Estimating diet composition from scat analysis in otariid seals (Otariidae): is it reliable?

Analysis of teleost sagittal otoliths contained in scats has been widely used to determine the diet of seals. This method is based on the assumption that relative frequencies of otoliths in scats faithfully reflect those of fish in the diet. This assumption has rarely been tested experimentally. We compared the ratios of herring (Clupea harengus) to sprat (Sprattus sprattus) oto liths in faeces (output) of captive California sea lions (Zalophus calijornianus) and South American fur seals (Arctocephalus australis) with the ratios at feeding (input). Sea lions and fur seals showed no consistent differences in recovery rates and partial digestion of otoliths. Output ratios deviated only slightly from input ratios, the smaller sprat otoliths being underrepre sented in the output by 8%. Only about 40% of the otoliths fed to the seals were found in the scats. For both species partial digestion of otoliths led to a 16% underestimation of fish length and a 35% underestimation of fish mass.L'analyse des otolithes de tClCostCens contenus dans les fkces est une mCthode trks rCpandue de dktermination du rCgime alimentaire des phoques. Cette mCthode suppose que les frCquences relatives d'otolithes dans les fkces reflktent fidklement les frkquences relatives des poissons dans le rCgime. Cette prCmisse n'a pratiquement jamais fait l'objet de ~Crifications expkrimentales. Nous avons mesurC la proportion d'otolithes de harengs (Clupea harengus) et celle d'otolithes de sprats (Sprattus sprattus) dans les fkces (sortie) de Lions de mer californiens, Zalophus calijiornianus, et d'otaries a fourrure sud amkricaines, Arctocephalus australis, gardCs en captivitC, et comparC ces mesures aux proportions d'otolithes ingCrCs (entrCe). I1 n'y avait pas de diffkrence constante entre lions de mer et otaries et les taux de rCcupCration et de digestion partielle d'otolithes Ctaient les memes chez les deux espkces. Les proportions a la sortie diffkraient seulement un peu des proportions a I'entrCe et les otolithes des sprats, plus petits, Ctaient sous-reprCsentCs de 8% a la sortie. Seulement 40% environ des oto lithes ingCrCs ont CtC retrouvks a la sortie. La digestion partielle des otolithes chez les deux espkces entraine une sous-estima tion de la longueur des poissons consommCs, de I'ordre de 16%, et de la masse des poissons consommCs, de l'ordre de 35%.info:eu-repo/semantics/publishedVersio

Within-family environment and cross-fostering stress affect behavior and physiology in wild cavies (Cavia aperea)

Stability of personality traits is well-documented for a wide variety of animals. However, previous results also suggest that behavioral phenotypes are plastic during early ontogeny and can be adaptively shaped to the social environment. In cavies (Cavia aperea), it has already been documented that the size at birth relative to siblings (size rank) greatly influences various behavioral and physiological traits that last at least until independence. The aim of the current study was (1) to investigate if behavioral and physiological differences between pups of the same litter persist until after independence and influence development long-lasting, (2) to determine the potential plasticity in response to changes in the early within-family environment by cross-fostering pups either to the same, a lower, or a higher size rank in a foster-family. We measured three behavioral traits (number of interactions with a novel object, distance moved in an open field, struggle docility) and two physiological traits (resting metabolic rate and basal cortisol levels). We predicted that cross-fostering into a litter where pups occupy the same size rank would not change the expression of traits. Cross-fostering to a different size rank should not influence the expression of traits if repeatability measures indicate low plasticity. Alternatively, if the traits are plastic, animals should adjust trait expression to fit with the size rank occupied in the foster litter. Initial differences in struggle docility, distance moved in an open field and in baseline cortisol concentration between pups of different size-ranks did not remain stable beyond independence. In addition, we found remarkable plasticity of the measured traits in response to cross-fostering to the same, a smaller or larger size-rank, suggesting that differences between pups are more the result of social constraints leading to adaptive shaping of individual phenotypes within a family. We also found a significant influence of the cross-fostering procedure itself. Cross-fostered individuals were less bold, grew slower and showed elevated resting metabolic rates. This finding suggests a cautious interpretation of previous cross-fostering studies and stresses the need for proper control groups to reliably separate the effect of cross-fostering per se from those induced by an experimental treatment

Reproduction affects immune defenses in the guinea pig even under ad libitum food

