Impact of outdoor air pollution on severity and mortality in COVID-19 pneumonia

The relationship between exposure to air pollution and the severity of coronavirus disease 2019 (COVID-19) pneumonia and other outcomes is poorly understood. Beyond age and comorbidity, risk factors for adverse outcomes including death have been poorly studied. The main objective of our study was to examine the relationship between exposure to outdoor air pollution and the risk of death in patients with COVID-19 pneumonia using individual-level data. The secondary objective was to investigate the impact of air pollutants on gas exchange and systemic inflammation in this disease. This cohort study included 1548 patients hospitalised for COVID-19 pneumonia between February and May 2020 in one of four hospitals. Local agencies supplied daily data on environmental air pollutants ($PM_{10}$, $PM_{2.5}$, $O_3$, $NO_2$, $NO$ and $NO_X$) and meteorological conditions (temperature and humidity) in the year before hospital admission (from January 2019 to December 2019). Daily exposure to pollution and meteorological conditions by individual postcode of residence was estimated using geospatial Bayesian generalised additive models. The influence of air pollution on pneumonia severity was studied using generalised additive models which included: age, sex, Charlson comorbidity index, hospital, average income, air temperature and humidity, and exposure to each pollutant. Additionally, generalised additive models were generated for exploring the effect of air pollution on C-reactive protein (CRP) level and Sp$O_2$/Fi$O_2$ at admission. According to our results, both risk of COVID-19 death and CRP level increased significantly with median exposure to $PM_{10}$, $NO_2$, $NO$ and $NO_X$, while higher exposure to $NO_2$, $NO$ and $NO_X$ was associated with lower Sp$O_2$/Fi$O_2$ ratios. In conclusion, after controlling for socioeconomic, demographic and health-related variables, we found evidence of a significant positive relationship between air pollution and mortality in patients hospitalised for COVID-19 pneumonia. Additionally, inflammation (CRP) and gas exchange (Sp$O_2$/Fi$O_2$) in these patients were significantly related to exposure to air pollution

The Effect of Volitional Preemptive Abdominal Contraction on Biomechanical Measures During A Front Versus Back Loaded Barbell Squat

# Background Weightlifting is growing in popularity among recreational and competitive athletes. The barbell back squat (BackS) is commonly included in these training programs, while the barbell front squat (FrontS) is commonly performed as a component of other lifts such as the power clean or clean and jerk, it is less commonly practiced in isolation. # Hypothesis/Purpose The purpose of this study was to examine the effects of VPAC performance on trunk muscle and LE biomechanical responses during loaded BackS versus FrontS in healthy subjects. # Study Design Controlled Laboratory Study # Methods Healthy male subjects with the ability to perform a sub-maximal loaded barbell squat lift were recruited. Subjects completed informed consent, demographic/medical history questionnaires and an instructional video. Subjects practiced VPAC and received feedback. Surface electromyography (sEMG) electrodes and kinematic markers were applied. Muscles included were the internal oblique (IO), external oblique (EO), rectus abdominis, iliocostalis lumborum (ICL), superficial multifidi, rectus femoris, vastus lateralis, biceps femoris, and gluteus maximus. Maximal voluntary isometric contractions established reference sEMG values. A squat one-rep-max (1RM) was predicted by researchers using a three to five repetition maximum (3RM, 5RM) load protocol. Subjects performed BackS trials at 75% 1RM while FrontS trials were performed at 75% BackS weight, both with and without VPAC. Subjects performed three repetitions of each condition with feet positioned on two adjacent force plates. Significant interactions and main effects were tested using a 2(VPAC strategy) x 2(squat variation) and 2(VPAC strategy) x 2(direction) within-subject repeated measures ANOVAs. Tukey's Post-Hoc tests identified the location of significant differences. # Results Trunk muscle activity was significantly higher during FrontS versus BackS regardless of VPAC condition. (IO: p=0.018, EO: p\<0.001, ICL: p\<0.001) VPAC increased performance time for both squat variations (p=.0011), which may be associated with decreased detrimental force potential on the lumbar spine and knees. VPAC led to improved ability to maintain a neutral lumbar spine during both squat variations. This finding is associated with decreased detrimental force potential on the lumbar spine. # Conclusions Findings could help guide practitioners and coaches to choose squat variations and incorporate VPAC strategies during their treatments and/or training programs. # Level of Evidence Level 3 ©The Author(s

