Genetics evaluation of phosphorus utilization in tropical cowpea (Vigna unguiculata (L) Walp)

Genetics evaluation of phosphorus utilization in cowpea (Vigna unguiculata (L) Walp) was studied in a cross involving a tropically adapted genotype (IT90K-277-2) with a reported positive response to rock phosphate (RP) and a second genotype (IT89KD-288) that responds negatively to RP, using generation mean analysis of the parents, their F1, F2 and the two backcross generations. Highly significant differences among the six generations with respect to seed phosphorus (P) concentration and grain yield per plant were observed. The F1 progenies produced seeds with higher seed P concentration (0.31 mg/g) than the mid-parent value (0.27mg/g). Mean yield per plant in the F1(48.03g) that was significantly greater than the mid-parent value (28.85g) was also observed suggesting positive heterosis for the two traits. Backcrossing to both parents showed good convergence of genes on the recurrent parents. Possibility of transgressive segregation for seed P concentration and grain yield per plant in the F2 generation was also observed. Absence of significant reciprocal differences among individuals in the F1 and F2 populations suggested lack of maternal and cytoplasmic effects. Non-additive gene action (dominance and epistasis) made significant contributions to total genetic variations in both traits within the cross. Whereas seed P concentration was observed to be under polygenic control, number of genes for seed yield per plant was biased downward by epistasis. Observed narrow-sense heritability estimate (HN)) for seed P concentration of 50.51% and very low (0.040) expected genetic gain in the F3 over the F2 means suggested that although expected progress from selection could be lowered by non-additive gene effects, this trait could be transmitted to the offsprings of hybridization program with huge success, through backcrossing. Although, available soil P (6.03 – 8.09mg/kg) was observed to be below the critical level, phosphorus uptake in the F1 and the segregating generations was observed to be higher than what obtained in the parents to suggest that the progenies of a P-efficient x P-inefficient cross would be efficient in P utilization.Keywords: Generation mean analysis, Phosphorus uptake, Polygenes, Transgressive segregants, Tropical vigna

Impact of soil fertility management practices on the nutritional quality of Soybean (Glycine max (l.) Merr.) varieties grown in Eastern Zambia

Open Access Journal; Published online: 26 Sept 2019Zambia has three primary agro-ecological regions, with each region having specific ecological characteristics. Region II agro-ecological zone of Zambia has low nutrient reserves and poor water holding capacity due to moderately leached clayey to loamy soil; this has led to low soybean productivity. Soil fertility management (ISFM) strategies such as the use of inorganic fertilizers and the use of inoculants of rhizobia have been introduced and promoted among small-scale farmers in Eastern Province. Two soybean varieties (Lukanga and Kafue) were used for this study and 96 samples from on-farm soil fertility management trials in Chipata, and Petauke districts were collected for the determinations of nutritional and anti-nutritional properties. The proximate analysis of Chipata samples showed that the ash content 5.10‒6.23%, fat content 17.71‒25.57%, protein content 27.73‒37.11%, amylose content 1.26‒4.56 %, sugar content 6.75‒9.62%, and starch content 4.00‒18.57%, while anti-nutritional properties ranged between 3.07 and 8.21% for phytate and 1.42‒3.35% for tannin. With Petauke, the ash content 3.32‒6.8 %, fat content 19.16‒26.85%, protein content 27.68‒35.62%, amylose 2.00‒4.37%, sugar content 6.23‒9.76%, and starch content 5.70‒18.63%. Phytate and tannin contents were 3.37‒7.90% and 0.14‒3.32%, respectively. The highest protein content was found at the level of 37.11% in Kafue with 40kg P/ha and inoculant, and the least was 27.73% for Lukanga without inputs in Chipata while in Petauke, Lukanga with 40 P/ha and inoculant had the highest protein content of 35.62% and the least was 27.68% for Lukanga with inoculant. The co-application of rhizobia inoculant and P nutrient increased phytate, and tannin content significantly (P < 0.05)

Trends in mortality by labour market position around retirement ages in three European countries with different welfare regimes

