Methylation status of the Runx2 p2 promoter in a family with ectopic maxillary canines

Genetic factors contribute to the aetiology of the ectopic canine (EC) (OMIM 189490), the inheritance pattern being autosomal dominant with variable expression and incomplete penetrance. However there is also evidence for an epigenetic component. The Runx2 gene is intimately involved in the mechanism of tooth eruption. It controls differentiation and maturation of osteoblasts and is expressed in the dental follicle and alveolar bone at all stages of development [2,3]. Mutations of this gene result in delayed and ectopic eruption of teeth. There is a large CpG island spanning its proximal promoter, first exon and part of the first intron. Similarities between the inheritance patterns of EC and other methylation disorders raises the hypothesis that differential methylation of the Runx2 promoter may contribute to EC. This study investigates the methylation status of the Runx2 P2 promoter in ectopic canine subjects and unaffected controls. There was no evidence of differential methylation between the groups.peer-reviewe

Faecal haemoglobin and faecal calprotectin as indicators of bowel disease in patients presenting to primary care with bowel symptoms

OBJECTIVE: In primary care, assessing which patients with bowel symptoms harbour significant disease (cancer, higher-risk adenoma or IBD) is difficult. We studied the diagnostic accuracies of faecal haemoglobin (FHb) and faecal calprotectin (FC) in a cohort of symptomatic patients. DESIGN: From October 2013 to March 2014, general practitioners were prompted to request FHb and FC when referring patients with bowel symptoms to secondary care. Faecal samples were analysed for haemoglobin (EIKEN OC-Sensor io) and calprotectin (BÜHLMANN Calprotectin ELISA). Patients triaged to endoscopy were investigated within 6 weeks. All clinicians and endoscopists were blind to the faecal test results. The diagnostic accuracies of FHb and FC for identification of significant bowel disease were assessed. RESULTS: 1043 patients returned samples. FHb was detectable in 57.6% (median 0.4 µg/g, 95% CI 0.4 to 0.8; range 0–200). FC at 50 µg/g or above was present in 60.0%. 755 patients (54.6% women, median age 64 years (range 16–90, IQR 52–73)) returned samples and completed colonic investigations. 103 patients had significant bowel disease; the negative predictive values of FHb for colorectal cancer, higher-risk adenoma and IBD were 100%, 97.8% and 98.4%, respectively. Using cut-offs of detectable FHb and/or 200 µg/g FC detected two further cases of IBD, one higher-risk adenoma and no additional cancers. CONCLUSIONS: In primary care, undetectable FHb is a good ‘rule-out’ test for significant bowel disease and could guide who requires investigation

Landfill site trees: Potential source or sink of greenhouse gases?

Tree stems can transport greenhouse gases (GHGs) produced belowground to the atmosphere. Previous studies in natural wetland and upland ecosystems have quantified tree stem fluxes of methane (CH4), carbon dioxide (CO2) and nitrous oxide (N2O). However, tree stem GHG fluxes have not previously been measured in the context of managed environments. The work presented in this thesis aimed to quantify GHG fluxes from tree stems on closed landfill sites. To investigate the potential for trees growing on closed landfill sites to act as conduits for GHGs produced belowground to the atmosphere, GHG fluxes were measured from tree stem and soil surfaces. In situ measurements from a closed landfill site in the UK were examined for spatial and temporal patterns and evaluated against data from a comparable non-landfill area. Measurements were also conducted from landfill sites in the UK with varying management practices and different tree species present. The resulting flux values were scaled up to estimate the magnitude of tree stem GHG fluxes from closed landfills at a national level. The findings presented here show evidence of tree mediated GHG transport on closed landfill sites and temporal variations in fluxes from tree stems were also observed, with generally higher fluxes in the summer months. Stem CH4 fluxes varied between trees growing on landfill sites with different management practices. Additionally, stem N2O fluxes displayed spatial patterns, with decreasing emissions at increased height from the forest floor, indicating an underground source. Evidence suggested that GHG fluxes from closed landfills are influenced by factors including the quantity of GHG produced in the waste (linked to the age of the site), the susceptibility of the area to waterlogging and landfill management techniques put in place upon closure (for example, clay caps, cover soils and gas extraction). Upscaled CH4 and N2O flux values from tree stems on closed landfill sites corresponded to less than 1% of the total CH4 and N2O emissions reported from UK landfills in 2020. Overall, results indicated that measuring soil fluxes alone from forested landfill sites would result in an underestimation of the total surface fluxes. However, the emission rates from tree stems on closed landfills observed in this thesis do not exceed those in natural ecosystems. Therefore, with careful planning and management, the recommendation is that trees can be planted on closed landfill sites in the UK without emitting atypical levels of GHGs. However, including gas fluxes from tree stems on closed landfills would increase the accuracy of GHG budgets at national and global levels

