17 research outputs found

    An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology

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    Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins greater than 150 kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances in imaging hardware, image processing software, and improvements in sample preparation, leading to growing number of researchers using cryo-EM as a research tool. However, cryo-EM is still a rapidly growing field, with unique challenges. Here, we summarise considerations for imaging of a range of specimens from macromolecular complexes to cells using EM

    Zur sacculusbedingten Bulbusthrombose

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    Über Folgen unvollständiger Sanierung von Verletzungen im Bereich der pneumatischen Räume des Kopfes

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