Reproduction is one of the costliest processes in the life of an animal. Life history theory assumes that when resources are limiting allocation to reproduction will reduce allocation to other essential processes thereby inducing costs of reproduction. The immune system is vital for survival. If reproduction reduces investment in immune function, this could increase the risk of disease, morbidity and mortality. We here test in the guinea pig, if even under ad libitum food conditions, pregnancy and lactation reduce the activity of the adaptive and innate immune system compared to the reaction of non-reproducing animals. In response to a challenge with keyhole limpet haemocyanin the antibody-mediated adaptive immunity during (pregnancy and) lactation was reduced. Pregnant and lactating females showed higher levels of bacterial killing activity, an integrated measure of innate immunity, than non-reproducing females. However, two major effectors of the innate immunity, the natural antibody and the complement of pregnant and lactating females showed lower levels than in non-reproducing females. Pregnant and lactating females did not differ significantly in the expressed levels of innate immunity. Our results indicate that changes in the immune response during reproduction are physiological adjustments to predictable allocation problems, because they happen even under ad libitum food availability

The spatial ecology of invasive feral cats Felis catus on San Cristóbal, Galápagos: first insights from GPS collars

AbstractThe dangers posed by invasive species for endemic island wildlife are well recognised. Introduced domestic cats (Felis catus) represent a significant threat to several endemic species of the Galápagos archipelago—including hatchling marine iguanas (Amblyrhynchus cristatus) and potentially green turtles (Chelonia mydas)—yet little is known about their spatial ecology and habitat use on these islands. Here, we describe a pilot study using GPS collars to track the movements of three feral cats at a site of conservation interest on San Cristóbal Island. Based on 175 days of GPS data, we undertook spatial analyses to ascertain home ranges, and to investigate the overlap of ranges between the cats and potential prey species. Average home range was 1.27 km2 (1.12–1.46 km2), which—though small for feral cats—is in keeping with previous findings in Galápagos. We found the cats did use the habitat of a small marine iguana population but did not change their spatial habits before and after iguana hatchlings appeared. Changes over time in the daily movements of one cat indicated a possible response of the individual to the presence of hatchling green turtles (Chelonia mydas); though the data here are insufficient to show whether the cat was hunting these hatchlings. We recommend similar work be undertaken in areas with larger marine iguana populations, where hatchlings could represent a potentially important food source for invasive feral cats, as well as further work to determine the threat posed by cats to turtle hatchlings in Galápagos.</jats:p

The spatial ecology of invasive feral cats Felis catus on San Cristóbal, Galápagos: first insights from GPS collars

Abstract: The dangers posed by invasive species for endemic island wildlife are well recognised. Introduced domestic cats (Felis catus) represent a significant threat to several endemic species of the Galápagos archipelago—including hatchling marine iguanas (Amblyrhynchus cristatus) and potentially green turtles (Chelonia mydas)—yet little is known about their spatial ecology and habitat use on these islands. Here, we describe a pilot study using GPS collars to track the movements of three feral cats at a site of conservation interest on San Cristóbal Island. Based on 175 days of GPS data, we undertook spatial analyses to ascertain home ranges, and to investigate the overlap of ranges between the cats and potential prey species. Average home range was 1.27 km2 (1.12–1.46 km2), which—though small for feral cats—is in keeping with previous findings in Galápagos. We found the cats did use the habitat of a small marine iguana population but did not change their spatial habits before and after iguana hatchlings appeared. Changes over time in the daily movements of one cat indicated a possible response of the individual to the presence of hatchling green turtles (Chelonia mydas); though the data here are insufficient to show whether the cat was hunting these hatchlings. We recommend similar work be undertaken in areas with larger marine iguana populations, where hatchlings could represent a potentially important food source for invasive feral cats, as well as further work to determine the threat posed by cats to turtle hatchlings in Galápagos

Scents of Adolescence: The Maturation of the Olfactory Phenotype in a Free-Ranging Mammal

