Interactions of sodium transport, cell volume, and calcium in frog urinary bladder

The volume of individual cells in intact frog urinary bladders was determined by quantitative microscopy and changes in volume were used to monitor the movement of solute across the basolateral membrane. When exposed to a serosal hyposmotic solution, the cells swell as expected for an osmometer, but then regulate their volume back to near control in a process that involves the loss of KCl. We show here that volume regulation is abolished by Ba++, which suggests that KCl movements are mediated by conductive channels for both ions. Volume regulation is also inhibited by removing Ca++ from the serosal perfusate, which suggests that the channels are activated by this cation. Previously, amiloride was observed to inhibit volume regulation: in this study, amiloride-inhibited, hyposmotically swollen cells lost volume when the Ca++ ionophore A23187 was added to Ca++-replete media. We attempted to effect volume changes under isosmotic conditions by suddenly inhibiting Na+ entry across the apical membrane with amiloride, or Na+ exit across the basolateral membrane with ouabain. Neither of these Na+ transport inhibitors produced the expected results. Amiloride, instead of causing a decrease in cell volume, had no effect, and ouabain, instead of causing cell swelling, caused cell shrinkage. However, increasing cell Ca++ with A23187, in both the absence and presence of amiloride, caused cells to lose volume, and Ca++-free Ringer's solution (serosal perfusate only) caused ouabain-blocked cells to swell. Finally, again under isosmotic conditions, removal of Na+ from the serosal perfusate caused a loss of volume from cells exposed to amiloride. These results strongly suggest that intracellular Ca++ mediates cell volume regulation by exerting a negative control on apical membrane Na+ permeability and a positive control on basolateral membrane K+ permeability. They also are compatible with the existence of a basolateral Na+/Ca++ exchanger

Evidence-Based Practice Integration Across Hawaiʻi’s Academic Institutions

A podium presentation given at presented at Leadership in Action: Leading from Within, a conference sponsored by the American Organization of Nursing Executives-Hawaiʻi Chapter, the Hawaiʻi State Center for Nursing, and the American College of Healthcare Executives Hawaiʻi-Pacific Chapter on November 3, 2017 in Honolulu, Hawaiʻi.Learner Objective 1: Describe the creation of a state-wide evidence-based program to integrate EBP into undergraduate curricula Learner Objective 2: Delineate specific approaches identified by educators to facilitate academic integration BACKGROUND: The Institute of Medicine (2010), the Quality and Safety Education in Nursing Institute (2014), and the American Association of Colleges of Nursing (2006) consistently charge nurse academicians to integrate evidence-based practice (EBP) content into their nursing programs. However, sporadic and narrowly-focused content often fails to achieve EBP competencies necessary for the novice practicing nurse. METHODS: A PubMed search from 2011 to 2016 using keywords ‘Teaching EBP AND Nurs’ yielded 47 articles; 22 were critiqued and graded. Most (12) used program evaluation designs; nine reported strategies used to teach critiquing skills. The evidence base for how best to integrate EBP content into undergraduate curriculum is lacking in quality, quantity, and consistency. However, previously tested pedagogical strategies were suggested, such as integrating EBP content into each course, moving from simple to complex concepts, and application to practice. RESULTS: The Hawai‘i State Center for Nursing offered a three-day EBP workshop for nurse educators from schools of nursing, statewide. Using the Iowa Model as the framework, nurse faculty leveled and spiraled EBP content across undergraduate curriculum. With the assistance of a research librarian and EBP faculty, educators learned information literacy content and identified various teaching strategies for each course, entry level to senior year. Fifty nurse faculty from seven of the nine state’s schools of nursing attended the workshop. IMPLICATIONS FOR NURSING: The Center’s statewide approach offers the academic community the support necessary for successful integration of EBP. Systematic evaluation will be important for future implementation

