Yield, quality and conservation of heterozygous tomatoes in alcobaça, nonripening and ripening inhibitor loci

O objetivo deste trabalho foi avaliar os atributos de produtividade, qualidade e conservação pós-colheita de tomates, para comparar os efeitos promovidos pelos alelos alcobaça (norA), nonripening (nor) e ripening inhibitor (rin) em heterozigose, isoladamente ou em duplas combinações, sobre frutos de tomateiros híbridos. Foram avaliados dez tratamentos: sete híbridos experimentais quase-isogênicos, com background FloraDade x Tropic de genótipos nor+/norA, rin+/rin, nor+/nor, nor/norA, nor+/norA rin+/rin e nor+/nor rin+/rin; e três testemunhas comerciais (Floradade, Tropic e Carmen F1). Contrariamente aos genótipos rin+/rin e nor+/nor, o genótipo nor+/norA não prolongou, significativamente, a firmeza dos frutos em pós-colheita. Os genótipos duplo-mutantes norA/nor, nor+/norA rin+/rin e nor+/nor rin+/rin foram eficientes em atrasar a perda de firmeza e a evolução da coloração dos frutos; os efeitos dos locos nor+/norA e rin+/rin, juntos, sofreram desvios significativos em relação à soma dos efeitos desses locos, quando atuaram separadamente, no sentido de intensificarem esses atrasos. O uso de híbridos heterozigotos, nas duplas combinações entre os locos norA, nor e rin, mostrou-se vantajoso por propiciar frutos firmes, com maior extensão da vida pós-colheita, em comparação com o uso dos híbridos portadores desses locos isoladamente. A qualidade dos frutos duplo-mutantes não foi limitada pelo atraso na evolução da coloração vermelha.Thie objective of this work was to evaluate yield, quality and postharvest conservation of tomatoes, to compare the effects promoted by the alcobaça (norA), nonripening (nor) and ripening inhibitor (rin) alleles, in heterozygosis, singly or in double combinations on hybrid tomato fruits. Ten treatments were evaluated: seven experimental nearly isogenic hybrids of FloraDade x Tropic background or reciprocal with nor+/norA, rin+/rin, nor+/nor, nor/norA, nor+/norA rin+/rin and nor+/nor rin+/rin genotypes, and three commercial checks (FloraDade, Tropic and Carmen F1). Oppositely to the genotypes rin+/rin and nor+/nor, the genotype nor+/norA did not significantly extend the firmness of the fruits in postharvest. The double mutant genotypes norA/nor, nor+/norA rin+/rin and nor+/nor rin+/rin were efficient in delaying firmness loss and the evolution of fruits colouring; the effects of loci nor+/norA and rin+/rin, together, underwent significant deviations relatively to the sum of these loci effects, when acting singly, in the sense of enhancing those delays. Use of heterozygous hybrids in the double combinations among loci norA, nor and rin proved advantageous for providing firm fruits with a longer postharvest lifetime, as compared with the hybrids carrying these loci singly. The quality of double mutant fruits was not restricted by the delay in the evolution of red coloration

Generalidades sobre o quadro clínico da Rinossinusite: uma revisão narrativa de literatura: Generalities about the clinical picture of Rhinosinusitis: a narrative literature reviewv

A rinossinusite é um processo inflamatório da mucosa dos seios paranasais e da cavidade nasal. O sistema nasossinusal é responsável pelo balanço adequado entre a fabricação e o clearence de muco nas cavidades paranasais. A fisiologia deste é de vital importância para a proteção das vias aéreas superiores. No advém, determinados fatores podem acarretar um desbalanço nesse complexo, consequentemente um processo inflamatório. Qualquer fator que altere a drenagem, seja por obstrução, maior produção ou espessamento do muco, como processo infecciosos ou alérgicos, haverá uma impactação de secreções e a facilitação de colonização bacteriana, dando início ao processo infeccioso. A identificação da inflamação do nariz e seios paranasais é basicamente clínica. A suspeição desta ocorre através da manifestação de dois ou mais sintomatologias. As quais são o bloqueio ou obstrução nasal, a descarga nasal, pressão ou dor facial e redução ou perda do olfato. De modo geral, é essencial à prevenção básica das rinossinusites agudas é barrar a infecção viral. O suporte inclui medidas gerais de higiene, alimentação e hidratação, imunização para o combate de vírus respiratórios , administração de fármacos para turbinar o sistema imune se necessário

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear un derstanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5–7 vast areas of the tropics remain understudied.8–11 In the American tropics, Amazonia stands out as the world’s most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepre sented in biodiversity databases.13–15 To worsen this situation, human-induced modifications16,17 may elim inate pieces of the Amazon’s biodiversity puzzle before we can use them to understand how ecological com munities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple or ganism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region’s vulnerability to environmental change. 15%–18% of the most ne glected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lostinfo:eu-repo/semantics/publishedVersio

ATLANTIC EPIPHYTES: a data set of vascular and non-vascular epiphyte plants and lichens from the Atlantic Forest

