Immune response after experimental allergic encephalomyelitis in rats subjected to calorie restriction

Male Lewis rats (6 weeks-old) were submitted to a calorie restriction equivalent to 33% or 66% of food restriction. Fifteen days later, groups of 7 animals were injected with complete Freund's adjuvant plus spinal cord homogenate (SCH) to induce experimental allergic encephalomyelitis (EAE) or with complete Freund's adjuvant alone. EAE was defined solely on clinical grounds. Rats were assessed daily for clinical signs of EAE and were killed on day 15 after immunization. Both diet and SCH injection diminished body weight significantly. In contrast to rats receiving a normal diet or a 33% calorie-restricted diet, rats subjected to severe calorie restriction did not exhibit clinical signs of EAE. Concomitantly with the lack of disease manifestation, 66% of calorie-restricted rats injected with SCH showed significantly less splenic and lymph node mitogenic response to concanavalin A (Con A) and a higher splenic response to lipopolysaccharide. Fewer splenic, lymph node and thymic CD4(+ )cells, greater numbers of splenic and lymph node CD8(+ )and CD4(+)- CD8(+ )cells, and fewer splenic T, B and T-B cells, and lymph node and thymic B and T-B cells were observed. There was impaired interferon (IFN)-γ production occurred in the three examined tissues. The results are compatible with the view that the acute phase of EAE can be curtailed by severe calorie restriction, presumably through impaired IFN-γ production

Perfil psicopatológico de pacientes con Dependencia Emocional

Diversos autores defienden la Dependencia Emocional como una categoría diagnóstica propia. El perfil psicopatológico de las personas afectadas por este trastorno ha sido expuesto a nivel teórico o basado en observaciones clínicas. Con este estudio se pretende determinar el perfil psicopatológico de una muestra de pacientes que presentaban problemas de Dependencia Emocional, desadaptativa, así como estudiar la relación entre autoestima y síntomas psicopatológicos. Se analiza el perfil sintomático a través de los resultados del Symptom CheckList-90 items-Revised (SCL-90-R) y el nivel de autoestima medido a través del Rosenberg Self-Esteem Scale (RSES) de una muestra de 33 pacientes de género femenino admitidos consecutivamente, que consultaron por problemas de dependencia emocional. Las pacientes presentaban elevada puntuación en las escalas obsesión-compulsión, sensibilidad interpersonal, depresión, ansiedad, hostilidad, ideación paranoide, psicoticismo (medidas a través del SCL-90-R) comparados con baremos normativos de población psiquiátrica española y una autoestima baja (medida a través del RSES). En la muestra la autoestima correlaciona de manera negativa con somatización, obsesión-compulsión, depresión y hostilidad. Se discuten las características y perfiles de esta muestra clínica. English abstract: Several authors defend that Affective Dependence is a diagnostic category. The psychopathological profile of these patients has been explained from a theoretical point of view or based on clinical observations. In that study we want to determinate the pathological profile of a sample of patients with problems of affective dependence, and to study the relationship between their self-esteem and their psychopathological symptoms. We analyzed the symptomatic profile of a sample of 33 female consecutive patients consulting for affective dependence, measured trough the Symptom CheckList-90 items-Revised (SCL-90-R) and the Rosenberg Self-Esteem Scale (RSES). Patients present high obsessive-compulsive, interpersonal sensitivity, depression, anxiety, hostility, paranoid ideation, psychoticism (measured by the SCL- 90-R) compared with compared with normative scores of Spanish psychiatric population, and low self-esteem (measured by the RSES). In these subjects the self- esteem showed negative correlation with somatization, obsessive-compulsive, depression and hostility. These characteristics and profiles are discussed

Electromyography: A Simple and Accessible Tool to Assess Physical Performance and Health during Hypoxia Training. A Systematic Review

