Alguns fongs de primavera interessants trobats als Pirineus catalans

Es descriuen o comenten 3 ascomicets i 4 basidiomicets trobats en boscos montans o subalpins de Catalunya, durant la primavera de 2006. Són els següents: Encoelia furfuracea, Entoloma favrei, E. sericeonitens, E. sericeum var. cinereoopacum, E. ventricosum, Ptychoverpa bohemica i Rhytisma salicinum. S'aporten dibuixos dels caràcters microscòpics i fotografies dels exemplars d'alguns dels tàxons.Three ascomycetes and four basidiomycetes found in mountain or subalpine forests of Catalonia (NE Spain) during spring of 2006 are described or discussed, namely: Encoelia furfuracea, Entoloma favrei, E. sericeonitens, E. sericeum var. cinereoopacum, E. ventricosum, Ptychoverpa bohemica and Rhytisma salicinum. Drawings of microscopic characters and photographs of sorne of the specimens are included

Contribució al conocimiento de la micoflora del Parc de la Serralada Litoral

Aportem l'examen de 6 espècies, corresponents a 3 gèneres diferents de basidiomicets, Entoloma, Leucoagaricus, i Trichocybe, mitjançant la descripció dels seus caràcters morfològics, i en algun cas, també amb anàlisis filogenètiques. També hi afegim una contribució a la iconografia de les espècies en el seu hàbitat natural, amb imatges dels caràcters microscòpics més rellevants. Es descriuen les següents espècies: Entoloma dysthales f. acystidiosum, Entoloma scabiosum, Entoloma speculum, Leucoagaricus crystallifer, Leucoagaricus ionidicolor, i Trichocybe pubèrula.We provide the examination of 6 species corresponding to 3 different genera of basidiomycetes, Entoloma, Leucoagaricus, and Trichocybe, describing their morphological characters, and, in some instances, including phylogenetic analysis. Also included in this study is a contribution to the iconography of the species in their natural habitat, with images of the most relevant microscopic characters. The following species are described: Entoloma dysthales f. acystidiosum Noordel., E. scabiosum (Fr) Quél., E. speculum (Fr) Quél. Leucoagaricus crystallifer Vellinga, L. ionidicolor Bellù & Lanzoni, and Trichocybe puberula (Kuyper) Vizzini.Aportamos el examen de 6 especies correspondientes a 3 géneros distintos de basidiomicetes, Entoloma, Leucoagaricus, y Trichocybe, mediante la descripción de sus caracteres morfológicos, y en algún caso incluyendo análisis filogenéticos. También se incluye en este estudio una aportación a la iconografía de las especies en su hábitat natural, con imágenes de los caracteres microscópicos más relevantes. Se describen las siguientes especies: Entoloma dysthales f. acystidiosum Noordel., E. scabiosum (Fr) Quél., E. speculum (Fr) Quél. Leucoagaricus crystallifer Vellinga, L. ionidicolor Bellù & Lanzoni, y Trichocybe puberula (Kuyper) Vizzini

Preliminary morphologic and molecular study of teh Entoloma rusticoides group (Agaricales - Basidiomycota)

Es presenta un estudi sobre el grup rusticoides del gènere Entoloma subgènere Claudopus a la Península Ibérica. L'anàlisi filogenètica, sobre la base de 48 seqüències d'ITS i 45 seqüències de LSU, entre les quals, les dels tipus de 8 dels tàxons analitzats, mostra una marcada diversitat genètica que concorda amb la notable variabilitat observada en aquest grup. D'acord amb aquest fet, es descriuen els següents nous tàxons: E. almeriense, E. graphitipes f. cystidiatum, E. halophilum, E. muscoalpinum i E. phaeocarpum. A més, es tipifiquen E. phaeocyathus i E. rusticoides. S'inclouen claus provisionals d'identificació. S'aporten descripcions macro i microscòpiques, dibuixos i fotografies de la majoria dels tàxons estudiats.A study of the Entoloma rusticoides-group (subgenus Claudopus) in the Iberian peninsula is presented. Phylogenetic inference, based on 48 nrITS and 45 28S nrLSU sequences, including those of 8 type specimens, revealed a high genetic diversity, in accordance with the observed morphological variability in this group. In addition new taxa are proposed to accomodate independent lineages (E. almeriense, E. graphitipes f. cystidiatum, E. halophilum, E. muscoalpinum and E. phaeocarpum) and the typification of E. phaeocyathus and E. rusticoides is proposed. Preliminary taxonomic keys are included to help identification. For most species macro and microscopic descriptions, drawings and photographs are presented

