Challenges for the comprehensive management of cloud services in a PaaS framework

The 4CaaSt project aims at developing a PaaS framework that enables flexible definition, marketing, deployment and management of Cloud-based services and applications. The major innovations proposed by 4CaaSt are the blueprint and its lifecycle management, a one stop shop for Cloud services and a PaaS level resource management featuring elasticity. 4CaaSt also provides a portfolio of ready to use Cloud native services and Cloud-aware immigrant technologies

Statistical results for solar energetic electron spectra observed over 12 yr with STEREO/SEPT

This work presents a statistical analysis of near-relativistic solar energetic electron event spectra near 1 au. We use measurements of the Solar Electron and Proton Telescope (SEPT) on board STEREO in the energy range of 45–425 keV and utilize the SEPT electron event list containing all electron events observed by STEREO A and STEREO B from 2007 through 2018. We select 781 events with significant signal-to-noise ratios for our analysis and fit the spectra with single or broken-power-law functions of energy. We find 437 events showing broken power laws and 344 events only showing a single power law in the energy range of SEPT. For those events with broken power laws, we find a mean break energy of about 120 keV. We analyze the dependence of the spectral index on the rise times and peak intensities of the events as well as on the presence of relativistic electrons. The results show a relation between the power law spectral index and the rise times of the events with softer spectra belonging to rather impulsive events. Long rise-time events are associated with hard spectra as well as with the presence of higher-energy (>0.7 MeV) electrons. This group of events cannot be explained by a pure flare scenario but suggests an additional acceleration mechanism, involving a prolonged acceleration and/or injection of the particles. A dependence of the spectral index on the longitudinal separation from the parent solar source region was not found. A statistical analysis of the spectral indices during impulsively rising events where the rise times are below 20 minutes is also shown.Agencia Estatal de Investigació

Comparative gene expression profiling between human cultured myotubes and skeletal muscle tissue

<p>Abstract</p> <p>Background</p> <p>A high-sensitivity DNA microarray platform requiring nanograms of RNA input facilitates the application of transcriptome analysis to individual skeletal muscle (SM) tissue samples. Culturing myotubes from SM-biopsies enables investigating transcriptional defects and assaying therapeutic strategies. This study compares the transcriptome of aneurally cultured human SM cells versus that of tissue biopsies.</p> <p>Results</p> <p>We used the Illumina expression BeadChips to determine the transcriptomic differences between tissue and cultured SM samples from five individuals. Changes in the expression of several genes were confirmed by QuantiGene Plex assay or reverse transcription real-time PCR. In cultured myotubes compared to the tissue, 1216 genes were regulated: 583 down and 633 up. Gene ontology analysis showed that downregulated genes were mainly associated with cytoplasm, particularly mitochondria, and involved in metabolism and the muscle-system/contraction process. Upregulated genes were predominantly related to cytoplasm, endoplasmic reticulum, and extracellular matrix. The most significantly regulated pathway was mitochondrial dysfunction. Apoptosis genes were also modulated. Among the most downregulated genes detected in this study were genes encoding metabolic proteins AMPD1, PYGM, CPT1B and UCP3, muscle-system proteins TMOD4, MYBPC1, MYOZ1 and XIRP2, the proteolytic CAPN3 and the myogenic regulator MYF6. Coordinated reduced expression of five members of the GIMAP gene family, which form a cluster on chromosome 7, was shown, and the GIMAP4-reduction was validated. Within the most upregulated group were genes encoding senescence/apoptosis-related proteins CDKN1A and KIAA1199 and potential regulatory factors HIF1A, TOP2A and CCDC80.</p> <p>Conclusions</p> <p>Cultured muscle cells display reductive metabolic and muscle-system transcriptome adaptations as observed in muscle atrophy and they activate tissue-remodeling and senescence/apoptosis processes.</p

The m6A RNA demethylase ALKBH9B plays a critical role for vascular movement of Alfalfa mosaic virus in Arabidopsis

