601 research outputs found
Amylase release from streptolysin O-permeabilized pancreatic acinar cells. Effects of Ca2+, guanosine 5'-[gamma-thio]triphosphate, cyclic AMP, tetanus toxin and botulinum A toxin
The molecular requirements for amylase release and the intracellular effects of botulinum A toxin and tetanus toxin on amylase release were investigated using rat pancreatic acinar cells permeabilized with streptolysin O. Micromolar concentrations of free Ca2+ evoked amylase release from these cells. Maximal release was observed in the presence of 30 microM free Ca2+. Ca(2+)-stimulated, but not basal, amylase release was enhanced by guanosine 5'-[gamma-thio]triphosphate (GTP[S]) (3-4 fold) or cyclic AMP (1.5-2 fold). Neither the two-chain forms of botulinum A toxin and tetanus toxin, under reducing conditions, nor the light chains of tetanus toxin, inhibited amylase release triggered by Ca2+, or combinations of Ca2+ + GTP[S] or Ca2+ + cAMP. The lack of inhibition was not due to inactivation of botulinum A toxin or tetanus toxin by pancreatic acinar cell proteolytic enzymes, as toxins previously incubated with permeabilized pancreatic acinar cells inhibited Ca(2+)-stimulated [3H]noradrenaline release from streptolysin O-permeabilized adrenal chromaffin cells. These data imply that clostridial neurotoxins inhibit a Ca(2+)-dependent mechanism which promotes exocytosis in neural and endocrine cells, but not in exocrine cells
Kinetic and Spectroscopic Characterisation of Highly Reactive Methanesulfonates. Leaving Group Effects for Solvolyses and Comments on Geminal Electronic Effects Influencing S1 Reactivity
Highly reactive methanesulfonates (mesylates, ROMs) have been prepared from 1-phenylethanol. cyclohex-2-en-1-ol, diphenylmethanol and p-methoxybenzyl alcohol by treatment with methanesulfonyl chloride and triethylamine in dichloro- or trichloro-methane at - 20 to 0 °C. The mesylates. characterised in solution by H and C NMR at -20 °C, were obtained in satisfactory purity (ca. 95%) in cold solutions but they decomposed by reaction with chloride, triethylamine or the parent alcohol. Rate constants for solvolyses in aqueous acetone and aqueous ethanol have been determined by a fast response conductimetric method. Product selectivities for solvolyses of pmethoxybenzyl mesylate in aqueous ethanol and methanol at 0 °C have been determined by HPLC. From additional new or Iiterature kinetic data for solvolyses of corresponding bromides. chlorides and p-nitrobenzoates (OPNB). Br/CI. OMs/Br and OMs/OPNB rate ratios were calculated; the results are consistent with electronic effects stabilising the carbocationic transition states and increasing OMs/Br rate ratios for these SN 1 solvolyses; none of the evidence supports a geminal electronic effect on Br/CI rate ratios (e.g. caused by stabilisation of the initial state in pmethoxybenzyl chloride). Steric effects on ester /halide rate ratios for solvolyses of tertiary substrates are confirmed. Relative rates over a 10 range for ester and halide leaving groups are evaluated for solvolyses of 1-phenylethyl substrates in 80% ethanol-water. updating previous work by Noyce et al. (1972)
Exact Solutions of the Duffin Kemmer Petiau Equation for the Deformed Hulthen Potential
Using the Nikiforov Uvarov method, an application of the relativistic Duffin
Kemmer Petiau equation in the presence of a deformed Hulthen potential is
presented for spin zero particles. We derived the first order coupled
differential radial equations which enable the energy eigenvalues as well as
the full wavefunctions to be evaluated by using of the Nikiforov Uvarov method
that can be written in terms of the hypergeometric polynomials.Comment: 8 pages. submitted to Physica Script
Alternative evolutionary paths to bacterial antibiotic resistance cause distinct collateral effects.
Published onlineJournal ArticleThis is the author accepted manuscript. The final version is available from Oxford University Press via the DOI in this record.When bacteria evolve resistance against a particular antibiotic, they may simultaneously gain increased sensitivity against a second one. Such collateral sensitivity may be exploited to develop novel, sustainable antibiotic treatment strategies aimed at containing the current, dramatic spread of drug resistance. To date, the presence and molecular basis of collateral sensitivity has only been studied in few bacterial species and is unknown for opportunistic human pathogens such as Pseudomonas aeruginosa. In the present study, we assessed patterns of collateral effects by experimentally evolving 160 independent populations of P. aeruginosa to high levels of resistance against eight commonly used antibiotics. The bacteria evolved resistance rapidly and expressed both collateral sensitivity and cross-resistance. The pattern of such collateral effects differed to those previously reported for other bacterial species, suggesting inter-specific differences in the underlying evolutionary trade-offs. Intriguingly, we also identified contrasting patterns of collateral sensitivity and cross-resistance among the replicate populations adapted to the same drug. Whole-genome sequencing of 81 independently evolved populations revealed distinct evolutionary paths of resistance to the selective drug, which determined whether bacteria became cross-resistant or collaterally sensitive towards others. Based on genomic and functional genetic analysis, we demonstrate that collateral sensitivity can result from resistance mutations in regulatory genes such as nalC or mexZ, which mediate aminoglycoside sensitivity in β-lactam-adapted populations, or the two-component regulatory system gene pmrB, which enhances penicillin sensitivity in gentamicin-resistant populations. Our findings highlight substantial variation in the evolved collateral effects among replicates, which in turn determine their potential in antibiotic therapy.We thank Anette Friedrichs, Lutz Becks, and the Schulenburg group for valuable advice and Melanie Vollstedt for technical support during genome sequencing. We are grateful for financial support from the German Science Foundation (DFG grant SCHU 1415/12-1) and the International Max-Planck Research School for Evolutionary Biology at the University of Kiel. We acknowledge infrastructural support by the DFG excellence cluster Inflammation at Interfaces
Relativistic Aharonov-Casher Phase in Spin One
The Aharonov-Casher (AC) phase is calculated in relativistic wave equations
of spin one. The AC phase has previously been calculated from the Dirac-Pauli
equation using a gauge-like technique \cite{MK1,MK2}. In the spin-one case, we
use Kemmer theory (a Dirac-like particle theory) to calculate the phase in a
similar manner. However the vector formalism, the Proca theory, is more widely
known and used. In the presence of an electromagnetic field, the two theories
are `equivalent' and may be transformed into one another. We adapt these
transformations to show that the Kemmer theory results apply to the Proca
theory. Then we calculate the Aharonov-Casher phase for spin-one particles
directly in the Proca formalism.Comment: 12 page
Status of a DEPFET pixel system for the ILC vertex detector
We have developed a prototype system for the ILC vertex detector based on
DEPFET pixels. The system operates a 128x64 matrix (with ~35x25 square micron
large pixels) and uses two dedicated microchips, the SWITCHER II chip for
matrix steering and the CURO II chip for readout. The system development has
been driven by the final ILC requirements which above all demand a detector
thinned to 50 micron and a row wise read out with line rates of 20MHz and more.
