MutS regulates access of the error-prone DNA polymerase Pol IV to replication sites: a novel mechanism for maintaining replication fidelity

Translesion DNA polymerases (Pol) function in the bypass of template lesions to relieve stalled replication forks but also display potentially deleterious mutagenic phenotypes that contribute to antibiotic resistance in bacteria and lead to human disease. Effective activity of these enzymes requires association with ring-shaped processivity factors, which dictate their access to sites of DNA synthesis. Here, we show for the first time that the mismatch repair protein MutS plays a role in regulating access of the conserved Y-family Pol IV to replication sites. Our biochemical data reveals that MutS inhibits the interaction of Pol IV with the β clamp processivity factor by competing for binding to the ring. Moreover, the MutS–β clamp association is critical for controlling Pol IV mutagenic replication under normal growth conditions. Thus, our findings reveal important insights into a non-canonical function of MutS in the regulation of a replication activity.Fil: Margara, Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Fernández, Marisa Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Argaraña, Carlos Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; ArgentinaFil: Monti, Mariela Roxana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones en Química Biológica de Córdoba. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba; Argentin

Toluene biodegradation in an algal-bacterial airlift photobioreactor: Influence of the biomass concentration and of the presence of an organic phase

Producción CientíficaThe potential of algal-bacterial symbiosis for off-gas abatement was investigated for the first time by comparatively evaluating the performance of a bacterial (CB) and an algal-bacterial (PB) airlift bioreactors during the treatment of a 6 g m−3 toluene laden air emission. The influence of biomass concentration and of the addition of a non-aqueous phase was also investigated. A poor and fluctuating performance was recorded during the initial stages of the experiment, which was attributed to the low biomass concentration present in both reactors and to the accumulation of toxic metabolites. In this sense, an increase in the dilution rate from 0.23 to 0.45 d−1 and in biomass concentration from ∼1 to ∼5 g L−1 resulted in elimination capacities (ECs) of 300 g m−3 h−1 (corresponding to removal efficiencies ∼ 90%). Microalgae activity allowed for a reduction in the emitted CO2 and an increase in dissolved O2 concentration in the PB. However, excess biomass growth over 11 g L−1 hindered light penetration and severely decreased photosynthetic activity. The addition of silicone oil at 20% (on a volume basis) stabilized system performance, leading to dissolved O2 concentrations of 7 mg L−1 and steady ECs of 320 g m−3 h−1 in the PB. The ECs here recorded were considerably higher than those previously reported in toluene-degrading bioreactors. Finally, microbial population analysis by DGGE-sequencing demonstrated the differential specialization of the microbial community in both reactors, likely resulting in different toluene degradation pathways and metabolites production.Ministerio de Economía, Industria y Competitividad (Proyect CTM2015-70442-R)Junta de Castilla y León (programa de apoyo a proyectos de investigación – Ref. VA024U14 y UIC 71

Aplicación Del Análisis Envolvente De Datos En Las Universidades Autónomas De Bolivia. Caso Carreras Ciencias Empresariales

The technical efficiency is a tool that allows to know the situation in which an institution is located. In this context, the objective of the research is to determine the technical efficiency indices of the years 2014 and 2015 in order to carry out a comparative analysis and identify their causes in the careers related to the Economic and Business Sciences of the State Autonomous Public Universities Plurinacional de Bolivia through the application of Data Envelopment Analysis (DEA) as a tool for analysis of technical efficiency. The research constitutes the first work of analysis of university efficiency. 69 studies belonging to 11 Universities of the Bolivian University System are considered as study population. The results show that there are careers with high indices of technical efficiency; on average, there is an increase in technical efficiency between 2014 and 2015; the number of students, graduates and teachers are what determine the rates of technical efficiency in careers

Calidad de servicio y la capacitación del personal del área de logística de la Red de Salud San Juan de Miraflores – Villa María del Triunfo, 2015

