138 research outputs found
Recurring cluster and operon assembly for Phenylacetate degradation genes
<p>Abstract</p> <p>Background</p> <p>A large number of theories have been advanced to explain why genes involved in the same biochemical processes are often co-located in genomes. Most of these theories have been dismissed because empirical data do not match the expectations of the models. In this work we test the hypothesis that cluster formation is most likely due to a selective pressure to gradually co-localise protein products and that operon formation is not an inevitable conclusion of the process.</p> <p>Results</p> <p>We have selected an exemplar well-characterised biochemical pathway, the phenylacetate degradation pathway, and we show that its complex history is only compatible with a model where a selective advantage accrues from moving genes closer together. This selective pressure is likely to be reasonably weak and only twice in our dataset of 102 genomes do we see independent formation of a complete cluster containing all the catabolic genes in the pathway. Additionally, <it>de novo </it>clustering of genes clearly occurs repeatedly, even though recombination should result in the random dispersal of such genes in their respective genomes. Interspecies gene transfer has frequently replaced <it>in situ </it>copies of genes resulting in clusters that have similar content but very different evolutionary histories.</p> <p>Conclusion</p> <p>Our model for cluster formation in prokaryotes, therefore, consists of a two-stage selection process. The first stage is selection to move genes closer together, either because of macromolecular crowding, chromatin relaxation or transcriptional regulation pressure. This proximity opportunity sets up a separate selection for co-transcription.</p
The K2 Mission: Characterization and Early results
The K2 mission will make use of the Kepler spacecraft and its assets to
expand upon Kepler's groundbreaking discoveries in the fields of exoplanets and
astrophysics through new and exciting observations. K2 will use an innovative
way of operating the spacecraft to observe target fields along the ecliptic for
the next 2-3 years. Early science commissioning observations have shown an
estimated photometric precision near 400 ppm in a single 30 minute observation,
and a 6-hour photometric precision of 80 ppm (both at V=12). The K2 mission
offers long-term, simultaneous optical observation of thousands of objects at a
precision far better than is achievable from ground-based telescopes. Ecliptic
fields will be observed for approximately 75-days enabling a unique exoplanet
survey which fills the gaps in duration and sensitivity between the Kepler and
TESS missions, and offers pre-launch exoplanet target identification for JWST
transit spectroscopy. Astrophysics observations with K2 will include studies of
young open clusters, bright stars, galaxies, supernovae, and asteroseismology.Comment: 25 pages, 11 figures, Accepted to PAS
Precision asteroseismology of the pulsating white dwarf GD 1212 using a two-wheel-controlled Kepler spacecraft
We present a preliminary analysis of the cool pulsating white dwarf GD 1212,
enabled by more than 11.5 days of space-based photometry obtained during an
engineering test of the two-reaction-wheel-controlled Kepler spacecraft. We
detect at least 19 independent pulsation modes, ranging from 828.2-1220.8 s,
and at least 17 nonlinear combination frequencies of those independent
pulsations. Our longest uninterrupted light curve, 9.0 days in length,
evidences coherent difference frequencies at periods inaccessible from the
ground, up to 14.5 hr, the longest-period signals ever detected in a pulsating
white dwarf. These results mark some of the first science to come from a
two-wheel-controlled Kepler spacecraft, proving the capability for
unprecedented discoveries afforded by extending Kepler observations to the
ecliptic.Comment: 8 pages, 4 figures, accepted for publication in The Astrophysical
Journa
The effect of bone microstructure on the initiation and growth of microcracks.