&lt;p&gt;Objectives: In the face of economic downturn and increasing life expectancy, many industrial nations are adopting a policy of postponing the retirement age. However, questions still remain around the consequence of working longer into old age. We examine mortality by work status around retirement ages in countries with different welfare regimes; Finland (social democratic), Turin (Italy; conservative), and England and Wales (liberal).&lt;/p&gt; &lt;p&gt;Methods: Death rates and rate ratios (RRs) (reference rates = ‘in-work’), 1970 s–2000 s, were estimated for those aged 45–64 years using the England and Wales longitudinal study, Turin longitudinal study, and the Finnish linked register study.&lt;/p&gt; &lt;p&gt;Results: Mortality of the not-in-work was consistently higher than the in-work. Death rates for the not-in-work were lowest in Turin and highest in Finland. Rate ratios were smallest in Turin (RR men 1972–76 1.73; 2002–06 1.63; women 1.22; 1.68) and largest in Finland (RR men 1991–95 3.03; 2001–05 3.80; women 3.62; 4.11). Unlike RRs for men, RRs for women increased in every country (greatest in Finland).&lt;/p&gt; &lt;p&gt;Conclusions: These findings signal that overall, employment in later life is associated with lower mortality, regardless of welfare regime.&lt;/p&gt

Informing investment to reduce inequalities: a modelling approach

Background: Reducing health inequalities is an important policy objective but there is limited quantitative information about the impact of specific interventions. Objectives: To provide estimates of the impact of a range of interventions on health and health inequalities. Materials and methods: Literature reviews were conducted to identify the best evidence linking interventions to mortality and hospital admissions. We examined interventions across the determinants of health: a ‘living wage’; changes to benefits, taxation and employment; active travel; tobacco taxation; smoking cessation, alcohol brief interventions, and weight management services. A model was developed to estimate mortality and years of life lost (YLL) in intervention and comparison populations over a 20-year time period following interventions delivered only in the first year. We estimated changes in inequalities using the relative index of inequality (RII). Results: Introduction of a ‘living wage’ generated the largest beneficial health impact, with modest reductions in health inequalities. Benefits increases had modest positive impacts on health and health inequalities. Income tax increases had negative impacts on population health but reduced inequalities, while council tax increases worsened both health and health inequalities. Active travel increases had minimally positive effects on population health but widened health inequalities. Increases in employment reduced inequalities only when targeted to the most deprived groups. Tobacco taxation had modestly positive impacts on health but little impact on health inequalities. Alcohol brief interventions had modestly positive impacts on health and health inequalities only when strongly socially targeted, while smoking cessation and weight-reduction programmes had minimal impacts on health and health inequalities even when socially targeted. Conclusions: Interventions have markedly different effects on mortality, hospitalisations and inequalities. The most effective (and likely cost-effective) interventions for reducing inequalities were regulatory and tax options. Interventions focused on individual agency were much less likely to impact on inequalities, even when targeted at the most deprived communities

T. cruzi OligoC-TesT: A Simplified and Standardized Polymerase Chain Reaction Format for Diagnosis of Chagas Disease

Chagas disease (American trypanosomiasis) is caused by the protozoan parasite Trypanosoma cruzi and represents a major public health problem in Latin America. Furthermore, growing human population movements extend the disease distribution to regions outside the South American continent. Accurate diagnosis is crucial in patient care and in preventing transmission through blood transfusion, organ transplantation, or vertical transmission from mother to child. Routine diagnosis of Trypanosoma cruzi infection generally is based on detection of the host's antibodies against the parasite. However, antibody detection tests are liable to specificity problems and are of limited use in assessing treatment outcome and congenital infections. The introduction of the polymerase chain reaction (PCR) to amplify specific DNA sequences opened promising diagnostic perspectives. Despite its reported high sensitivity and specificity, broad use of the PCR technique in diagnosis of Chagas disease is hampered by its complexity and the lack of any standardization. We here present the development and evaluation of the T. cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA. The new tool is an important step towards simplified and standardized molecular diagnosis of Chagas disease

A protease-based biosensor for the detection of schistosome cercariae

Parasitic diseases affect millions of people worldwide, causing debilitating illnesses and death. Rapid and cost-effective approaches to detect parasites are needed, especially in resource-limited settings. A common signature of parasitic diseases is the release of specific proteases by the parasites at multiple stages during their life cycles. To this end, we engineered several modular Escherichia coli and Bacillus subtilis whole-cell-based biosensors which incorporate an interchangeable protease recognition motif into their designs. Herein, we describe how several of our engineered biosensors have been applied to detect the presence and activity of elastase, an enzyme released by the cercarial larvae stage of Schistosoma mansoni. Collectively, S. mansoni and several other schistosomes are responsible for the infection of an estimated 200 million people worldwide. Since our biosensors are maintained in lyophilised cells, they could be applied for the detection of S. mansoni and other parasites in settings without reliable cold chain access