SEWAGE SLUDGE COMPOST ASSOCIATED TO FREQUENCY OF IRRIGATION FOR Peltophorum dubium (Sprengel) Taubert SEEDLINGS PRODUCTION

Inappropriate disposal of sewage sludge has become an environmental problem. In some Brazilian states, such as São Paulo, in natura sewage sludge use is practically prohibited due to restrictive requirements by CONAMA Resolution No. 375/2006. Therefore, a very promising alternative to reuse this residue is to compost it with other materials and use it as a substrate in the production of seedlings. The objective of this study was to evaluate the development of the seedlings of Peltophorum dubium in compost with sewage sludge, to determine which frequency of irrigation would be more adequate according to the physical characteristics of each substrate, and if this would influence the morphological and nutritional variables of the seedlings. Two composts were produced: sewage sludge composted with sugarcane bagasse and sewage sludge composted with eucalyptus bark, both in 1: 1 proportion. Commercial substrate was used for control. This study was completely randomized in a 3x3 factorial design, which three substrates and three frequencies of irrigation were tested: two, three and four times per day. The evaluated parameters were: plant height, stem diameter ratio, shoot / root ratio, leaf dry mass, root dry mass, total dry mass, green color index, Dickson quality index (IQD), water loss through leaching and substrate water retain capacity. The best results were observed in the seedlings produced in sewage sludge compound with eucalyptus bark submitted to the frequencies of two or three times a day

Produção de mudas de Ceiba speciosa em diferentes volumes de tubetes utilizando o biossólido como substrato

One of the problems encountered in the formation of stands aimed at forest restoration is the quality of the seedlings. The objective of this study was to evaluate the different volumes of tubes for the seedling production of Ceiba speciosa, using biosolids as a substrate. The experiment consisted of the seedlings production stage and evaluation of the performance in the field. The treatments were the tubes of 55, 110, 180 and 280 cm³. Both phases of this experiment were installed in a completely randomized design, with the stage of seedling production composed by four repetitions of 12 plants, and field stage composed by 20 seedlings from each treatment, with height and diameter closer to the average for the last assessment carried out in the nursery. Using biosolids as the main component of the substrate for the production of Ceiba speciosa seedlings, and for the site conditions where the forest stand was implanted, it is recommended to use the 110 cm3 tube, because in this tube occurred growth similar to plants in the tubes of larger volumes, resulting in lower volume of substrate, smaller area occupied in the seed-plot and greater ease of transport and handling.Um dos problemas encontrados na formação de povoamentos visando à restauração florestal é a qualidade das mudas. O objetivo deste trabalho foi avaliar diferentes volumes de tubetes para a produção de mudas de Ceiba speciosa, utilizando o biossólido como substrato. O experimento constou da fase de produção das mudas e da avaliação do desempenho dessas no campo. Os tratamentos utilizados foram os tubetes de 55, 110, 180 e 280 cm³. Ambas as fases do experimento foram instaladas em delineamento inteiramente casualizado, sendo a fase de viveiro composta por quatro repetições de 12 plantas, e a fase de campo composta por 20 mudas de cada tratamento, com altura e diâmetro mais próximos da média da última avaliação realizada no viveiro. Utilizando-se o biossólido como principal componente do substrato para a produção das mudas de Ceiba speciosa, e para as condições de sítio em que foi implantado o povoamento florestal, recomenda-se o uso do tubete de 110 cm3, pois neste ocorreu crescimento similar ao das plantas nos tubetes de maior volume, resultando em menor volume de substrato, menor área ocupada no viveiro e maior facilidade de transporte e manejo

BIOSSÓLIDO NA COMPOSIÇÃO DE SUBSTRATOS PARA PRODUÇÃO DE MUDAS DE DUAS ESPÉCIES FLORESTAIS UTILIZADAS NA ARBORIZAÇÃO URBANA

O lodo de esgoto é um produto resultante do tratamento de águas residuárias das Estações de Tratamento de Esgoto (ETE) que depois de estabilizado é denominado biossólido. Este componente constitui fonte de matéria orgânica e de nutrientes, apresentando potencial para a produção de mudas florestais, além de reduzir os impactos ambientais ocasionados pela disposição inadequada desse resíduo. Este trabalho objetivou avaliar o potencial do biossólido como componente de substrato para a produção de mudas de Handroanthus impetiginosus e Libidibia ferrea para fins de arborização urbana. Foram testadas diferentes proporções volumétricas de biossólido (BIO) misturado em subsolo argiloso (SA) e tratamento controle constituído por esterco bovino (EB) misturado em subsolo argiloso, constituindo as seguintes formulações: T1= 40% EB + 60% SA; T2= 20% BIO + 80% SA; T3= 40% BIO + 60% SA; T4= 80% BIO + 20%SA. Dados de altura e diâmetro de coleto foram coletados, trimestralmente, até 12 meses após o transplantio das mudas para as latas com capacidade volumétrica de 18 litros. As mudas das duas espécies não apresentaram diferenças significativas entre os tratamentos utilizados, sendo possível empregar tanto o esterco bovino quanto as dosagens de biossólido avaliadas no presente estudo

