1,536 research outputs found
Economic sanctions against Russia: italian point of view
This article is devoted to the impact of economic sanctions against Russia imposed by the European Union on the economy of Italy and reflects the views of the Italians. Special attention is paid to the losses incurred by the economy of Italy
Kinetic Solvers with Adaptive Mesh in Phase Space
An Adaptive Mesh in Phase Space (AMPS) methodology has been developed for
solving multi-dimensional kinetic equations by the discrete velocity method. A
Cartesian mesh for both configuration (r) and velocity (v) spaces is produced
using a tree of trees data structure. The mesh in r-space is automatically
generated around embedded boundaries and dynamically adapted to local solution
properties. The mesh in v-space is created on-the-fly for each cell in r-space.
Mappings between neighboring v-space trees implemented for the advection
operator in configuration space. We have developed new algorithms for solving
the full Boltzmann and linear Boltzmann equations with AMPS. Several recent
innovations were used to calculate the discrete Boltzmann collision integral
with dynamically adaptive mesh in velocity space: importance sampling,
multi-point projection method, and the variance reduction method. We have
developed an efficient algorithm for calculating the linear Boltzmann collision
integral for elastic and inelastic collisions in a Lorentz gas. New AMPS
technique has been demonstrated for simulations of hypersonic rarefied gas
flows, ion and electron kinetics in weakly ionized plasma, radiation and light
particle transport through thin films, and electron streaming in
semiconductors. We have shown that AMPS allows minimizing the number of cells
in phase space to reduce computational cost and memory usage for solving
challenging kinetic problems
Investigation of Using Natural Aromatizers of Lemon Essential Oil in Ayurvedic Culinary
There was considered a possibility of using natural aromatizers in ayurvedic culinary, namely fractions that act as a product of lemon essential oil. There was presented the component ratio and characteristic of a fragrance of a terpene fraction and terpenoid one, obtained by the gas-chromatographic analysis on a nozzle column with a stable dynonyl phthalate fraction by the standard method.A classic salad sauce βVinaigretteβ was chosen as a research subject. The introduction of natural aromatizers was conducted at the final stage of mixing olive oil and other ingredients of the dish. The optimal amount of aromatizers