Berkes'in Türk bilimine katkısı

Taha Toros Arşivi, Dosya No: 172-Niyazi-Mediha Berkesİstanbul Kalkınma Ajansı (TR10/14/YEN/0033) İstanbul Development Agency (TR10/14/YEN/0033

Lysosomal oxidation of LDL alters lysosomal pH, induces senescence and increases secretion of pro-inflammatory cytokines in human macrophages

Objective We have shown that aggregated low density lipoproteins (LDL) is internalised by macrophages and oxidised in lysosomes by redox-active iron. We have now investigated if the lysosomal oxidation of LDL impairs lysosomal function and if a lysosomotropic antioxidant can prevent these alterations. Approach and Results LDL aggregated by sphingomyelinase (SMase-LDL) caused increased lysosomal lipid peroxidation in human monocyte-derived macrophages or THP-1 macrophage-like cells, as shown by a fluorescent probe, Foam-LPO. The pH of the lysosomes was increased considerably by lysosomal LDL oxidation as shown by Lysosensor Yellow/Blue and LysoTracker Red. SMase-LDL induced senescence-like properties in the cells as shown by β-galactosidase staining and levels of p53 and p21. Inflammation plays a key role in atherosclerosis. SMase-LDL treatment increased the LPS-induced secretion of TNF-α, IL-6 and MCP-1. The lysosomotropic antioxidant, cysteamine inhibited all of the above changes. Conclusions Targeting lysosomes with antioxidants, such as cysteamine, to prevent the intralysosomal oxidation of LDL might be a novel therapy for atherosclerosis

Impact of COVID-19 pandemic on mental health of general population in Kashmir Valley, India

Background: The high morbidity and morbidity associated with epidemics and disasters and economic losses thereof is a high psychosocial risk. Estimation of the burden of mental health issues and recognition of various risk groups will lead to better allocation of resources to prevent the increased burden of psychiatric morbidity during the epidemic.Methods: This was an online survey conducted during COVID-19 pandemic based on self reporting DASS 21 scale.Results: Among the participants 49.5% had depression, 34.8% had anxiety and 22.3% had stress in varying severity from mild to extremely severe forms. Female gender, less age, high level of education and unemployment were the risk factors for increased psychiatric morbidity.Conclusions: There is a dire need to recognize the mental health threat due to COCID-19 pandemic and implement the steps to protect the vulnerable population from it.

TELEVISED RELIGIOUS REALITY AND DEVELOPMENT OF SOCIAL ATTITUDES AMONG YOUTH

Among many crucial factors of personality, social attitude is considered among the prioritized ones. There are many contributors in the development of personality, however, televised religious realities seems very essential because of linkage to religion. Therefore, the research aimed to examine the relationship of televised religious realities with social attitude of youth. The methodology opted for the research was correlational. Population of the study was University students. There was total 607 respondents in the study. A self-developed questionnaire containing 16 items was developed. The analysis of the data revealed that televised religious realities correlate poorly with social attitude. Moreover, the results revealed that televised religious realities impact social attitudes poorly. The research implies that televised religious realities needs significant attention because of having relationship with social attitudes of youth

Emotional Intelligence and Innovative Work Behaviour: A Review

With the headway of globalization, the achievement and endurance of an organisation are pawned to innovation and adaptability. Innovation plays a crucial role in gaining a competitive advantage within the organization. For any organization to be innovative, employees' innovative work behavior assumes an indispensable role. Capturing innovative ideas from employees at different levels, building an active and collaborative workforce within organisation, recognizing employees’ effort, and communicating effectively with all stakeholders are the vital building blocks of innovation. Building the culture of innovation that drives productive and sustainable innovation practices can spark from within the workforce helping organizations to remain competitive and stay ahead in the long haul. Research indicates that innovation and creativity are attained through emotional intelligence. Given this contention, the purpose of this paper is to examine the role emotional intelligence plays in bringing innovative work behavior within the employees. An extensive literature review on the said constructs was conducted where both variables were studied independently and the linkages that both variables exhibit together were also studied from different database sources like Emerald insight, Science Direct, Taylor and Francis, Springer, Google Scholar, etc. and also, the models of emotional intelligence were put forth

