Cissus sicyoides C. Linnaeus (Vitaceae), a Potential Exotic Pest in the Lower Rio Grande Valley, Texas

English:Cissus sicyoides C. Linnaeus, a perennial vine native to tropical Mexico, Central America, and the Caribbean, has recently been rediscovered in the Lower Rio Grande Valley,Texas. A dense population of this exotic species has been located in a brushy area along a canal network and in two adjacent citrus groves near Weslaco. This species produces a dense mantle that covers other vegetation, appears to be invasive, and may pose a potential weed problem in citrus in the Lower Rio Grande Valley. Spanish: Cissus sicyoides C. Linnaeus, una enredadera perene nativa de los trópicos de México, América Central y el Caribe, se ha redescubierto recientemente en el Bajo Valle del Río Grande,Texas. Una población densa de esta especie exótica ha sido localizada en una área de matorral a lo largo de una red de canales y en dos huertas adyacentes de cítricos cercanas a Weslaco. Esta especie produce un manto denso que cubre otra vegetación, es invasiva y puede tener el potencial de convertirse en una maleza problemática para el cultivo de cítricos en el Bajo Valle del Río Grande en Texas

CRYOGENIC UPPER STAGE SYSTEM SAFETY

NASA s Exploration Initiative will require development of many new systems or systems of systems. One specific example is that safe, affordable, and reliable upper stage systems to place cargo and crew in stable low earth orbit are urgently required. In this paper, we examine the failure history of previous upper stages with liquid oxygen (LOX)/liquid hydrogen (LH2) propulsion systems. Launch data from 1964 until midyear 2005 are analyzed and presented. This data analysis covers upper stage systems from the Ariane, Centaur, H-IIA, Saturn, and Atlas in addition to other vehicles. Upper stage propulsion system elements have the highest impact on reliability. This paper discusses failure occurrence in all aspects of the operational phases (Le., initial burn, coast, restarts, and trends in failure rates over time). In an effort to understand the likelihood of future failures in flight, we present timelines of engine system failures relevant to initial flight histories. Some evidence suggests that propulsion system failures as a result of design problems occur shortly after initial development of the propulsion system; whereas failures because of manufacturing or assembly processing errors may occur during any phase of the system builds process, This paper also explores the detectability of historical failures. Observations from this review are used to ascertain the potential for increased upper stage reliability given investments in integrated system health management. Based on a clear understanding of the failure and success history of previous efforts by multiple space hardware development groups, the paper will investigate potential improvements that can be realized through application of system safety principles

Detection of Incipient Pest Infestations on Glasshouse Crops Using Multispectral Imagery and a Common Vegetation Index

Research was conducted to evaluate the effectiveness of multispectral (conventional color and color infrared) imagery and a common vegetation index (simple ratio) for detecting incipient infestations of spider mites (Tetranychus spp.; Acari: Tetranychidae) and false spider mites (Brevipalpus spp.; Acari: Tenupalpidae) on selected crops under glasshouse conditions. Although damaging infestations of both mite species were associated with intense feeding injury which was readily detectable by visual inspection, subtle levels of foliar damage caused by the two mite species were difficult to detect by visual inspection and were not readily distinguishable from undamaged (healthy) foliage in conventional color (CC) or color infrared (CIR) imagery. In contrast, foliage exhibiting subtle levels of mite feeding injury was readily distinguished from healthy foliage in derivative imagery based on the Simple Ratio (SR), a vegetation index defined as the ratio of near-infrared to red reflectance (700-1100 nm and 600-700 nm, respectively) for each pixel in the imagery. CIR and SR images were shown to be considerably more effective than CC imagery in the initial detection and subsequent monitoring of a spider mite infestation on glasshouse cucumbers which increased from incipient to annihilative levels within a period of approximately six weeks. The rationale for and potential uses of CC, CIR, and derivative imagery based on SR and other vegetative indices for monitoring pest infestations on glasshouse crops are discussed

Detecting Stress in Glasshouse Plants Using Color Infrared Imagery: A Potential New Application for Remote Sensing

