Estudio de la trabeculación ósea en la artrodesis subastragalina

En este artículo presentamos nuestra casuística en artrodesis artroscópica subastragalina revisando los principios biomecánicos que rigen esta articulación y presentando ésta como una técnica válida para el tratamiento de distintas patologías en el retropié que interesan esta articulación.We present our casuistic on arthroscopic subtalar arthrodesis reviewing the biomechanics of this joint and presenting it as a valid technique for the treatment of some pathologies in the rearfoot that affect this joint.Premio al mejor trabajo en el XXXII Congreso de la Sociedad Ibérica de Biomecánica y BiomaterialesPeer ReviewedAward-winnin

Development of the trajectory planner and control system of a spherical robot manipulator embedded in a FPGA board

Best poster presentation Award3rd Doctoral Congress in Engineering will be held at FEUP on the 27th to 28th of June, 2019This work describes the development and implementation of a linear trajectory controller in a five degree of freedom (DOF) robot manipulator (Filho and Quintero 2013). An open loop control architecture was designed and embedded in a Field Programable Gate Array (FPGA), for the first threeDOF. In this paper, we present the direct and inverse kinematics and the Jacobian of the manipulator, used to extract the control equations of the system (Motta, Llanos-Quintero, and Coral Sampaio 2016). This model aims the trajectory planning in a rectilinear path, described in Cartesian Coordinates. For the control implementation embedded in the FPGA, was used the NIOS II microprocessor. This one is responsible for the path planning and for the speed control of the manipulator joints, with some accelerated functions in hardware. The validation of the equations and path planning were done using software simulation. The final structure and experiments of the manipulator are also presented.info:eu-repo/semantics/publishedVersio

Merging BioActuation and BioCapacitive properties: A 3D bioprinted devices to self-stimulate using self-stored energy

Biofabrication of three-dimensional (3D) cultures through the 3D Bioprinting technique opens new perspectives and applications of cell-laden hydrogels. However, to continue with the progress, new BioInks with specific properties must be carefully designed. In this study, we report the synthesis and 3D Bioprinting of an electroconductive BioInk made of gelatin/fibrinogen hydrogel, C2C12 mouse myoblast and 5% w/w of conductive poly (3,4-ethylenedioxythiophene) nanoparticles (PEDOT NPs). The influence of PEDOT NPs, incorporated in the cellladen BioInk, not only showed a positive effect in cells viability, differentiation and myotube functionalities, also allowed the printed constructs to behaved as BioCapacitors. Such devices were able to electrochemically store a significant amount of energy (0.5 mF/cm2), enough to self-stimulate as BioActuator, with typical contractions ranging from 27 to 38 mu N, during nearly 50 min. The biofabrication of 3D constructs with the proposed electroconductive BioInk could lead to new devices for tissue engineering, biohybrid robotics or bioelectronics

MicroRNA Profiling and Bioinformatics Target Analysis in Dorsal Hippocampus of Chronically Stressed Rats: Relevance to Depression Pathophysiology