Adding value to laboratory medicine: a professional responsibility

Laboratory medicine is a medical specialty at the centre of healthcare. When used optimally laboratory medicine generates knowledge that can facilitate patient safety, improve patient outcomes, shorten patient journeys and lead to more cost-effective healthcare. Optimal use of laboratory medicine relies on dynamic and authoritative leadership outside as well as inside the laboratory. The first responsibility of the head of a clinical laboratory is to ensure the provision of a high quality service across a wide range of parameters culminating in laboratory accreditation against an international standard, such as ISO 15189. From that essential baseline the leadership of laboratory medicine at local, national and international level needs to ‘add value’ to ensure the optimal delivery, use, development and evaluation of the services provided for individuals and for groups of patients. A convenient tool to illustrate added value is use of the mnemonic ‘SCIENCE’. This tool allows added value to be considered in seven domains: standardisation and harmonisation; clinical effectiveness; innovation; evidence-based practice; novel applications; cost-effectiveness; and education of others. The assessment of added value in laboratory medicine may be considered against a framework that comprises three dimensions: operational efficiency; patient management; and patient behaviours. The profession and the patient will benefit from sharing examples of adding value to laboratory medicine

Training manual for teaching working with Pacific students: engaging Pacific learners

The Centre’s vision is to “Embrace Pacific Cultures to enhance student experience through improvement of student engagement and achievement so that students become valued leaders and contributors to Pacific communities and New Zealand society”. The main function of the centre revolves firstly, around providing academic and pastoral support for Pacific students. Secondly, through the provision of advise and support for academic lecturers. Finally, through engagement and working closely with Pacific families and communities. The Nakuita, which is Fijian for octopus was developed by the Pacific Centre’s Director, Linda Aumua in 2008, to inform the work of the centre. The octopus has one central body which metaphorically refers to the centre and its tentacles as the Pacific support provided through the learning development lecturers infiltrating the departments and services. The concept started out with the centre employing learning development lecturers. The learning development lecturers are nurtured at the centre before they are placed in the departments, usually in the staff member’s area of expertise. The idea is that the staff member will be able to provide content support and learning development support for Pacific students. The flexibility of the octopus framework enabled the centre to collaborate with departments to co-employ the learning development lecturers with the aim of the departments picking up full employment of the Pacific staff. The success of the Nakuita has extended into the area of staff support. This include providing professional development workshops on understanding Pacific learners; engaging Pacific learners; providing input into curriculum development around embedding Pacific dimensions into the curricula and collaborating with staff on Pacific research. Through collaboration with the two lecturers in the Graduate Diploma in Not-For-Profit Management, Sandy Thompson and Fraser McDonald we are also able to produce this training resource. This collaboration started with the centre providing support for the Pacific students on their programme. This has extended to a co-employment of a Pacific staff member to provide focus support for students in this programme. This manual is divided into five sections. Section 1 is the introduction and includes a triangulation of a literature review on engagement of Pacific learners, students’ evaluation and tutors learning experiences. Section 2 draws on the experiences of the above Unitec staff and looks at meaningful ways to engage Pacific learners in classroom discussions and class content. Section 3 provides some example of teaching plans and Section 4 consist of a list of references used in this work. Finally, Section 5 is a resource/information section that will provide additional information and resources to support the work of teachers

ICD-9 Codes and Surveillance for Clostridium difficile–associated Disease

We conducted a retrospective cohort study to compare Clostridium difficile–associated disease rates determined by C. difficile–toxin assays and International Classification of Diseases, 9th Revision (ICD-9) codes. The correlation between toxin assay results and ICD-9 codes was good (κ = 0.72, p<0.01). The sensitivity of the ICD-9 codes was 78% and the specificity was 99.7%

A review of research methods in entrepreneurship 1985-2013.

Purpose – The purpose of this paper is to address the research questions: which methodologies and data gathering methods are employed by researchers publishing in top entrepreneurship journals, and how has this changed over time? Design/methodology/approach – The data gathering methods of research published in five top entrepreneurship journals between 1985 and 2013, a period of nearly three decades, were recorded. Findings – The data demonstrate that entrepreneurship research is dominated by positivist approaches and data gathering methods, but that this picture is changing over time. The data also reveal differences in methods used in research published in North American and European journals. Research limitations/implications – It is argued that increased discussion of the limitations, benefits and implications of research methods is needed across the field as a whole. It is concluded that although there is some methodological reflexivity in the field of a macro, abstract nature, there is little at the micro level of individual research designs. Originality/value – There is a number of existing reviews of methods in the field but none over such a long time period that include such a large corpus of papers. Of particular value to scholars engaged in debates about the proportions and merits of different research methods is the identification of long-term trends away from primary data gathering in general and survey approaches in particular. Debates surrounding the existence of different regional “schools” of entrepreneurship will be informed by the differing patterns of methods found in the five outlets included in the study