Resistance to eyespot (Pseudocercosporella herpotricoides) and distribution of biochemical markers in hexaploid lines derived from double cross (Triticum turgidum x Aegilops ventricosa) x T. aestivum

There are not good intraspecific sources of resistance to the eyespot disea se of wheat, aaused by Cercosporella herpotrichoides Fvon . The -ínterspecifia transfer of genes for resistanoe from Aegitops ventricosa into hexaploid wheat has been only partially achieved, because the degree of resistanoe attained is not as high as that of the donor. We report here on the transfer of resistanoe in a double oross (Triticum turgidum var. rubroatrwv H-1-1 x Ae.ventricosa AP-D x T.aestivum cv. Almatense H-10-15. The high level of resistanoe in a high proportion of the lines strongly suggests a simple genetic control for this oharacter (possibly by one major gene). The gene(s) responsible for resistanoe in the selected lines must be associa ted with the D genome of Aegilops ventricosa on the basis of a detailed study of the distribution of biochemioal markers in the H-93 lines. These results do not exelude that genes with similar effeets might be looated in the M° genome

Mimotope mapping as a complementary strategy to define allergen IgE-epitopes: peach Pru p 3 allergen as a model.

Lipid transfer proteins (LTPs) are the major allergens of Rosaceae fruits in the Mediterranean area. Pru p 3, the LTP and major allergen of peach, is a suitable model for studying food allergy and amino acid sequences related with its IgE-binding capacity. In this work, we sought to map IgE mimotopes on the structure of Pru p 3, using the combination of a random peptide phage display library and a three-dimensional modelling approach. Pru p 3-specific IgE was purified from 2 different pools of sera from peach allergic patients grouped by symptoms (OAS-pool or SYS-pool), and used for screening of a random dodecapeptide phage display library. Positive clones were further confirmed by ELISA assays testing individual sera from each pool. Three-dimensional modelling allowed location of mimotopes based on analysis of electrostatic properties and solvent exposure of the Pru p 3 surface. Twenty-one phage clones were selected using Pru p 3-specific IgE, 9 of which were chosen using OAS-specific IgE while the other 12 were selected with systemic-specific IgE. Peptide alignments revealed consensus sequences for each pool: L37 R39 T40 P42 D43 R44 A46 P70 S76 P78 Y79 for OAS-IgE, and N35 N36 L37 R39 T40 D43 A46 S76 I77 P78 for systemic-IgE. These 2 consensus sequences were mapped on the same surface of Pru p 3, corresponding to the helix 2-loop-helix 3 region and part of the non-structured C-terminal coil. Thus, 2 relevant conformational IgE-binding regions of Pru p 3 were identified using a random peptide phage display library. Mimotopes can be used to study the interaction between allergens and IgE, and to accelerate the process to design new vaccines and new immunotherapy strategie

Study of the influence of the needle lift on the internal flow and cavitationphenomenon in diesel injector nozzles by CFD using RANS methods

It is well known that cavitation phenomenon in diesel injector nozzles has a strong influence on the internal flow during the injection process and spray development. However, its influence on the flow during needle opening and closing remains still unclear due to the huge difficulties related to performing experiments at partial needle lifts. In this paper, an extended computational study has been performed in a multi-hole nozzle modeling 10 different fixed needle lifts. The internal flow has been modeled with a continuum nozzle flow model that considers the cavitating flow as a homogeneous mixture of liquid and vapour. Due to high Reynolds numbers, turbulence effects have been taken into account by RANS methods using a RNG k e model. Firstly, the code has been validated against experimental data at full needle lift conditions in terms of mass flow, momentum flux and effective velocity, showing a fairly good agreement with experimental results. Once the code has been validated, it has been possible to study in depth the internal nozzle flow and its characteristics at the outlet at different partial needle lifts. Nevertheless, not only the main flow features have been explained, but also the cavitation appearance and the turbulence development, which present huge differences between the different needle lifts simulated.The authors wish to acknowledge the Generalitat Valenciana for the financial support through the project GVA PROMETEO CMT 2010 (reference code: GR001/2009/00167539).Salvador, FJ.; Martínez López, J.; Caballer Fernández, M.; Alfonso Laguna, CD. (2013). Study of the influence of the needle lift on the internal flow and cavitationphenomenon in diesel injector nozzles by CFD using RANS methods. Energy Conversion and Management. 66:246-256. https://doi.org/10.1016/j.enconman.2012.10.0112462566

