Improving top-down proteomics: first steps

Comunicaciones a congreso

Un ejército de mujeres. Un ejército por el agua. Las mujeres indígenas mazahuas en México.

On September, 2003, Mazahua peasants from Villa de Allende,State of México, suffered flooding on 300 hectares of cropsbecause of overflowing of the Malacatepec River, originatingfrom the Villa Victoria dam of the Cutzamala system. Thiswas the reason why the peasants organized themselves todemand payment of their crops. After three months of waiting,in February 2004, the Mazahua began a series of collectiveactions and came together as the Frente para la Defensa de losDerechos Humanos y Recursos Naturales del Pueblo Mazahua.After one year of mobilization, the Ejército Zapatista deMujeres en Defensa del Agua arose, which turned out to havean important media impact that was able to exercise politicalpressure on the instances involved, in benefit of the movement.The objective of this document is to present the most significantfindings regarding the participation of Mazahua people inthe movement. Also, to expose the causes that generated thisimportant political movement, which transcended beyondpayment of crop damages, consolidating other demands such as:provision of drinking water and basic services, as well as a planfor sustainable development for the communities.En septiembre de 2003, campesinos mazahuas de Villa de Allende,Estado de México sufrieron inundaciones en 300 hectáreas decultivo por el desbordamiento del río Malacatepec provenientede la presa de Villa Victoria, del sistema Cutzamala. Esta fue larazón para que los campesinos se organizaran para exigir el pagode sus cultivos. Al cabo de tres meses de espera, en febrero de2004, los mazahuas iniciaron una serie de acciones colectivas y seconstituyeron como el Frente para la Defensa de los Derechos Humanosy Recursos Naturales del Pueblo Mazahua. Después de unaño de movilizaciones surgió el Ejército Zapatista de Mujeres enDefensa del Agua, el cual tuvo un importante impacto mediáticoque logró ejercer presión política ante las instancias involucradas,para beneficio del movimiento. El objetivo de este documentoes presentar los hallazgos más significativos de la participaciónde los mazahuas en la movilización; así como dar a conocer lascausas que generan este importante movimiento político, el cualtrascendió más allá del pago de daños a los cultivos, logrando consolidarotras demandas tales como: la dotación de agua potable yde servicios básicos, así como de un plan de desarrollo sustentablepara las comunidades

Potenziale del metodo Service-Learning per la promozione della cittadinanza e l’educazione del carattere

Promuovere la cittadinanza nelle società odierne significa rafforzare quegli aspetti del carattere delle persone che consentiranno loro di comportarsi in modo responsabile e attivo e di essere coinvolti nel raggiungimento del bene comune. Riteniamo che la principale difficoltà che si trova oggi nell’educazione civica sia la mancanza di etica. Imparare ad essere un buon cittadino è un compito che dura tutta la vita e una saggezza pratica che non richiede solo intelligenza per riconoscere quali tratti migliorano la nostra vita nella società, ma anche la volontà di praticarli. L’educazione del carattere è un modo di comprendere e identificare l’educazione morale. Mira a riaffermare la dimensione etica inevitabile dell’educazione. L’idea di poter educare il carattere delle persone è antica e risale ai tempi della Grecia classica. Ma anche nella nostra società più attuale abbiamo dei punti di riferimento validi. Negli anni Ottanta e Novanta, ad esempio, il concetto di etica della virtù ha ricevuto un grande impulso ed è diventato la base per una buona educazione del carattere contemporaneo. Non è un caso che l’educazione del carattere sia diventata così importante negli ultimi anni, specialmente nel mondo di lingua inglese, parallelamente a una diffusione del metodo Service-Learning.Promoting citizenship in present-day societies means reinforcing those aspects of people’s characters which will allow them to behave responsibly and actively and be involved in achieving the common good. We believe that the main difficulty to be found in civic education nowadays is a lack of ethics. Learning to be a good citizen is a lifelong task and practical wisdom which not only demands intelligence to recognize which traits improve our life in society but also the will to practice them. Character education is a way of understanding and identifying moral education. It aims to reaffirm the inescapable ethical dimension of education. This proposal has its origins in classical Greece, but it was not until the 1980s and 90s that the ethics of virtue received a great boost and became the basis for a good deal of contemporary character education. It is not by chance that character education has grown so much in importance over the last few years, especially in the English-speaking world, in parallel with an increase in Service-Learning methodology

36th Cordoba Guitar Festival: Spectator Analysis Using Structural Equation Modelling (SEM)