El objetivo principal de esta investigación fue establecer la relación correspondiente entre la calidad de servicio y la capacitación del personal del área de logística de la Red de Salud San Juan de Miraflores – Villa María del Triunfo, 2015. Este estudio se enmarca dentro de la investigación descriptiva, el diseño utilizado, corresponde a no experimental transversal y correlacional, la población objetivo de estudio estuvo conformada por 150 trabajadores usuarios de las áreas de logística y la muestra estuvo constituida por los integrantes de la población, por ello se denominó una muestra censal, la técnica utilizada fue la encuesta y el instrumento un cuestionario para cada variable, conformado por 30 preguntas cada uno; dicho instrumento fue sometido a la confiabilidad a través del coeficiente Alfa de Cronbach con los siguientes puntajes: 0,798 para la variable calidad de servicio y 0,790 para la variable capacitación de personal, en los resultados, el 65% de la muestra considera que el nivel de la calidad de servicio es medio y el 60.7% afirma que el nivel de capacitación también es medio. Como conclusión general se indica que existe relación significativa entre la calidad de servicio y capacitación del personal de logística de la red de salud San Juan de Miraflores – Villa María del Triunfo, 2015 con un p = 0.0248 menor que alfa = 0.0

A comparison of machine learning techniques for detection of drug target articles

Important progress in treating diseases has been possible thanks to the identification of drug targets. Drug targets are the molecular structures whose abnormal activity, associated to a disease, can be modified by drugs, improving the health of patients. Pharmaceutical industry needs to give priority to their identification and validation in order to reduce the long and costly drug development times. In the last two decades, our knowledge about drugs, their mechanisms of action and drug targets has rapidly increased. Nevertheless, most of this knowledge is hidden in millions of medical articles and textbooks. Extracting knowledge from this large amount of unstructured information is a laborious job, even for human experts. Drug target articles identification, a crucial first step toward the automatic extraction of information from texts, constitutes the aim of this paper. A comparison of several machine learning techniques has been performed in order to obtain a satisfactory classifier for detecting drug target articles using semantic information from biomedical resources such as the Unified Medical Language System. The best result has been achieved by a Fuzzy Lattice Reasoning classifier, which reaches 98% of ROC area measure.This research paper is supported by Projects TIN2007-67407- C03-01, S-0505/TIC-0267 and MICINN project TEXT-ENTERPRISE 2.0 TIN2009-13391-C04-03 (Plan I + D + i), as well as for the Juan de la Cierva program of the MICINN of SpainPublicad

Influence of Business Incubators Performance on Entrepreneurial Intentions and Its Antecedents during the Pre-incubation Stage

The pre-incubation phase is critical in the forming of entrepreneur?s intention and however it is almost missing in literature. Using the entrepreneurship models based on the beliefs?attitude?intention relationship, a sample of 167 pre-incubated entrepreneurs within the well-known System of Business Incubators of the Monterrey Institute of Technology and Higher Education was surveyed and its statistical analysis developed with Structural Equation Modelling methodology. Our findings confirm that (1) entrepreneur?s perception concerning the incubator?s performance directly and positively affects the desirability, self-efficacy and the facilitating conditions, (2) the perceived desirability and feasibility in entrepreneurship, as well as the first ones, self-efficacy and facilitating conditions, positively influence the entrepreneurial intentions. Furthermore, contrary to the traditional mind-set that supports risk is a barrier, results suggest that (3) the risk perceived by pre-incubated entrepreneurs positively influences entrepreneurial intentions and the desirability that is attributed to that behaviour. Finally, these results also provide insights for organizational leaders and policymakers into managing business incubators and organising their formative programs. The effectiveness of incubators is clear but it is also important to communicate it properly so that incubated individuals can perceive it