Osteonal bone is often compared to a composite material and to metals as discontinuities within the material may provide sites of stress concentration for crack initiation and serve as barriers to crack growth. However, little experimental data exist to back up these hypotheses. Fluorescent chelating agents were applied at specific intervals to bone specimens fatigue tested in cyclic compression at a stress range of 80 MPa. The failed specimens were sectioned and labelled microcracks identified using UV epifluorescence microscopy. Microcrack lengths were measured and their relationship to cement lines surrounding secondary osteons recorded. Microcrack length at the time of encountering a cement line was also measured. Microcracks of less than 100mum stopped growing when they encountered a cement line. Microcracks of greater than 100mum in length continued to grow after encountering a cement line surrounding an osteon. Only microcracks greater than 300mum in length were capable of penetrating osteons and these microcracks were the only ones which were observed to cause failure in the specimen. These experimental data support the hypothesis that secondary osteons act as barriers to crack propagation in compact bone. However, it shows that this microstructural barrier effect is dependent on the crack length at the time of encountering an osteon. For the vast majority of cracks, osteons act as barriers to growth but for the minority of cracks that are long enough and do break through the cement line, an osteon may actually act as a weakness in the bone and facilitate crack propagation
Validation of Kepler's Multiple Planet Candidates. III: Light Curve Analysis & Announcement of Hundreds of New Multi-planet Systems
The Kepler mission has discovered over 2500 exoplanet candidates in the first
two years of spacecraft data, with approximately 40% of them in candidate
multi-planet systems. The high rate of multiplicity combined with the low rate
of identified false-positives indicates that the multiplanet systems contain
very few false-positive signals due to other systems not gravitationally bound
to the target star (Lissauer, J. J., et al., 2012, ApJ 750, 131). False
positives in the multi- planet systems are identified and removed, leaving
behind a residual population of candidate multi-planet transiting systems
expected to have a false-positive rate less than 1%. We present a sample of 340
planetary systems that contain 851 planets that are validated to substantially
better than the 99% confidence level; the vast majority of these have not been
previously verified as planets. We expect ~2 unidentified false-positives
making our sample of planet very reliable. We present fundamental planetary
properties of our sample based on a comprehensive analysis of Kepler light
curves and ground-based spectroscopy and high-resolution imaging. Since we do
not require spectroscopy or high-resolution imaging for validation, some of our
derived parameters for a planetary system may be systematically incorrect due
to dilution from light due to additional stars in the photometric aperture.
None the less, our result nearly doubles the number of verified exoplanets.Comment: 138 pages, 8 Figures, 5 Tables. Accepted for publications in the
Astrophysical Journa
Solving the mode identification problem in asteroseismology of F stars observed with Kepler
Asteroseismology of F-type stars has been hindered by an ambiguity in
identification of their oscillation modes. The regular mode pattern that makes
this task trivial in cooler stars is masked by increased linewidths. The
absolute mode frequencies, encapsulated in the asteroseismic variable epsilon,
can help solve this impasse because the values of epsilon implied by the two
possible mode identifications are distinct. We find that the correct epsilon
can be deduced from the effective temperature and the linewidths and we apply
these methods to a sample of solar-like oscillators observed with Kepler.Comment: 7 pages, 4 figures, 1 table, accepted for publication in The
Astrophysical Journal Letter
Planetary Candidates Observed by Kepler. VIII. A Fully Automated Catalog With Measured Completeness and Reliability Based on Data Release 25
We present the Kepler Object of Interest (KOI) catalog of transiting
exoplanets based on searching four years of Kepler time series photometry (Data
Release 25, Q1-Q17). The catalog contains 8054 KOIs of which 4034 are planet
candidates with periods between 0.25 and 632 days. Of these candidates, 219 are
new and include two in multi-planet systems (KOI-82.06 and KOI-2926.05), and
ten high-reliability, terrestrial-size, habitable zone candidates. This catalog
was created using a tool called the Robovetter which automatically vets the
DR25 Threshold Crossing Events (TCEs, Twicken et al. 2016). The Robovetter also
vetted simulated data sets and measured how well it was able to separate TCEs
caused by noise from those caused by low signal-to-noise transits. We discusses
the Robovetter and the metrics it uses to sort TCEs. For orbital periods less
than 100 days the Robovetter completeness (the fraction of simulated transits
that are determined to be planet candidates) across all observed stars is
greater than 85%. For the same period range, the catalog reliability (the
fraction of candidates that are not due to instrumental or stellar noise) is
greater than 98%. However, for low signal-to-noise candidates between 200 and
500 days around FGK dwarf stars, the Robovetter is 76.7% complete and the
catalog is 50.5% reliable. The KOI catalog, the transit fits and all of the
simulated data used to characterize this catalog are available at the NASA
Exoplanet Archive.Comment: 61 pages, 23 Figures, 9 Tables, Accepted to The Astrophysical Journal
Supplement Serie
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