ALICE potential for heavy-flavour physics

The Large Hadron Collider (LHC), where lead nuclei will collide at the unprecedented c.m.s. energy of 5.5 TeV per nucleon-nucleon pair, will offer new and unique opportunities for the study of the properties of strongly interacting matter at high energy density over extended volumes. We will briefly explain why heavy-flavour particles are well-suited tools for such a study and we will describe how the ALICE experiment is preparing to make use of these tools.Comment: 6 pages, 3 figures, prepared for the Proceedings of "Strange Quark Matter 2007", Levoca, Slovaki

Birds as bio-indicators and as tools to evaluate restoration measures

Within the RIPIDURABLE Project*, birds were used to characterize ecosystems*, to monitor environmental changes and to assess results of restoration measures. Bird surveys were carried out at different space and time scales using standardised point count methods on 8 watercourses in Portugal and France. Several aspects of riparian breeding bird community variation were assessed: along a decreasing gradient of vegetation complexity, along and upstream-downstream gradient, with different surrounding landscapes, with time, with management status, with time and management status, before and after river rehabilitation. Birds appear to be new and reliable indicators for assessing restoration of riparian ecosystems, complementary to those traditionally used

Reducing conditions favor magnetosome production in magnetospirillum magneticum AMB-1

Magnetotactic bacteria (MTB) are a heterogeneous group of Gram-negative prokaryotes, which all produce special magnetic organelles called magnetosomes. The magnetosome consists of a magnetic nanoparticle, either magnetite (Fe3O4) or greigite (Fe3S4), embedded in a membrane, which renders the systems colloidaly stable, a desirable property for biotechnological applications. Although these bacteria are able to regulate the formation of magnetosomes through a biologically-controlled mechanism, the environment in general and the physico-chemical conditions surrounding the cells in particular also influence biomineralization. This work thus aims at understanding how such external conditions, in particular the extracellular oxidation reduction potential, influence magnetite formation in the strain Magnetospirillum magneticum AMB-1. Controlled cultivation of the microorganisms was performed at different redox potential in a bioreactor and the formation of magnetosomes was assessed by microscopic and spectroscopic techniques. Our results show that the formation of magnetosomes is inhibited at the highest potential tested (0 mV), whereas biomineralization is facilitated under reduced conditions (-500 mV). This result improves the understanding of the biomineralization process in MTB and provides useful information in sight of a large scale production of magnetosomes for different applications. © 2019 Olszewska-Widdrat, Schiro, Reichel and Faivre

BIRDS AND SUSTAINABLE MANAGEMENT IN MEDITERRANEAN RIPARIAN AREAS: Bird studies in the RIPIDURABLE project

RIPIDURABLE is an INTERREG IIIC European Program involving 10 partners from Portugal, Spain, France and Greece. Scientists, technicians and local/regional authorities worked together to integrate knowledge, knowhow and practice, towards a rational conservation management of riparian zones. Birds can be used to characterize ecosystems, to monitor environmental changes or to assess results of restoration measures. We have conducted field surveys in order to assess breeding bird communities associated to riparian galleries in several watercourses, including issues as the relation with natural vegetation profiles, the influence of the surrounding matrix, the evolution with time or with habitat degradation, the effects of rehabilitation measures, and/or the importance of riparian galleries as ecological corridors for birds. Surveys at different space and time scales were carried out using standardised point count methods, on 8 watercourses in Portugal and France. We focused on different aspects of riparian breeding bird community variation: along a decreasing gradient of vegetal complexity (Tagus Basin), along an upstream-downstream gradient (Allier), with different surrounding landscapes (Sado, Guadiana and Tagus Basin), with time (Alcáçovas at a 10 years interval, Allier at 16 years interval), with management status (Vidourle), with time & management status (Rhône delta at a 12 years intervals), before & after river rehabilitation (Gandum). In addition we studied the dispersal of Barn Owls from upper Tagus Estuary along riparian corridors (TytoTagus Project), and also the importance of riparian habitats of the Guadiana basin on the autumn migration of trans-Saharan birds across the Iberian Peninsula. The RIPIDURABLE project offered the opportunity for further research currently included in national programs such as the Plan Loire Grandeur Nature which allows long term studies on riparian birds. Herein we briefly present some of the preliminary results of the bird studies carried out by partners from Portugal and France

Cytotoxics compounded sterile preparation control by HPLC during a 16-month assessment in a French university hospital: importance of the mixing bags step

