INTERACTIVE ENVIRONMENTAL PROJECT

Jako umělci*kyně máme velkou výsadu v tom, že se s osobními, společenskými i jinými tématy, které nás zrovna pálí, můžeme vyrovnávat tvůrčím způsobem. Tato diplomová práce se potýká s tématem transformace. Proměna prostoru, těla či stavu volá po odpovědi v podobě bezpečného stabilního prostoru, který tyto zásahy obrazně reflektuje. Vzniká unikátní enviroment, který divákům*čkám poskytuje nedotknutelné útočiště podobně jako to umí mateřské lůno. V této práci jsem měla příležitost propojit více tvůrčích přístupů a vytvořit tak dílo, které vychází z mé dosavadní umělecké zkušenosti a posouvá ji zase o krok dál.ObhájenoAs artists we have the privilege to tackle personal, social or other topics that resonate with us through creative expression. This diploma work focuses on the theme of transformation. The transformation of space, of one's body shape or state of being calls for an answers in the form of a safe and stable space that visually reflects these interferences. Here we have a unique environment, providing an untouchable hideout, similar to a mother's womb. Through this work I had the chance to connect various art forms and make something based on my previous art experiences and move forward as an artist

Enhancing cisplatin anticancer effectivity and migrastatic potential by modulation of molecular weight of oxidized dextran carrier

The molecular weight (Mw) of dextran derivatives, such as regioselectively oxidized dicarboxydextran (DXA), is greatly influencing their faith in an organism, which could be possibly used to improve anticancer drug delivery. Here we present a modified method of sulfonation-induced chain scission allowing direct and accurate control over the Mw of DXA without increasing its polydispersity. Prepared DXA derivatives (Mw = 10–185 kDa) have been conjugated to cisplatin and the Mw of the carrier found to have a significant impact on cisplatin release rates, in vitro cytotoxicity, and migrastatic potential. Conjugates with the high-Mw DXA showed particularly increased anticancer efficacy. The best conjugate was four times more effective against malignant prostatic cell lines than free cisplatin and significantly inhibited the ovarian cancer cell migration. This was traced to the characteristics of spontaneously formed cisplatin-crosslinked DXA nanogels influenced by Mw of DXA and amount of loaded cisplatin. © 2021 Elsevier LtdMinistry of Education, Youth, and Sports of the Czech Republic -DKRVO [RP/CPS/2020/006]; Ministry of Education, Youth and Sports of the Czech RepublicMinistry of Education, Youth & Sports - Czech Republic [CZ.02.1.01/0.0/0.0/15_003/0000444, MUNI/A/1698/2020, MUNI/A/1246/2020, LM2018127]; Masaryk University in Brno [InGA/SUP/08/2020]; Czech Science FoundationGrant Agency of the Czech Republic [19-16861S]; Charles University in Prague [Progress Q26/LF1, Q27/LF1]RP/CPS/2020/006; Univerzita Karlova v Praze, UK: Q27/LF1; Ministerstvo Školství, Mládeže a Tělovýchovy, MŠMT: CZ.02.1.01/0.0/0.0/15_003/0000444, InGA/SUP/08/2020, MUNI/A/1246/2020, MUNI/A/1698/2020; Grantová Agentura České Republiky, GA ČR: 19-16861S, LM201812

Modulation of Induced Cytotoxicity of Doxorubicin by Using Apoferritin and Liposomal Cages

Doxorubicin is an effective chemotherapeutic drug, however, its toxicity is a significant limitation in therapy. Encapsulation of doxorubicin inside liposomes or ferritin cages decreases cardiotoxicity while maintaining anticancer potency. We synthesized novel apoferritin-and liposome-encapsulated forms of doxorubicin ("Apodox" and "lip-8-dox") and compared its toxicity with doxorubicin and Myocet on prostate cell lines. Three different prostatic cell lines PNT1A, 22Rv1, and LNCaP were chosen. The toxicity of the modified doxorubicin forms was compared to conventional doxorubicin using the MTT assay, real-time cell impedance-based cell growth method (RTCA), and flow cytometry. The efficiency of doxorubicin entrapment was 56% in apoferritin cages and 42% in the liposome carrier. The accuracy of the RTCA system was verified by flow-cytometric analysis of cell viability. The doxorubicin half maximal inhibition concentrations (IC50) were determined as 170.5, 234.0, and 169.0 nM for PNT1A, 22Rv1, and LNCaP, respectively by RTCA. Lip8-dox is less toxic on the non-tumor cell line PNT1A compared to doxorubicin, while still maintaining the toxicity to tumorous cell lines similar to doxorubicin or epirubicin (IC50 = 2076.7 nM for PNT1A vs. 935.3 and 729.0 nM for 22Rv1 and LNCaP). Apodox IC50 was determined as follows: 603.1, 1344.2, and 931.2 nM for PNT1A, 22Rv1, and LNCaP