Epiphytes are hyper-diverse and one of the frequently undervalued life forms in plant surveys and biodiversity inventories. Epiphytes of the Atlantic Forest, one of the most endangered ecosystems in the world, have high endemism and radiated recently in the Pliocene. We aimed to (1) compile an extensive Atlantic Forest data set on vascular, non-vascular plants (including hemiepiphytes), and lichen epiphyte species occurrence and abundance; (2) describe the epiphyte distribution in the Atlantic Forest, in order to indicate future sampling efforts. Our work presents the first epiphyte data set with information on abundance and occurrence of epiphyte phorophyte species. All data compiled here come from three main sources provided by the authors: published sources (comprising peer-reviewed articles, books, and theses), unpublished data, and herbarium data. We compiled a data set composed of 2,095 species, from 89,270 holo/hemiepiphyte records, in the Atlantic Forest of Brazil, Argentina, Paraguay, and Uruguay, recorded from 1824 to early 2018. Most of the records were from qualitative data (occurrence only, 88%), well distributed throughout the Atlantic Forest. For quantitative records, the most common sampling method was individual trees (71%), followed by plot sampling (19%), and transect sampling (10%). Angiosperms (81%) were the most frequently registered group, and Bromeliaceae and Orchidaceae were the families with the greatest number of records (27,272 and 21,945, respectively). Ferns and Lycophytes presented fewer records than Angiosperms, and Polypodiaceae were the most recorded family, and more concentrated in the Southern and Southeastern regions. Data on non-vascular plants and lichens were scarce, with a few disjunct records concentrated in the Northeastern region of the Atlantic Forest. For all non-vascular plant records, Lejeuneaceae, a family of liverworts, was the most recorded family. We hope that our effort to organize scattered epiphyte data help advance the knowledge of epiphyte ecology, as well as our understanding of macroecological and biogeographical patterns in the Atlantic Forest. No copyright restrictions are associated with the data set. Please cite this Ecology Data Paper if the data are used in publication and teaching events. © 2019 The Authors. Ecology © 2019 The Ecological Society of Americ

Pervasive gaps in Amazonian ecological research

Biodiversity loss is one of the main challenges of our time,1,2 and attempts to address it require a clear understanding of how ecological communities respond to environmental change across time and space.3,4 While the increasing availability of global databases on ecological communities has advanced our knowledge of biodiversity sensitivity to environmental changes,5,6,7 vast areas of the tropics remain understudied.8,9,10,11 In the American tropics, Amazonia stands out as the world's most diverse rainforest and the primary source of Neotropical biodiversity,12 but it remains among the least known forests in America and is often underrepresented in biodiversity databases.13,14,15 To worsen this situation, human-induced modifications16,17 may eliminate pieces of the Amazon's biodiversity puzzle before we can use them to understand how ecological communities are responding. To increase generalization and applicability of biodiversity knowledge,18,19 it is thus crucial to reduce biases in ecological research, particularly in regions projected to face the most pronounced environmental changes. We integrate ecological community metadata of 7,694 sampling sites for multiple organism groups in a machine learning model framework to map the research probability across the Brazilian Amazonia, while identifying the region's vulnerability to environmental change. 15%–18% of the most neglected areas in ecological research are expected to experience severe climate or land use changes by 2050. This means that unless we take immediate action, we will not be able to establish their current status, much less monitor how it is changing and what is being lost

Genetic variability in the 5' UTR and NS5A regions of hepatitis C virus RNA isolated from non-responding and responding patients with chronic HCV genotype 1 infection

Submitted by Nuzia Santos ([email protected]) on 2013-07-03T18:27:33Z No. of bitstreams: 1 8. ARAUJO FMG, SONODA IV.pdf: 390202 bytes, checksum: 12579764f3ec1d032cea575fc9365b80 (MD5)Made available in DSpace on 2013-07-03T18:27:33Z (GMT). No. of bitstreams: 1 8. ARAUJO FMG, SONODA IV.pdf: 390202 bytes, checksum: 12579764f3ec1d032cea575fc9365b80 (MD5) Previous issue date: 2008Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilHospital das Clínicas. Instituto Alfa de GastroenterologiaUniversidade Federal de Minas Gerais. Instituto de Ciencias Biológicas. Laboratório de Biodiversidade e Evolução Molecular Departamento de Biologia Geral. Belo Horizonte, MG, BrasilSanta Casa de Belo Horizonte. Programa de Pós-Graduação e Pesquisa. Belo Horizonte, MG, BrasilSequence variation among different hepatitis C virus (HCV) isolates has adaptive significance and reflects the modes and intensities of selection mechanisms operating on the virus. In this work, we sought to investigate using classical population genetics parameters, the genetic variability of HCV genotype 1 using the 5' UTR and NS5A regions from treatment non-responding and responding groups of patients. Both regions showed low genetic varia-bility and the 5' UTR showed neutral deviation. No differences were observed in the nonsynonymous/synonymous nucleotide substitution ratio among groups for NS5A. The analysis of molecular variance test of the 5' UTR region showed an 11.94% variation among groups. Phylogenetic analysis showed no correlation between sequence variations and therapeutic response