was determined empirically by sensor analysis results. During the studies, it has been established, that a fraction of lemon essential oil No. 1 must be introduced in amount 0,1 g for 1000 g of the ready product, a fraction of lemon essential oil No. 2 β 0,125 g for 1000 g of the ready product.As far as terpenes and terpenoids of lemon essential oil are natural substances of the initial raw material, stimulating food juices extraction, it allows to state about the activation of so-called βdigestive fireβ at using the aromatized sauce
Implification of ephippium analysis (Cladocera, branchiopoda, crustacea) for reconstruction of past environmental changes in central yakutia, Russia
Β© SGEM2017. All Rights Reserved. The aim of our investigation is to reconstruct the local and regional palaeoenvironmental conditions and to highlight the rapid evolution of the thermokarst lake during the Holocene climate optimum. The investigated lake was located in Central Yakutia, Siberia, Russia. The investigated core was collected in a small pingo within a large Central Yakutian thermokarst Khara Bulgunnyakh basin (alas). According to ephippium analysis the formation of the lake coincided with the Holocene climatic optimum. Using cluster analysis we identified three statistically significant ecological zones that reflected changes in the species composition of sub-fossil cladoceran communities and sharp increase in concentrations of ephippia per sample. The period of optimal conditions for Cladocera that took place between 6500 and 6350 cal. yrs. BP is characterized by complex community structures and numerous resisting eggs of cladoceran remains deposited in sediments. Development of the lake ecosystem was rapid and it disappeared quite quickly
Instructions for laboratory training in Special Microbiology and Virology for students of Faculty of Dentistry
ΠΠΠΠ ΠΠΠΠΠΠΠΠΠ―ΠΠΠ’ΠΠΠΠ§ΠΠ‘ΠΠΠ Π£ΠΠΠΠΠΠΠ―ΠΠΠΠΠ ΠΠ’ΠΠ ΠΠ«Π Π ΠΠΠΠ’Π«ΠΠΠ Π£Π‘ΠΠΠΠΠΠ―ΠΠΠΠ‘Π’Π ΠΠΠΠ«Π Π‘Π’Π£ΠΠΠΠ’Π«ΠΠ ΠΠΠ’ΠΠ§ΠΠ‘ΠΠΠ ΠΠΠΠ«ΠΠΠ‘ΠΎΠ΄Π΅ΡΠΆΠ°ΡΡΡ ΠΏΠ»Π°Π½, ΠΏΡΠΎΠ³ΡΠ°ΠΌΠΌΠ° ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΡ
Π·Π°Π½ΡΡΠΈΠΉ ΠΈ ΠΎΡΠ½ΠΎΠ²Π½ΡΠ΅ ΠΏΡΠ°ΠΊΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ Π½Π°Π²ΡΠΊΠΈ ΠΏΠΎ ΠΎΠ±ΡΠ΅ΠΉ ΠΌΠΈΠΊΡΠΎΠ±ΠΈΠΎΠ»ΠΎΠ³ΠΈΠΈ ΠΈ Π²ΠΈΡΡΡΠΎΠ»ΠΎΠ³ΠΈΠΈ
ΠΠΎΠΌΠΏΠ΅ΡΠ΅Π½ΡΠΈΡ ΠΎΡΠ³Π°Π½ΠΎΠ² ΠΌΠ΅ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΌΠΎΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ ΠΊΡΡΠΏΠ½Π΅ΠΉΡΠΈΡ Π³ΠΎΡΠΎΠ΄ΠΎΠ² Π² ΡΡΠ΅ΡΠ΅ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½ΠΈΡ ΡΠΎΡΠΈΠ°Π»ΡΠ½ΠΎ-ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΡ
The subject of the article is legal issues of municipal authoritiesβ activities in the field of socioeconomic development.The purpose of the article is to identify trends in the legal regulation of socioeconomic issues of organizing local self-government in the largest cities of the Russian Federation. The methodology includes formal legal interpretation of Russian legislative acts, decisions of Russian Constitutional Court as well as systemic analysis of municipal acts.The main results, scope of application. The article analyzes the division of powers between regional and municipal authorities. At the present stage of development of Russian federalism, a rather complex and unstable model of delimitation of powers between levels of public authority has developed, which does not contribute to the socio-economic development of municipalities. distribution of powers between levels of public authority. The question of the delimitation of powers acquires new content in the formation of urban districts with intracity division and intracity districts in the largest cities. Based on the experience of organization and activities of urban districts with intracity division of Chelyabinsk and Samara, the problems and prospects of this approach to territorial planning and the distribution of powers between levels of public authority are outlined. Special attention is paid to the problem of finding a balance between centralization and decentralization of local self-government. In this aspect, the issue of a more effective delimitation of powers between different levels of public authority comes to the fore in order to maintain a unified urban policy in the field of ensuring the socio-economic development of the largest cities of the Russian Federation.Conclusions. A comprehensive approach is needed to delineate powers between levels of public authority, taking into account the importance of the largest cities in the spatial development of the Russian economy and their role in the formation of urban agglomerations. Only centralized administration makes it possible to develop a unified urban infrastructure. Therefore, improving the interaction of public authorities and local self-government is a necessary condition and the most important area of work in major cities that requires joint efforts. However, at the same time, it is necessary to avoid the degeneration of local self-government into a state one and the loss of the internal content of this institution of public power.ΠΠ° ΠΎΡΠ½ΠΎΠ²Π΅ Π²ΡΡΠ²Π»Π΅Π½ΠΈΡ ΡΠ΅Π½Π΄Π΅Π½ΡΠΈΠΉ ΠΏΡΠ°Π²ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ΅Π³ΡΠ»ΠΈΡΠΎΠ²Π°Π½ΠΈΡ Π²ΠΎΠΏΡΠΎΡΠΎΠ² ΠΎΡΠ³Π°Π½ΠΈΠ·Π°ΡΠΈΠΈ ΠΌΠ΅ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΌΠΎΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ Π² ΠΊΡΡΠΏΠ½Π΅ΠΉΡΠΈΡ
