MUC1 positive cutaneous metastasis with transepidermal elimination from a breast carcinoma

Breast cancer is the most common cause of cutaneous metastases from internal malignancies. Generally, the neoplastic cells are located in the dermis or hypodermis, while a finding of transepidermal elimination on cutaneous metastases is exceptional. In this report we present a patient with perforating cutaneous metastases from breast cancer with mucin 1 expression. Cutaneous, bone, lung, and hepatic lesions were detected two years after the diagnosis of the primary tumor.Fil: Luna, Amalia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Merino, María Emilia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Alberdi, Cecilio G.. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Abba, Martín Carlos. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segal Eiras, Amada. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; ArgentinaFil: Croce, María Virginia. Universidad Nacional de La Plata. Facultad de Ciencias Médicas. Centro de Investigaciones Inmunológicas Básicas y Aplicadas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentin

An Evidence-Based Survey on Full-Scale Membrane Biological Reactors: Main Technical Features and Operational Aspects

This paper presents the results of a survey on full-scale membrane biological reactors (MBRs) wastewater treatment plants (WWTPs) in Italy. Alongside the main technical characteristics of the Italian MBR plants, the opinions of the plant managers on the operational advantages and disadvantages are described. As reported by the MBR technology suppliers, approximately 290 MBR municipal or industrial WWTPs are in operation in Italy, out of which 242 were studied in this survey. Data from more than one hundred municipal WWTPs were collected; these account for a total capacity of about 2,000,000 population equivalent (PE), which corresponds to 3% of the total organic load treated by the Italian WWTPs with secondary and advanced treatment. Usually, small installations adopt the flat-sheet rather than hollow-fiber membrane configuration. The main reasons why the MBR technology has been preferred to other options are its potential to be used for increasing the treatment capacity of existing plants and its compactness. Moreover, the followed operational advantages have been highlighted: easiness to comply with the discharge limits, removal of pathogens without specific disinfection units, possibility of internal reuse of the effluent, and process automation. Membrane fouling and plant shutdown have been recorded as the most relevant troubles, the last one indeed occurring only occasionally or rarely

Comparative yield and characterization of flavonoids from the stem back and root of Blighia sapida

Flavonoids represent a wide spread group of water soluble phenolic derivatives, known for their medicinal effect. This paper is focused on identifying the functional groups present in flavonoids of Blighia sapida stem bark and root sample. The percentage yield was higher with methanol extract of the stem bark compared to the root. The GC-MS of methanol extract shows carboxylic acid which might be attributed to presence of lipids. The isolation of flavonoids by TLC and the FITR revealed the functional group –OH, C=O,CH which are majorly responsible for the medicinal effect.Keywords: Blighia sapida, characterization, comparative yield, Flavonoids, methanol extrac

Secure and Differentially Private Detection of Net Neutrality Violations by Means of Crowdsourced Measurements

Evaluating Network Neutrality requires comparing the quality of service experienced by multiple users served by different Internet Service Providers. Consequently, the issue of guaranteeing privacy-friendly network measurements has recently gained increasing interest. In this paper we propose a system which gathers throughput measurements from users of various applications and Internet services and stores it in a crowdsourced database, which can be queried by the users themselves to verify if their submitted measurements are compliant with the hypothesis of a neutral network. Since the crowdsourced data may disclose sensitive information about users and their habits, thus leading to potential privacy leakages, we adopt a privacy-preserving method based on randomized sampling and suppression of small clusters. Numerical results show that the proposed solution ensures a good trade-off between usefulness of the system, in terms of precision and recall of discriminated users, and privacy, in terms of differential privacy