The targeted noise performance for the DEPFET technology is in the range of
ENC=100 e-. The functionality of the system has been demonstrated using
different radioactive sources in an energy range from 6 to 40keV. In recent
test beam experiments using 6GeV electrons, a signal-to-noise ratio of S/N~120
has been achieved with present sensors being 450 micron thick. For improved
DEPFET systems using 50 micron thin sensors in future, a signal-to-noise of 40
is expected.Comment: Invited poster at the International Symposium on the Development of
Detectors for Particle, AstroParticle and Synchrotron Radiation Experiments,
Stanford CA (SNIC06) 6 pages, 12 eps figure
Exact Solution of Photon Equation in Stationary G\"{o}del-type and G\"{o}del Space-Times
In this work the photon equation (massless Duffin-Kemmer-Petiau equation) is
written expilicitly for general type of stationary G\"{o}del space-times and is
solved exactly for G\"{o}del-type and G\"{o}del space-times. Harmonic
oscillator behaviour of the solutions is discussed and energy spectrum of
photon is obtained.Comment: 9 pages,RevTeX, no figure, revised for publicatio
Anomalously interacting new extra vector bosons and their first LHC constraints
In this review phenomenological consequences of the Standard Model extension
by means of new spin-1 chiral fields with the internal quantum numbers of the
electroweak Higgs doublets are summarized. The prospects for resonance
production and detection of the chiral vector and bosons at
the LHC energies are considered. The boson can be observed as a
Breit-Wigner resonance peak in the invariant dilepton mass distributions in the
same way as the well-known extra gauge bosons. However, the bosons
have unique signatures in transverse momentum, angular and pseudorapidity
distributions of the final leptons, which allow one to distinguish them from
other heavy neutral resonances. In 2010, with 40 pb of the LHC
proton-proton data at the energy 7 TeV, the ATLAS detector was used to search
for narrow resonances in the invariant mass spectrum of and
final states and high-mass charged states decaying to a charged
lepton and a neutrino. No statistically significant excess above the Standard
Model expectation was observed. The exclusion mass limits of 1.15 TeV and
1.35 TeV were obtained for the chiral neutral and charged
bosons, respectively. These are the first direct limits on the and
boson production. For almost all currently considered exotic models the
relevant signal is expected in the central dijet rapidity region. On the
contrary, the chiral bosons do not contribute to this region but produce an
excess of dijet events far away from it. For these bosons the appropriate
kinematic restrictions lead to a dip in the centrality ratio distribution over
the dijet invariant mass instead of a bump expected in the most exotic models.Comment: 24 pages, 34 figure, based on talk given by V.A.Bednyakov at 15th
Lomonosov conference, 22.08.201
On Equivalence of Duffin-Kemmer-Petiau and Klein-Gordon Equations
A strict proof of equivalence between Duffin-Kemmer-Petiau (DKP) and
Klein-Gordon (KG) theories is presented for physical S-matrix elements in the
case of charged scalar particles interacting in minimal way with an external or
quantized electromagnetic field. First, Hamiltonian canonical approach to DKP
theory is developed in both component and matrix form. The theory is then
quantized through the construction of the generating functional for Green
functions (GF) and the physical matrix elements of S-matrix are proved to be
relativistic invariants. The equivalence between both theories is then proved
using the connection between GF and the elements of S-matrix, including the
case of only many photons states, and for more general conditions - so called
reduction formulas of Lehmann, Symanzik, Zimmermann.Comment: 23 pages, no figures, requires macro tcilate
On the quantum analogue of Galileo's leaning tower experiment
The quantum analogue of Galileo's leaning tower experiment is revisited using
wave packets evolving under the gravitational potential. We first calculate the
position detection probabilities for particles projected upwards against
gravity around the classical turning point and also around the point of initial
projection, which exhibit mass dependence at both these points. We then compute
the mean arrival time of freely falling particles using the quantum probability
current, which also turns out to be mass dependent. The mass dependence of both
the position detection probabilities and the mean arrival time vanish in the
limit of large mass. Thus, compatibility between the weak equivalence principle
and quantum mechanics is recovered in the macroscopic limit of the latter.Comment: Latex, 12 pages, 1 figure, uses IOP style, clarifications and
references adde
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