Ignition and combustion phenomena in Diesel engines

Evidences were found that neither gasification nor vaporization of the injected fuel occurs before ignition; also that the hydrogen coefficient has no significance. However the knowledge of the ignition point and of the "time lag" is important. After ignition, the combustion proceeds in a series of reactions, the last of which at least are now known

A Twin-Embryo Abnormality in Maize

A mutant strain of corn obtained by ultra violet treatment has twin growing points in the embryo. The flattened emerging coleoptile is a continuous sheath, containing two distinct stem apices, each of which lays down a series of leaf primordia. Each growing point becomes encircled by its laterally overlapping young leaves. The procambium strands of the two young axes converge into the stele of the mesocotyl (first internode), which is common to the two potential stems

Perceived Influence and Response of River Users to Invasive Bighead and Silver Carp in the Illinois River

Two United States federally injurious fishes, bighead(Hypophthalmichthys nobilis)and silver carp(H. molitrix),are established in the Mississippi River Basinand have the potential tocolonize the Laurentian Great Lakes. Given this imminentthreat, a critical need for stakeholders is an understanding ofthe potential social influences of this invasion oncommunities that rely upon the ecosystems bighead and silvercarp now inhabit; influences so far unstudied. Using intercept surveys (n= 469), we tested forthe potential social influencesof bighead and silvercarp in four river communities;three in which they haveestablishedpopulations(test, Illinois River, Illinois) and one in which they have not(control, Mississippi River, Iowa). Reported principal differences between test and control sites were observed for recreational fishing, aestheticappreciation of the river, and experience with bighead and silvercarp. Our exploratory results indicate that bighead and silvercarp may have a negative influence on river usage.Illinois Natural History Surveyunpublishednot peer reviewedOpe

A novel technique for selective NF-kappa B inhibition in Kupffer cells: contrary effects in fulminant hepatitis and ischaemia-reperfusion.

Background and aims: The transcription factor nuclear factor kappa B (NF-kB) has risen as a promising target for anti-inflammatory therapeutics. In the liver, however, NFkB inhibition mediates both damaging and protective effects. The outcome is deemed to depend on the liver cell type addressed. Recent gene knock-out studies focused on the role of NF-kB in hepatocytes, whereas the role of NF-kB in Kupffer cells has not yet been investigated in vivo. Here we present a novel approach, which may be suitable for clinical application, to selectively target NF-kB in Kupffer cells and analyse the effects in experimental models of liver injury. Methods: NF-kB inhibiting decoy oligodeoxynucleotides were loaded upon gelatin nanoparticles (D-NPs) and their in vivo distribution was determined by confocal microscopy. Liver damage, NF-kB activity, cytokine levels and apoptotic protein expression were evaluated after lipopolysaccharide (LPS), D-galactosamine (GalN)/LPS, or concanavalin A (ConA) challenge and partial warm ischaemia and subsequent reperfusion, respectively. Results: D-NPs were selectively taken up by Kupffer cells and inhibited NF-kB activation. Inhibition of NF-kB in Kupffer cells improved survival and reduced liver injury after GalN/LPS as well as after ConA challenge. While anti-apoptotic protein expression in liver tissue was not reduced, pro-apoptotic players such as cJun N-terminal kinase (JNK) were inhibited. In contrast, selective inhibition of NF-kB augmented reperfusion injury. Conclusions: NF-kB inhibiting decoy oligodeoxynucleotide- loaded gelatin nanoparticles is a novel tool to selectively inhibit NF-kB activation in Kupffer cells in vivo. Thus, liver injury can be reduced in experimental fulminant hepatitis, but increased at ischaemia–reperfusion

Lixiviação de nutrientes em substrato enriquecido com biochar.

Resumo

Estimation and correction of geometric distortions in side-scan sonar images

Submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at the Massachusetts Institute of Technology and the Woods Hole Oceanographic Institution March 1990This thesis introduces a new procedure for the enhancement of acoustic images of the bottom of the sea produced by side-scan sonars. Specifically, it addresses the problem of estimating and correcting geometric distortions frequently observed in such images as a consequence of motion instabilities of the sonar array. This procedure estimates the geometric distortions from the image itself, without requiring any navigational or attitude measurements. A mathematical model for the distortions is derived from the geometry of the problem, and is applied to estimates of the local degree of geometric distortion obtained by cross-correlating segments of adjacent lines of the image. The model parameters are then recursively estimated through deterministic least-squares estimation. An alternative approach based on adaptive Kalman filtering is also proposed, providing a natural framework in which a priori information about the array dynamics may be easily incorporated. The estimates of the parameters of the distortion model are used to rectify the image, and may also be used for estimating the attitude parameters of the array. A simulation is employed to evaluate the effectiveness of this technique and examples of its application to high-resolution side-scan sonar images are provided.This work was produced under sponsorship of the Conselho Nacional de Desenvolvimento Cient{jico e Tecnol6gico (CNPq), an agency of the Government of the Federative Republic of Brazil, and was supported in part by the Defense Advanced Research Projects Agency monitored by the Office of Naval Research under Grant No. N00014-89-J-1489, in part by the National Science Foundation under Grant No. MIP 87-14969, and in part by Sanders Associates, Incorporated

