Biopharmacy practices virtualization: dissolution test of several dosage forms

En años anteriores, la Unidad de Biofarmacia y Farmacocinética de la Universidad de Barcelona, ha estado inmersa en la virtualización de las prácticas de la asignatura de cara a que en un futuro próximo puedan realizarse con mayor autonomía. En un trabajo previo se llevó a cabo la virtualización de la parte referente a las prácticas de farmacocinética. Prosiguiendo con esta labor, ahora se presenta la virtualización de las prácticas de biofarmacia. Debido a limitaciones temporales y económicas, el estudiante de grado, realiza la práctica del ensayo de disolución de comprimidos de forma simplificada. Para que el alumno pueda ver como se realizan los ensayos de disolución de forma farmacéuticas sólidas según las directrices internacionales que realizará en su vida laboral, hemos confeccionado una serie de videos explicativos de dicha tarea, y se ha ampliado con material audiovisual sobre ensayos de disolución de formas sólidas y de parches transdérmicos, los cuales no se realizan de forma rutinaria durante las prácticas de la licenciatura. Dicho material audiovisual estará a disposición de los alumnos en la plataforma virtual moodle para que pueda ser consultado cuando sea necesario.Previously, the Biopharmaceutical and Pharmacokinetic Unit of the University of Barcelona had been immersed in the practices’ virtualization of the grade subject, looking for an improvement of student’s autonomy in a foreseeable future. The virtualization of the pharmacokinetic practices was made previously, and now we are presenting the virtualization of the biopharmaceutical section. Due to the economic and time limitations, students only carried out the tablet dissolution assay in a simplified way. The aim of this guide is to show how to perform a different dissolution test by visualizing a video, which teaches the students, how to proceed according to the international guidelines. Students will be able to study it in the virtual platform moodle, whenever it may be necessary

New nanotechnologies for the treatment and repair of skin burns infections

Burn wounds are highly debilitating injuries, with significant morbidity and mortality rates worldwide. In association with the damage of the skin integrity, the risk of infection is increased, posing an obstacle to healing and potentially leading to sepsis. Another limitation against healing is associated with antibiotic resistance mainly due to the use of systemic antibiotics for the treatment of localized infections. Nanotechnology has been successful in finding strategies to incorporate antibiotics in nanoparticles for the treatment of local wounds, thereby avoiding the systemic exposure to the drug. This review focuses on the most recent advances on the use of nanoparticles in wound dressing formulations and in tissue engineering for the treatment of burn wound infections.The authors would like to thank the financial support received from Portuguese Science and Technology Foundation (FCT/MCT) and from European Funds (PRODER/COMPETE) under the project references M-ERA-NET/0004/2015-PAIRED and UIDB/04469/2020, co-financed by FEDER, under the Partnership Agreement PT2020.info:eu-repo/semantics/publishedVersio

Adaptive Robotic Control Driven by a Versatile Spiking Cerebellar Network

The cerebellum is involved in a large number of different neural processes, especially in associative learning and in fine motor control. To develop a comprehensive theory of sensorimotor learning and control, it is crucial to determine the neural basis of coding and plasticity embedded into the cerebellar neural circuit and how they are translated into behavioral outcomes in learning paradigms. Learning has to be inferred from the interaction of an embodied system with its real environment, and the same cerebellar principles derived from cell physiology have to be able to drive a variety of tasks of different nature, calling for complex timing and movement patterns. We have coupled a realistic cerebellar spiking neural network (SNN) with a real robot and challenged it in multiple diverse sensorimotor tasks. Encoding and decoding strategies based on neuronal firing rates were applied. Adaptive motor control protocols with acquisition and extinction phases have been designed and tested, including an associative Pavlovian task (Eye blinking classical conditioning), a vestibulo-ocular task and a perturbed arm reaching task operating in closed-loop. The SNN processed in real-time mossy fiber inputs as arbitrary contextual signals, irrespective of whether they conveyed a tone, a vestibular stimulus or the position of a limb. A bidirectional long-term plasticity rule implemented at parallel fibers-Purkinje cell synapses modulated the output activity in the deep cerebellar nuclei. In all tasks, the neurorobot learned to adjust timing and gain of the motor responses by tuning its output discharge. It succeeded in reproducing how human biological systems acquire, extinguish and express knowledge of a noisy and changing world. By varying stimuli and perturbations patterns, real-time control robustness and generalizability were validated. The implicit spiking dynamics of the cerebellar model fulfill timing, prediction and learning functions.European Union (Human Brain Project) REALNET FP7-ICT270434 CEREBNET FP7-ITN238686 HBP-60410