Π³ΠΎΡΠΎΠ΄Π°Ρ
Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ Π€Π΅Π΄Π΅ΡΠ°ΡΠΈΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΡΡΡ Π°Π½Π°Π»ΠΈΠ· ΡΠ°Π·Π³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ»Π½ΠΎΠΌΠΎΡΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠ΅Π³ΠΈΠΎΠ½Π°Π»ΡΠ½ΡΠΌΠΈ ΠΈ ΠΌΡΠ½ΠΈΡΠΈΠΏΠ°Π»ΡΠ½ΡΠΌΠΈ ΠΎΡΠ³Π°Π½Π°ΠΌΠΈ Π²Π»Π°ΡΡΠΈ. ΠΡΠ΄Π΅Π»ΡΠ½ΠΎΠ΅ Π²Π½ΠΈΠΌΠ°Π½ΠΈΠ΅ ΡΠ΄Π΅Π»ΡΠ΅ΡΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΠ΅ ΠΏΠΎΠΈΡΠΊΠ° Π±Π°Π»Π°Π½ΡΠ° ΠΌΠ΅ΠΆΠ΄Ρ ΡΠ΅Π½ΡΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ ΠΈ Π΄Π΅ΡΠ΅Π½ΡΡΠ°Π»ΠΈΠ·Π°ΡΠΈΠ΅ΠΉ ΠΌΠ΅ΡΡΠ½ΠΎΠ³ΠΎ ΡΠ°ΠΌΠΎΡΠΏΡΠ°Π²Π»Π΅Π½ΠΈΡ. Π ΡΡΠΎΠΌ Π°ΡΠΏΠ΅ΠΊΡΠ΅ Π½Π° ΠΏΠ΅ΡΠ²ΡΠΉ ΠΏΠ»Π°Π½ Π²ΡΡ
ΠΎΠ΄ΠΈΡ Π²ΠΎΠΏΡΠΎΡ Π±ΠΎΠ»Π΅Π΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠ³ΠΎ ΡΠ°Π·Π³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ»Π½ΠΎΠΌΠΎΡΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρ ΡΠ°Π·Π»ΠΈΡΠ½ΡΠΌΠΈ ΡΡΠΎΠ²Π½ΡΠΌΠΈ ΠΏΡΠ±Π»ΠΈΡΠ½ΠΎΠΉ Π²Π»Π°ΡΡΠΈ Ρ ΡΠ΅Π»ΡΡ ΡΠΎΡ
ΡΠ°Π½Π΅Π½ΠΈΡ Π΅Π΄ΠΈΠ½ΠΎΠΉ Π³ΠΎΡΠΎΠ΄ΡΠΊΠΎΠΉ ΠΏΠΎΠ»ΠΈΡΠΈΠΊΠΈ Π² ΡΡΠ΅ΡΠ΅ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½ΠΈΡ ΡΠΎΡΠΈΠ°Π»ΡΠ½ΠΎ-ΡΠΊΠΎΠ½ΠΎΠΌΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠ°Π·Π²ΠΈΡΠΈΡ ΠΊΡΡΠΏΠ½Π΅ΠΉΡΠΈΡ
Π³ΠΎΡΠΎΠ΄ΠΎΠ² Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ Π€Π΅Π΄Π΅ΡΠ°ΡΠΈΠΈ. Π‘ΡΠΎΡΠΌΡΠ»ΠΈΡΠΎΠ²Π°Π½ Π²ΡΠ²ΠΎΠ΄ ΠΎ Π½Π΅ΠΎΠ±Ρ
ΠΎΠ΄ΠΈΠΌΠΎΡΡΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠ΄Ρ
ΠΎΠ΄Π° ΠΊ ΡΠ°Π·Π³ΡΠ°Π½ΠΈΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ»Π½ΠΎΠΌΠΎΡΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρ ΡΡΠΎΠ²Π½ΡΠΌΠΈ ΠΏΡΠ±Π»ΠΈΡΠ½ΠΎΠΉ Π²Π»Π°ΡΡΠΈ Ρ ΡΡΠ΅ΡΠΎΠΌ Π·Π½Π°ΡΠΈΠΌΠΎΡΡΠΈ ΠΊΡΡΠΏΠ½Π΅ΠΉΡΠΈΡ
Π³ΠΎΡΠΎΠ΄ΠΎΠ² Π² ΠΏΡΠΎΡΡΡΠ°Π½ΡΡΠ²Π΅Π½Π½ΠΎΠΌ ΡΠ°Π·Π²ΠΈΡΠΈΠΈ ΡΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ ΡΠΊΠΎΠ½ΠΎΠΌΠΈΠΊΠΈ ΠΈ ΠΈΡ
ΡΠΎΠ»ΠΈ Π² ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½ΠΈΠΈ Π³ΠΎΡΠΎΠ΄ΡΠΊΠΈΡ
Π°Π³Π»ΠΎΠΌΠ΅ΡΠ°ΡΠΈΠΉ
The organization of the rating system for assessing the quality of university students training
In spite the universities use the rating system of assessing for a quite long time, this issue is still not fully considered. The article is based on the position of insufficient state of knowledge of the multifunctionality of the students rating. The rating helps to reveal the potential of students. It is mentioned that the rating system of assessment is a tool that will improve the quality of training and provide the required level of motivation.
