300 research outputs found
The Planetary Nebulae Spectrograph: the green light for Galaxy Kinematics
Planetary nebulae are now well established as probes of galaxy dynamics and
as standard candles in distance determinations. Motivated by the need to
improve the efficiency of planetary nebulae searches and the speed with which
their radial velocities are determined, a dedicated instrument - the Planetary
Nebulae Spectrograph or PN.S - has been designed and commissioned at the 4.2m
William Herschel Telescope. The high optical efficiency of the spectrograph
results in the detection of typically ~ 150 PN in galaxies at the distance of
the Virgo cluster in one night of observations. In the same observation the
radial velocities are obtained with an accuracy of ~ 20 km/sComment: Accepted by PASP, to appear November 2002; the figures have been
degraded for archival purpose
Ultrastructural changes of the intracellular surfactant pool in a rat model of lung transplantation-related events
<p>Abstract</p> <p>Background</p> <p>Ischemia/reperfusion (I/R) injury, involved in primary graft dysfunction following lung transplantation, leads to inactivation of intra-alveolar surfactant which facilitates injury of the blood-air barrier. The alveolar epithelial type II cells (AE2 cells) synthesize, store and secrete surfactant; thus, an intracellular surfactant pool stored in lamellar bodies (Lb) can be distinguished from the intra-alveolar surfactant pool. The aim of this study was to investigate ultrastructural alterations of the intracellular surfactant pool in a model, mimicking transplantation-related procedures including flush perfusion, cold ischemia and reperfusion combined with mechanical ventilation.</p> <p>Methods</p> <p>Using design-based stereology at the light and electron microscopic level, number, surface area and mean volume of AE2 cells as well as number, size and total volume of Lb were determined in a group subjected to transplantation-related procedures including both I/R injury and mechanical ventilation (I/R group) and a control group.</p> <p>Results</p> <p>After I/R injury, the mean number of Lb per AE2 cell was significantly reduced compared to the control group, accompanied by a significant increase in the luminal surface area per AE2 cell in the I/R group. This increase in the luminal surface area correlated with the decrease in surface area of Lb per AE2. The number-weighted mean volume of Lb in the I/R group showed a tendency to increase.</p> <p>Conclusion</p> <p>We suggest that in this animal model the reduction of the number of Lb per AE2 cell is most likely due to stimulated exocytosis of Lb into the alveolar space. The loss of Lb is partly compensated by an increased size of Lb thus maintaining total volume of Lb per AE2 cell and lung. This mechanism counteracts at least in part the inactivation of the intra-alveolar surfactant.</p
Synthetic High-Resolution Line Spectra of Star-Forming Galaxies Below 1200A
We have generated a set of far-ultraviolet stellar libraries using spectra of
OB and Wolf-Rayet stars in the Galaxy and the Large and Small Magellanic Cloud.
The spectra were collected with the Far Ultraviolet Spectroscopic Explorer and
cover a wavelength range from 1003.1 to 1182.7A at a resolution of 0.127A. The
libraries extend from the earliest O- to late-O and early-B stars for the
Magellanic Cloud and Galactic libraries, respectively. Attention is paid to the
complex blending of stellar and interstellar lines, which can be significant,
especially in models using Galactic stars. The most severe contamination is due
to molecular hydrogen. Using a simple model for the H line strength, we
were able to remove the molecular hydrogen lines in a subset of Magellanic
Cloud stars. Variations of the photospheric and wind features of CIII 1176, OVI
1032, 1038, PV 1118, 1128, and SIV 1063, 1073, 1074 are discussed as a function
of temperature and luminosity class. The spectral libraries were implemented
into the LavalSB and Starburst99 packages and used to compute a standard set of
synthetic spectra of star-forming galaxies. Representative spectra are
presented for various initial mass functions and star formation histories. The