Modelling human choices: MADeM and decision‑making

Research supported by FAPESP 2015/50122-0 and DFG-GRTK 1740/2. RP and AR are also part of the Research, Innovation and Dissemination Center for Neuromathematics FAPESP grant (2013/07699-0). RP is supported by a FAPESP scholarship (2013/25667-8). ACR is partially supported by a CNPq fellowship (grant 306251/2014-0)

Self-learning of Biopharmacy and Pharmacokinetic computer software

Uno de los pilares de la Biofarmacia y Farmacocinética es el tratamiento de datos, para lo que resulta imprescindible el conocimiento de programas informáticos estadísticos y de regresión no lineal. Estos son ampliamente usados en nuestra Unidad y forman parte de las destrezas prácticas que el alumno debe adquirir. Debido a las dificultades presentadas por los alumnos en la comprensión de ambas disciplinas, nos hemos planteado realizar una guía de apoyo virtual, sobre el uso del programa de regresión no lineal WinNonLin®. Dicho material estará a disposición del alumno a través de la plataforma virtual moodle para que pueda ser consultado antes de realizar las prácticas de la asignatura. Para valorar la utilidad de este material de apoyo como herramienta TIC, se han realizado una encuesta a profesores y alumnos vinculados a la Unidad, que son los usuarios principales de estas herramientas informáticas. Los resultados de las encuestas reflejaron su agrado y facilidad de compresión, de tal forma que para el próximo curso 2011-2012 se colgará en moodle para todos los alumnos de grado.One of the most important tasks in the biopharmaceutical and pharmacokinetics area is data management, being the statistical and non linear regression software widely used. Those IT skills should be acquired by students in practical lessons, but due to the comprehension difficulties showed, the Biopharmaceutical and Pharmacokinetics Unit of University of Barcelona elaborated a virtual guidance about the use of WinNonLin®, non-linear regression software. This guidance will be used to solve student’s queries by the virtual campus Moodle, before the attending sessions. The main software users, professors and internal students, were polled about the usefulness of this guide. The survey conclusions emphasized its easy understanding and its utility, therefore all students, who were inscribed in Biopharmaceutics and Pharmacokinetic subject, will have access to this guidance next term

Cirugía de la catarata: de la extracción extracapsular a la facoemulsificación : evolución y resultados obtenidos por un residente de tercer año