The Cordoba Guitar Festival is one of the most important cultural events in Spain. This article analyses the musical preferences, satisfaction, attitudinal loyalty, and behavioural loyalty of spectators who attended the 36th festival held in July 2016, as well as the festival’s economic impact on the city. These characteristics of the public give rise to the four hypotheses of this study. To achieve this aim, a structural equation model (SEM) was used. The results −χ2 = 27.79, with the p-value found to be p = P (χ2 ≥ 27.79) = 0.224—show the goodness-of-fit of the model and indicate that the observed data do fit the expected dataset. The results therefore allow us to accept the proposed hypotheses concerning the influence of different latent factors, such as musical preferences, loyalty, and satisfaction with the festival. This is the main focus with which the present study has been written. Regarding the economic impact, the Festival raised 1.2 million euros, that is, slightly more than 73,000 euros per day. The amount, although not very high, is important as additional income for the city. The conclusions show the need to consider the preferences of the spectator as an essential tool to enable the continuation and longevity of the Cordoba Guitar Festival—an important cultural destination

Laboratory Tests to Assess Optimal Agricultural Residue Traits for an Abrasive Weed Control System

International Conference of Agricultural Engineering Aarhus, Denmark 26 - 29 June (2016)One of the biggest challenges to organic agricultural production and herbicide resistant crops in industrialized countries today is the non-chemical control of weed plants. Studies of new tools and methods for weed control have been motivated by an increased consumer demand for organic produce and consumer and regulatory demands for a reduction in environmentally harmful herbicide use. The objective of this study is to assess different agricultural residues as gritty weed-abrading materials that are delivered through condensed-air machinery. This is a new weed control technology based on highly-directed air-propelled, innocuous, abrasive grit. Laboratory equipment was designed to calculate the angle of repose of seven different agricultural residues (crushed olive seed, walnut shell, maize cob, poultry manure, soybean seed, almond shell and grape seed). Color, digital, high-speed computer vision analysis of the motion and energy of the air-propelled particles was conducted. The high-speed video analysis determined the capability of each grit to damage a reference surface and kill weeds (species of Amaranthus, Centaurea, and Chenopodium) at different growth stages. A preliminary laboratory trial showed that walnut shell grit has great potential to damage/break the reference surface when it was propelled at 600, 700 and 800 kPa air pressure. Abrasive-weeding reduced final weed biomass by 80% compared with the weedy control at early growth stages. Field research tests are needed in different cropping systems to improve the technical and economic efficiency of this novel system before on-farm adoption

Programa de rehabilitación cardíaca mediante un entrenamiento de tenis adaptado

The aims of this study were to determine the effect of a cardiac rehabilitation program, based on the adjustment of a sports modality (tennis), on differentes laboratory analysis variables (triglycerides, cholesterol,cholesterol LDL, cholesterol HDL and glucose ) And and on an exercise stress test (metabolics equivalent-METs-, time of effort, systolic pressure, dyastolic pressure, maximum cardiac frecuencia and double product). The study involved 7 patients with low risk myocardial heart attack. The ages was include between 48 and 63 years old. By the end of the program, which has lasted 3 months, triglycerides, cholesterol, exercise capacity and double product had improved significantly (p<.005 for all).Los objetivos de este estudio son evaluar los efectos de nuestro programa de rehabilitación cardiaca, basado en la adaptación de una modalidad deportiva (tenis), sobre diferentes variables del perfil lipídico (triglicéridos, colesterol, cLDL, cHDL, y glucosa) así como en la prueba de esfuerzo (MET, tiempo de esfuerzo, presión sistólica, presión diastólica, frecuencia cardíaca máxima y doble producto). Analizamos a 7 pacientes con infarto agudo de miocardio de bajo riesgo. Las edades estaban comprendidas entre los 48 y 63 años. Tras la realización del programa, que ha tenido 3 meses de duración, se han producido mejoras significativas a nivel de triglicéridos, colesterol, capacidad funcional valorada en equivalentes metabólicos y doble producto (p<.005 para todos)

Occurrence of antibiotics and bacterial resistance in wastewater and sea water from the Antarctic

The potential presence of introduced antibiotics in the aquatic environment is a hot topic of concern, particularly in the Antarctic, a highly vulnerable area protected under the Madrid protocol. The increasing presence of human population, especially during summer, might led to the appearance of pharmaceuticals in wastewater. The previous discovery of Escherichia coli strains resistant to antibiotics in sea water and wastewater collected in King George Island motivated our investigation on antibiotics occurrence in these samples. The application of a multi-residue LCMS/MS method for 20 antibiotics, revealed the presence of 8 compounds in treated wastewater, mainly the quinolones ciprofloxacin and norfloxacin (92% and 54% of the samples analyzed, average concentrations 0.89 μg/L and 0.75 μg/L, respectively) and the macrolides azithromycin and clarithromycin (15% positive samples, and average concentrations near 0.4 μg/L), and erythromycin (38% positive samples, average concentration 0.003 μg/L). Metronidazole and clindamycin were found in one sample, at 0.17 and 0.1 μg/L, respectively; and trimethoprim in two samples, at 0.001 μg/L. Analysis of sea water collected near the outfall of the wastewater discharges also showed the sporadic presence of 3 antibiotics (ciprofloxacin, clindamycin, trimethoprim) at low ng/L level, illustrating the impact of pharmaceuticals consumption and the poor removal of these compounds in conventional WWTPs. The most widespread antibiotic in sea water was ciprofloxacin, which was found in 15 out of 34 sea water samples analyzed, at concentrations ranging from 4 to 218 ng/L. Bacteria resistance was observed for some antibiotics identified in the samples (e.g. trimetropim and nalidixic acid –a first generation quinolone). However, resistance to some groups of antibiotics could not be correlated to their presence in the water samples due to analytical limitations (penicillins, tetraciclines). On the contrary, for some groups of antibiotics detected in samples (macrolides), the antibacterial activity against E. Coli was not investigated because these antibiotics do not include this bacterial species in their spectrum of activity. Our preliminary data demonstrate that antibiotics occurrence in the Antarctic aquatic environment is an issue that needs to be properly addressed. Periodical monitoring of water samples and the implementation of additional treatments in the WWTPs are recommended as a first step to prevent potential problems related to the presence of antibiotics and other emerging contaminants in the near future in Antarctica