Construction, assembly and tests of the ATLAS electromagnetic end-cap calorimeters

The construction and the assembly of the two end-caps of the ATLAS liquid argon electromagnetic calorimeter as well as their test and qualification programs are described. The work described here started at the beginning of 2001 and lasted for approximately three years. The results of the qualification tests performed before installation in the LHC ATLAS pit are given. The detectors are now installed in the ATLAS cavern, full of liquid argon and being commissioned. The complete detectors coverage is powered with high voltage and readout

Bacterial metabolism of algal extracellular carbon

Measurements of microbial utilization of extracellular organic carbon (EOC) released by phytoplankton commonly consider only EOC fractions subject to rapid uptake. Questions remain whether other EOC fractions are metabolized, what portion is labile, and with what assimilation efficiency this carbon substrate is utilized. 14 C-EOC was prepared by incubation of the natural mixed planktonic community from an oligotrophic lake with H 14 CO 3 in the light. 14 C-EOC which was not rapidly removed by heterotrophs remained in solution and was isolated by filtration. This residual EOC was inoculated with lake microheterotrophs in laboratory microcosms, and utilization kinetics were determined through long-term assays of cumulative 14 CO 2 production. Time-courses for 14 CO 2 production were consistent for all assays and were well described by a deterministic mixed-order degradation model. On twelve sampling occasions, from 29% to 76% of residual 14 C-EOC was labile to further metabolism by lake heterotrophs. First-order rate constants for EOC utilization showed a mode of 0.05 to 0.15 per day. From 33% to 78% of gross 14 C-EOC uptake was respired (mean 50%), indicating appreciable return of algal EOC to the pelagic food web as microbial biomass.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42876/1/10750_2004_Article_BF00015524.pd

Two Origins for the Gene Encoding α-Isopropylmalate Synthase in Fungi

BACKGROUND: The biosynthesis of leucine is a biochemical pathway common to prokaryotes, plants and fungi, but absent from humans and animals. The pathway is a proposed target for antimicrobial therapy. METHODOLOGY/PRINCIPAL FINDINGS: Here we identified the leuA gene encoding alpha-isopropylmalate synthase in the zygomycete fungus Phycomyces blakesleeanus using a genetic mapping approach with crosses between wild type and leucine auxotrophic strains. To confirm the function of the gene, Phycomyces leuA was used to complement the auxotrophic phenotype exhibited by mutation of the leu3+ gene of the ascomycete fungus Schizosaccharomyces pombe. Phylogenetic analysis revealed that the leuA gene in Phycomyces, other zygomycetes, and the chytrids is more closely related to homologs in plants and photosynthetic bacteria than ascomycetes or basidiomycetes, and suggests that the Dikarya have acquired the gene more recently. CONCLUSIONS/SIGNIFICANCE: The identification of leuA in Phycomyces adds to the growing body of evidence that some primary metabolic pathways or parts of them have arisen multiple times during the evolution of fungi, probably through horizontal gene transfer events

Ammonium regeneration: Its contribution to phytoplankton nitrogen requirements in a eutrophic environment

Ammonium regeneration, nutrient uptake, bacterial activity and primary production were measured from March to August 1980 in Bedford Basin, Nova Scotia, Canada, a eutrophic environment. Rates of regeneration and nutrient uptake were determined using 15N isotope dilution and tracer methodology. Although primary production, nutrient uptake and ammonium regeneration were significantly intercorrelated, no relationship was detected between these parameters and heterotrophic activity. The average contribution of ammonium to total nitrogen (ammonium+nitrate) uptake was similar in the spring and in the summer (approximately 60%). On a seasonal average basis, 36% of the phytoplankton ammonium uptake could be supplied by rapid remineralization processes. In spite of the high average contribution of NH4 regeneration to phytoplankton ammonia uptake, there is indirect evidence suggesting that other NH4 sources may occasionally be important