IMPACTO ECONÓMICO Y SOCIAL DE LA COVID-19 EN EL PERÚ

COVID-19 is a disease that has not only caused the loss of many human lives, but has also increased the poverty gap, impacting human development. Based on the above, the objective of this research was to identify the economic and social impact of the crisis caused by COVID-19 in Peru. The research was descriptive-explanatory and quantitative, and the design was non-experimental and cross-sectional. The unit of analysis consisted of documents referring to macroeconomic indicators of the States at the global level; in particular, documents detailing macroeconomic indicators at the national level, such as data from the Lima Stock Exchange and projections from the Ministry of Economy and Finance. In terms of data collection techniques and instruments, documentary analysis and the registration form were used, respectively. It was found that, between 2016 and 2019, a sustained growth rate was evidenced at the national level; however, the stoppage of economic activities to prevent the spread of COVID-19, led to a decrease in economic sectors. Based on the above, it was concluded that the sanitary crisis generated by COVID-19 has affected the development of Peru, increasing the levels of poverty and inequality, problems that will only be solved through the development and implementation of public policies that promote the adequate recovery of the national economy.La COVID-19 es una enfermedad que no solo ha generado la pérdida de cuantiosas vidas humanas, sino también ha aumentado la brecha de pobreza, impactando en el desarrollo humano. Con base en lo señalado, esta investigación tuvo como objetivo identificar el impacto económico y social de la crisis causada por la COVID-19 en el Perú. La investigación fue de tipo descriptivo-explicativo y de enfoque cuantitativo, y el diseño fue no experimental y transeccional. La unidad de análisis estuvo conformada por documentos referentes a los indicadores macroeconómicos de los Estados a nivel global, en particular por documentos que detallan los indicadores macroeconómicos a nivel nacional, como los datos de la Bolsa de Valores de Lima y las proyecciones del Ministerio de Economía y Finanzas. En cuanto a la técnicas e instrumentos de recolección de datos, se utilizó el análisis documental y la ficha de registro, respectivamente. Se encontró que, entre 2016 y 2019, se evidenció una tasa de crecimiento sostenida a nivel nacional; sin embargo, la paralización de las actividades económicas para evitar la propagación de la COVID-19, condujeron a un decrecimiento de los sectores económicos. A partir de lo indicado, se concluyó que la crisis sanitaria generada por la COVID-19 ha afectado el desarrollo del Perú, incrementando los niveles de pobreza y desigualdad, problemas que solo serán resueltos a partir de la elaboración y ejecución de políticas públicas, que impulsen la recuperación adecuada de la economía nacional

Influence of perceived risk on entrepreneurial desirability and feasibility: multidimensional approach for nascent entrepreneurs

ABSTRACT: This study examines the influence of perceived risk on entrepreneurial desirability and feasibility as determining factors in the intention to start a self-owned business. Specifically, a multidimensional approach is taken to examine the different types of risks associated with entrepreneurship: economic, social, time, health and personal risks. The results obtained from a sample of 376 new entrepreneurs in Mexico confirm the fact that the perceived desirability and feasibility have a significant and positive effect on the intentions to start their own company. On one hand, the empirical evidence obtained shows a heterogenic effect of the risk dimensions associated with entrepreneurship on the perceived desirability and feasibility in their behaviour. Therefore, it was found that the economic risk associated with entrepreneurship has a negative effect on the feasibility to start a business, but does not significantly influence the desirability of that behaviour. On the other hand, a significant effect from the risk related to health is not seen on desirability and feasibility, but the negative influence of personal risk is empirically supported for both variables. Finally, the results obtained related to social and time risk are contradictory. Social risk negatively influences entrepreneurship desirability, but a positive effect is observed on the feasibility to create a business. For its part, the empirical evidence obtained does not support any effect of the time risk on the perceived feasibility of starting a business, but there is a positive on entrepreneurship desirability. These results, which are contrary to the traditional concept of risk as a barrier to entrepreneurship, are justified by the vocational nature of this behaviour and by the social sacrifices that are sometimes necessary to be able to make a business work

La dimensión literaria de las Cartas Anuas de América, siglo XVII

Intentamos -en este estudio- analizar las Cartas como textos de una complejidad expresiva que   expone una trayectoria figurativa novedosa -desde las preceptivas subyacentes- en la configuración de mundos de América. Esto supone articular un instrumento metodológico desde un paradigma teórico entre la fenomenología y la hermenéutica con algunas revisiones e integraciones de enfoques semiológicos y retóricos. Desde el eje fenomenológico-hermenéutico diferenciamos referencia / significado/ sentido. Aislamos procesos figurales del texto en la configuración semántica intensional/extensional