Continuous multi-criteria methods for crop and soil conservation planning on La Colacha (Río Cuarto, Province of Cordoba, Argentina)

Agro-areas of Arroyos Menores (La Colacha) west and south of Rand south of R?o Cuarto (Prov. of Cordoba, Argentina) basins are very fertile but have high soil loses. Extreme rain events, inundations and other severe erosions forming gullies demand urgently actions in this area to avoid soil degradation and erosion supporting good levels of agro production. The authors first improved hydrologic data on La Colacha, evaluated the systems of soil uses and actions that could be recommended considering the relevant aspects of the study area and applied decision support systems (DSS) with mathematic tools for planning of defences and uses of soils in these areas. These were conducted here using multi-criteria models, in multi-criteria decision making (MCDM); first of discrete MCDM to chose among global types of use of soils, and then of continuous MCDM to evaluate and optimize combined actions, including repartition of soil use and the necessary levels of works for soil conservation and for hydraulic management to conserve against erosion these basins. Relatively global solutions for La Colacha area have been defined and were optimised by Linear Programming in Goal Programming forms that are presented as Weighted or Lexicographic Goal Programming and as Compromise Programming. The decision methods used are described, indicating algorithms used, and examples for some representative scenarios on La Colacha area are given

Estudio de los efectos de algunos psicotropos en el test "Open Field"

Por medio del test "opcn-field" se ha analizado el comportamiento de ratas tratadas con diversos psicotropos ncurolépt icos. antidcpresivos, tranquilizntes, barbitú ricos, psicoana l~pt icos) y asociaciones, entre las que figura la de un IMAO (nialamida) y 5-hidroxitriptofano, precusor de la serotonina cerebral. De los resultados obtenidos se deduce que los neurolépticos dieron lugar a una marcada reducción de la motilidad, en tanto que el clordiacepóxido y el fenobarbilal produjeron el efecto con· trario, probablemente como expresión de un efecto ansiolítico notable. La asociación de nialamida y 5-hidroxitriptofano dió lugar a una reducción muy significativa de la deambulación de las ratas tratadas, sugiriéndose una participación de la serotonina en los circuitos moduladores de la actividad de las ratas. Este descenso de movilidad fue hallado, asimtsmo, con la imipramina, r~lacio nándose también, este fenómeno, con una posible intensificación de sistemas serotoninérgicos. En cuanto a la intervención de los psicotropos sobre las estereotipias y las emisiones de bolos fecales, no se han encontrado resultados capaces de informar con eficacia, sobre la utilidad de estos dos parámetros como medida para la "emotividad"

Influência do genótipo sobre a produção e a composição do leite de cabras mestiças.

Resumo: O experimento foi conduzido na EMBRAPA Caprinos e objetivou determinar o potencial de produção e a composição do leite de cabras mestiças. Foram utilizadas 35 cabras, sendo doze ½ Alpina + ½ Moxotó (½ A-M), onze ¾ Alpina + ¼ Moxotó (¾ A-M) e doze "tricross" (½ Anglo-nubiana + ¼ Alpina + ½ Moxotó). O período de lactação foi de 147 dias. As cabras eram ordenhadas duas vezes ao dia e o controle leiteiro realizado semanalmente. Os animais receberam dieta para produção de 2,5 kg de leite/dia. A produção de leite, como coletada, não foi influenciada (P>0,05) pelo genótipo. No entanto, quando esta foi corrigida para 4% de gordura, o genótipo ½ A-M foi superior (P0,05). However, when milk production was adjusted for 4% of fat, the genotype ½ Alpina + ½ Moxotó was superior to the others (P<0,05). There was no difference among genotypes for milk fat, milk protein content and total milk solids

Nanoespumas de carbono: Producción por láser, propiedades químico-físicas y potenciales aplicaciones electroquímicas y en catálisis

Trabajo presentado a la "XII Reunion del Grupo Español del Carbón" celebrada en Madrid del 20 al 23 de octubre de 2013.Este trabajo ha sido financiado por la Comisión Europea (proyecto LIFE11/ENV/ES 560 “CERAMGLASS”) y el Gobierno de Aragón (Proyecto PI119/09).Peer Reviewe