Sodium transport effects on the basolateral membrane in toad urinary bladder

In toad urinary bladder epithelium, inhibition of Na transport with amiloride causes a decrease in the apical (Vmc) and basolateral (Vcs) membrane potentials. In addition to increasing apical membrane resistance (Ra), amiloride also causes an increase in basolateral membrane resistance (Rb), with a time course such that Ra/Rb does not change for 1-2 min. At longer times after amiloride (3-4 min), Ra/Rb rises from its control values to its amiloride steady state values through a secondary decrease in Rb. Analysis of an equivalent electrical circuit of the epithelium shows that the depolarization of Vcs is due to a decrease in basolateral electromotive force (Vb). To see of the changes in Vcs and Rb are correlated with a decrease in Na transport, external current (Ie) was used to clamp Vmc to zero, and the effects of amiloride on the portion of Ie that takes the transcellular pathway were determined. In these studies, Vcs also depolarized, which suggests that the decrease in Vb was due to a decrease in the current output of a rheogenic Na pump. Thus, the basolateral membrane does not behave like an ohmic resistor. In contrast, when transport is inhibited during basolateral membrane voltage clamping, the apical membrane voltage changes are those predicted for a simple, passive (i.e., ohmic) element

Local and regional ecological morphology of dung beetle assemblages across four biogeographic regions

Aim Niche partitioning within species assemblages is thought to influence species packing and/or total niche space occupied. The evolution of dung beetles (Scarabaeinae) is likely to have been strongly influenced by inter-specific competition, leading to niche partitioning. We consider whether local-scale processes leave a signature in regional patterns of functional diversity in dung beetle assemblages, and investigate the correlation between total exploited ecomorphological space and density of species packing with increased species richness. We test whether ecomorphological space occupied by local assemblages reflects that of their regional species pool, and the extent to which ecomorphological space is convergent or divergent within functional groups across regional pools. Location Neotropics, Africa, Australia and Madagascar. Methods Dung beetle assemblages were collected in a standardized manner from four biogeographic regions. Ecomorphological similarity among the assemblages was assessed by multivariate analysis of 19 linear measurements for 300 species and three functional nesting types (roller, tunneller or dweller), firstly on a local level within the Neotropics and Afrotropics, and then between the regional species pools. Results Key body measurements, in particular the hind tibia, separated rollers and tunnellers into largely non-overlapping entities along the first three axes of the shape analysis. Three Neotropical assemblages, which vary widely in species numbers, each harboured a similar amount of morphometric variation, resulting in increasingly dense species packing with greater species richness. Similar findings were obtained in two South African assemblages. Assemblages in the four biogeographic regions showed largely similar distributions of ecomorphological variation, including the separation of rollers and tunnellers, despite their distant phylogenetic relationships. Ecomorphological similarity among regions was particularly high in tunnellers, whilst the rollers exhibited greater regional differentiation. Main conclusions Local assemblages evidently represent the full diversity of functional groups available in the regional pool, even in species-poor assemblages. There is a strong trend towards convergence in morphology separating tunnellers and rollers in phylogenetically independent lineages. The ecomorphological similarity of regional assemblages suggests that morphological convergence is the result of common selective forces active within the assemblages themselves. This lends support to the widely hypothesized effect of inter-specific interactions and niche partitioning in determining assemblage composition and lineage evolution in the Scarabaeinae. © 2011 Blackwell Publishing Ltd

Spectroscopy of Luminous Compact Blue Galaxies in Distant Clusters I. Spectroscopic Data

We used the DEIMOS spectrograph on the Keck II Telescope to obtain spectra of galaxies in the fields of five distant, rich galaxy clusters over the redshift range 0.5 < z < 0.9 in a search for luminous, compact, blue galaxies (LCBGs). Unlike traditional studies of galaxy clusters, we preferentially targeted blue cluster members identified via multi-band photometric pre-selection based on imaging data from the WIYN telescope. Of the 1288 sources that we targeted, we determined secure spectroscopic redshifts for 848 sources, yielding a total success rate of 66%. Our redshift measurements are in good agreement with those previously reported in the literature, except for 11 targets which we believe were previously in error. Within our sample, we confirm the presence of 53 LCBGs in the five galaxy clusters. The clusters all stand out as distinct peaks in the redshift distribution of LCBGs with the average number density of LCBGs ranging from 1.65+-0.25 Mpc^-3 at z=0.55 to 3.13+-0.65 Mpc^-3 at z=0.8. The number density of LCBGs in clustes exceeds the field desnity by a factor of 749+-116 at z=0.55; at z=0.8, the corresponding ratio is E=416+-95. At z=0.55, this enhancement is well above that seen for blue galaxies or the overall cluster population, indicating that LCBGs are preferentially triggered in high-density environments at intermediate redshifts.Comment: 45 pages, 19 figures, accepted to ApJ. For Full resolution figure and data tables, see http://www.salt.ac.za/~crawford/projects/deimos

