 log units per month, although the clearance kinetic varied greatly between participants. Using our biostatistical model, we predict that 50% and 90% of male survivors clear Ebola virus RNA from seminal fl uid at 115 days (90% prediction interval 72–160) and 294 days (212–399) after disease onset, respectively. We also predicted that the number of men positive for Ebola virus RNA in aff ected countries would decrease from about 50 in January 2016, to fewer than 1 person by July, 2016. Infectious virus was detected in 15 of 26 (58%) specimens tested in mice. Interpretation Time to clearance of Ebola virus RNA from seminal fl uid varies greatly between individuals and could be more than 13 months. Our predictions will assist in decision-making about surveillance and preventive measures in EVD outbreaks