Characterization of a P-Rex1 gene signature in breast cancer cells

The Rac nucleotide Exchange Factor (Rac-GEF) P-Rex1 is highly expressed in breast cancer, specifically in the luminal subtype, and is an essential mediator of actin cytoskeleton reorganization and cell migratory responses induced by stimulation of ErbB and other tyrosine-kinase receptors. Heregulin (HRG), a growth factor highly expressed in mammary tumors, causes the activation of P-Rex1 and Rac1 in breast cancer cells via ErbB3, leading to a motile response. Since there is limited information about P-Rex1 downstream effectors, we carried out a microarray analysis to identify genes regulated by this Rac-GEF after stimulation of ErbB3 with HRG. In T-47D breast cancer cells, HRG treatment caused major changes in gene expression, including genes associated with motility, adhesion, invasiveness and metastasis. Silencing P-Rex1 expression from T-47D cells using RNAi altered the induction and repression of a subset of HRG-regulated genes, among them genes associated with extracellular matrix organization, migration, and chemotaxis. HRG induction of MMP10 (matrix metalloproteinase 10) was found to be highly sensitive both to P-Rex1 depletion and inhibition of Rac1 function by the GTPase Activating Protein (GAP) β2-chimaerin, suggesting the dependence of the P-Rex1/Rac1 pathway for the induction of genes critical for breast cancer invasiveness. Notably, there is a significant association in the expression of P-Rex1 and MMP10 in human luminal breast cancer, and their coexpression is indicative of poor prognosis.Facultad de Ciencias MédicasCentro de Investigaciones Inmunológicas Básicas y Aplicada

DFT and PM3 Computational Studies of the Reaction Mechanism of the Oxidation of L-Tyrosine by Iodine in the Gas Phase

- The oxidation of L-Tyrosine by molecular iodine was studied using semi-empirical and density functional theory methods. Molecular information such as net charges, values of frontier orbital energies, composition, proportions and bonding contribution were obtained and analyzed. Thus, possible reactive sites were proposed and the reaction mechanism was postulated. The postulated transition states, intermediates and products were also computed using the PM3 and DFT methods. Computed enthalpies of the oxidation reaction at standard conditions for the PM3 and DFT calculation were 216.97 kJ/mol and -36327404.72 kJ/mol respectively. The calculated ΔGo andΔSo, for the transition states according to the DFT model were both large and negative indicating that the processes were exergonic associative substitution reactions

New occurrence records of <i>Chaetophractus villosus</i> in the Carcarañá River basin, southern Santa Fe province, Argentina

En el presente trabajo se reportan 50 nuevos registros de Chaetophractus villosus para el sur de la provincia de Santa Fe (Argentina) y se los relaciona con algunas variables ambientales. La zona de estudio está ubicada en la cuenca del río Carcarañá, dentro de la región pampeana, uno de los ecosistemas naturales más modificados del mundo. Chaetophractus villosus, vulgarmente conocido como peludo, fue registrado en dos de las cuatro unidades ambientales muestreadas; 42 evidencias se encontraron en las tierras de cultivo y ocho en el bosque xerófilo. Existieron diferencias significativas en la cantidad de evidencias encontradas en la comparación entre estaciones del año y ambiente. La abundancia relativa entre los ambientes fue casi idéntica (tierras de cultivo: 0,17 rastros/km; bosque xerófilo: 0,16 rastros/km). Sin embargo, se pudo establecer un uso diferencial de los ambientes, encontrando registros del peludo en los bosques xerófilos sólo durante las estaciones otoño e invierno, lo que denota una importancia de las áreas forestadas como refugio cuando los campos agrícolas no tienen alta cobertura de vegetaciónWe report 50 new records of Chaetophractus villosus for southern Santa Fe Province (Argentina) and discuss their relationship with environmental variables. The study area is located in the Carcarañá River basin within the Pampean ecoregion, one of the most modified natural ecosystems in the world. The large hairy armadillo was recorded in two of the four environmental units sampled, with 42 records found in cropland and eight in dry woodland. The number of evidences varied between environments and seasons. The relative abundance was nearly identical in different environments (farmland: 0.17 tracks/km; dry woodland: 0.16 tracks/km). There was, however, a differential use of the environments. Records in the dry forests were limited to autumn and winter, which suggests that forested areas are important refuges for large hairy armadillos when farmlands lack high vegetation cover.Facultad de Ciencias Naturales y Muse