Olfaction is an important sensory modality for mate recognition in many mammal species. Odorants provide information about the health status, genotype, dominance status and/or reproductive status. How and when odor profiles change during sexual maturation is, however often unclear, particularly in free-ranging mammals. Here, we investigated whether the wing sac odorant of male greater sac-winged bats (Saccopteryx bilineata, Emballonuridae) differs between young and adults, and thus offers information about sexual maturity to potential mating partners. Using gas chromatography – mass spectrometry, we found differences in the odorants of young and adult males prior and during, but not after the mating period. The wing sac odorant of adult males consists of several substances, such as Pyrocoll, 2,6,10-trimethyl-3-oxo-6,10-dodecadienolide, and a so far unidentified substance; all being absent in the odor profiles of juveniles prior to the mating season. During the mating season, these substances are present in most of the juvenile odorants, but still at lower quantities compared to the wing sac odorants of adults. These results suggest that the wing sac odorant of males encodes information about age and/or sexual maturity. Although female S. bilineata start to reproduce at the age of half a year, most males of the same age postpone the sexual maturation of their olfactory phenotype until after the first mating season

Genetic Impact of a Severe El Niño Event on Galápagos Marine Iguanas (Amblyrhynchus cristatus)

The El Niño-Southern Oscillation (ENSO) is a major source of climatic disturbance, impacting the dynamics of ecosystems worldwide. Recent models predict that human-generated rises in green-house gas levels will cause an increase in the strength and frequency of El Niño warming events in the next several decades, highlighting the need to understand the potential biological consequences of increased ENSO activity. Studies have focused on the ecological and demographic implications of El Niño in a range of organisms, but there have been few systematic attempts to measure the impact of these processes on genetic diversity in populations. Here, we evaluate whether the 1997–1998 El Niño altered the genetic composition of Galápagos marine iguana populations from eleven islands, some of which experienced mortality rates of up to 90% as a result of El Niño warming. Specifically, we measured the temporal variation in microsatellite allele frequencies and mitochondrial DNA diversity (mtDNA) in samples collected before (1991/1993) and after (2004) the El Niño event. Based on microsatellite data, only one island (Marchena) showed signatures of a genetic bottleneck, where the harmonic mean of the effective population size (Ne) was estimated to be less than 50 individuals during the period between samplings. Substantial decreases in mtDNA variation between time points were observed in populations from just two islands (Marchena and Genovesa). Our results suggests that, for the majority of islands, a single, intense El Niño event did not reduce marine iguana populations to the point where substantial neutral genetic diversity was lost. In the case of Marchena, simultaneous changes to both nuclear and mitochondrial DNA variation may also be the result of a volcanic eruption on the island in 1991. Therefore, studies that seek to evaluate the genetic impact of El Niño must also consider the confounding or potentially synergistic effect of other environmental and biological forces shaping populations

The Cost of Male Aggression and Polygyny in California Sea Lions (Zalophus californianus)

In polygynous mating systems, males often increase their fecundity via aggressive defense of mates and/or resources necessary for successful mating. Here we show that both male and female reproductive behavior during the breeding season (June–August) affect female fecundity, a vital rate that is an important determinant of population growth rate and viability. By using 4 years of data on behavior and demography of California sea lions (Zalophus californianus), we found that male behavior and spatial dynamics—aggression and territory size—are significantly related to female fecundity. Higher rates of male aggression and larger territory sizes were associated with lower estimates of female fecundity within the same year. Female aggression was significantly and positively related to fecundity both within the same year as the behavior was measured and in the following year. These results indicate that while male aggression and defense of territories may increase male fecundity, such interactions may cause a reduction in the overall population growth rate by lowering female fecundity. Females may attempt to offset male-related reductions in female fecundity by increasing their own aggression—perhaps to defend pups from incidental injury or mortality. Thus in polygynous mating systems, male aggression may increase male fitness at the cost of female fitness and overall population viability

Climate Driven Egg and Hatchling Mortality Threatens Survival of Eastern Pacific Leatherback Turtles

Egg-burying reptiles need relatively stable temperature and humidity in the substrate surrounding their eggs for successful development and hatchling emergence. Here we show that egg and hatchling mortality of leatherback turtles (Dermochelys coriacea) in northwest Costa Rica were affected by climatic variability (precipitation and air temperature) driven by the El Niño Southern Oscillation (ENSO). Drier and warmer conditions associated with El Niño increased egg and hatchling mortality. The fourth assessment report of the Intergovernmental Panel on Climate Change (IPCC) projects a warming and drying in Central America and other regions of the World, under the SRES A2 development scenario. Using projections from an ensemble of global climate models contributed to the IPCC report, we project that egg and hatchling survival will rapidly decline in the region over the next 100 years by ∼50–60%, due to warming and drying in northwestern Costa Rica, threatening the survival of leatherback turtles. Warming and drying trends may also threaten the survival of sea turtles in other areas affected by similar climate changes