Investigating the origin of cyclical wind variability in hot, massive stars - I. On the dipolar magnetic field hypothesis

OB stars exhibit various types of spectral variability associated with wind structures, including the apparently ubiquitous discrete absorption components (DACs). These are proposed to be caused by either magnetic fields or non-radial pulsations (NRPs). In this paper, we evaluate the possible relation between large-scale, dipolar magnetic fields and the DAC phenomenon by investigating the magnetic properties of a sample of 13 OB stars exhibiting well-documented DAC behaviour. Using high-precision spectropolarimetric data acquired in part in the context of the Magnetism in Massive Stars (MiMeS) project, we find no evidence for surface dipolar magnetic fields in any of these stars. Using Bayesian inference, we compute upper limits on the strengths of the fields and use these limits to assess two potential mechanisms by which the field may influence wind outflow: magnetic wind confinement and local photospheric brightness enhancements. Within the limits we derive, both mechanisms fail to provide a systematic process capable of producing DACs in all of the stars of our sample. Therefore, this implies that dipolar fields are highly unlikely to be responsible for these structures in all massive stars, meaning that some other mechanism must come into play.Comment: 17 pages, 6 figures, accepted for publication in MNRA

The MiMeS Project: Overview and Current Status

The Magnetism in Massive Stars (MiMeS) Project is a consensus collaboration among many of the foremost international researchers of the physics of hot, massive stars, with the basic aim of understanding the origin, evolution and impact of magnetic fields in these objects. At the time of writing, MiMeS Large Programs have acquired over 950 high-resolution polarised spectra of about 150 individual stars with spectral types from B5-O4, discovering new magnetic fields in a dozen hot, massive stars. The quality of this spectral and magnetic mat\'eriel is very high, and the Collaboration is keen to connect with colleagues capable of exploiting the data in new or unforeseen ways. In this paper we review the structure of the MiMeS observing programs and report the status of observations, data modeling and development of related theory.Comment: Proceedings of IAUS272: Active OB star

Discovery Of A Magnetic Field In The Rapidly Rotating O-Type Secondary Of The Colliding-Wind Binary HD 47129 (Plaskett\u27s Star)

We report the detection of a strong, organized magnetic field in the secondary component of the massive O8III/I+O7.5V/III double-lined spectroscopic binary system HD 47129 (Plaskett\u27s star) in the context of the Magnetism in Massive Stars survey. Eight independent Stokes V observations were acquired using the Echelle SpectroPolarimetric Device for the Observations of Stars (ESPaDOnS) spectropolarimeter at the Canada-France-Hawaii Telescope and the Narval spectropolarimeter at the Telescope Bernard Lyot. Using least-squares deconvolution we obtain definite detections of signal in Stokes V in three observations. No significant signal is detected in the diagnostic null (N) spectra. The Zeeman signatures are broad and track the radial velocity of the secondary component; we therefore conclude that the rapidly rotating secondary component is the magnetized star. Correcting the polarized spectra for the line and continuum of the (sharp-lined) primary, we measured the longitudinal magnetic field from each observation. The longitudinal field of the secondary is variable and exhibits extreme values of -810 +/- 150 and +680 +/- 190 G, implying a minimum surface dipole polar strength of 2850 +/- 500 G. In contrast, we derive an upper limit (3 sigma) to the primary\u27s surface magnetic field of 230 G. The combination of a strong magnetic field and rapid rotation leads us to conclude that the secondary hosts a centrifugal magnetosphere fed through a magnetically confined wind. We revisit the properties of the optical line profiles and X-ray emission - previously interpreted as a consequence of colliding stellar winds - in this context. We conclude that HD 47129 represents a heretofore unique stellar system - a close, massive binary with a rapidly rotating, magnetized component - that will be a rich target for further study