Producción CientíficaHypoxia causes reduced partial pressure of oxygen in arterial blood and induces adaptations in skeletal muscle that may affect individuals’ physical performance and muscular health. These muscular changes are detectable and quantifiable by electromyography (EMG), an instrument that assesses electrical activity during active contraction at rest. EMG is a relatively simple and accessible technique for all patients, one that can show the degree of the sensory and motor functions because it provides information about the status of the peripheral nerves and muscles. The main goal of this review is to evaluate the scientific evidence of EMG as an instrument for monitoring different responses of skeletal muscles subjected to external stimuli such as hypoxia and physical activity. A structured search was conducted following the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines in Medline/PubMed, Scielo, Google Scholar, Web of Science, and Cochrane Library Plus. The search included articles published in the last 25 years until May 2020 and was restricted to English- and Spanish-language publications. As such, investigators identified nine articles that met the search criteria. The results determined that EMG was able to detect muscle fatigue from changes in the frequency spectrum. When a muscle was fatigued, high frequency components decreased and low frequency components increased. In other studies, EMG determined muscle activation increased during exercise by recruiting motor units and by increasing the intensity of muscle contractions. Finally, it was also possible to calculate the mean quadriceps quadratic activity used to obtain an image of muscle activation. In conclusion, EMG offers a suitable tool for monitoring the different skeletal muscle responses and has sufficient sensitivity to detect hypoxia-induced muscle changes produced by hypoxic stimuli. Moreover, EMG enhances an extension of physical examination and tests motor-system integrity

Electromyography: a simple and accessible tool to assess physical performance and health during hypoxia training. A systematic review

Hypoxia causes reduced partial pressure of oxygen in arterial blood and induces adaptations in skeletal muscle that may affect individuals’ physical performance and muscular health. These muscular changes are detectable and quantifiable by electromyography (EMG), an instrument that assesses electrical activity during active contraction at rest. EMG is a relatively simple and accessible technique for all patients, one that can show the degree of the sensory and motor functions because it provides information about the status of the peripheral nerves and muscles. The main goal of this review is to evaluate the scientific evidence of EMG as an instrument for monitoring different responses of skeletal muscles subjected to external stimuli such as hypoxia and physical activity. A structured search was conducted following the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines in Medline/PubMed, Scielo, Google Scholar, Web of Science, and Cochrane Library Plus. The search included articles published in the last 25 years until May 2020 and was restricted to English- and Spanish-language publications. As such, investigators identified nine articles that met the search criteria. The results determined that EMG was able to detect muscle fatigue from changes in the frequency spectrum. When a muscle was fatigued, high frequency components decreased and low frequency components increased. In other studies, EMG determined muscle activation increased during exercise by recruiting motor units and by increasing the intensity of muscle contractions. Finally, it was also possible to calculate the mean quadriceps quadratic activity used to obtain an image of muscle activation. In conclusion, EMG offers a suitable tool for monitoring the different skeletal muscle responses and has sufficient sensitivity to detect hypoxia-induced muscle changes produced by hypoxic stimuli. Moreover, EMG enhances an extension of physical examination and tests motor-system integrity

SDSS IV MaNGA: Dependence of Global and Spatially Resolved SFR-M ∗ Relations on Galaxy Properties

Indexación: Scopus.The galaxy integrated Hα star formation rate-stellar mass relation, or SFR(global)-M ∗(global) relation, is crucial for understanding star formation history and evolution of galaxies. However, many studies have dealt with SFR using unresolved measurements, which makes it difficult to separate out the contamination from other ionizing sources, such as active galactic nuclei and evolved stars. Using the integral field spectroscopic observations from SDSS-IV MaNGA, we spatially disentangle the contribution from different Hα powering sources for ∼1000 galaxies. We find that, when including regions dominated by all ionizing sources in galaxies, the spatially resolved relation between Hα surface density (ΣHα(all)) and stellar mass surface density (Σ∗(all)) progressively turns over at the high Σ∗(all) end for increasing M ∗(global) and/or bulge dominance (bulge-to-total light ratio, B/T). This in turn leads to the flattening of the integrated Hα(global)-M ∗(global) relation in the literature. By contrast, there is no noticeable flattening in both integrated Hα(H ii)-M ∗(H ii) and spatially resolved ΣHα(H ii)-Σ∗(H ii) relations when only regions where star formation dominates the ionization are considered. In other words, the flattening can be attributed to the increasing regions powered by non-star-formation sources, which generally have lower ionizing ability than star formation. An analysis of the fractional contribution of non-star-formation sources to total Hα luminosity of a galaxy suggests a decreasing role of star formation as an ionizing source toward high-mass, high-B/T galaxies and bulge regions. This result indicates that the appearance of the galaxy integrated SFR-M ∗ relation critically depends on their global properties (M ∗(global) and B/T) and relative abundances of various ionizing sources within the galaxies.http://iopscience.iop.org/article/10.3847/1538-4357/aaa9bc/met

