Microbial community structure mediates response of soil C decomposition to litter addition and warming

Microbial activity has been highlighted as one of the main unknowns controlling the fate and turnover of soil organic matter (SOM) in response to climate change. How microbial community structure and function may (or may not) interact with increasing temperature to impact the fate and turnover of SOM, in particular when combined with changes in litter chemistry, is not well understood. The primary aim of this study was to determine if litter chemistry impacted the decomposition of soil and litter-derived carbon (C), and its interaction with temperature, and whether this response was controlled by microbial community structure and function. Fresh or pre-incubated eucalyptus leaf litter (13C enriched) was added to a woodland soil and incubated at 12, 22, or 32 �C. We tracked the movement of litter and soilderived C into CO2, water-extractable organic carbon (WEOC), and microbial phospholipids (PLFA). The litter additions produced significant changes in every parameter measured, while temperature, interacting with litter chemistry, predominately affected soil C respiration (priming and temperature sensitivity), microbial community structure, and the metabolic quotient (a proxy for microbial carbon use efficiency [CUE]). The direction of priming varied with the litter additions (negative with fresh litter, positive with pre-incubated litter) and was related to differences in the composition of microbial communities degrading soil-C, particularly gram-positive and gram-negative bacteria, resulting from litter addition. Soil-C decomposition in both litter treatments was more temperature sensitive (higher Q10) than in the soil-only control, and soil-C priming became increasingly positive with temperature. However, microbes utilizing soil-C in the litter treatments had higher CUE, suggesting the longer-term stability of soil-C may be increased at higher temperature with litter addition. Our results show that in the same soil, the growth of distinct microbial communities can alter the turnover and fate of SOM and, in the context of global change, its response to temperature

Carbon isotope discrimination by C3 pasture grasses along a rainfall gradient in South Australia: implications for palaeoecological studies

Canberr

Soil organic matter decomposition and turnover in a tropical Ultisol: evidence from δ¹³C, δ¹⁵N and geochemistry

Soil organic matter (SOM), leaf litter, and root material of an Ultisol from the tropical rainforest of Kakamega, Kenya, were analyzed for stable carbon (delta-13C) and nitrogen (delta-15N) isotopic values as well as total organic carbon (TOC) and total nitrogen (TN) contents in order to determine trends in SOM decomposition within a very well-developed soil under tropical conditions. In addition, we quantified mineralogy and chemistry of the inorganic soil fraction. Clay mineralogical variation with depth was small and the abundance of kaolin indicates intense weathering and pedoturbation under humid tropical conditions. The soil chemistry was dominated by silica, aluminium, and iron with calcium, potassium, and magnesium as minor constituents. The relative depletion of base cations compared with silica and aluminium is an indicator for intense weathering and leaching conditions over long periods of time. Depth profiles of delta-13C and delta-15N showed a distinct enrichment trend down profile with a large (average 13Delta-C = 5.0 per mil average 15Delta-N = 6.3 per mil) and abrupt offset within the uppermost 10-20 cm of the soil. Isotopic enrichment with depth is commonly observed in soil profiles and has been attributed to fractionation during decomposition. However, isotopic offsets within soil profiles that exceed 3 per mil are usually interpreted as a recent change from C4 to C3 dominated vegetation. We argue that the observed isotopic depth profiles along with data from mineralogy and chemistry of the inorganic fraction from the Kakamega Forest soil are a result of intense weathering and high organic matter turnover rates under humid tropical conditions.The Radiocarbon archives are made available by Radiocarbon and the University of Arizona Libraries. Contact [email protected] for further information.Migrated from OJS platform February 202

The influence of feedstock and production temperature on biochar carbon chemistry: a solid-state 13C NMR study

Solid-state 13C nuclear magnetic resonance (NMR) spectroscopy was used to evaluate the carbon chemistry of twenty-six biochars produced from eleven different feedstocks at production temperatures ranging from 350°C to 600°C. Carbon-13 NMR spectra were acquired using both cross-polarisation (CP) and direct polarisation (DP) techniques. Overall, the corresponding CP and DP spectra were similar, although aromaticity was slightly higher and observability much higher when DP was used. The relative size and purity of the aromatic ring structures (i.e. aromatic condensation) were also gauged using the ring current technique. Both aromaticity and aromatic condensation increased with increasing production temperature, regardless of the feedstock source. However, there were clear differences in these two measures for biochars produced at the same temperature but from different feedstocks. Based on a relationship previously established in a long-term incubation study between aromatic condensation and the mean residence time (MRT) of biochar, the MRT of the biochars was estimated to range from <260 years to >1400 years. This study demonstrates how the combination of feedstock composition and production temperature influences the composition of aromatic domains in biochars, which in turn is likely to be related to their recalcitrance and ultimately their carbon sequestration value

The influence of feedstock and production temperature on biochar carbon chemistry: a solid-state 13C NMR study

Solid-state 13C nuclear magnetic resonance (NMR) spectroscopy was used to evaluate the carbon chemistry of twenty-six biochars produced from eleven different feedstocks at production temperatures ranging from 350°C to 600°C. Carbon-13 NMR spectra were acquired using both cross-polarisation (CP) and direct polarisation (DP) techniques. Overall, the corresponding CP and DP spectra were similar, although aromaticity was slightly higher and observability much higher when DP was used. The relative size and purity of the aromatic ring structures (i.e. aromatic condensation) were also gauged using the ring current technique. Both aromaticity and aromatic condensation increased with increasing production temperature, regardless of the feedstock source. However, there were clear differences in these two measures for biochars produced at the same temperature but from different feedstocks. Based on a relationship previously established in a long-term incubation study between aromatic condensation and the mean residence time (MRT) of biochar, the MRT of the biochars was estimated to range from <260 years to >1400 years. This study demonstrates how the combination of feedstock composition and production temperature influences the composition of aromatic domains in biochars, which in turn is likely to be related to their recalcitrance and ultimately their carbon sequestration value

Palaeoenvironmental mosaic of Proconsul habitats: geochemical and sedimentalogical interpretation of Kisingiri fossil sites, Western Kenya

THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD, ENGLAND, OX5 1G

Geochemistry (delta13C, delta15N, 13C NMR) and residence times (14C and OSL) of soil organic matter from red-brown earths of South Australia: implications for soil genesis

Soil forming processes important to the development of Red-Brown Earths (duplex soils) in southeastern Australia have been investigated by a combination of techniques, including isotopic (δ13C, δ15N, 14C), spectroscopic (13C NMR, MIR), optically stimulated luminescence dating (OSL) and phytolith analyses. A distinct increase in clay content, corresponding changes in the abundance of major elements, as well as changes in organic chemistry (13C NMR), stable isotope trends (δ13C, δ15N), and phytolith abundance, are apparent in the transition from the very sandy A horizon to the clayey B horizon in three soil profiles from the Coonawarra–Padthaway region of South Australia. These structural and chemical changes between the A and the B horizons are associated with an abrupt increase in both 14C (bulk soil organic matter) and OSL burial ages of individual quartz grains. While previous interpretations have promoted the formation of duplex red-brown earths as due to clay illuviation, we propose a two-stage soil formation, which may be related to paleoclimatic changes during and after the Last Glacial Maximum. Our data suggest that a major part of the A horizon was aeolian derived and was deposited over the last 10,000 years, whereas much of the B horizon, although originally aeolian, has been extensively modified over much longer periods of time (tens of thousands of years). These results indicate the influence of different substrates (sandy versus clayey), process and time for formation as well as paleoclimatic history on the physical properties of the soil and the chemical characteristics of the organic matter within the soil profile