During the research, the following methods were used: analysis, synthesis, induction, deduction. As a result, the principles of the rating assessment system were revealed, a definition of the term "Rating" was given, its goals were indicated. As an example of the organization of the rating system of assessment, the Nyzhnii Novhorod State Pedagogical University and its "Regulations on the rating system for assessing the quality of student training" were used. It is noted that the rating includes: current check, midterm and final examinations. Further, all possible conditions under which the student receives points were identified and considered.
On the example of the NSPU named after Kozma Minin transferring of the rating points into a five-point grading rating system is presented. Also, the types of violations for which points are discarded, are considered. As the study showed, comprehensive planned system yields productive results for students and the university.peer-reviewe
Π‘ΠΈΠ½ΡΠ΅Π· ΡΠ° ΡΡΠ·ΠΈΠΊΠΎ-Ρ ΡΠΌΡΡΠ½Ρ Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΡ Π°Π»ΠΊΡΠ»-2-((5-(1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-1-ΡΠ»)ΠΌΠ΅ΡΠΈΠ»Π΅Π½-4-R-1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»-3-ΡΠ»)ΡΡΠΎ)Π΅ΡΠ°Π½(ΠΏΡΠΎΠΏΠ°Π½,Π±Π΅Π½Π·)ΡΠΌΡΠ΄Π°ΡΡΠ²
The synthesis of new biologically active compounds that will replace expensive foreign medicines at the pharmaceutical market of Ukraine is one of the most important directions in development of modern pharmacy and medicine. The synthesis, study of physicochemical and biological properties of new compounds containing 1,2,4-triazole and 1H-tetrazole cores are important tasks of modern synthetic chemistry.Aim. To synthesize new highly efficient and low-toxic substances, namely alkil-2-((5-(1Π-tetrazole-1-yl)methyl-4-R-1,2,4-triazole-3-yl)thio)etan(propan,benz)imidates, and study physicochemical properties of all compounds synthesized.Results and discussion. Twelve new compounds have been obtained as a result of synthetic transformations, the structure of compounds synthesized has been confirmed by modern complex of physicochemical methods of analysis (IR-spectrophotometry, 1H NMR-spectroscopy, elemental analysis), and their individuality has been proven on an Agilent 1260 Infinity HPLC high-performance liquid chromatograph equipped with an Agilent 6120 mass spectrometer.Experimental