valid parameter space is confined to the youngest ages of less than 10 Myr for
an instantaneous burst, prior to the age when incompleteness of spectral types
in the libraries sets in. For a continuous burst at solar metallicity, the
parameter space is not limited. The suite of models is useful for interpreting
the restframe far-ultraviolet in local and high-redshift galaxies.Comment: 33 pages including 13 figures, accepted for publication in Ap
GYES, a multifibre spectrograph for the CFHT
We have chosen the name of GYES, one of the mythological giants with one
hundred arms, offspring of Gaia and Uranus, for our instrument study of a
multifibre spectrograph for the prime focus of the Canada-France-Hawaii
Telescope. Such an instrument could provide an excellent ground-based
complement for the Gaia mission and a northern complement to the HERMES project
on the AAT. The CFHT is well known for providing a stable prime focus
environment, with a large field of view, which has hosted several imaging
instruments, but has never hosted a multifibre spectrograph. Building upon the
experience gained at GEPI with FLAMES-Giraffe and X-Shooter, we are
investigating the feasibility of a high multiplex spectrograph (about 500
fibres) over a field of view 1 degree in diameter. We are investigating an
instrument with resolution in the range 15000 to 30000, which should provide
accurate chemical abundances for stars down to 16th magnitude and radial
velocities, accurate to 1 km/s for fainter stars. The study is led by
GEPI-Observatoire de Paris with a contribution from Oxford for the study of the
positioner. The financing for the study comes from INSU CSAA and Observatoire
de Paris. The conceptual study will be delivered to CFHT for review by October
1st 2010.Comment: Contributed talk at the Gaia ELSA conference 2010, S\`evres 7-11 June
2010, to be published on the EAS Series, Editors: C. Turon, F. Arenou & F.
Meynadie
Traffic Instabilities in Self-Organized Pedestrian Crowds
In human crowds as well as in many animal societies, local interactions among
individuals often give rise to self-organized collective organizations that
offer functional benefits to the group. For instance, flows of pedestrians
moving in opposite directions spontaneously segregate into lanes of uniform
walking directions. This phenomenon is often referred to as a smart collective
pattern, as it increases the traffic efficiency with no need of external
control. However, the functional benefits of this emergent organization have
never been experimentally measured, and the underlying behavioral mechanisms
are poorly understood. In this work, we have studied this phenomenon under
controlled laboratory conditions. We found that the traffic segregation
exhibits structural instabilities characterized by the alternation of organized
and disorganized states, where the lifetime of well-organized clusters of
pedestrians follow a stretched exponential relaxation process. Further analysis
show that the inter-pedestrian variability of comfortable walking speeds is a
key variable at the origin of the observed traffic perturbations. We show that
the collective benefit of the emerging pattern is maximized when all
pedestrians walk at the average speed of the group. In practice, however, local
interactions between slow- and fast-walking pedestrians trigger global
breakdowns of organization, which reduce the collective and the individual
payoff provided by the traffic segregation. This work is a step ahead toward
the understanding of traffic self-organization in crowds, which turns out to be
modulated by complex behavioral mechanisms that do not always maximize the
group's benefits. The quantitative understanding of crowd behaviors opens the
way for designing bottom-up management strategies bound to promote the
emergence of efficient collective behaviors in crowds.Comment: Article published in PLoS Computational biology. Freely available
here:
http://www.ploscompbiol.org/article/info%3Adoi%2F10.1371%2Fjournal.pcbi.100244
The practice of glycaemic control in intensive care units: A multicentre survey of nursing and medical professionals.