English: Studies were conducted to evaluate the effectiveness of color infrared (CIR) film for detecting physiological stress in plants located within glasshouse structures. Spectroradiometer measurements obtained within and outside of a structure constructed of polycarbonate plastic indicated no significant attenuation or disruption of visible and near-infrared radiation entering the structure. CIR photographs of cucumber seedlings (Cucumis sativus) obtained within the greenhouse were comparable in quality to those obtained outside the structure, and clearly distinguished between foliage of healthy plants and those subjected to a moderate level of nitrogen stress. In CIR imagery of a trifoliate orange tree (Poncirus trifoliata (L.) Raf.) obtained within a greenhouse constructed of yellow fiberglass panels, leaves damaged by citrus red mites (Panonychus citri [McGregor]) were distinguishable from healthy foliage, and the distribution of damaged leaves on the tree itself was clearly evident. These results suggest that remote sensing techniques which have been used successfully to monitor conventional field crops are readily extendable to the commercial glasshouse environment with certain modifications. Spanish: Se condujeron estudios para evaluar la eficacia de la película infrarroja de color (CIR) para detectar el estrés fisiológico en las plantas situadas dentro de un invernadero. Las mediciones del spectroradiómetro obtenidas dentro y fuera de un invernadero construido con plástico de policarbonato no indicaron ninguna atenuación o interrupción significativa de la radiación visible y del cercano infrarrojo que penetraba al invernadero. Las fotografías con CIR de las plantas de semillero de pepino (Cucumis sativus) obtenidas dentro del invernadero fueron comparables en calidad a aquellas obtenidas fuera de este, y distinguieron claramente entre el follaje de plantas sanas y de aquellas sometidas a un nivel moderado de estrés de nitrógeno. En imágenes de CIR de un naranjo trifoliado (Poncirus trifoliata (L.) Raf.) obtenidas dentro de un invernadero construido de paneles amarillos de fibra de vidrio, las hojas dañadas por el ácaro rojo de los cítricos (Panonychus citri [McGregor]) se distinguieron del follaje sano, y la distribución de hojas dañadas en el árbol mismo fueron claramente evidentes. Estos resultados sugieren que las técnicas de detección a distancia que se han utilizado con éxito para supervisar campos de cultivo convencionales, también pueden usarse fácilmente, con algunas modificaciones, en cultivos comerciales en invernadero

A study of the centrally produced pi0pi0pi0 channel in pp interactions at 450 GeV/c

The reaction pp -> pf (pi0pi0pi0) ps has been studied at 450 GeV/c. The pi0pi0pi0 effective mass spectrum shows clear eta(547) and pi2(1670) signals. Branching ratios for the eta(547) and pi_2(1670) are given as well as upper limits for the decays of the omega(782), a1(1260) and a2(1320) into 3pi0.Comment: 10 pages, Latex, 4 Figure

JACK trial protocol: a phase III multicentre cluster randomised controlled trial of a school-based relationship and sexuality education intervention focusing on young male perspectives.

INTRODUCTION: Teenage pregnancy remains a worldwide health concern which is an outcome of, and contributor to, health inequalities. The need for gender-aware interventions with a focus on males in addressing teenage pregnancy has been highlighted as a global health need by WHO and identified in systematic reviews of (relationship and sexuality education (RSE)). This study aims to test the effectiveness of an interactive film-based RSE intervention, which draws explicit attention to the role of males in preventing an unintended pregnancy by reducing unprotected heterosexual teenage sex among males and females under age 16 years. METHODS AND ANALYSIS: A phase III cluster randomised trial with embedded process and economic evaluations. If I Were Jack encompasses a culturally sensitive interactive film, classroom materials, a teacher-trainer session and parent animations and will be delivered to replace some of the usual RSE for the target age group in schools in the intervention group. Schools in the control group will not receive the intervention and will continue with usual RSE. Participants will not be blinded to allocation. Schools are the unit of randomisation stratified per country and socioeconomic status. We aim to recruit 66 UK schools (24 in Northern Ireland; 14 in each of England, Scotland and Wales), including approximately 7900 pupils. A questionnaire will be administered at baseline and at 12-14 months postintervention. The primary outcome is reported unprotected sex, a surrogate measure associated with unintended teenage pregnancy. Secondary outcomes include knowledge, attitudes, skills and intentions relating to avoiding teenage pregnancy in addition to frequency of engagement in sexual intercourse, contraception use and diagnosis of sexually transmitted infections. ETHICS AND DISSEMINATION: Ethical approval was obtained from Queen's University Belfast. Results will be published in peer-reviewed journals and disseminated to stakeholders. Funding is from the National Institute for Health Research. TRIAL REGISTRATION NUMBER: ISRCTN99459996

Femtosecond To Millisecond Dynamics Of Light Induced Allostery In The Avena Sativa LOV Domain

The rational engineering of photosensor proteins underpins the field of optogenetics, in which light is used for spatio-temporal control of cell signalling. Optogenetic elements function by converting electronic excitation of an embedded chromophore into structural changes on the microseconds to seconds timescale, which then modulate the activity of output domains responsible for biological signalling. Using time resolved vibrational spectroscopy coupled with isotope labelling we have mapped the structural evolution of the LOV2 domain of the flavin binding phototropin Avena sativa (AsLOV2) over 10 decades of time, reporting structural dynamics between 100 femtoseconds and one millisecond after optical excitation. The transient vibrational spectra contain contributions from both the flavin chromophore and the surrounding protein matrix. These contributions are resolved and assigned through the study of four different isotopically labelled samples. High signal-to-noise data permit the detailed analysis of kinetics associated with the light activated structural evolution. A pathway for the photocycle consistent with the data is proposed. The earliest events occur in the flavin binding pocket, where a sub-picosecond perturbation of the protein matrix occurs. In this perturbed environment the previously characterised reaction between triplet state isoalloxazine and an adjacent cysteine leads to formation of the adduct state; this step is shown to exhibit dispersive kinetics. This reaction promotes coupling of the optical excitation to successive time-dependent structural changes, initially in the -sheet then -helix regions of the AsLOV2 domain, which ultimately gives rise to J-helix unfolding, yielding the signalling state. This model is tested through point mutagenesis, elucidating in particular the key mediating role played by Q513