Palaeoenvironmental analysis of the Aragonian (middle Miocene) mammalian faunas from the Madrid Basin based on body-size structure

As a consequence of the growth of the Antarctic ice-sheet during the middle Miocene, a global decrease of temperatures and an associated increase in aridity provoked several environmental changes all around the world. Such environmental variations can be detected in the continental record of the mammalian prey community structure using a synecological approach. Because of the good quality of its faunas, the rich Aragonian vertebrate fossil record from the Madrid Basin (Spain) appears as a good candidate to explore these environmental changes. In order to analyse the climatic evolution of the Iberian Peninsula associated to the Global Cooling Event, two classic palaeosynecological methodologies (cenograms and body size diversity), based on body-size community structure, were applied to 6 fossil sites from the Madrid Basin, ranging over 2 million years (15.5 – 13.5 Ma). To establish a comparative framework, we used the ecological faunal data from 100 modern localities uniformly distributed all around the world. Our palaeoenvironmental inference is based on multivariate discriminant analysis of the dataset containing both modern and fossil mammals. Finally, we can conclude that the Aragonian mammalian assemblage from the Madrid Basin showed a predominance of semiarid environments with pulses of higher aridity in biozones Dc, E and F associated with the Global Cooling Event of the middle Miocene.Como consecuencia del crecimiento del casquete polar Antártico, durante el Mioceno medio, se produjo un descenso global de la tem­peratura asociado a un aumento de la aridez. Este tipo de cambios ambientales pueden ser detectados en el registro continental, siguiendo un modelo sinecológico para analizar la estructura de las comunidades de las faunas de mamíferos herbívoros. Debido a la calidad de sus faunas, el registro fósil de las faunas de vertebrados del Aragoniense de la Cuenca de Madrid se antoja como un buen candidato para explorar estos patrones de cambio ambiental. Con el objetivo de analizar la evolución climática de la Península Ibérica asociada al evento de enfriamiento global durante este lapso temporal, dos metodologías paleosinecológicas clásicas (cenogramas y espectros de diversidad de tamaño corporal), basadas en la estructura de tamaños corporales de las comunidades de mamíferos, han sido aplicadas a la fauna de herbívoros fósiles presente en seis yacimientos de la Cuenca de Madrid, abarcando aproximadamente 2 millones de años (15.5 – 13.5 Ma). Se ha establecido un marco comparativo usando los datos ecológicos de la fauna de mamíferos presente en 100 localidades uniformemente distribuidas por todo el planeta. Esta inferencia paleoambiental está basada en la aplicación de análisis discriminante multivariante sobre el conjunto de datos de las faunas actuales y fósiles. Finalmente, las faunas de mamíferos del Aragoniense de la Comunidad de Madrid mostraron una predominancia de ambientes semiáridos con varios pulsos de mayor aridez en las biozonas Dc, E y F asociados al evento de enfriamiento global del Mioceno medio