part. As starting materials for the synthesis of alkil-2-((5-(1Π-tetrazole-1-yl)methyl-4-R-1,2,4-triazole-3-yl)thio)etan(propan,benz)imidates the corresponding 2-((5-(1Π-tetrazole-1-yl)methyl-4-R-1,2,4-triazole-3-yl)thio)aceto(propane,benzo)nitriles were used. The synthesis was carried out in the absolute alcohol medium (propanol, butanol, octanol or allyl alcohol) with chloroform. Conclusions. During synthetic and physicochemical studies the preparative methods for the synthesis of alkil-2-((5-(1Π-tetrazole-1-yl)methyl-4-R-1,2,4-triazole-3-yl)thio)etan(propan,benz)imidates have been developed, the structure of the compounds synthesized has been determined and finally confirmed.ΠΠ΄Π½ΠΈΠΌ ΠΈΠ· ΠΏΡΠΈΠΎΡΠΈΡΠ΅ΡΠ½ΡΡ
Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½ΠΈΠΉ ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΠΎΠΉ ΡΠ°ΡΠΌΠ°ΡΠΈΠΈ ΠΈ ΠΌΠ΅Π΄ΠΈΡΠΈΠ½Ρ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΠ½ΡΠ΅Π· Π½ΠΎΠ²ΡΡ
ΠΎΡΠ΅ΡΠ΅ΡΡΠ²Π΅Π½Π½ΡΡ
Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈ Π°ΠΊΡΠΈΠ²Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΠΊΠΎΡΠΎΡΡΠ΅ Π±ΡΠ΄ΡΡ Π·Π°ΠΌΠ΅Π½ΡΡΡ Π΄ΠΎΡΠΎΠ³ΠΈΠ΅ Π·Π°ΡΡΠ±Π΅ΠΆΠ½ΡΠ΅ Π°Π½Π°Π»ΠΎΠ³ΠΈ Π½Π° ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠΌ ΡΡΠ½ΠΊΠ΅. Π‘Π΅Π³ΠΎΠ΄Π½Ρ Π°ΠΊΡΡΠ°Π»ΡΠ½ΡΠΌ ΡΠ²Π»ΡΠ΅ΡΡΡ ΡΠΈΠ½ΡΠ΅Π·, ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² Π½ΠΎΠ²ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΠΊΠΎΡΠΎΡΡΠ΅ Π²ΠΊΠ»ΡΡΠ°ΡΡ ΡΠ΄ΡΠ° 1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»Π° ΠΈ 1,2,3,4-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»Π°.Π¦Π΅Π»Ρ. Π¦Π΅Π»Π΅Π½Π°ΠΏΡΠ°Π²Π»Π΅Π½Π½ΡΠΉ ΡΠΈΠ½ΡΠ΅Π· ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΡΠΎΠ΄Π΅ΡΠΆΠ°ΡΠΈΡ
ΡΠ΄ΡΠΎ1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»Π° ΠΈ ΡΠ΄ΡΠΎ 1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»Π°, ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΡΠ²ΠΎΠΉΡΡΠ² ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΈΡ
ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠ΅ ΡΠΈΠ½ΡΠ΅Π·Π° ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΎ 12 Π½ΠΎΠ²ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ, ΡΡΡΠΎΠ΅Π½ΠΈΠ΅ ΠΊΠΎΡΠΎΡΡΡ
ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΎ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° ΡΠΎΠ²ΡΠ΅ΠΌΠ΅Π½Π½ΡΡ
ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΠΎΠ² Π°Π½Π°Π»ΠΈΠ·Π° (ΠΠ-ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠΈΠΈ, 1Π Π―ΠΠ -ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΠΈΠΈ, ΡΠ»Π΅ΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΠΈΠ·Π°), Π° ΠΈΡ