AIMS AND OBJECTIVES: To determine the views of nurses and physicians working in intensive care units (ICU) about the aims of glycaemic control and use of their protocols. BACKGROUND: Evidence about the optimal aims and methods for glycaemic control in ICU is controversial, and current local protocols guiding practice differ between ICUs, both nationally and internationally. The views of professionals on glycaemic control can influence their practice. DESIGN: Cross-sectional, multicentre, survey-based study. METHODS: An online short survey was sent to all physicians and nurses of seven ICUs, including questions on effective glycaemic control, treatment of hypoglycaemia and deviations from protocols' instructions. STROBE reporting guidelines were followed. RESULTS: Over half of the 40 respondents opined that a patient spending <75% admission time within the target glycaemic levels constituted poor glycaemic control. Professionals with more than 5Â years of experience were more likely to rate a patient spending 50%-74% admission time within target glycaemic levels as poor than less experienced colleagues. Physicians were more likely to rate a patient spending <50% admission time within target as poor than nurses. There was general agreement on how professionals would rate most deviations from their protocols. Nurses were more likely to rate insulin infusions restarted late and incorrect dosage of rescue glucose as major deviations than physicians. Most professionals agreed on when they would treat hypoglycaemia. CONCLUSIONS: When surveyed on various aspects of glycaemic control, ICU nurses and physicians often agreed, although there were certain areas of disagreement, in which their profession and level of experience seemed to play a role. RELEVANCE TO CLINICAL PRACTICE: Differing views on glycaemic control amongst professionals may affect their practice and, thus, could lead to health inequalities. Clinical leads and the multidisciplinary ICU team should assess and, if necessary, address these differing opinions.Nottingham University Hospitals (NUH) Charity and the NUH Department of Research and Innovation
University of Nottingham School of Health Sciences director of research small grant
Vascular Endothelial Growth Factor (VEGF) isoform expression and activity in human and murine lung injury
<p>Abstract</p> <p>Background</p> <p>The properties of vascular endothelial growth factor (VEGF) as a potent vascular permogen and mitogen have led to investigation of its potential role in lung injury. Alternate spliced VEGF transcript generates several isoforms with potentially differing functions. The purpose of this study was to determine VEGF isoform expression and source in normal and ARDS subjects and investigate the expression and regulation of VEGF isoforms by human alveolar type 2 (ATII) cells.</p> <p>Methods</p> <p>VEGF protein expression was assessed immunohistochemically in archival normal and ARDS human lung tissue. VEGF isoform mRNA expression was assessed in human and murine lung tissue. Purified ATII cells were cultured with proinflammatory cytokines prior to RNA extraction/cell supernatant sampling/proliferation assay.</p> <p>Measurements and Main Results</p> <p>VEGF was expressed on alveolar epithelium, vascular endothelium and alveolar macrophages in normal and ARDS human lung tissue. Increases in VEGF expression were detected in later ARDS in comparison to both normal subjects and early ARDS (p < 0.001). VEGF<sub>121</sub>, VEGF<sub>165 </sub>and VEGF<sub>189 </sub>isoform mRNA expression increased in later ARDS (p < 0.05). The ratio of soluble to cell-associated isoforms was lower in early ARDS than normal subjects and later ARDS and also in murine lung injury. ATII cells constitutionally produced VEGF<sub>165 </sub>and VEGF<sub>121 </sub>protein which was increased by LPS (p < 0.05). VEGF<sub>165 </sub>upregulated ATII cell proliferation (p < 0.001) that was inhibited by soluble VEGF receptor 1 (<it>sflt</it>) (p < 0.05).</p> <p>Conclusion</p> <p>These data demonstrate that changes in VEGF isoform expression occur in ARDS which may be related to their production by and mitogenic effect on ATII cells; with potentially significant clinical consequences.</p
Ionization Structure and Spectra of Iron in Gaseous Nebulae}
The emission spectra and the ionization structure of the low ionization
stages of iron, Fe I--IV, in gaseous nebulae are studied. This work includes:
(i) new atomic data: photoionization cross sections, total e-ion recombination
rates, excitation collision strengths, and transition probabilities; (ii)
detailed study of excitation mechanisms for the [Fe II], [Fe III], and [Fe IV]
emission, and spectroscopic analysis of the observed IR, optical, and UV
spectra; (iii) study of the physical structure and kinematics of the nebulae
and their ionization fronts. Spectral analysis of the well observed Orion
nebula is carried out as a test case, using extensive collisional-radiative and
photoionization models. It is shown that the [Fe II] emission from the Orion
nebula is predominantly excited via electron collisions in high density
partially ionized zones; radiative fluorescence is relatively less effective.