Photoactivation of the BLUF protein PixD Probed by the Site-Specific Incorporation of Fluorotyrosine Residues

The flavin chromophore in blue light using FAD (BLUF) photoreceptors is surrounded by a hydrogen bond network that senses and responds to changes in the electronic structure of the flavin on the ultrafast time scale. The hydrogen bond network includes a strictly conserved Tyr residue, and previously we explored the role of this residue, Y21, in the photoactivation mechanism of the BLUF protein AppA by the introduction of fluorotyrosine (F-Tyr) analogs that modulated the pKa and reduction potential of Y21 by 3.5 pH units and 200 mV, respectively. Although little impact on the forward (dark to light adapted form) photoreaction was observed, the change in Y21 pKa led to a 4,000-fold increase in the rate of dark state recovery. In the present work we have extended these studies to the BLUF protein PixD, where, in contrast to AppA, modulation in the Tyr (Y8) pKa has a profound impact on the forward photoreaction. In particular, a decrease in Y8 pKa by 2 or more pH units prevents formation of a stable light state, consistent with a photoactivation mechanism that involves proton transfer or proton coupled electron transfer from Y8 to the electronically excited FAD. Conversely, the effect of pKa on the rate of dark recovery is markedly reduced in PixD. These observations highlight very significant differences between the photocycles of PixD and AppA, despite their sharing highly conserved FAD binding architectures

Luminosity-Metallicity Relation for dIrr Galaxies in the Near-Infrared

(abridged) The present work is a first step to collect homogeneous abundances and near-infrared (NIR) luminosities for a sample of dwarf irregular (dIrr) galaxies, located in nearby groups. The use of NIR luminosities is intended to provide a better proxy to mass than the blue luminosities commonly used in the literature; in addition, selecting group members reduces the impact of uncertain distances. Accurate abundances are derived to assess the galaxy metallicity. Optical spectra are collected for Hii regions in the dIrrs, allowing the determination of oxygen abundances by means of the temperature-sensitive method. For each dIrr galaxy H-band imaging is performed and the total magnitudes are measured via surface photometry. This high-quality database allows us to build a well-defined luminosity-metallicity relation in the range -13 >= M(H) >= -20. The scatter around its linear fit is reduced to 0.11 dex, the lowest of all relations currently available. There might exist a difference between the relation for dIrrs and the relation for giant galaxies, although a firm conclusion should await direct abundance determinations for a significant sample of massive galaxies. This new dataset provides an improved luminosity-metallicity relation, based on a standard NIR band, for dwarf star-forming galaxies. The relation can now be compared with some confidence to the predictions of models of galaxy evolution. Exciting follow-ups of this work are (a) exploring groups with higher densities, (b) exploring nearby galaxy clusters to probe environmental effects on the luminosity-metallicity relation, and (c) deriving direct oxygen abundances in the central regions of star-forming giant galaxies, to settle the question of a possible dichotomy between the chemical evolution of dwarfs and that of massive galaxies.Comment: 23 pages, 10 figures, accepted by A&

Genetic Studies of a Cluster of Acute Lymphoblastic Leukemia Cases in Churchill County, Nevada

OBJECTIVE: In a study to identify exposures associated with 15 cases of childhood leukemia, we found levels of tungsten, arsenic, and dichlorodiphenyldichloroethylene in participants to be higher than mean values reported in the National Report on Human Exposure to Environmental Chemicals. Because case and comparison families had similar levels of these contaminants, we conducted genetic studies to identify gene polymorphisms that might have made case children more susceptible than comparison children to effects of the exposures. DESIGN: We compared case with comparison children to determine whether differences existed in the frequency of polymorphic genes, including genes that code for enzymes in the folate and purine pathways. We also included discovery of polymorphic forms of genes that code for enzymes that are inhibited by tungsten: xanthine dehydrogenase, sulfite oxidase (SUOX gene), and aldehyde oxidase. PARTICIPANTS: Eleven case children were age- and sex-matched with 42 community comparison children for genetic analyses. Twenty parents of case children also contributed to the analyses. RESULTS: One bilalleleic gene locus in SUOX was significantly associated with either case or comparison status, depending on which alleles the child carried (without adjusting for multiple comparisons). CONCLUSIONS: Although genetic studies did not provide evidence that a common agent or genetic susceptibility factor caused the leukemias, the association between a SUOX gene locus and disease status in the presence of high tungsten and arsenic levels warrants further investigation. RELEVANCE: Although analyses of community clusters of cancer have rarely identified causes, these findings have generated hypotheses to be tested in subsequent studies