Extending gravitational wave burst searches with pulsar timing arrays

Pulsar timing arrays (PTAs) are being used to search for very low frequency gravitational waves. A gravitational wave signal appears in pulsar timing residuals through two components: one independent of and one dependent on the pulsar's distance, called the 'Earth term' (ET) and 'pulsar term' (PT), respectively. The signal of a burst (or transient) gravitational wave source in pulsars' residuals will in general have the Earth and pulsar terms separated by times of the order of the time of flight from the pulsar to the Earth. Therefore, both terms are not observable over a realistic observation span, but the ETs observed in many pulsars should be correlated. We show that pairs (or more) of pulsars can be aligned in such a way that the PTs caused by a source at certain sky locations can arrive at Earth within a time window short enough to be captured during a realistic observation span. We find that for the pulsars within the International Pulsar Timing Array (IPTA) ~67 per cent of the sky produces such alignments for pulsars terms separated by less than 10 years. We compare estimates of the source event rate that would be required to observe one signal in the IPTA if searching for the correlated ETs, or in searching via the PTs, and find that event rates would need to be about two orders of magnitude higher to observe an event with the PTs than the ETs. We also find that an array of hundreds of thousands of pulsars would be required to achieve similar numbers of observable events in PT or ET searches. This disfavours PTs being used for all-sky searches, but they could potentially be used target specific sources and be complementary to ET only searches.Comment: version accepted for MNRA

Resiliency and women exiting sex trade industry work

Summary: A qualitative approach was used to explore the experience of exiting sex trade industry work from the perspective of 19 adults formerly involved in the sex trade industry. A narrative approach to data collection was used explore the participants’ experiences of successful exiting and phenomenological analysis was employed to identify themes that reflected the ways in which participants developed resiliency throughout the exiting process. Findings: Themes include: connection (including subthemes: survivor presence, children, and spirituality), resources (including subthemes: networks, structure and safety) and personal growth. Significance: These themes represent women with diverse experiences in the sex trade industry, including a majority who were victimized by (internal) sex trafficking. The findings demonstrate opportunities for social work practice to address the diverse needs of individuals exiting the sex trade industry, specifically for those who experienced sex trafficking and sexual exploitation

Integrable Deformations of c^=1\hat{c}=1 Strings in Flux Backgrounds

We study d=2 0A string theory perturbed by tachyon momentum modes in backgrounds with non-trivial tachyon condensate and Ramond-Ramond (RR) flux. In the matrix model description, we uncover a complexified Toda lattice hierarchy constrained by a pair of novel holomorphic string equations. We solve these constraints in the classical limit for general RR flux and tachyon condensate. Due to the non-holomorphic nature of the tachyon perturbations, the transcendental equations which we derive for the string susceptibility are manifestly non-holomorphic. We explore the phase structure and critical behavior of the theory.Comment: 39 pages, 4 figure

Heterotic Strings in Two Dimensions and New Stringy Phase Transitions

We discuss heterotic string theories in two dimensions with gauge groups Spin(24) and Spin(8) x E_8. After compactification the theories exhibit a rich spectrum of states with both winding and momentum. At special points some of these stringy states become massless, leading to new first order phase transitions. For example, the thermal theories exhibit standard thermodynamics below the phase transition, but novel and peculiar behavior above it. In particular, when the radius of the Euclidean circle is smaller than the phase transition point the torus partition function is not given by the thermal trace over the spacetime Hilbert space. The full moduli space of compactified theories is 13 dimensional, when Wilson lines are included; the Spin(24) and Spin(8) x E_8 theories correspond to distinct decompactification limits.Comment: 32 pages; v2: references added, minor change