Fungal Planet description sheets: 868-950

Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetomella pseudocircinoseta and Coniella pseudodiospyri on Eucalyptus microcorys leaves, Cladophialophora eucalypti, Teratosphaeria dunnii and Vermiculariopsiella dunnii on Eucalyptus dunnii leaves, Cylindrium grande and Hypsotheca eucalyptorum on Eucalyptus grandis leaves, Elsinoe salignae on Eucalyptus saligna leaves, Marasmius lebeliae on litter of regenerating subtropical rainforest, Phialoseptomonium eucalypti (incl. Phialoseptomonium gen. nov.) on Eucalyptus grandis × camaldulensis leaves, Phlogicylindrium pawpawense on Eucalyptus tereticornis leaves, Phyllosticta longicauda as an endophyte from healthy Eustrephus latifolius leaves, Pseudosydowia eucalyptorum on Eucalyptus sp. leaves, Saitozyma wallum on Banksia aemula leaves, Teratosphaeria henryi on Corymbia henryi leaves. Brazil, Aspergillus bezerrae, Backusella azygospora, Mariannaea terricola and Talaromyces pernambucoensis from soil, Calonectria matogrossensis on Eucalyptus urophylla leaves, Calvatia brasiliensis on soil, Carcinomyces nordestinensis on Bromelia antiacantha leaves, Dendryphiella stromaticola on small branches of an unidentified plant, Nigrospora brasiliensis on Nopalea cochenillifera leaves, Penicillium alagoense as a leaf endophyte on a Miconia sp., Podosordaria nigrobrunnea on dung, Spegazzinia bromeliacearum as a leaf endophyte on Tilandsia catimbauensis, Xylobolus brasiliensis on decaying wood. Bulgaria, Kazachstania molopis from the gut of the beetle Molops piceus. Croatia, Mollisia endocrystallina from a fallen decorticated Picea abies tree trunk. Ecuador, Hygrocybe rodomaculata on soil. Hungary, Alfoldia vorosii (incl.Alfoldia gen. nov.) from Juniperus communis roots, Kiskunsagia ubrizsyi (incl. Kiskunsagia gen. nov.) from Fumana procumbens roots. India, Aureobasidium tremulum as laboratory contaminant, Leucosporidium himalayensis and Naganishia indica from windblown dust on glaciers. Italy, Neodevriesia cycadicola on Cycas sp. leaves, Pseudocercospora pseudomyrticola on Myrtus communis leaves, Ramularia pistaciae on Pistacia lentiscus leaves, Neognomoniopsis quercina (incl. Neognomoniopsis gen. nov.) on Quercus ilex leaves. Japan, Diaporthe fructicola on Passiflora edulis × P. edulis f. flavicarpa fruit, Entoloma nipponicum on leaf litter in a mixed Cryptomeria japonica and Acer spp. forest. Macedonia, Astraeus macedonicus on soil. Malaysia, Fusicladium eucalyptigenum on Eucalyptus sp. twigs, Neoacrodontiella eucalypti (incl. Neoacrodontiella gen. nov.) on Eucalyptus urophylla leaves. Mozambique, Meliola gorongosensis on dead Philenoptera violacea leaflets. Nepal, Coniochaeta dendrobiicola from Dendriobium lognicornu roots. New Zealand, Neodevriesia sexualis and Thozetella neonivea on Archontophoenix cunninghamiana leaves. Norway, Calophoma sandfjordenica from a piece of board on a rocky shoreline, Clavaria parvispora on soil, Didymella finnmarkica from a piece of Pinus sylvestris driftwood. Poland, Sugiyamaella trypani from soil. Portugal, Colletotrichum feijoicola from Acca sellowiana. Russia, Crepidotus tobolensis on Populus tremula debris, Entoloma ekaterinae, Entoloma erhardii and Suillus gastroflavus on soil, Nakazawaea ambrosiae from the galleries of Ips typographus under the bark of Picea abies. Slovenia, Pluteus ludwigii on twigs of broadleaved trees. South Africa, Anungitiomyces stellenboschiensis (incl. Anungitiomyces gen. nov.) and Niesslia stellenboschiana on Eucalyptus sp. leaves, Beltraniella pseudoportoricensis on Podocarpus falcatus leaf litter, Corynespora encephalarti on Encephalartos sp. leaves, Cytospora pavettae on Pavetta revoluta leaves, Helminthosporium erythrinicola on Erythrina humeana leaves, Helminthosporium syzygii on a Syzygium sp. barkcanker, Libertasomyces aloeticus on Aloe sp. leaves, Penicillium lunae from Musa sp. fruit, Phyllosticta lauridiae on Lauridia tetragona leaves, Pseudotruncatella bolusanthi (incl. Pseudotruncatellaceae fam. nov.) and Dactylella bolusanthi on Bolusanthus speciosus leaves. Spain, Apenidiella foetida on submerged plant debris, Inocybe grammatoides on Quercus ilex subsp. ilex forest humus, Ossicaulis salomii on soil, Phialemonium guarroi from soil. Thailand, Pantospora chromolaenae on Chromolaena odorata leaves. Ukraine, Cadophora helianthi from Helianthus annuus stems. USA, Boletus pseudopinophilus on soil under slash pine, Botryotrichum foricae, Penicillium americanum and Penicillium minnesotense from air. Vietnam, Lycoperdon vietnamense on soil. Morphological and culture characteristics are supported by DNA barcodes