Preliminary morphologic and molecular study of teh Entoloma rusticoides group (Agaricales - Basidiomycota)

Es presenta un estudi sobre el grup rusticoides del gènere Entoloma subgènere Claudopus a la Península Ibérica. L'anàlisi filogenètica, sobre la base de 48 seqüències d'ITS i 45 seqüències de LSU, entre les quals, les dels tipus de 8 dels tàxons analitzats, mostra una marcada diversitat genètica que concorda amb la notable variabilitat observada en aquest grup. D'acord amb aquest fet, es descriuen els següents nous tàxons: E. almeriense, E. graphitipes f. cystidiatum, E. halophilum, E. muscoalpinum i E. phaeocarpum. A més, es tipifiquen E. phaeocyathus i E. rusticoides. S'inclouen claus provisionals d'identificació. S'aporten descripcions macro i microscòpiques, dibuixos i fotografies de la majoria dels tàxons estudiats.A study of the Entoloma rusticoides-group (subgenus Claudopus) in the Iberian peninsula is presented. Phylogenetic inference, based on 48 nrITS and 45 28S nrLSU sequences, including those of 8 type specimens, revealed a high genetic diversity, in accordance with the observed morphological variability in this group. In addition new taxa are proposed to accomodate independent lineages (E. almeriense, E. graphitipes f. cystidiatum, E. halophilum, E. muscoalpinum and E. phaeocarpum) and the typification of E. phaeocyathus and E. rusticoides is proposed. Preliminary taxonomic keys are included to help identification. For most species macro and microscopic descriptions, drawings and photographs are presented

Wildland–urban interface fires in spain: summary of the policy framework and recommendations for improvement

Southern Europe is recurrently being hit by forest fires affecting wildland–urban interface (WUI) areas which, particularly in the last decade, have resulted in tremendous consequences. In the years to come, self-protection of communities will be a first priority over fire suppression, demanding better fire-resistant and resilient WUI scenarios through actions grounded on solid and sound regulations and legislation. As of today, the European Union as a whole, and the Member States in particular, are belatedly articulating new and appropriate regulations and implementing policies for the protection of WUI areas against forest fires. Spain is one of the EU Member States, holding 1.1 million ha of WUI areas (above 4% of the total forested land) and experiencing an average of 12,500 forest fires per year over the past decade. In this paper a review of the state of the art on regulations, codes, plans and recommendations on WUI fire prevention and management in Spain is presented. Shortcomings due to the current lack of building and urban planning standards and technical codes for WUI communities are highlighted. We underline some paramount needs to be covered by scientific research and fire engineering in particular topics. Some of them have received little attention in the literature related to European WUI fires while some others have been almost unexplored, such as planning of low-fuel fringes, design of road networks and accessibility, dimension of water supply networks, study of ignitability and combustibility of residential vegetation, role of construction methods and materials, and the wildland–industrial interface. Outcomes from research activities on such topics should lead to appropriately drive and inform the policy making processes on WUI fire prevention and management in Spain and, by extension, in other southern European countries under a similar situation.Postprint (author's final draft

The effects of death and post-mortem cold ischemia on human tissue transcriptomes

Post-mortem tissues samples are a key resource for investigating patterns of gene expression. However, the processes triggered by death and the post-mortem interval (PMI) can significantly alter physiologically normal RNA levels. We investigate the impact of PMI on gene expression using data from multiple tissues of post-mortem donors obtained from the GTEx project. We find that many genes change expression over relatively short PMIs in a tissue-specific manner, but this potentially confounding effect in a biological analysis can be minimized by taking into account appropriate covariates. By comparing ante- and post-mortem blood samples, we identify the cascade of transcriptional events triggered by death of the organism. These events do not appear to simply reflect stochastic variation resulting from mRNA degradation, but active and ongoing regulation of transcription. Finally, we develop a model to predict the time since death from the analysis of the transcriptome of a few readily accessible tissues.Peer ReviewedPostprint (published version

Mutations in the EXT1 and EXT2 genes in Spanish patients with multiple osteochondromas