Carcinoma adrenocortical con trombosis extensa: reporte de un caso

El carcinoma adrenocortical (CAC) es un tumor maligno poco frecuente. La presentación clínica, por lo general, es por exceso de producción hormonal. La afectación del sistema venoso con trombos en la vena cava inferior (VCI) solo se reporta en un tercio de los casos. El presente estudio informa un caso de CAC con producción hormonal con trombo tumoral con extensión a la VCI y aurícula derecha asociado a embolia pulmonar. Se trata de una paciente de género femenino de 63 años con diagnóstico reciente de hipertensión arterial en manejo, quien consulta a urgencias por dolor abdominal asociado a náuseas, vómitos y pérdida de peso. Imágenes convencionales con hallazgos de masa en fosa suprarrenal derecha irresecable sin plano de clivaje para realizar algún tipo de procedimiento. Patología con diagnóstico de neoplasia de la corteza suprarrenal con extensa necrosis. Se inicia mitotano y esquema ambulatorio de quimioterapia

Ocular Rosai-Dorfman disease and juvenile xanthogranuloma present at the same time in a young girl. A clinicopathologic report

ResumenProp&oacute;sito: Informar el caso de una paciente joven con dos trastornos histioc&iacute;ticos simult&aacute;neos: enfermedad de RosaiDorfman y xantogranuloma juvenil. M&eacute;todos: Examinamos una adolescente de 15 a&ntilde;os con m&uacute;ltiples masas de la conjuntiva epibulbar de ambos ojos sin aparente compromiso nodal y en qui&eacute;n se realiz&oacute; resecci&oacute;n parcial de las lesiones. Resultados: El examen f&iacute;sico fue normal, sinevidencia de linfadenopat&iacute;as. El diagn&oacute;stico fue confirmado con histopatolog&iacute;a y an&aacute;lisis histoqu&iacute;mico de las lesiones removidas. Las dem&aacute;s masas respondieron satisfactoriamente a la terapia con esteroides. No se ha encontrado evidencia posterior de ninguna de las dos entidades. Conclusi&oacute;n: Tanto la enfermedad de Rosai-Dorfman como el xantogranuloma juvenil pueden presentarse juntos comotumoraciones epibulbares sin cualesquiera otro compromiso sist&eacute;mico.[D&iacute;az AL, G&oacute;mez AJ, Serrano-Calder&oacute;n C, Fuentes VC. Enfermedad de Rosai-Dorfman y xantogranuloma juvenil ocular simult&aacute;neas en una adolescente. Un informe clinicopatol&oacute;gico. MedUNAB 2007; 10:130-132].Key words: Enfermedad de Rosai-Dorfman, Xantogra nuloma juvenil, Histiocitosis, neoplasias conjuntivales, Ojo

Zebrafish modeling reveals that SPINT1 regulates the aggressiveness of skin cutaneous melanoma and its crosstalk with tumor immune microenvironment

Background Skin cutaneous melanoma (SKCM) is the most lethal form of skin cancer and while incidence rates are declining for most cancers, they have been steadily rising for SKCM. Serine protease inhibitor, kunitz-type, 1 (SPINT1) is a type II transmembrane serine protease inhibitor that has been shown to be involved in the development of several types of cancer, such as squamous cell carcinoma and colorectal cancer. Methods We used the unique advantages of the zebrafish to model the impact of Spint1a deficiency in early transformation, progression and metastatic invasion of SKCM together with in silico analysis of the occurrence and relevance of SPINT1 genetic alterations of the SKCM TCGA cohort. Results We report here a high prevalence of SPINT1 genetic alterations in SKCM patients and their association with altered tumor immune microenvironment and poor patient survival. The zebrafish model reveals that Spint1a deficiency facilitates oncogenic transformation, regulates the tumor immune microenvironment crosstalk, accelerates the onset of SKCM and promotes metastatic invasion. Notably, Spint1a deficiency is required at both cell autonomous and non-autonomous levels to enhance invasiveness of SKCM. Conclusions These results reveal a novel therapeutic target for SKCM