Leftovers:The presence of manufacture-derived aquatic lipids in Alaskan Pottery

Lipids preserved within the walls of ancient pottery vessels are routinely analysed to reveal their original contents. The provenience of aquatic lipids in pottery is generally connected to vessel function (e.g., for cooking or storing fish, shellfish and aquatic mammals). However, ethnographic reports from early historic Alaska mention the use of aquatic oils for waterproofing low-fired pottery. Results of lipid residue studies on Alaskan pottery reflect an exclusive function of pottery to process aquatic resources. However, can one be sure these residues are the product of vessel function and not a remnant of the manufacturing process? The study presents the results of an experiment where the preservation of aquatic lipids during the firing process at different temperatures was measured. It was found that nearly all lipids were removed at firing temperatures of ≥ 400°C. Petrographic analysis of Alaskan pottery samples indicates that firing temperatures were generally &gt; 550°C but &lt; 800°C. The contribution of pre-firing manufacture-derived lipids to samples fired at these temperatures may be regarded as negligible. While the possible presence of aquatic lipids from post-firing surface treatments cannot be excluded, such treatments appear unnecessary for well-fired pottery.</p

Difusión del Corn Stunt Spiroplasma del maíz (Spiroplasma kunkelii) y del vector (Dalbulus maidis) en la República Argentina

Among the most important diseases that affect the corn crops (Zea mays L.) is “Corn Stunt Spiroplasm” (CSS). It is caused by Spiroplasm kunkelii and transmitted by a cicadelid Dalbulus maidis (De Long &amp; Wolcott, 1923), the only vector known up to the present in Argentina. Corn crop were monitored in 10 provinces of Argentina during 1991/1992, 1994/1995, 1996/2000. This provided information about the growth of this disease since its detection in the year 1990/1991 up to the present in Argentina as well as the distribution of D. maidis, its vector. The detection of the pathogen was performed on plants with symptoms that are characteristic of CSS using the technique of NC-ELISA. The vector was captured by sweeping plants of corn with symptoms. A increase in the areas affected by CSS was shown in the provinces of Jujuy, Salta, Tucumán, Catamarca, Chaco, Corrientes and Santiago del Estero as well as its recent detection in the provinces of Córdoba, San Luis and La Pampa. Based on the 83 places monitored, D. maidis was present in 80 % of the samples and the pathogen was detected in 96%. In the provinces Corrientes, Córdoba, San Luis and La Pampa, D. maidis was not registered.Entre las enfermedades más importantes que afectan al cultivo de maíz (Zea mays L.), se encuentra el achaparramiento o Corn Stunt Spiroplasma (CSS), causado por Spiroplasma kunkelii, trasmitido por el cicadélido Dalbulus maidis (De Long & Wolcott, 1923), único vector conocido hasta el presente en Argentina. Sobre la base de monitoreos realizados en lotes cultivados de maíz en 10 provincias de Argentina durante las campañas 1991/1992; 1994/1995; 1996/2000 se aporta información de esta enfermedad y del agente vector desde su detección en el año 1990/1991. La identificación del patógeno se realizó sobre plantas con síntomas característicos del CSS mediante la técnica de NCELISA. La presencia del vector se registró mediante capturas con red entomológica de arrastre sobre plantas de maíz con síntomas. Se demostró un incremento de la superficie afectada por el Corn Stunt Spiroplasma en las provincias de Jujuy, Salta, Tucumán, Catamarca, Chaco, Corrientes, Santiago del Estero y la reciente aparición en las provincias de Córdoba, San Luis y La Pampa. De las 83 localidades monitoreadas, D. maidis estuvo presente en 80 % de las localidades y en 96% se detectó el patógeno. En las provincias de Corrientes, Córdoba, San Luis y La Pampa no se registró a D. maidis

Geodesic motion in the space-time of a cosmic string

We study the geodesic equation in the space-time of an Abelian-Higgs string and discuss the motion of massless and massive test particles. The geodesics can be classified according to the particles energy, angular momentum and linear momentum along the string axis. We observe that bound orbits of massive particles are only possible if the Higgs boson mass is smaller than the gauge boson mass, while massless particles always move on escape orbits. Moreover, neither massive nor massless particles can ever reach the string axis for non-vanishing angular momentum. We also discuss the dependence of light deflection by a cosmic string as well as the perihelion shift of bound orbits of massive particles on the ratio between Higgs and gauge boson mass and the ratio between symmetry breaking scale and Planck mass, respectively.Comment: 20 pages including 14 figures; v2: references added, discussion on null geodesics extended, numerical results adde