Green synthesis of silver nanoparticles using Fagopyrum esculentum starch: antifungal, antibacterial activity and its cytotoxicity

52-63Silver nanoparticles (AgNPs) have been synthesized using Fagopyrum esculentum starch as a stabilizing and reducing agent. This reaction was carried out in an autoclave at 15 psi, 121°C for 20 min. UV-visible spectrum of the colloidal nanoparticles showed the surface plasmon absorption band with maximum absorbance at 418 nm. Interaction between functional groups present in the starch and nanoparticles were analyzed by Fourier-transform infrared spectroscopy (FTIR). Size of the synthesized nanoparticles was found to be in the range of 20-30 nm, as revealed from transmission electron microscopy (TEM). The X-ray diffraction analysis revealed the face-centred cubic (fcc) geometry of silver nanoparticles. The nanoparticles were found to be good antifungal agents against Aspergillus niger. The antibacterial activity of the nanoparticles was also studied. The nanoparticles showed higher inhibitory activity against Gram-negative bacteria (Escherichia coli) than the Gram-positive bacteria (Staphylococcus aureus). These results thus show that F. esculentum starch stabilized AgNPs could be used as a promising antimicrobial agent against bacteria the fungi In vitro cytotoxicity assessment of starch stabilized AgNPs has shown no significant cytotoxic effect on human cervical carcinoma cells lines (HeLa) by MTT assay and AgNPs concentration at 200 ug/ml of showed 86% cell viability

Traumatic Retrobulbar Haemorrhage: Aetio-pathology and management

Retrobulbar haematoma following blunt orbital trauma is a rare,but potentially serious complication, since it can evolve rapidly from visual impairment to permanent loss of vision. This sight-threatening situation most commonly arises from orbital bleeding accompanying undisplaced fractures of the orbital walls, an event that increases the pressure inside theorbit and results in vascular damage to the optic nerve. The clinical presentation includes pain, exophthalmos with proptosis, and internal ophthalmoplegia, with impairment or loss of the pupillary reXex. A thin-layer orbital CT scan is an essential diagnostic aid. Therapy is based on orbitaldecompression, via different surgical approaches, with the intention of reducing the pressure on the nerve and vascular structures inside the orbit. Emergent management is of utmost importance as any delay between the onset of symptoms and treatment can have a significant effect onrecovery

Computational Intelligence In CAD/CAM Applications

This paper presents a fundamental, direct, and powerful approach to the surface/surface intersection problem in CAD/CAM applications. The algorithm is designed and implemented in three steps: a) Preprocessing- locate the potentially intersecting sections of the surfaces and decompose the surfaces into surface elements within specified flatness tolerance; b) Intersection- decompose the possibly intersecting pairs of surface elements into continuous surface triangulations to find the approximate intersections between the pairs of surface elements; c) Postprocessing-assemble the intersection primitives into curves of intersection, refine the accuracy of computed intersection points, and compact the intersection curves. This surface/surface intersection algorithm is applicable to the widest class, C°, of parametric surfaces, an enhancement over the existing algorithms applicable to only Ck, k≥ 1, surfaces. This implementation, based on computational intelligence, requires no human interaction for intersection curve pattern recognition

Quench-induced spontaneous currents in rings of ultracold fermionic atoms

We have measured the rate of spontaneous current formation in ring-shaped ensembles of fermionic $^6$Li atoms, following a thermal quench through the BCS superfluid phase transition. For the fastest quenches, the mean square winding number follows a scaling law with exponent $\sigma$ = 0.24(2), in line with predictions of the Kibble-Zurek (KZ) model for mean-field BCS theory. We use a hybrid quench protocol involving simultaneous evaporation and interaction ramps, with a long system lifetime allowing characterization of a different rate of spontaneous current formation in the slow-quench regime, where finite-size effects are important. Comparing our observations to a quasi-1D stochastic Ginzburg-Landau model, we find quantitative agreement for fast quenches, but only qualitative agreement for slow quenches.Comment: 6 pages, 4 figure

Low temperature co-fired ceramic (LTCC)- based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix

Studies on enzyme based thermometric biosensor are limited. Here, we report on fabrication of an alkaline phosphate based thermometric biosensor. We designed alkaline phosphatase inhibition based biosensor for detection of vanadium using immobilized alkaline phosphatase on multi walled carbon nano tubes (MWCTs) ethyl cellulose sponge matrix. We isolated protein from plant source, partially purified and fabricated a miniature ceramic viz. LTCC (low temperature co-fired ceramics technology) based biosensor for detection of vanadium. This biosensor consists of a microreaction chamber with buried heaters. Alkaline phosphatase has been isolated from the seeds of ‘Arachis hypoghaea’ was studied for its biochemical properties viz. optimum pH and temperature. The partially purified enzyme was immobilized using carboxyl-functionalised carbon nanotubes (CNTs) by cross linking with epichlorohydrin (ECH) along with a matrix of ethyl cellulose. The developed LTCC based biosensor on testing indicated its linear response to vanadium concentration up to 9 mM with a relatively high sensitivity of about 147 nA/mM. Thus, we have demonstrated a LTCC based biosensor using immobilized alkaline phosphatase for detection of vanadium

Low temperature co-fired ceramic (LTCC)based biosensor for detection of vanadium using immobilized Arachis hypogaea alkaline phosphatase on multi walled carbon nanotubes ethyl cellulose sponge matrix

916-920Studies on enzyme based thermometric biosensor are limited. Here, we report on fabrication of an alkaline phosphate based thermometric biosensor. We designed alkaline phosphatase inhibition based biosensor for detection of vanadium using immobilized alkaline phosphatase on multi walled carbon nano tubes (MWCTs) ethyl cellulose sponge matrix. We isolated protein from plant source, partially purified and fabricated a miniature ceramic viz. LTCC (low temperature co-fired ceramics technology) based biosensor for detection of vanadium. This biosensor consists of a microreaction chamber with buried heaters. Alkaline phosphatase has been isolated from the seeds of ‘Arachis hypoghaea’ was studied for its biochemical properties viz. optimum pH and temperature. The partially purified enzyme was immobilized using carboxyl-functionalised carbon nanotubes (CNTs) by cross linking with epichlorohydrin (ECH) along with a matrix of ethyl cellulose. The developed LTCC based biosensor on testing indicated its linear response to vanadium concentration up to 9 mM with a relatively high sensitivity of about 147 nA/mM. Thus, we have demonstrated a LTCC based biosensor using immobilized alkaline phosphatase for detection of vanadium

Growth rate after limb deformity correction by the Ilizarov method with or without knee joint distraction: Lengthening in 30 children followed for at least 2 years

Background and purpose Growth inhibition and stimulation have both been reported after juvenile limb lengthening. Distraction of a joint usually suspends and unloads the growth plate and may stimulate growth. We investigated the influence of knee joint distraction on the speed of growth after limb lengthening

Numerical loop quantum cosmology: an overview

A brief review of various numerical techniques used in loop quantum cosmology and results is presented. These include the way extensive numerical simulations shed insights on the resolution of classical singularities, resulting in the key prediction of the bounce at the Planck scale in different models, and the numerical methods used to analyze the properties of the quantum difference operator and the von Neumann stability issues. Using the quantization of a massless scalar field in an isotropic spacetime as a template, an attempt is made to highlight the complementarity of different methods to gain understanding of the new physics emerging from the quantum theory. Open directions which need to be explored with more refined numerical methods are discussed.Comment: 33 Pages, 4 figures. Invited contribution to appear in Classical and Quantum Gravity special issue on Non-Astrophysical Numerical Relativit