Návrh nového systému organizace a řízení akciové společnosti Výzkumný ústav oděvní Prostějov

PrezenčníNeuvedenoNeuveden

Modulation of doxorubicin-induced toxicity using nanocarriers

Charles University in Prague Faculty of Science Abstract Name and surname: Mgr. Michaela Fojtů Name of the thesis: Modulation of doxorubicin-induced toxicity using nanocarriers In the clinical practice anthracycline antibiotic doxorubicin is a very potent and extensively prescribed chemotherapeutic agent. It is widely utilized in the therapy of variety haematological malignancies and solid tumors. Nonetheless, its administration is usually accompanied with several serve side effects. The most serious one is development of dose-dependent and cumulative cardiotoxicity which can be manifested even years after chemotherapy. Here were show that encapsulation of doxorubicin into nanocarriers represented in this study by apoferritin cages, or liposomal vesicles may help to overcome these limitations while simultaneously maintain the anticancer efficiency of the drug. Moreover, loading of chemotherapeutics inside the nanocarriers cavity, or binding of these drugs on their surface appeared as an effective approach offering solutions for many limitations associated with current cancer treatment, prolonging the drug circulation half-life, or increasing the accumulation of chemotherapeutics in the tumor tissue

Book-Object

Tato bakalářská práce se zaměřuje na využívání netradičních materiálů v rámci mého oboru. Mou snahou bylo experimentovat s ovocem a zeleninou a tvořit z nich vizuálně zajímavé listy, které dohromady budou tvořit něco, čemu se dá říkat jedlá kniha. V této práci popisuji, jak jsem k tomuto tématu došla, co mě ovlivnilo při tvorbě díla samotného a jaké nové poznatky jsem získala. Práce nastiňuje moji filosofii tvorby a má mírný enviromentální přesah.ObhájenoThis bachelors work is focused on exploring new materials whitin my field of study. My intention was to experiment with fruits and vegetables and to create visually interesting organic sheets which will together form something so called edible book. In this work I am trying to describe what inspired me to focus on this topic and what were the influences in the process of making the artwork itself. I am also describing what was the outcome of the whole procedure. The thesis outlines my philosophy of creation and has a slight environmental overlap

INTERACTIVE ENVIRONMENTAL PROJECT

Jako umělci*kyně máme velkou výsadu v tom, že se s osobními, společenskými i jinými tématy, které nás zrovna pálí, můžeme vyrovnávat tvůrčím způsobem. Tato diplomová práce se potýká s tématem transformace. Proměna prostoru, těla či stavu volá po odpovědi v podobě bezpečného stabilního prostoru, který tyto zásahy obrazně reflektuje. Vzniká unikátní enviroment, který divákům*čkám poskytuje nedotknutelné útočiště podobně jako to umí mateřské lůno. V této práci jsem měla příležitost propojit více tvůrčích přístupů a vytvořit tak dílo, které vychází z mé dosavadní umělecké zkušenosti a posouvá ji zase o krok dál.ObhájenoAs artists we have the privilege to tackle personal, social or other topics that resonate with us through creative expression. This diploma work focuses on the theme of transformation. The transformation of space, of one's body shape or state of being calls for an answers in the form of a safe and stable space that visually reflects these interferences. Here we have a unique environment, providing an untouchable hideout, similar to a mother's womb. Through this work I had the chance to connect various art forms and make something based on my previous art experiences and move forward as an artist