Bosonization and Fermion Liquids in Dimensions Greater Than One

(Revised, with postscript figures appended, corrections and added comments.) We develop and describe new approaches to the problem of interacting Fermions in spatial dimensions greater than one. These approaches are based on generalizations of powerful tools previously applied to problems in one spatial dimension. We begin with a review of one-dimensional interacting Fermions. We then introduce a simplified model in two spatial dimensions to study the role that spin and perfect nesting play in destabilizing Fermion liquids. The complicated functional renormalization group equations of the full problem are made tractable in our model by replacing the continuum of points that make up the closed Fermi line with four Fermi points. Despite this drastic approximation, the model exhibits physically reasonable behavior both at half-filling (where instabilities occur) and away from half-filling (where a Luttinger liquid arises). Next we implement the Bosonization of higher dimensional Fermi surfaces introduced by Luther and advocated most recently by Haldane. Bosonization incorporates the phase space and small-angle scattering .... (7 figures, appended as a postscript file at the end of the TeX file).Comment: 48 text pages, plain TeX, BUP-JBM-

Decrease in plasma miR-27a and miR-221 after concussion in Australian football players

Introduction: Sports-related concussion (SRC) is a common form of brain injury that lacks reliable methods to guide clinical decisions. MicroRNAs (miRNAs) can influence biological processes involved in SRC, and measurement of miRNAs in biological fluids may provide objective diagnostic and return to play/recovery biomarkers. Therefore, this prospective study investigated the temporal profile of circulating miRNA levels in concussed male and female athletes. Methods: Pre-season baseline blood samples were collected from amateur Australian rules football players (82 males, 45 females). Of these, 20 males and 8 females sustained an SRC during the subsequent season and underwent blood sampling at 2-, 6- and 13-days post-injury. A miRNA discovery Open Array was conducted on plasma to assess the expression of 754 known/validated miRNAs. miRNA target identified were further investigated with quantitative real-time PCR (qRT-PCR) in a validation study. Data pertaining to SRC symptoms, demographics, sporting history, education history and concussion history were also collected. Results: Discovery analysis identified 18 candidate miRNA. The consequent validation study found that plasma miR-221-3p levels were decreased at 6d and 13d, and that miR-27a-3p levels were decreased at 6d, when compared to baseline. Moreover, miR-27a and miR-221-3p levels were inversely correlated with SRC symptom severity. Conclusion: Circulating levels of miR-27a-3p and miR-221-3p were decreased in the sub-acute stages after SRC, and were inversely correlated with SRC symptom severity. Although further studies are required, these analyses have identified miRNA biomarker candidates of SRC severity and recovery that may one day assist in its clinical management

Dengue Virus Infection and Virus-Specific HLA-A2 Restricted Immune Responses in Humanized NOD-scid IL2rγnull Mice

BACKGROUND:The lack of a suitable animal model to study viral and immunological mechanisms of human dengue disease has been a deterrent to dengue research. METHODOLOGY/PRINCIPAL FINDINGS:We sought to establish an animal model for dengue virus (DENV) infection and immunity using non-obese diabetic/severe combined immunodeficiency interleukin-2 receptor gamma-chain knockout (NOD-scid IL2rgamma(null)) mice engrafted with human hematopoietic stem cells. Human CD45(+) cells in the bone marrow of engrafted mice were susceptible to in vitro infection using low passage clinical and established strains of DENV. Engrafted mice were infected with DENV type 2 by different routes and at multiple time points post infection, we detected DENV antigen and RNA in the sera, bone marrow, spleen and liver of infected engrafted mice. Anti-dengue IgM antibodies directed against the envelope protein of DENV peaked in the sera of mice at 1 week post infection. Human T cells that developed following engraftment of HLA-A2 transgenic NOD-scid IL2rgamma(null) mice with HLA-A2(+) human cord blood hematopoietic stem cells, were able to secrete IFN-gamma, IL-2 and TNF-alpha in response to stimulation with three previously identified A2 restricted dengue peptides NS4b 2353((111-119)), NS4b 2423((181-189)), and NS4a 2148((56-64)). CONCLUSIONS/SIGNIFICANCE:This is the first study to demonstrate infection of human cells and functional DENV-specific T cell responses in DENV-infected humanized mice. Overall, these mice should be a valuable tool to study the role of prior immunity on subsequent DENV infections

NGC 1624-2: A slowly rotating, X-ray luminous Of?cp star with an extraordinarily strong magnetic field