ΠΈΠ½Π΄ΠΈΠ²ΠΈΠ΄ΡΠ°Π»ΡΠ½ΠΎΡΡΡ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½Π° Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠ³ΠΎ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠ° Agilent 1260 Infinity HPLC, ΠΎΠ±ΠΎΡΡΠ΄ΠΎΠ²Π°Π½Π½ΠΎΠ³ΠΎ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΎΠΌ Agilent 6120.ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π°Ρ ΡΠ°ΡΡΡ. ΠΠ»Ρ ΡΠΈΠ½ΡΠ΅Π·Π° Π°Π»ΠΊΠΈΠ»-2-((5-(1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-1-ΠΈΠ»)ΠΌΠ΅ΡΠΈΠ»Π΅Π½-4-R-1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»-3-ΠΈΠ»)ΡΠΈΠΎ)ΡΡΠ°Π½(ΠΏΡΠΎΠΏΠ°Π½,Π±Π΅Π½Π·)ΠΈΠΌΠΈΠ΄Π°ΡΠΎΠ² Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ ΠΈΡΡ
ΠΎΠ΄Π½ΡΡ
Π²Π΅ΡΠ΅ΡΡΠ² ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π»ΠΈ 2-((5-(1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-1-ΠΈΠ»)ΠΌΠ΅ΡΠΈΠ»-4-R-1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»-3-ΠΈΠ»)ΡΠΈΠΎ)Π°ΡΠ΅ΡΠΎ(ΠΏΡΠΎΠΏΠ°Π½,Π±Π΅Π½Π·ΠΎ)Π½ΠΈΡΡΠΈΠ»Ρ. Π‘ΠΈΠ½ΡΠ΅Π· ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Π² ΡΡΠ΅Π΄Π΅ ΡΠΎΠΎΡΠ²Π΅ΡΡΡΠ²ΡΡΡΠ΅Π³ΠΎ Π°Π±ΡΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΡΠ° (ΠΏΡΠΎΠΏΠ°Π½ΠΎΠ»Π°, Π±ΡΡΠ°Π½ΠΎΠ»Π°, ΠΎΠΊΡΠ°Π½ΠΎΠ»Π° ΠΈΠ»ΠΈ Π°Π»Π»ΠΈΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΏΠΈΡΡΠ°) Π² ΠΏΡΠΈΡΡΡΡΡΠ²ΠΈΠΈ Ρ
Π»ΠΎΡΠΎΡΠΎΡΠΌΠ°.ΠΡΠ²ΠΎΠ΄Ρ. ΠΠΎ Π²ΡΠ΅ΠΌΡ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΉ ΡΠ°Π·ΡΠ°Π±ΠΎΡΠ°Π½Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½ΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΡΠΈΠ½ΡΠ΅Π·Π° Π°Π»ΠΊΠΈΠ»-2-((5-(1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-1-ΠΈΠ»)ΠΌΠ΅ΡΠΈΠ»Π΅Π½-4-R-1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»-3-ΠΈΠ»)ΡΠΈΠΎ)ΡΡΠ°Π½(ΠΏΡΠΎΠΏΠ°Π½,Π±Π΅Π½Π·)ΠΈΠΌΠΈΠ΄Π°ΡΠΎΠ², ΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΠΈ ΠΎΠΊΠΎΠ½ΡΠ°ΡΠ΅Π»ΡΠ½ΠΎ ΠΏΠΎΠ΄ΡΠ²Π΅ΡΠΆΠ΄Π΅Π½ΠΎ ΡΡΡΠΎΠ΅Π½ΠΈΠ΅ Π²ΡΠ΅Ρ
ΡΠΈΠ½ΡΠ΅Π·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ.ΠΠ΄Π½ΠΈΠΌ ΡΠ· ΠΏΡΡΠΎΡΠΈΡΠ΅ΡΠ½ΠΈΡ
Π½Π°ΠΏΡΡΠΌΠΊΡΠ² ΡΡΡΠ°ΡΠ½ΠΎΡ ΡΠ°ΡΠΌΠ°ΡΡΡ ΡΠ° ΠΌΠ΅Π΄ΠΈΡΠΈΠ½ΠΈ Ρ ΡΠΈΠ½ΡΠ΅Π· Π½ΠΎΠ²ΠΈΡ
Π²ΡΡΡΠΈΠ·Π½ΡΠ½ΠΈΡ
Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΎ Π°ΠΊΡΠΈΠ²Π½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ, ΡΠΊΡ Π±ΡΠ΄ΡΡΡ Π·Π°ΠΌΡΠ½ΡΠ²Π°ΡΠΈ Π΄ΠΎΡΠΎΠ³Ρ Π·Π°ΠΊΠΎΡΠ΄ΠΎΠ½Π½Ρ Π°Π½Π°Π»ΠΎΠ³ΠΈ Π½Π° ΡΠ°ΡΠΌΠ°ΡΠ΅Π²ΡΠΈΡΠ½ΠΎΠΌΡ ΡΠΈΠ½ΠΊΡ. ΠΠ°Β ΡΠ΅ΠΏΠ΅ΡΡΡΠ½ΡΠΉ ΡΠ°Ρ Π°ΠΊΡΡΠ°Π»ΡΠ½ΠΈΠΌ Ρ ΡΠΈΠ½ΡΠ΅Π·, Π²ΠΈΠ²ΡΠ΅Π½Π½Ρ ΡΡΠ·ΠΈΠΊΠΎ-Ρ
ΡΠΌΡΡΠ½ΠΈΡ
ΡΠ° Π±ΡΠΎΠ»ΠΎΠ³ΡΡΠ½ΠΈΡ
Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΠ΅ΠΉ Π½ΠΎΠ²ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ, ΡΠΊΡ ΠΌΡΡΡΡΡΡ ΡΠ΄ΡΠ° 1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»Ρ ΡΠ° 1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»Ρ.ΠΠ΅ΡΠ°. Π¦ΡΠ»Π΅ΡΠΏΡΡΠΌΠΎΠ²Π°Π½ΠΈΠΉ ΡΠΈΠ½ΡΠ΅Π· ΡΠΏΠΎΠ»ΡΠΊ, ΡΠΎ ΠΌΡΡΡΡΡΡ ΡΠ΄ΡΠΎ 1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»Ρ ΡΠ° ΡΠ΄ΡΠΎ 1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»Ρ, Π²ΠΈΠ²ΡΠ΅Π½Π½Ρ ΡΡΠ·ΠΈΠΊΠΎ-Ρ
ΡΠΌΡΡΠ½ΠΈΡ
Π²Π»Π°ΡΡΠΈΠ²ΠΎΡΡΠ΅ΠΉ ΡΠΈΠ½ΡΠ΅Π·ΠΎΠ²Π°Π½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΠΈ ΡΠ° ΡΡ
ΠΎΠ±Π³ΠΎΠ²ΠΎΡΠ΅Π½Π½Ρ. Π ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ½ΠΈΡ
ΠΏΠ΅ΡΠ΅ΡΠ²ΠΎΡΠ΅Π½Ρ ΠΎΡΡΠΈΠΌΠ°Π½ΠΎ 12 Π½ΠΎΠ²ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ, Π±ΡΠ΄ΠΎΠ²Ρ ΡΠΊΠΈΡ
ΠΏΡΠ΄ΡΠ²Π΅ΡΠ΄ΠΆΠ΅Π½ΠΎ Π·Π° Π΄ΠΎΠΏΠΎΠΌΠΎΠ³ΠΎΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΡ ΡΡΡΠ°ΡΠ½ΠΈΡ
ΡΡΠ·ΠΈΠΊΠΎ-Ρ
ΡΠΌΡΡΠ½ΠΈΡ
ΠΌΠ΅ΡΠΎΠ΄ΡΠ² Π°Π½Π°Π»ΡΠ·Ρ (ΠΠ§-ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΡΡ, 1ΠΒ Π―ΠΠ -ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΊΠΎΠΏΡΡ, Π΅Π»Π΅ΠΌΠ΅Π½ΡΠ½ΠΎΠ³ΠΎ Π°Π½Π°Π»ΡΠ·Ρ), Π° ΡΡ
ΡΠ½Π΄ΠΈΠ²ΡΠ΄ΡΠ°Π»ΡΠ½ΡΡΡΡ ΠΏΡΠ΄ΡΠ²Π΅ΡΠ΄ΠΈΠ»ΠΈ Π½Π° ΡΡΠ΄ΠΈΠ½Π½ΠΎΠΌΡ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΡ Agilent 1260 Infinity HPLC, ΠΎΠ±Π»Π°Π΄Π½Π°Π½ΠΎΠ³ΠΎ ΠΌΠ°Ρ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΎΠΌ Agilent 6120.ΠΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π° ΡΠ°ΡΡΠΈΠ½Π°. ΠΠ»Ρ ΡΠΈΠ½ΡΠ΅Π·Ρ Π°Π»ΠΊΡΠ»-2-((5-(1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-1-ΡΠ»)ΠΌΠ΅ΡΠΈΠ»Π΅Π½-4-R-1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»-3-ΡΠ»)ΡΡΠΎ)Π΅ΡΠ°Π½(ΠΏΡΠΎΠΏΠ°Π½,Π±Π΅Π½Π·)ΡΠΌΡΠ΄Π°ΡΡΠ² ΡΠΊ Π²ΠΈΡ
ΡΠ΄Π½Ρ ΡΠ΅ΡΠΎΠ²ΠΈΠ½ΠΈ Π²ΠΈΠΊΠΎΡΠΈΡΡΠ°Π»ΠΈ 2-((5-(1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-1-ΡΠ»)ΠΌΠ΅ΡΠΈΠ»-4-R-1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»-3-ΡΠ»)ΡΡΠΎ)Π°ΡΠ΅ΡΠΎ(ΠΏΡΠΎΠΏΠ°Π½,Π±Π΅Π½Π·ΠΎ)Π½ΡΡΡΠΈΠ»ΠΈ. Π‘ΠΈΠ½ΡΠ΅Π· ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ Ρ ΡΠ΅ΡΠ΅Π΄ΠΎΠ²ΠΈΡΡ Π²ΡΠ΄ΠΏΠΎΠ²ΡΠ΄Π½ΠΎΠ³ΠΎ Π°Π±ΡΠΎΠ»ΡΡΠ½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΡΡ (ΠΏΡΠΎΠΏΠ°Π½ΠΎΠ»Ρ, Π±ΡΡΠ°Π½ΠΎΠ»Ρ, ΠΎΠΊΡΠ°Π½ΠΎΠ»Ρ Π°Π±ΠΎ Π°Π»ΡΠ»ΠΎΠ²ΠΎΠ³ΠΎ ΡΠΏΠΈΡΡΡ) Ρ ΠΏΡΠΈΡΡΡΠ½ΠΎΡΡΡ Ρ
Π»ΠΎΡΠΎΡΠΎΡΠΌΡ. ΠΠΈΡΠ½ΠΎΠ²ΠΊΠΈ. ΠΡΠ΄ ΡΠ°Ρ ΡΠΈΠ½ΡΠ΅ΡΠΈΡΠ½ΠΈΡ
ΡΠ° ΡΡΠ·ΠΈΠΊΠΎ-Ρ
ΡΠΌΡΡΠ½ΠΈΡ
Π΄ΠΎΡΠ»ΡΠ΄ΠΆΠ΅Π½Ρ ΡΠΎΠ·ΡΠΎΠ±Π»Π΅Π½Ρ ΠΏΡΠ΅ΠΏΠ°ΡΠ°ΡΠΈΠ²Π½Ρ ΠΌΠ΅ΡΠΎΠ΄ΠΈΠΊΠΈ ΡΠΈΠ½ΡΠ΅Π·Ρ Π°Π»ΠΊΡΠ»-2-((5-(1Π-ΡΠ΅ΡΡΠ°Π·ΠΎΠ»-1-ΡΠ»)ΠΌΠ΅ΡΠΈΠ»Π΅Π½-4-R-1,2,4-ΡΡΠΈΠ°Π·ΠΎΠ»-3-ΡΠ»)ΡΡΠΎ)Π΅ΡΠ°Π½(ΠΏΡΠΎΠΏΠ°Π½,Π±Π΅Π½Π·)ΡΠΌΡΠ΄Π°ΡΡΠ², Π²ΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΎ ΡΠ° ΠΎΡΡΠ°ΡΠΎΡΠ½ΠΎ ΠΏΡΠ΄ΡΠ²Π΅ΡΠ΄ΠΆΠ΅Π½ΠΎ Π±ΡΠ΄ΠΎΠ²Ρ Π²ΡΡΡ
ΡΠΈΠ½ΡΠ΅Π·ΠΎΠ²Π°Π½ΠΈΡ
ΡΠΏΠΎΠ»ΡΠΊ
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