Lysosomal oxidation of Low Density Lipoproteins

Oxidation of LDL is widely believed to be a key process in the pathogenesis of atherosclerosis. However, LDL oxidation has been shown to be inhibited by interstitial fluid and also large clinical trials have shown no protection by antioxidant. Recent work has shown that LDL can be oxidised by iron within the lysosomes of macrophages. Here, we have explored the possible mechanism by which iron is able to oxidise LDL under lysosomal conditions, and also how lysosomotropic antioxidant, cysteamine is able to prevent it. More recently, it has been shown that human macrophages are able to rapidly phagocytose LDL aggregated by enzymes, such as sphingomyelinase (SMaseLDL) and oxidised it by iron inside lysosomes, which have a pH of about 4.5. Here, the chemical characteristics (lipid hydroperoxides and oxysterols) of SMase-LDL oxidised by inorganic iron at lysosomal pH (4.5) have been determined in vitro and compared to the native LDL. In the lysosomes of macrophages, SMase-LDL increased the intralysosomal lipid peroxidation and ceroid formation which was greatly inhibited by cysteamine. There is good evidence which suggests that lysosomal dysfunction plays an important role in the atherosclerotic plaque development. Here, it is shown that lysosomal oxidation of SMase-LDL in human macrophages can cause lysosomal dysfunction, induce ceroid associated cellular senescence, and increase the expression of inflammatory cytokine like TNF-α. The work here also demonstrates that preventing the lysosomal LDL oxidation, with antioxidants like cysteamine, offers protection against the SMase-LDL induced lysosomal dysfunctio

A comparative prospective study of maternofetal outcome in advanced and younger maternal age group in higher socioeconomic strata

Background: Maternal age in pregnancy is increasing over the world and has been widely documented. Nowadays many women delay their pregnancy even up to the 40th year of life because of different reasons like changing social and economic trend. Simultaneously higher advanced technique and better supported maternal and neonatal care also exist.Methods: To have an idea of balance between advanced age and advanced support this comparative prospective study was done on 40 advanced and 40 younger maternal age groups to compare the pregnancy outcomes. To find out the association Chi-Square and unpaired ‘t’ test was used.Results: It was observed in this study that Assisted reproductive techniques (mode of conception) and cesarean section rates were significantly higher in advanced maternal age. Although there were no significant differences in antenatal and postpartum complications between the two groups.Conclusions: It can be concluded that if a women with advanced maternal age is cared at a hospital with advanced techniques, the adverse pregnancy outcomes will not be different from the non-elderly women

Genetic Diversity among Desmodium Gangeticum (L.) DL Accessions a Desmodin Synthesising Ethno-Medicinal Plant With SSR and Internal Transcribed Spacer Region for Species Conservation

In the present study simple sequence repeat (SSR) andintertranscribed spacer markers markers were used inDesmodium gangeticum to find out the genetic diversityanalysis for the biodiversity conservation of the Species as theplant is being used in medicinal preparations as well as havemany industrial applications. The study was aimed to find outthe relationships among different accessions. We find intrapopulationmean genetic value of (1.835) and Shannon (0.641)among the accessions. in AMOVA analysis, we noticed smallvariation accessions as (P = 1).The dendrogram generatedthrough distance matrix using the SIMQUAL-Dice Coefficientmodule of NTSYSpc ver matches to some extent with the PCAgraph. In the barcode ITS marker analysis the UPGMA showsthe accessions clustered into two groups. Blast results showedmaximum E-value in DG2 accession. In conclusion the abovebased markers can be used for the population conservationand authentication of the above medicinally important plant