Venenos e toxinas ofídicas purificadas como ferramenta biotecnológica para o controle de Ralstonia solanacearum

The objective of this work was to evaluate the in vitro antibacterial activity of snake venoms and purified toxins on the phytopathogenic bacterium Ralstonia solanacearum. The evaluations were performed with 17 crude venoms (13 from Bothrops, 3 from Crotalus, and 1 from Lachesis) and seven toxins (1 from Bothrops and 6 from Crotalus). Antibacterial activity was assessed in MB1 medium containing solubilized treatments (1 μL mL‑1). A total of 100 μL bacterial suspension (8.4 x 109 CFU mL-1) was used. After incubation at 28°C, the number of bacterial colonies at 24, 48, and 72 hours after inoculation was evaluated. SDS-PAGE gel at 15% was used to analyze the protein patterns of the samples, using 5 μg protein of each sample in the assay. Furthermore, the minimum inhibitory concentration (MIC) and lethal concentration (LC50) values were determined by the Probit method. Venoms and toxins were able to reduce more than 90% of R. solanacearum growth. These results were either equivalent to those of the positive control chloramphenicol or even better. While MIC values ranged from 4.0 to 271.5 µg mL-1, LC50 ranged from 28.5 µg mL-1 to 4.38 mg mL-1. Ten crude venoms (7 from Bothrops and 3 from Crotalus) and two purified toxins (gyroxin and crotamine) are promising approaches to control the phytopathogenic bacterium R. solanacearum.O objetivo deste trabalho foi avaliar a atividade antibacteriana in vitro de venenos e toxinas purificadas de serpentes sobre a bactéria fitopatogênica Ralstonia solanacearum. As avaliações foram realizadas em 17 venenos brutos (13 de Bothrops, 3 de Crotalus e 1 de Lachesis) e sete toxinas (1 de Bothrops e 6 de Crotalus). A atividade antibacteriana foi avaliada em meio MB1 que continha os tratamentos solubilizados (1 μL mL-1). Utilizou-se o total de 100 μL de suspensão bacteriana (8,4 x 109 UFC mL-1). Após incubação a 28°C, avaliou-se o número de colônias bacterianas às 24, 48 e 72 horas após a inoculação. O gel SDS-PAGE a 15% foi usado para analisar o perfil proteico das amostras, tendo-se utilizado 5 μg de proteína no ensaio. Além disso, os valores de concentração inibitória mínima (CIM) e concentração letal (CL50) foram determinados pelo método Probit. Os venenos e as toxinas foram capazes de reduzir mais de 90% do crescimento de R. solanacearum. Esses resultados foram ou equivalentes aos do controle positivo cloranfenicol ou até melhores. Enquanto os valores de CIM variaram de 4,0 a 271,5 µg mL-1, a CL50 variou de 28,5 µg mL-1 a 4,38 mg mL-1. Dez venenos brutos (7 de Bothrops e 3 de Crotalus) e duas toxinas (giroxina e crotamina) são abordagens promissoras para o controle da bactéria fitopatogênica R. solanacearum

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Composted sewage sludge with sugarcane bagasse as a commercial substrate for Eucalyptus urograndis seedling production

Sewage sludge can be used as a source of organic matter and nutrients, whereas sugarcane bagasse can be used as a decompaction material; by composting a mixture of the two, a low-cost substrate for forest nurseries can be obtained. This research investigated the use of composted sewage sludge with sugarcane bagasse (CSB) as a commercial substrate in nurseries to grow seedlings of the hybrid clone Eucalyptus urograndis. Several CSB treatments were evaluated in comparison with a control (no P addition) and a commercial substrate (CS). Before composting, CSB was conditioned with P to increase its final concentration: CSB+1.5, 3.0, and 4.5% triple superphosphate (TP) or reactive phosphate (RP). After 120 d, the Eucalyptus response to all eight substrates was assessed by: i) plant morphological traits (H, height; D, diameter; SB, shoot biomass; RB, root biomass; TB, total dry biomass; GCI, green color intensity; and root system quality) and ii) chemical parameters of shoots and roots. Significant differences among treatments were ascertained using an ANOVA, and variability was interpreted using principal factor analysis (PFA). The treatment with CSB+3% TP (TP3.0) exhibited statistically (p < 0.05) higher performance in regards to morphological parameters (H, D, SB, TB) and the nutrient contents of shoots and roots (N, P, Ca, Na, Mn, Zn, and Cu) than the other treatments and control. The results suggest that B and K could play a fundamental role in both the observed variability and the improved plant performance in the TP3.0 substrate. PFA also showed i) the key role of OM as the primary source/sink of some pivotal macronutrients/heavy metals and ii) the existence of important antagonistic/synergistic effects between elements as a primary driver affecting the concentration/behavior of elements in the shoot/root system. Overall, the research demonstrated that with an addition of only 3.0% TP, the CSB performance was better than the most commonly used and widespread commercial substrate in industrial forest nurseries