The Centralized Chemotherapy Reconstitution Unit (CCRU) of Paul Brousse Hospital Pharmacy Department assessed the reliability of its Cytotoxics Compounded Sterile Products (CCSP) preparation method in order to improve its CCSP quality assurance system. Five cytotoxic drugs — gemcitabine, 5-fluorouracil, docetaxel, paclitaxel, and oxaliplatin — were assayed by high performance liquid chromatography (HPLC) to determine CCSP concentration. During the observation period, 23,892 CCSP were prepared. Overall, 12,964 preparations contained one of the five analyzed drugs; 7382 (56.9%) out of 12,964 CCSP were analyzed by HPLC; 646 (8.8%) out of 7382 concentrations were outside ± 20% of the prescribed dose; 544 (84.2%) out of 646 were post-administration results and could not be verified. Out of 102 (15.8%) pre-administration results that were re-tested after re-shaking, 94 (92.2%) were found to be acceptable upon re-testing, and 8 (7.8%) were confirmed to be unacceptable and needed to be re-compounded. The 8.8% of tested CCSP were outside ± 20% of the prescribed dose, but extrapolating the results on re-tested CCSP, we can say that our CCSP preparation is reliable with an estimation of only 0.7% of 7382 CCSP analyzed, confirmed as being ± 20% outside the prescribed dose. Nevertheless, this ± 20% magnitude of error should be reduced. Based on pre-administration results, the primary cause of concentration errors appeared to be insufficient mixing of the finished product. Most CCSP dosages occurred after it had been administered, the organization should, therefore, be improved to include testing all CCSP prior to administration. Pharmaceutical companies should endeavor to manufacture compounded injectible drugs in a ‘ready to use’ form and provide vehicles in accurate volumes in order to improve compounding precision

Targeting cancer cell metabolism in pancreatic adenocarcinoma.

Pancreatic ductal adenocarcinoma (PDAC) is expected to become the second leading cause of cancer death by 2030. Current therapeutic options are limited, warranting an urgent need to explore innovative treatment strategies. Due to specific microenvironment constraints including an extensive desmoplastic stroma reaction, PDAC faces major metabolic challenges, principally hypoxia and nutrient deprivation. Their connection with oncogenic alterations such as KRAS mutations has brought metabolic reprogramming to the forefront of PDAC therapeutic research. The Warburg effect, glutamine addiction, and autophagy stand as the most important adaptive metabolic mechanisms of cancer cells themselves, however metabolic reprogramming is also an important feature of the tumor microenvironment, having a major impact on epigenetic reprogramming and tumor cell interactions with its complex stroma. We present a comprehensive overview of the main metabolic adaptations contributing to PDAC development and progression. A review of current and future therapies targeting this range of metabolic pathways is provided

Reducing Conditions Favor Magnetosome Production in Magnetospirillum magneticum AMB-1

Magnetotactic bacteria (MTB) are a heterogeneous group of Gram-negative prokaryotes, which all produce special magnetic organelles called magnetosomes. The magnetosome consists of a magnetic nanoparticle, either magnetite (Fe3O4) or greigite (Fe3S4), embedded in a membrane, which renders the systems colloidaly stable, a desirable property for biotechnological applications. Although these bacteria are able to regulate the formation of magnetosomes through a biologically-controlled mechanism, the environment in general and the physico–chemical conditions surrounding the cells in particular also influence biomineralization. This work thus aims at understanding how such external conditions, in particular the extracellular oxidation reduction potential, influence magnetite formation in the strain Magnetospirillum magneticum AMB-1. Controlled cultivation of the microorganisms was performed at different redox potential in a bioreactor and the formation of magnetosomes was assessed by microscopic and spectroscopic techniques. Our results show that the formation of magnetosomes is inhibited at the highest potential tested (0 mV), whereas biomineralization is facilitated under reduced conditions (-500 mV). This result improves the understanding of the biomineralization process in MTB and provides useful information in sight of a large scale production of magnetosomes for different applications

Eutectic Colony Formation: A Stability Analysis

Experiments have widely shown that a steady-state lamellar eutectic solidification front is destabilized on a scale much larger than the lamellar spacing by the rejection of a dilute ternary impurity and forms two-phase cells commonly referred to as `eutectic colonies'. We extend the stability analysis of Datye and Langer for a binary eutectic to include the effect of a ternary impurity. We find that the expressions for the critical onset velocity and morphological instability wavelength are analogous to those for the classic Mullins-Sekerka instability of a monophase planar interface, albeit with an effective surface tension that depends on the geometry of the lamellar interface and, non-trivially, on interlamellar diffusion. A qualitatively new aspect of this instability is the occurence of oscillatory modes due to the interplay between the destabilizing effect of the ternary impurity and the dynamical feedback of the local change in lamellar spacing on the front motion. In a transient regime, these modes lead to the formation of large scale oscillatory microstructures for which there is recent experimental evidence in a transparent organic system. Moreover, it is shown that the eutectic front dynamics on a scale larger than the lamellar spacing can be formulated as an effective monophase interface free boundary problem with a modified Gibbs-Thomson condition that is coupled to a slow evolution equation for the lamellar spacing. This formulation provides additional physical insights into the nature of the instability and a simple means to calculate an approximate stability spectrum. Finally, we investigate the influence of the ternary impurity on a short wavelength oscillatory instability that is already present at off-eutectic compositions in binary eutectics.Comment: 26 pages RevTex, 14 figures (28 EPS files); some minor changes; references adde