Impacto cuantitativo de la contaminación en la probabilidad de muerte por neumonía por SARS-CoV-2

Introducción La evidencia científica disponible señala que la contaminación del aire exterior podría agravar la severidad de la COVID-19 y por ende, incrementar las probabilidades de fallecimiento. Material y métodos Estudio observacional longitudinal retrospectivo de cohortes, multicéntrico en 4 hospitales: 2 en Bizkaia (1 urbano, 1 urbano-rural), Valencia y Barcelona (urbanos). Se incluyeron ingresos por neumonía SARS-CoV-2 en el primer pico epidémico de COVID-19 (febrero-mayo 2020). Para determinar la exposición a contaminación por PM$_{10}$ y NO$_{2}$, se obtuvieron los datos publicados por los organismos autonómicos de calidad del aire, para 2019 y 1er semestre 2020. Se utilizó un Modelo Aditivo Generalizado (GAM) para estimar el nivel diario de contaminante en cada código postal, en función de las coordenadas geográficas y la altitud de las estaciones de medición [Figura 1]. Para determinar la exposición crónica, se calcularon media y máximo en 2019; la aguda se caracterizó por media y máximo en los 7 días anteriores al ingreso. Se estudió la razón de probabilidades (‘odds ratio’, OR) de muerte frente a supervivencia entre nuestra cohorte. Se modeló mediante un GAM con regresión logística, incorporando como efectos fijos sexo, edad y contaminante; hospital como efecto aleatorio e índice de comorbilidad de Charlson como función suave mediantes splines penalizados. Resultados De los 1548 pacientes reclutados, 243 (15.7%) fallecieron durante su hospitalización y/o 30 días postingreso. Según los modelos [Tabla 1], existe evidencia estadística significativa de que la exposición crónica a PM$_{10}$ y NO$_{2}$ incrementan la probabilidad de muerte por neumonía SARS-CoV-2. Compensando por sexo, edad y Charlson -todos factores relacionados positivamente con el OR de muerte- así como por hospital; por cada incremento de 10 μg/m$^{3}$ en el nivel de PM$_{10}$ (máximo anual) el OR aumenta en 10.5%, linealmente proporcional al incremento en la contaminación. Mientras, cada 10 μg/m$^{3}$ más de NO2 (media anual) aumentan OR en 35.7%; cada 10 μg/m$^{3}$ más en exposición aguda a NO2 (media semana pre-ingreso): 62.9%; y NO$_{2}$ (máximo semana): 34.4%. Conclusiones Se cuantificaron y compensaron los efectos de los factores sexo, edad, Charlson y hospital. A igualdad de estos, incrementos en la exposición crónica y aguda a PM$_{10}$ y NO$_{2}$ aumentan de manera lineal y estadísticamente significativa la probabilidad de muerte por neumonía SARS-CoV-2