Multiple osteochondromas is an autosomal dominant skeletal disorder characterized by the formation of multiple cartilage-capped tumours. Two causal genes have been identified, EXT1 and EXT2, which account for 65% and 30% of cases, respectively. We have undertaken a mutation analysis of the EXT1 and EXT2 genes in 39 unrelated Spanish patients, most of them with moderate phenotype, and looked for genotype-phenotype correlations. We found the mutant allele in 37 patients, 29 in EXT1 and 8 in EXT2. Five of the EXT1 mutations were deletions identified by MLPA. Two cases of mosaicism were documented. We detected a lower number of exostoses in patients with missense mutation versus other kinds of mutations. In conclusion, we found a mutation in EXT1 or in EXT2 in 95% of the Spanish patients. Eighteen of the mutations were novel

p38γ is essential for cell cycle progression and liver tumorigenesis

The cell cycle is a tightly regulated process that is controlled by the conserved cyclin-dependent kinase (CDK)–cyclin protein complex1. However, control of the G0-to-G1 transition is not completely understood. Here we demonstrate that p38 MAPK gamma (p38γ) acts as a CDK-like kinase and thus cooperates with CDKs, regulating entry into the cell cycle. p38γ shares high sequence homology, inhibition sensitivity and substrate specificity with CDK family members. In mouse hepatocytes, p38γ induces proliferation after partial hepatectomy by promoting the phosphorylation of retinoblastoma tumour suppressor protein at known CDK target residues. Lack of p38γ or treatment with the p38γ inhibitor pirfenidone protects against the chemically induced formation of liver tumours. Furthermore, biopsies of human hepatocellular carcinoma show high expression of p38γ, suggesting that p38γ could be a therapeutic target in the treatment of this disease

Identification of circulating microRNA profiles associated with pulmonary function and radiologic features in survivors of SARS-CoV-2-induced ARDS

There is a limited understanding of the pathophysiology of postacute pulmonary sequelae in severe COVID-19. The aim of current study was to define the circulating microRNA (miRNA) profiles associated with pulmonary function and radiologic features in survivors of SARS-CoV-2-induced ARDS. The study included patients who developed ARDS secondary to SARS-CoV-2 infection (n = 167) and a group of infected patients who did not develop ARDS (n = 33). Patients were evaluated 3 months after hospital discharge. The follow-up included a complete pulmonary evaluation and chest computed tomography. Plasma miRNA profiling was performed using RT-qPCR. Random forest was used to construct miRNA signatures associated with lung diffusing capacity for carbon monoxide (DLCO) and total severity score (TSS). Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were conducted. DLCO < 80% predicted was observed in 81.8% of the patients. TSS showed a median [P25;P75] of 5 [2;8]. The miRNA model associated with DLCO comprised miR-17-5p, miR-27a-3p, miR-126-3p, miR-146a-5p and miR-495-3p. Concerning radiologic features, a miRNA signature composed by miR-9-5p, miR-21-5p, miR-24-3p and miR-221-3p correlated with TSS values. These associations were not observed in the non-ARDS group. KEGG pathway and GO enrichment analyses provided evidence of molecular mechanisms related not only to profibrotic or anti-inflammatory states but also to cell death, immune response, hypoxia, vascularization, coagulation and viral infection. In conclusion, diffusing capacity and radiological features in survivors from SARS-CoV-2-induced ARDS are associated with specific miRNA profiles. These findings provide novel insights into the possible molecular pathways underlying the pathogenesis of pulmonary sequelae.This work is supported by Instituto de Salud Carlos III (COV20/00110), co-funded by European Regional Development Fund (ERDF)/“A way to make Europe”. CIBERES is an initiative of the Instituto de Salud Carlos III. Suported by: Programa de donaciones “estar preparados” UNESPA (Madrid, Spain) and Fundación Francisco Soria Melguizo (Madrid, Spain). Supported by La Fundació La Marató de TV3, projecte amb codi 202108-30/-31. COVIDPONENT is funded by Institut Català de la Salut and Gestió de Serveis Sanitaris. MM is the recipient of a predoctoral fellowship (PFIS: FI21/00187) from Instituto de Salud Carlos III. MCGH is the recipient of a predoctoral fellowship from “University of Lleida”. DdGC has received financial support from Instituto de Salud Carlos III (Miguel Servet 2020: CP20/00041), co-funded by the European Social Fund (ESF)/“Investing in your future”. AC acknowledges receiving financial support from Instituto de Salud Carlos III (ISCIII; Sara Borrell 2021: CD21/00087). ENL and GL were funded by COVID1005 and ACT210085 from National Agency of Investigation & Development & Development (ANID), Chil