Synthesis, Conformation and Antiproliferative Activity of Isothiazoloisoxazole 1,1-dioxides

Sixteen new isothiazoloisoxazole 1,1-dioxides, one new isothiazolotriazole and one new isothiazolopyrazole have been synthesised by using 1,3-dipolar cycloadditions to isothiazole 1,1-dioxides. One sub-set of these isothiazoloisoxazoles showed low μM activity against a human breast carcinoma cell line, whilst a second sub-set plus the isothiazolotriazole demonstrated an interesting restricted rotation of sterically hindered bridgehead substituents. A thiazete 1,1-dioxide produced from one of the isothiazole 1,1-dioxides underwent conversion into an unknown 1,2,3-oxathiazolin-2-oxide upon treatment with Lewis acids, but was inert towards 1,3-dipoles and cyclopropenones. Six supporting crystal structures are included

Predictive Values of Transcutaneous Oxygen Tension for Above-the-ankle Amputation in Diabetic Patients with Critical Limb Ischemia

ObjectiveTo assess the values of transcutaneous oxygen tension (TcPO2) capable of predicting above-the-ankle amputation in diabetic patients diagnosed for critical limb ischemia (CLI) according to the criteria of the TransAtlantic Inter-Society Consensus.DesignRetrospective study.MethodsFrom January 1999 to December 2003, 564 diabetic patients were consecutively hospitalized for CLI in one limb. Revascularization with angioplasty or bypass graft was performed when possible and, if not possible, prostanoid therapy was used. In patients in whom therapies did not relieve the rest pain or the gangrene was extended above the Chopart joint, an above-the-ankle-amputation was performed. After treatment TcPO2 values were evaluated in all patients at the dorsum of the foot.ResultsFifty-five (9.8%) patients underwent an above-the-ankle amputation: 22 of 420 patients who underwent angioplasty, 17 of 117 patients who underwent bypass (14.5%) and 16 of 27 patients in whom revascularization was not possible. Post-treatment TcPO2, measured by a receiver operating characteristic (ROC) curve, showed a value 34mmHg as the best threshold for determining the need for revascularization, with an area under the curve of 0.89 (95%CI 0.85–0.94).Using logistic regression analysis the probability of above-the-ankle amputation for this threshold is 9.7% and reduces to 3% for TcPO2>40mmHg.ConclusionTcPO2 levels<34mmHg indicate the need for revascularization, while for values ≥ 34<40mmHg this need appears less pressing, although there remains a considerable probability of amputation. TcPO2 levels greater than 40mmHg suggest that revascularization is dependent on the severity of tissue loss and possible morbidity caused by the procedure

The geometrical nature of optical resonances : from a sphere to fused dimer nanoparticles

We study the electromagnetic response of smooth gold nanoparticles with shapes varying from a single sphere to two ellipsoids joined smoothly at their vertices. We show that the plasmonic resonance visible in the extinction and absorption cross sections shifts to longer wavelengths and eventually disappears as the mid-plane waist of the composite particle becomes narrower. This process corresponds to an increase of the numbers of internal and scattering modes that are mainly confined to the surface and coupled to the incident field. These modes strongly affect the near field, and therefore are of great importance in surface spectroscopy, but are almost undetectable in the far field

Year-round multi-scale habitat selection by Crested Tit (Lophophanes cristatus) in lowland mixed forests (northern Italy)

Determining how animals respond to resource availability across spatial and temporal extents is crucial to understand ecological processes underpinning habitat selection. Here, we used a multi-scale approach to study the year-round habitat selection of the Crested Tit (Lophophanes cristatus) in a semi-natural lowland woodland of northern Italy, analysing different habitat features at each scale. We performed Crested Tit censuses at three different spatial scales. At the macrohabitat scale, we used geolocalized observations of individuals to compute Manly's habitat selection index, based on a detailed land-use map of the study area. At the microhabitat scale, the trees features were compared between presence and absence locations. At the foraging habitat scale, individual foraging birds and their specific position on trees were recorded using focal animal sampling. Censuses were performed during both the breeding (March to May) and wintering (December to January) seasons. At the macrohabitat scale, the Crested Tits significantly selected pure and mixed pine forests and avoided woods of alien plant species, farmlands and urban areas. At the microhabitat scale, old pine woods with dense cover were selected, with no significant difference in the features of tree selection between the two phenological phases. At the foraging habitat scale, the species was observed spending more time foraging in the canopies than in the understorey, using mostly the portion of Scots Pine (Pinus sylvestris) canopies closer to the trunk in winter, while during the breeding period, the whole canopy was visited. Overall, breeding and wintering habitats largely overlapped in the Crested Tit. Based on our findings, lowland Crested Tits can be well defined as true habitat specialists: they are strictly related to some specific coniferous woodland features. Noteworthily, compared to other tit species, which normally show generalist habits during winter, the Crested Tit behaves as a habitat specialist also out of the breeding season. Our study stressed the importance of considering multi-scale (both spatial and phenological) habitat selection in birds

Are the Male Body Dissatisfaction and Drive for Muscularity scales reliable and valid instruments?

The Drive for Muscularity Scale and Male Body Dissatisfaction Scale were developed for use with men and correspond to measures of drive for thinness and body dissatisfaction in women. The psychometric properties of these measures were evaluated in a sample of 655 Italian men, who completed other 11 measures also. Both scales demonstrated excellent internal consistency and temporal stability as well as criterion-related and concurrent validity. Both measures distinguished between men with high and low levels of disordered eating. Confirmatory factor analysis replicated the unidimensional factor structure of both scales. Directions for future research are discussed

Wideband THz time domain spectroscopy based on optical rectification and electro-optic sampling

We present an analytical model describing the full electromagnetic propagation in a THz time-domain spectroscopy (THz-TDS) system, from the THz pulses via Optical Rectification to the detection via Electro Optic-Sampling. While several investigations deal singularly with the many elements that constitute a THz-TDS, in our work we pay particular attention to the modelling of the time-frequency behaviour of all the stages which compose the experimental set-up. Therefore, our model considers the following main aspects: (i) pump beam focusing into the generation crystal; (ii) phase-matching inside both the generation and detection crystals; (iii) chromatic dispersion and absorption inside the crystals; (iv) Fabry-Perot effect; (v) diffraction outside, i.e. along the propagation, (vi) focalization and overlapping between THz and probe beams, (vii) electro-optic sampling. In order to validate our model, we report on the comparison between the simulations and the experimental data obtained from the same set-up, showing their good agreement