Purpose: To analyze the results obtained in the cataract surgery by means of facoemulsification carried out by a M.I.R. of third year with a surgeon tutor. This results are compared with those obtained by means of cataract extracapsular extraction by the same M.I.R. in second year. Material and methods: It¿s carried out a retrospective study of 40 surgeries of cataract: 20 carried out by means of cataract extracapsular extraction and 20 by facoemulsification. The studied parameters have been classified in presurgical, surgical and postsurgical. Results: The visual acuity corrected after two months of the surgery was of 0.5 or superior in 90% of the total of the operated eyes and in 100% of those without associate pathology. The smallest surgical complications (zonular fibres rhexis, IOL turn, iris injury) it was 20% in EEC and 20% in FEC. The biggest percentage of surgical complications (vitreorrhage) it was 5% in EEC and 10% FEC. The postsurgical complications in the EEC is related with the suture (30%) and with the capsular opacification (20%). The postsurgical complications in FEC is related with the capsular opacificación (5%). Conclusions: The study shows an acceptable rate of complications, not having these functional repercussion.Objetivos: Analizar los resultados obtenidos en la cirugía de la catarata mediante facoemulsificación (FEC) realizadas por un M.I.R. de tercer año con un facultativo tutor. Se comparan dichos resultados con los obtenidos mediante extracción extracapsular (EEC) por el mismo M.I.R. en segundo año. Material y métodos: Se realiza un estudio retrospectivo de 40 cirugías de cataratas: 20 realizadas mediante técnica extracapsular y 20 por facoemulsificación. Los parámetros estudiados han sido clasificados en prequirúrgicos, quirúrgicos y postquirúrgicos. Resultados: La agudeza visual corregida a los dos meses de la cirugía fue de 0.5 o superior en el 90% del total de los ojos operados y en el 100 % de aquellos sin patología asociada. Las complicaciones quirúrgicas menores (desinserción zonular, volteo de LIO, laceración iridiana) fueron del 20 % en EEC y del 20 % en FEC. El porcentaje de complicaciones quirúrgicas mayores (vitreorragia) fue del 5 % en EEC y del 10 % en FEC. Las complicaciones postquirúrgicas en la EEC se relacionan con la sutura (30 %) y con la opacificación capsular (20 %). Las complicaciones postquirúrgicas en la FEC más importantes se relacionan con la opacificación capsular (5 %). Conclusiones: El estudio muestra una tasa de complicaciones aceptable, no teniendo éstas repercusión funcional

Solución para resección endoscópica

Número de publicación: 2621877Número de solicitud: 20160030003La presente invención se refiere a una solución farmacéutica acuosa que comprende carboximetilcelulosa, preferiblemente ácido hialurónico, y carboximetilcelulosa. Más específicamente, hace referencia a una solución farmacéutica acuosa que comprende carboximetilcelulosa 0,005%-2%, preferiblemente donde dicha solución farmacéutica comprende: ácido hialurónico 0,0001%-5% y carboximetilcelulosa 0,005%-2%. Asimismo, hace referencia al uso de dicha solución farmacéutica acuosa en la elaboración de un medicamento para el tratamiento quirúrgico mediante resección endoscópica, por ejemplo, la resección de pólipos y/o tumores en la mucosa gastrointestinal.Universidad de GranadaAgencia Pública Empresarial Sanitaria Hospital de Ponient

Optimization of nimesulide-loaded solid lipid nanoparticles (SLN) by factorial design, release profile and cytotoxicity in human Colon adenocarcinoma cell line

The aim of this work is development of a nontoxic, long-term stable solid lipid nanoparticles (SLN) formulation for the loading of Nimesulide (NiM) by a 22 factorial design. The optimized formulation was composed of 10wt% of glyceryl behenate and 2.5wt% of poloxamer 188. Immediately after production, Z-Ave of NiM-SLN was 166.1±0.114nm, with a polydispersity index (PI) of 0.171±0051 and zeta potential nearly neutral (3.10±0.166mV). A slight increase of Z-Ave was recorded for NiM-SLN stored at 25°C for a period of 15days, whereas at 4°C particles kept size within similar range. Long-term stability was monitored using TurbiscanLab®, showing a high stability of the nanoparticles with variations in the backscattering profiles below 10%. The release profile of NiM-SLN followed a sustained pattern with ca. 30% of drug released up to 24h. Empty-SLN and NiM-SLN were nontoxic after exposing Caco-2 cells to the highest concentration (100g/mL) up to 48hours (cell viability higher than 80%). NiM-SLN were lyophilized using different cryoprotectants, producing particles of 463.1±36.63nm (PI 0.491±0.027) with 5% trehalose. Solid character of NiM-SLN was confirmed by DSC, recording a recrystallization index of 83% for NiM-SLN and of 74% for lyophilized SLN.This work was financed through the projects M-ERA-NET/0004/2015-PAIRED, receiving financial support from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, and co-financed by FEDER, under the Partnership Agreement PT2020. This work was also supported by the Spanish Ministry of Science and Innovation (MAT 2014-59134-R projects; 2014SGR-1023).info:eu-repo/semantics/publishedVersio