Revisiting the Zingiberales: Using Multiplexed Exon Capture to Resolve Ancient and Recent Phylogenetic Splits in a Charismatic Plant Lineage

The Zingiberales are an iconic order of monocotyledonous plants comprising eight families with distinctive and diverse floral morphologies and representing an important ecological element of tropical and subtropical forests. While the eight families are demonstrated to be monophyletic, phylogenetic relationships among these families remain unresolved. Neither combined morphological and molecular studies nor recent attempts to resolve family relationships using sequence data from whole plastomes has resulted in a well-supported, family-level phylogenetic hypothesis of relationships. Here we approach this challenge by leveraging the complete genome of one member of the order, Musa acuminata, together with transcriptome information from each of the other seven families to design a set of nuclear loci that can be enriched from highly divergent taxa with a single array-based capture of indexed genomic DNA. A total of 494 exons from 418 nuclear genes were captured for 53 ingroup taxa. The entire plastid genome was also captured for the same 53 taxa. Of the total genes captured, 308 nuclear and 68 plastid genes were used for phylogenetic estimation. The concatenated plastid and nuclear dataset supports the position of Musaceae as sister to the remaining seven families. Moreover, the combined dataset recovers known intra- and inter-family phylogenetic relationships with generally high bootstrap support. This is a flexible and cost effective method that gives the broader plant biology community a tool for generating phylogenomic scale sequence data in non-model systems at varying evolutionary depths

Heritable Characters in Maize: Accessory Blade

A mutant character in maize brings about the formation of ridge and blade-like outgrowths on the surfaces of leaves. These accessory laminae, which are present on the partially developed leaves in the dormant embryo, arise by re-activation of vertical zones on the immature leaf

Compromised Bone Healing in Aged Rats Is Associated With Impaired M2 Macrophage Function

Fracture repair is initiated by a multitude of immune cells and induction of an inflammatory cascade. Alterations in the early healing response due to an aged adaptive immune system leads to impaired bone repair, delayed healing or even formation of non-union. However, immuno-senescence is not limited to the adaptive immunity, but is also described for macrophages, main effector cells from the innate immune system. Beside regulation of pro- and anti-inflammatory signaling, macrophages contribute to angiogenesis and granulation tissue maturation. Thus, it seems likely that an altered macrophage function due to aging may affect bone repair at various stages and contribute to age related deficiencies in bone regeneration. To prove this hypothesis, we analyzed the expression of macrophage markers and angiogenic factors in the early bone hematoma derived from young and aged osteotomized Spraque Dawley rats. We detected an overall reduced expression of the monocyte/pan-macrophage markers CD14 and CD68 in aged rats. Furthermore, the analysis revealed an impaired expression of anti-inflammatory M2 macrophage markers in hematoma from aged animals that was connected to a diminished revascularization of the bone callus. To verify that the age related disturbed bone regeneration was due to a compromised macrophage function, CD14+ macrophage precursors were transplanted locally into the osteotomy gap of aged rats. Transplantation rescued bone regeneration partially after 6 weeks, demonstrated by a significantly induced deposition of new bone tissue, reduced fibrosis and significantly improved callus vascularization

Experimental Control and Characterization of Autophagy in Drosophila

Insects such as the fruit fly Drosophila melanogaster, which fundamentally reorganize their body plan during metamorphosis, make extensive use of autophagy for their normal development and physiology. In the fruit fly, the hepatic/adipose organ known as the fat body accumulates nutrient stores during the larval feeding stage. Upon entering metamorphosis, as well as in response to starvation, these nutrients are mobilized through a massive induction of autophagy, providing support to other tissues and organs during periods of nutrient deprivation. High levels of autophagy are also observed in larval tissues destined for elimination, such as the salivary glands and larval gut. Drosophila is emerging as an important system for studying the functions and regulation of autophagy in an in vivo setting. In this chapter we describe reagents and methods for monitoring autophagy in Drosophila, focusing on the larval fat body. We also describe methods for experimentally activating and inhibiting autophagy in this system and discuss the potential for genetic analysis in Drosophila to identify novel genes involved in autophagy