Indexación: Scopus.1Laboratory of Neuroplasticity and Neurogenetics, Faculty of Chemical and Pharmaceutical Sciences, Department of Biochemistry and Molecular Biology, Universidad de Chile, Santiago, Chile, 2National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Durham, NC, United States, 3Centro de Genómica y Bioinformática, Facultad de Ciencias, Universidad Mayor, Santiago, Chile, 4Millennium Institute for Integrative Biology (iBio), FONDAP Center for Genome Regulation, Departamento de Genética Molecular y Microbiología, Pontificia Universidad Católica de Chile, Santiago, Chile, 5Department of Kinesiology, Faculty of Health Sciences, Universidad Católica del Maule, Talca, Chile, 6Escuela de Química y Farmacia, Facultad de Medicina, Universidad Andres Bello, Santiago, Chile.This study was supported by the following grants: FONDECYT 1120528 (JLF), Fondo Central de Investigación, Universidad de Chile ENL025/16 (JLF), ES090079 (JAC). Research in RG and EV laboratories is funded by Instituto Milenio iBio – Iniciativa Científica Milenio MINECON.Studies conducted in rodents subjected to chronic stress and some observations in humans after psychosocial stress, have allowed to establish a link between stress and the susceptibility to many complex diseases, including mood disorders. The studies in rodents have revealed that chronic exposure to stress negatively affects synaptic plasticity by triggering changes in the production of trophic factors, subunit levels of glutamate ionotropic receptors, neuron morphology, and neurogenesis in the adult hippocampus. These modifications may account for the impairment in learning and memory processes observed in chronically stressed animals. It is plausible then, that stress modifies the interplay between signal transduction cascades and gene expression regulation in the hippocampus, therefore leading to altered neuroplasticity and functioning of neural circuits. Considering that miRNAs play an important role in post-transcriptional-regulation of gene expression and participate in several hippocampus-dependent functions; we evaluated the consequences of chronic stress on the expression of miRNAs in dorsal (anterior) portion of the hippocampus, which participates in memory formation in rodents. Here, we show that male rats exposed to daily restraint stress (2.5 h/day) during 7 and 14 days display a differential profile of miRNA levels in dorsal hippocampus and remarkably, we found that some of these miRNAs belong to the miR-379-410 cluster. We confirmed a rise in miR-92a and miR-485 levels after 14 days of stress by qPCR, an effect that was not mimicked by chronic administration of corticosterone (14 days). Our in silico study identified the top-10 biological functions influenced by miR-92a, nine of which were shared with miR-485: Nervous system development and function, Tissue development, Behavior, Embryonic development, Organ development, Organismal development, Organismal survival, Tissue morphology, and Organ morphology. Furthermore, our in silico study provided a landscape of potential miRNA-92a and miR-485 targets, along with relevant canonical pathways related to axonal guidance signaling and cAMP signaling, which may influence the functioning of several neuroplastic substrates in dorsal hippocampus. Additionally, the combined effect of miR-92a and miR-485 on transcription factors, along with histone-modifying enzymes, may have a functional relevance by producing changes in gene regulatory networks that modify the neuroplastic capacity of the adult dorsal hippocampus under stress. © 2018 Muñoz-Llanos, García-Pérez, Xu, Tejos-Bravo, Vidal, Moyano, Gutiérrez, Aguayo, Pacheco, García-Rojo, Aliaga, Rojas, Cidlowski and Fiedler.https://www.frontiersin.org/articles/10.3389/fnmol.2018.00251/ful

Control of mucocutaneous leishmaniasis, a neglected disease: results of a control programme in Satipo Province, Peru.

Mucocutaneous leishmaniasis (MCL) is an important health problem in many rural areas of Latin America, but there are few data on the results of programmatic approaches to control the disease. We report the results of a control programme in San Martin de Pangoa District, which reports one of the highest prevalences of MCL in Peru. For 2 years (2001--2002), the technicians at the health post were trained in patient case management, received medical support and were supplied with antimonials. An evaluation after 2 years showed the following main achievements: better diagnosis of patients, who were confirmed by microscopy in 34% (82/240) of the cases in 2001 and 60% of the cases (153/254) in 2002; improved follow-up during treatment: 237 of 263 (90%) patients who initiated an antimonial therapy ended the full treatment course; improved follow-up after treatment: 143 of 237 (60%) patients who ended their full treatment were correctly monitored during the required period of 6 (cutaneous cases) or 12 (mucosal cases) months after the end of treatment. These achievements were largely due to the human and logistical resources made available, the constant availability of medications and the close collaboration between the Ministry of Health, a national research institute and an international non-governmental organization. At the end of this period, the health authorities decided to register a generic brand of sodium stibogluconate, which is now in use. This should allow the treatment of a significant number of additional patients, while saving money to invest in other facets of the case management

Degradation of neonicotinoids and caffeine from surface water by photolysis

Funding Information: Publicly available datasets were analyzed in this study. This data can be obtained by contacting the corresponding author of the work at: [email protected] Acknowledgments: Financial support from Fundação para a Ciência e a Tecnologia through the project PTDC/EAM-AMB/30989/2017 is gratefully acknowledged. iNOVA4Health— UID/Multi/04462/2013, a program financially supported by Fundação para a Ciência e Tecnolo-gia/Ministério da Educação e Ciência through national funds and co-funded by FEDER under the PT2020 Partnership Agreement is gratefully acknowledged. This work is also supported by the Associate Laboratory for Green Chemistry—LAQV, which is financed by national funds from FCT/MCTES (UIDB/50006/2020). Funding from the INTERFACE Programme, through the Innovation, Technology and Circular Economy Fund (FITEC), is gratefully acknowledged.Along with rapid social development, the use of insecticides and caffeine-containing products increases, a trend that is also reflected in the composition of surface waters. This study is focused on the phototreatment of a surface water containing three neonicotinoids (imidacloprid, thi-amethoxam, and clothianidin) and caffeine. Firstly, the radiation absorption of the target pollutants and the effect of the water matrix components were evaluated. It was observed that the maximum absorption peaks appear at wavelengths ranging from 246 to 274 nm, and that the water matrix did not affect the efficiency of the removal of the target pollutants. It was found that the insecticides were efficiently removed after a very short exposure to UV irradiation, while the addition of hydrogen peroxide was needed for an efficient caffeine depletion. The electrical energy per order was estimated, being the lowest energy required (9.5 kWh m−3 order−1) for the depletion of thiamethoxan by indirect photolysis, and a concentration of hydrogen peroxide of 5 mg dm−3. Finally, a prelimi-nary evaluation on the formation of by-products reveals that these compounds play a key role in the evolution of the ecotoxicity of the samples, and that the application of direct photolysis reduces the concentration of these intermediates.publishersversionpublishe