Further evidence for high density zones is derived from the [O I] and [Ni II]
spectral lines, as well as from the kinematic measurements of ionic species in
the nebula. The ionization structure of iron in Orion is modeled using the
newly calculated atomic data, showing some significant differences from
previous models. The new model suggests a fully ionized H II region at
densities on the order of cm, and a dynamic partially ionized H
II/H I region at densities of \cm3. Photoionization models also
indicate that the optical [O I] and [Fe II] emission originates in high density
partially ionized regions within ionization fronts. The gas phase iron
abundance in Orion is estimated from observed spectra.Comment: AAS LaTex, 60 pages 18 figures. Astrophysical Journal. in pres
Soluble receptor for advanced glycation end products in COPD: relationship with emphysema and chronic cor pulmonale: a case-control study
<p>Abstract</p> <p>Background</p> <p>The receptor for advanced glycation end products (RAGE) is a multiligand signal transduction receptor that can initiate and perpetuate inflammation. Its soluble isoform (sRAGE) acts as a decoy receptor for RAGE ligands, and is thought to afford protection against inflammation. With the present study, we aimed at determining whether circulating sRAGE is correlated with emphysema and chronic cor pulmonale in chronic obstructive pulmonary disease (COPD).</p> <p>Methods</p> <p>In 200 COPD patients and 201 age- and sex-matched controls, we measured lung function by spirometry, and sRAGE by ELISA method. We also measured the plasma levels of two RAGE ligands, N-epsilon-carboxymethyl lysine and S100A12, by ELISA method. In the COPD patients, we assessed the prevalence and severity of emphysema by computed tomography (CT), and the prevalence of chronic cor pulmonale by echocardiography. Multiple quantile regression was used to assess the effects of emphysema, chronic cor pulmonale, smoking history, and comorbid conditions on the three quartiles of sRAGE.</p> <p>Results</p> <p>sRAGE was significantly lower (p = 0.007) in COPD patients (median 652 pg/mL, interquartile range 484 to 1076 pg/mL) than in controls (median 869 pg/mL, interquartile range 601 to 1240 pg/mL), and was correlated with the severity of emphysema (p < 0.001), the lower the level of sRAGE the greater the degree of emphysema on CT. The relationship remained statistically significant after adjusting for smoking history and comorbid conditions. In addition, sRAGE was significantly lower in COPD patients with chronic cor pulmonale than in those without (p = 0.002). Such difference remained statistically significant after adjusting for smoking history, comorbidities, and emphysema severity. There was no significant difference in the plasma levels of the two RAGE ligands between cases and controls.</p> <p>Conclusions</p> <p>sRAGE is significantly lower in patients with COPD than in age- and sex-matched individuals without airflow obstruction. Emphysema and chronic cor pulmonale are independent predictors of reduced sRAGE in COPD.</p
CCAAT/Enhancer-Binding Protein γ Is a Critical Regulator of IL-1β-Induced IL-6 Production in Alveolar Epithelial Cells
CCAAT/enhancer binding protein γ (C/EBPγ) is a member of the C/EBP family of transcription factors, which lacks known activation domains. C/EBPγ was originally described as an inhibitor of C/EBP transactivation potential. However, previous study demonstrates that C/EBPγ augments the C/EBPβ stimulatory activity in lipopolysaccharide induction of IL-6 promoter in a B lymphoblast cell line. These data indicate a complexing functional role for C/EBPγ in regulating gene expression. Furthermore, the expression and function of C/EBPγ during inflammation are still largely unknown. In this study, we demonstrate that C/EBPγ activation was induced by IL-1β treatment in lung epithelial cells. Importantly, we demonstrate for the first time that C/EBPγ plays a critical role in regulating IL-1β-induced IL-6 expression in both mouse primary alveolar type II epithelial cells and a lung epithelial cell line, MLE12. We further provide the evidence that C/EBPγ inhibits IL-6 expression by inhibiting C/EBPβ but not NF-κB stimulatory activity in MLE12 cells. These findings suggest that C/EBPγ is a key transcription factor that regulates the IL-6 expression in alveolar epithelial cells, and may play an important regulatory role in lung inflammatory responses
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