[EN] The N-6-methyladenosine (m(6)A) pathway has been widely described as a viral regulatory mechanism in animals. We previously reported that the capsid protein (CP) of alfalfa mosaic virus (AMV) interacts with the Arabidopsis m(6)A demethylase ALKBH9B regulating m(6)A abundance on viral RNAs (vRNAs) and systemic invasion of floral stems. Here, we analyze the involvement of other ALKBH9 proteins in AMV infection and we carry out a detailed evaluation of the infection restraint observed in alkbh9b mutant plants. Thus, via viral titer quantification experiments and in situ hybridization assays, we define the viral cycle steps that are altered by the absence of the m(6)A demethylase ALKBH9B in Arabidopsis. We found that ALKBH9A and ALKBH9C do not regulate the AMV cycle, so ALKBH9B activity seems to be highly specific. We also define that not only systemic movement is affected by the absence of the demethylase, but also early stages of viral infection. Moreover, our findings suggest that viral upload into the phloem could be blocked in alkbh9b plants. Overall, our results point to ALKBH9B as a possible new component of phloem transport, at least for AMV, and as a potential target to obtain virus resistance crops.This research was funded by the Spanish Agencia Estatal de Investigacion (AEI), grant numbers PID2020-115571RB-I00 to VP, and PGC2018-093445-B-I00 to JLM. MM-P was recipient of Predoctoral Contract FPI-2015-072406 from the Subprograma Formacion de Personal InvestigadorMinisterio de Economia y Competitividad (FPI-MINECO). LA-M was recipient of a Predoctoral contract from the Ministerio de Ciencia, Tecnologia y Telecomunicaciones from Costa Rica (MICITT-PINN-CON-624-2019). RN was recipient of the GRISOLIAP/2016/131 Predoctoral Contract from the Generalitat Valenciana.Martínez-Pérez, M.; Gómez Mena, MC.; Alvarado-Marchena, L.; Nadi, R.; Micol, JL.; Pallás Benet, V.; Aparicio Herrero, F. (2021). The m6A RNA demethylase ALKBH9B plays a critical role for vascular movement of Alfalfa mosaic virus in Arabidopsis. Frontiers in Microbiology. 12:1-13. https://doi.org/10.3389/fmicb.2021.745576S1131

Structural brain correlates of dementia in Huntington's disease

Altres ajuts: The present study was partially funded by the "Human Biology Project Grant" of the Huntington's Disease Society of America (USA).Huntington's disease (HD) is a fatal genetic neurodegenerative disorder with no effective treatment currently available. Progressive basal ganglia and whole-brain atrophy and concurrent cognitive deterioration are prototypical aspects of HD. However, the specific patterns of brain atrophy underlying cognitive impairment of different severity in HD are poorly understood. The aim of this study was to investigate the specific structural brain correlates of major cognitive deficits in HD and to explore its association with neuropsychological indicators. Thirty-five symptomatic early-to-mild HD patients and 15 healthy controls (HC) with available T1-MRI imaging were included in this study. In this cross-sectional study, HD patients were classified as patients with (HD-Dem) and without (HD-ND) major cognitive impairment in the range of dementia. This classification was based on previously validated PD-CRS cutoff scores for HD. Differences in brain atrophy across groups were studied by means of grey-matter volume voxel-based morphometry (GMV-VBM) and cortical thickness (Cth). Voxelwise and vertexwise general linear models were used to assess the group comparisons, controlling for the effects of age, sex, education, CAG repeat length and severity of motor symptoms. Clusters surviving p < 0.05 and family-wise error (FWE) correction were considered statistically significant. In order to characterize the impact on cognitive performance of the observed brain differences across groups, GMV and Cth values in the set of significant regions were computed and correlated with specific neuropsychological tests. All groups had similar sociodemographic profiles, and the HD groups did not significantly differ in terms of CAG repeat length. Compared to HC, both HD groups exhibited significant atrophy in multiple subcortical and parietal brain regions. However, compared to HC and HD-ND groups, HD-Dem patients showed a more prominent pattern of reduced GMV and cortical thinning. Importantly, this thinning was restricted to regions of the parietal-temporal and occipital cortices. Furthermore, these brain alterations were further associated with poorer cognitive performance in tasks assessing frontal-executive and attention domains as well as memory, language and constructional abilities. Major cognitive impairment in the range of dementia in HD is associated with brain and cognitive alterations exceeding the prototypical frontal-executive deficits commonly recognized in HD. The observed posterior-cortical damage identified by MRI and its association with memory, language, and visuoconstructive dysfunction suggest a strong involvement of extra-striatal atrophy in the onset of severe cognitive dysfunction in HD patients. Critically, major cognitive impairment in this sample was not associated with CAG repeat length, age or education. This finding could support a possible involvement of additional neuropathological mechanisms aggravating cognitive deterioration in HD

Molybdenum release from high burnup spent nuclear fuel at alkaline and hyperalkaline pH

This work presents experimental data and modelling of the release of Mo from high-burnup spent nuclear fuel (63 MWd/kgU) at two different pH values, 8.4 and 13.2 in air. The release of Mo from SF to the solution is around two orders of magnitude higher at pH = 13.2 than at pH = 8.4. The high Mo release at high pH would indicate that Mo would not be congruently released with uranium and would have an important contribution to the Instant Release Fraction, with a value of 5.3%. Parallel experiments with pure non irradiated Mo(s) and XPS determinations indicated that the faster dissolution at pH = 13.2 could be the consequence of the higher releases from metallic Mo in the fuel through a surface complexation mechanism promoted by the OH− and the oxidation of the metal to Mo(VI) via the formation of intermediate Mo(IV) and Mo(V) species