Influence of temperament on performance and carcass quality of commercial Brahman steers in a Colombian tropical grazing system

Temperament is defined as individual behavioral responses to potentially fear-eliciting or challenging situations related to human presence and handling. A total of 190 steers of commercial Zebu Brahman (Bos indicus) were used in this study, selected when they were between 10 and 11 months of age, fattened for 24 months (720 days) and slaughtered between 34 and 35 months of age. Using a temperament index (based on two tests: chute and exit score), animals were classified as calm, restless, or nervous. In general, calm animals had a longer carcass, a higher slaughter and fasting weight, and a normal pH24 (<5.7). However, carcass yield was significantly higher in nervous than in restless animals, but did not differ from that of calm steers. It is important to note that these results were obtained under experimental conditions, therefore, effects could have a greater impact on carcass quality under commercial conditions. © 2022 The Author

Comparación de dos Tipos de Recolección en el Cultivo del Algodonero (Gossypium L.) en el Departamento del Atlántico

El presente estudio se realizó en la finca denominada "LOS GUANÁBANOS", la cual se encuentra ubicada en el corregimiento de Cascajal, municipio de Ponedera, Departamento del Atlántico, donde actualmente se explota el cultivo del algodonero. Geográficamente está enmarcada por las siguientes coordenadas 10°32'43" y 10°34´40" de latitud norte; 74°49'20" y 74°51'30" de longitud oeste del meridiano de Greenwich. Los objetivos básicos trazados para llevar a cabo este estudio fueron, determinar qué sistema de recolección de algodón se desarrolló con menores costos para obtener o mejorar la calidad y rendimiento del algodón cosechado. El trabajo se hizo con base en los datos sacados en dicha explotación durante el desarrollo de la recolección manual y la mecánica de algodón, a través de visitas diarias que a este lugar se efectuaron durante el período de la recolección, así como los resultados obtenidos en los centros de desmote y clasificación para obtener los rendimientos Y calidades de los algodones cosechados. La recolección manual de algodón fue más rápida y eficiente que la recolección mecánica. Se obtuvieron mejores rendimientos en algodón de fibra y semilla, entre 1.8% y 1.4% mayor en el algodón cosechado mecánicamente, respecto al recogido manualmente, pero éste último se clasificó de 1/4 a 1/2 grado mayor que el cosechado con la máquina. Recoger un kilogramo de algodón semilla manualmente tiene un costo de $16.84 y mecánicamente$22.42, mientras se percibe un ingreso por la venta de $59.64 por el primero y$61.92 por el segundo. Por otro lado, por cada unidad de costo que se incremente en el sistema manual habrá que disminuir 0.73 de la mecánica, así, si se quiere obtener ingresos por una unidad más de algodón cosechado manualmente, se dejará de obtener 0.96 del algodón recogido mecánicamente

Cardiac troponin and natriuretic peptide analytical interferences from hemolysis and biotin: educational aids from the IFCC Committee on Cardiac Biomarkers (IFCC C-CB).

Two interferences recently brought to the forefront as patient safety issues include hemolysis (hemoglobin) and biotin (vitamin B7). The International Federation for Clinical Chemistry Committee on Cardiac Biomarkers (IFCC-CB) obtained input from a majority of cTn and NP assay manufacturers to collate information related to high-sensitivity (hs)-cTnI, hs-cTnT, contemporary, and POC cTn assays, and NP assays interferences due to hemolysis and biotin. The information contained in these tables was designed as educational tools to aid laboratory professionals and clinicians in troubleshooting cardiac biomarker analytical results that are discordant with the clinical situation