Miocardiopatía por estrés: takotsubo

Clinic. A 83-year-old woman who presented with acute chest pain after an intense emotional stimulus. Diagnostic tests. EKG showed negative T waves in anterior and ultrasensitive troponins were raised. The echocardiogram showed an intensely depressed with akinesia media and distal segments of all sides and apex, with conventional coronary angiography systolic function without significant lesions. The MRI was consistent with stress cardiomyopathy (ECM) takotsubo. Discussion. of uncertain origin, the ECM can mimic myocardial infarction with normal coronary arteries and good prognosis.Clínica. Mujer de 83 años que consultó por dolor torácico agudo tras un estímulo emocional intenso. Pruebas diagnósticas. El electrocardiograma mostró ondas T negativas en cara anterior y las troponinas ultrasensibles estaban elevadas. El ecocardiograma mostró una función sistólica global intensamente deprimida con acinesia de los segmentos medios y distales de todas las caras y el ápex, con coronariografía convencional sin lesiones significativas. La imagen por RM era compatible con miocardiopatía de estrés (MCE-takotsubo). Discusión. De origen incierto, la MCE puede remedar un infarto de miocardio con coronarias normales y buen pronóstico

Actualización de microorganismos frecuentemente declarados al Sistema de Información Microbiológica hasta la semana 26 de 2015 .

[ES] Campylobacter, virus respiratorio sincitial, rotavirus y Chlamydia tracomatis, se encuentran entre los microorganismos más frecuentemente declarados al Centro Nacional de Epidemiología a través del Sistema de Información Microbiológica (SIM). Este trabajo presenta un resumen de los datos recogidos para estos agentes hasta la semana 26 de 2015, y una actualización de los mismos desde 2012, de los laboratorios que han realizado declaración estable durante dicho período. [EN] Campylobacter, respiratory syncytial virus, rotavirus and Chlamydia tracomatis are among the microorganisms more frequently reported to the National Centre of Epidemiology (Spain) through the Microbiological Information System. The current work offers an overview of data collected on these agents through 26th week, 2015, and an update about them, from 2012, from laboratories with stable report

Klebsiella pneumoniae is able to trigger epithelial-mesenchymal transition process in cultured airway epithelial cells

The ability of some bacterial pathogens to activate Epithelial-Mesenchymal Transition normally is a consequence of the persistence of a local chronic inflammatory response or depends on a direct interaction of the pathogens with the host epithelial cells. In this study we monitored the abilities of the K. pneumoniae to activate the expression of genes related to EMT-like processes and the occurrence of phenotypic changes in airway epithelial cells during the early steps of cell infection. We describe changes in the production of intracellular reactive oxygen species and increased HIF-1α mRNA expression in cells exposed to K. pneumoniae infection. We also describe the upregulation of a set of transcription factors implicated in the EMT processes, such as Twist, Snail and ZEB, indicating that the morphological changes of epithelial cells already appreciable after few hours from the K. pneumoniae infection are tightly regulated by the activation of transcriptional pathways, driving epithelial cells to EMT. These effects appear to be effectively counteracted by resveratrol, an antioxidant that is able to exert a sustained scavenging of the intracellular ROS. This is the first report indicating that strains of K. pneumoniae may promote EMT-like programs through direct interaction with epithelial cells without the involvement of inflammatory cells

Long Term Stability of the LHC Superconducting Cryodipoles after Outdoor Storage

The main superconducting dipoles for the LHC are being stored outdoors for periods from a few weeks to several years after conditioning with dry nitrogen gas. Such a storage before installation in the 27 km circumference tunnel may affect not only the mechanical and cryogenic functionality of the cryodipoles but also their quench and field performance. A dedicated task force was established to study all aspects of long term behaviour of the stored cryodipoles, with particular emphasis on electrical and vacuum integrity, quench training behaviour, magnetic field quality, performance of the thermal insulation, mechanical stability of magnet shape and of the interface between cold mass and cryostat, degradation ofmaterials and welds. In particular, one specifically selected cryodipole stored outdoors for more than one year, was retested at cold. In addition, various tests have been carried out on the cryodipole assembly and on the most critical subcomponents to study aspects such as the hygrothermal behaviour of the supporting system and the possible oxidation of the Multi Layer Insulation reflective films. This paper summarizes the main investigations carried out and their results