This paper presents a first observational investigation of the faint Of?cp star NGC 1624-2, yielding important new constraints on its spectral and physical characteristics, rotation, magnetic field strength, X-ray emission and magnetospheric properties. Modeling the spectrum and spectral energy distribution, we conclude that NGC 1624-2 is a main sequence star of mass M {\simeq} 30 M{\odot}, and infer an effective temperature of 35 {\pm} 2 kK and log g = 4.0 {\pm} 0.2. Based on an extensive time series of optical spectral observations we report significant variability of a large number of spectral lines, and infer a unique period of 157.99 {\pm} 0.94 d which we interpret as the rotational period of the star. We report the detection of a very strong - 5.35 {\pm} 0.5 kG - longitudinal magnetic field , coupled with probable Zeeman splitting of Stokes I profiles of metal lines confirming a surface field modulus of 14 {\pm} 1 kG, consistent with a surface dipole of polar strength >~ 20 kG. This is the largest magnetic field ever detected in an O-type star, and the first report of Zeeman splitting of Stokes I profiles in such an object. We also report the detection of reversed Stokes V profiles associated with weak, high-excitation emission lines of O iii, which we propose may form in the close magnetosphere of the star. We analyze archival Chandra ACIS-I X-ray data, inferring a very hard spectrum with an X-ray efficiency log Lx/Lbol = -6.4, a factor of 4 larger than the canonical value for O-type stars and comparable to that of the young magnetic O-type star {\theta}1 Ori C and other Of?p stars. Finally, we examine the probable magnetospheric properties of the star, reporting in particular very strong magnetic confinement of the stellar wind, with {\eta}* {\simeq} 1.5 {\times} 10^4, and a very large Alfven radius, RAlf = 11.4 R*.Comment: 17 pages, MNRAS accepted and in pres

Optimised Motion Tracking for Positron Emission Tomography Studies of Brain Function in Awake Rats

Positron emission tomography (PET) is a non-invasive molecular imaging technique using positron-emitting radioisotopes to study functional processes within the body. High resolution PET scanners designed for imaging rodents and non-human primates are now commonplace in preclinical research. Brain imaging in this context, with motion compensation, can potentially enhance the usefulness of PET by avoiding confounds due to anaesthetic drugs and enabling freely moving animals to be imaged during normal and evoked behaviours. Due to the frequent and rapid motion exhibited by alert, awake animals, optimal motion correction requires frequently sampled pose information and precise synchronisation of these data with events in the PET coincidence data stream. Motion measurements should also be as accurate as possible to avoid degrading the excellent spatial resolution provided by state-of-the-art scanners. Here we describe and validate methods for optimised motion tracking suited to the correction of motion in awake rats. A hardware based synchronisation approach is used to achieve temporal alignment of tracker and scanner data to within 10 ms. We explored the impact of motion tracker synchronisation error, pose sampling rate, rate of motion, and marker size on motion correction accuracy. With accurate synchronisation (<100 ms error), a sampling rate of >20 Hz, and a small head marker suitable for awake animal studies, excellent motion correction results were obtained in phantom studies with a variety of continuous motion patterns, including realistic rat motion (<5% bias in mean concentration). Feasibility of the approach was also demonstrated in an awake rat study. We conclude that motion tracking parameters needed for effective motion correction in preclinical brain imaging of awake rats are achievable in the laboratory setting. This could broaden the scope of animal experiments currently possible with PET

WNT signalling in prostate cancer

Genome sequencing and gene expression analyses of prostate tumours have highlighted the potential importance of genetic and epigenetic changes observed in WNT signalling pathway components in prostate tumours-particularly in the development of castration-resistant prostate cancer. WNT signalling is also important in the prostate tumour microenvironment, in which WNT proteins secreted by the tumour stroma promote resistance to therapy, and in prostate cancer stem or progenitor cells, in which WNT-β-catenin signals promote self-renewal or expansion. Preclinical studies have demonstrated the potential of inhibitors that target WNT receptor complexes at the cell membrane or that block the interaction of β-catenin with lymphoid enhancer-binding factor 1 and the androgen receptor, in preventing prostate cancer progression. Some WNT signalling inhibitors are in phase I trials, but they have yet to be tested in patients with prostate cancer