Modulation of doxorubicin-induced toxicity using nanocarriers

Charles University in Prague Faculty of Science Abstract Name and surname: Mgr. Michaela Fojtů Name of the thesis: Modulation of doxorubicin-induced toxicity using nanocarriers In the clinical practice anthracycline antibiotic doxorubicin is a very potent and extensively prescribed chemotherapeutic agent. It is widely utilized in the therapy of variety haematological malignancies and solid tumors. Nonetheless, its administration is usually accompanied with several serve side effects. The most serious one is development of dose-dependent and cumulative cardiotoxicity which can be manifested even years after chemotherapy. Here were show that encapsulation of doxorubicin into nanocarriers represented in this study by apoferritin cages, or liposomal vesicles may help to overcome these limitations while simultaneously maintain the anticancer efficiency of the drug. Moreover, loading of chemotherapeutics inside the nanocarriers cavity, or binding of these drugs on their surface appeared as an effective approach offering solutions for many limitations associated with current cancer treatment, prolonging the drug circulation half-life, or increasing the accumulation of chemotherapeutics in the tumor tissue.Univerzita Karlova v Praze Přírodovědecká fakulta Abstrakt Jméno a příjmení: Mgr. Michaela Fojtů Název práce: Modulace doxorubicinem indukované toxicity prostřednictvím nanonosičů Antracyklinové antibiotikum doxorubicin představuje velmi účinné a v klinické praxi ve velké míře předepisované chemoterapeutikum. Je hojně užíván k léčbě hematologických malignit i řady solidních nádorů. Administraci doxorubicinu nicméně často doprovází řada vedlejších účinků. Tím nejzávažnějším je rozvoj dávkově dependentní kumulativní kardiotoxicity, která se může projevit i několik let po ukončení chemoterapie. V této práci jsme ukázali, že enkapsulace doxorubicinu do nanonosičů jako jsou apoferitinové klece, či lipozomální vezikuly může pomoci těmto limitacím předcházet, a to za současného zachování protinádorového účinku léčiva. Uzavírání chemoterapeutik do nanonosičů, či vazba těchto léčiv na jejich povrch se ukázala být užitečným přístupem nabízející redukci hned několika problémů doprovázejících současnou léčbu maligních onemocnění, a to jak díky cílenému transportu do specifické tkáně, či prodlužování biologického poločasu léčiva v cirkulaci, tak také díky zvýšené akumulaci chemoterapeutika v nádorové tkáni.Katedra biochemieDepartment of BiochemistryPřírodovědecká fakultaFaculty of Scienc

Graphene oxide nanoplatelets potentiate anticancer effect of cisplatin in human lung cancer cells

Graphene oxide (GO) has been widely explored by many in drug delivery strategies and toxicity assays. The toxicity of graphene oxide depends on the size of the sheets. Smaller sheets show lower toxicity, a quality which is essential for utilization in biomedical applications. However, despite vast research on GO, anticancer properties and drug carrier capabilities of graphene oxide nanoplatelets have yet to be fully explored. Herein, we have uniquely prepared graphene oxide nanoplatelets (GONPs) from well-defined stacked graphite nanofibers (SGNF) with a base of 50 × 50 nm2 for toxicity and drug potentiation studies when coadministered with the chemotherapeutic drug cisplatin (CP) in human lung cancer cells, A549 cells. Results obtained from our studies have found that not only were GONPs able to act as drug carriers, but they can also significantly potentiate anticancer effect of CP in A549 cells.Agency for Science, Technology and Research (A*STAR)National Research Foundation (NRF)This work was supported by the project Advanced Functional Nanorobots (reg. No. CZ.02.1.01/0.0/0.0/15_003/0000444 financed by the EFRR). Authors thank the A*Star grant (No. SERC A1783c0005), Singapore. This research is supported by the National Research Foundation, Prime Minister’s Office, Singapore under its CREATE programme. This work was supported by Grant Agency of the Czech Republic (GACR - 18-24089S)

Selectively oxidized cellulose with adjustable molecular weight for controlled release of platinum anticancer drugs

The synthesis of selectively oxidized cellulose, 2,3-dicarboxycellulose (DCC), is optimized for preparation of highly oxidized material for biological applications, which includes control over the molecular weight of the product during its synthesis. Conjugates of DCC and cisplatin simultaneously offer a very high drug binding efficiency (>90%) and drug loading capacity (up to 50 wt %), while retaining good aqueous solubility. The adjustable molecular weight of the DCC together with variances in drug feeding ratio allows to optimize cisplatin release profiles from delayed (<2% of cisplatin released during 6 h) to classical burst release with more than 60% of cisplatin released after 24 h. The release rates are also pH-dependent (up to 2 times faster release at pH 5.5 than at pH 7.4), which allows to exploit the acidic nature of tumor microenvironment. Extensive in vitro studies were performed on eight different cell lines for two cisplatin-DCC conjugates with different release profiles. In comparison with free cisplatin, both cisplatin-DCC conjugates demonstrated considerably lower cytotoxicity toward healthy cells. Conjugates with burst release profiles were found more effective against prostate cell lines, while DCC conjugates with slower release were more cytotoxic against ovarian and lung carcinoma cell lines. In vivo studies indicated a significantly longer survival rate, a reduction in tumor volume, and a higher accumulation of platinum in tumors of mice treated with the cisplatin-DCC conjugate in comparison to those treated by free cisplatin. © 2019 American Chemical Society.Czech Science Foundation [16-05961S]; Ministry of Education, Youth and Sports of the Czech Republic Program NPU I [LO1504]; Ministry of Education, Youth and Sports of the Czech Republic [2018 MUNI/A/1255/2018, MUNI/A/1553/2018]; MEYS [LM2015043]; NM