Rehabilitation of severely resorbed maxillae with zygomatic implants : an update

Studies highlight the zygomatic bone as a suitable anatomical structure for implant placements since they cross four corticals. Zygomatic implants were described by Branemark in 1998, since then zygomatic implants are indicated in maxillae with atrophy of the posterior area. They have been used in systemic diseases associated with bone loss in this area, and in patients who have suffered radical surgery for maxillofacial tumors. Computed tomography is recommended before placement in order to discount any pathology of the maxillary sinus. The surgical technique has been slightly modified since its description with procedures such as the sinus slot technique. The success rate obtained by different authors varies between 82% and 100%, indicating this technique as a valid treatment option. The objective of this study was to revise the literature with the aim of updating the subject

Analysis and adsorption-interaction of amino acids on basaltic mineral subjected to different simulated atmospheres

[EN] The research have been conducted in order to have a better understanding of amino acid adsorption on volcanic matrix surface subjected to different extreme environmental conditions (Martian surface environment, space environment, UV-environment, etc.) simulated by The Planetary Atmosphere and Surfaces Chamber (PASC) at the ”INTA-Centro de Astrobiogia”. The spectroscopic measurements were done by Raman and IR spectroscopy (at the Unidad Asociada Uva-CSIC at Centro de Astrobiología) taking into account the capabilities of the combined Raman-IR analyses for astrobiological target such as the detection of biomarkers on the future ESA-ExoMars Mission. Moreover, SEM-EDX analyses have been conducted in order to complement the spectroscopic results, which endeavour the use of spectroscopic systems for space missions. Conclusion: The L-aspartic acid shows to be the most suitable amino acid for this kind of experiment; the amino acid half–life is 104 seconds according to the reference; both spectroscopic techniques, working in synergy, have detected the amino acid degradation, but amino acid mineral interaction was not detected; the experiments have shown the versatility of the simulation chamber (PASC) to perform experiments under different planetary environments.[ES] Los experimentos se han realizado con el objectivo de tener un mejor entendimiento sobre aminoácidos inmovilizados sobre matrices de caracter volcánico y estos han sido sometidos a diferentes ambientes extremos (Ambiente marciano, ambiente espacial, ambiente de radiación UV, etc.) dentro de la Cámara de Simulación de atmosferas y superficies planetarias (PASC) en el Centro de Astrobiología- INTA. Los métodos espectroscópicos usados son la espectroscopía Raman e Infrarroja (en la Unidad Asociada Uva-CSIC al Centro de Astrobiología) teniendo en cuenta las capacidades combinadas de estos sistemas para objetivos astrobiológicos como la detección de biomarcadores en la futura misión ESAExoMars. Además, se realizaron medidas mediante microscopía electrónica (SEM-EDX) las cuales complementan los resultados espectroscópicos y refuerzan el uso de estos sistemas para misiones espaciales. Conclusiones: El ácido L-Aspartico demostró ser el más eficiente para este tipo de experimentos; la vida media de los aminoácido es de 104 teniendo en cuenta las referencias; ambos sistemas espectroscópicos Raman-IR, trabajando conjuntamente, detectaron la degradación de los aminoácidos, pero la interacción entre aminoácido-mineral no fue detectada; los experimentos demostraron la versatilidad de la Cámara de Simulación (PASC) en cuanto a la realizacion de experimentos en distintos ambientes planetarios.Peer reviewe

Spectroelectrochemical operando method for monitoring a phenothiazine electrografting process on amide functionalized C-nanodots/Au hybrid electrodes

Phenothiazine derivatives are extensively explored dye molecules, which present interesting electrochemical and optical properties. In recent years, the possibility of transforming some phenothiazines in their aryl diazonium salt derivatives has been proved, what allows them to be electrochemically reduced and electrografted onto conductive surfaces. This is a smart way to modify these surfaces and enable them with specific functionalities. In order to better comprehend the electrografting process and consequently have a higher control of it, in this work we have carried out an exhaustive study by operando UV–Vis spectroelectrochemistry of the electrografting of a phenothiazine aryl diazonium salt onto amide carbon nanodots. As a model of phenothiazine dye we have chosen Azure A. The electrografting onto carbon nanodots has been stablished by comparison with the results obtained on bare gold electrodes in this novel study. The presence of carbon dots improves the reversibility of the electrochemical process as derived from the results obtained by operando UV–Vis spectroelectrochemistry. In addition, to asses that the electrochemical process studied corresponds to the electrografting, the results have been compared to those obtained for the simple Azure A adsorption. This study shows the advantages of obtaining simultaneously the electrochemical and the spectroscopic evolution of an electron-transfer process in a single experiment, in a particular electrochemical reaction. This work could be the starting point for the study of the electrografting on other nanomaterialsFunding from the Spanish Ministerio de Ciencia, Innovación y Universidades (project: CTQ2017-84309-C2-1-R) and Comunidad Autónoma de Madrid (NANOAVANSENS Program) is acknowledged. IMDEA Nanociencia acknowledges support from the 'Severo Ochoa' Programme for Centres of Excellence in R&D (Ministerio de Ciencia, Innovación y Universidades, Grant SEV-2016-0686

Strip-based lateral flow-type indicator displacement assay for gamma-hydroxybutyric acid (GHB) detection in beverages

[EN] The use of gamma-hydroxybutyric acid (GHB) in drug-facilitated sexual assault has increased due to its availability and high solubility in aqueous solutions and alcoholic beverages, necessitating the development of rapid methods for GHB detection. In this respect, portable testing methods for use in the field, based on lateral flow assays (LFAs) and capable of detecting trace concentrations of target analytes, are particularly attractive and hold enormous potential for the detection of illicit drugs. Using this strategy, here we report a rapid, low cost, easy-tohandle strip-based LFA for GHB analysis employing a smartphone for fluorescence readout. At molecular signalling level, the ensemble is based on a Cu2+ complex with a tetradentate ligand and the fluorescent dye coumarin 343, which indicate GHB through an indicator displacement assay (IDA) in aqueous solution. When incorporated in a LFA-based strip test this system shows a detection limit as low as 0.03 mu M for GHB in MES buffer solution and is able to detect GHB at concentrations of 0.1 mu M in soft drinks and alcoholic beverages in only 1 min.This research was funded by the Ministerio de Ciencia, Innovacion y Universidades (Spanish Government), the Agencia Estatal de Investigacion (AEI), the European Union (PID2021-126304OB-C41) (MCIU/AEI/FEDER, EU), the Conselleria de Innovacion, Universidades, Ciencia y Sociedad Digital, Generalitat Valenciana (PROMETEO CIPROM/2021/007) and the German Research Foundation (DFG; CL 761/1-19). This work was also supported by CIBER-Consorcio Centro de Investigacion Biomedica en Red (CB06/01/2012), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovacion. E. G. is grateful to the Spanish MEC for her FPU grant (FPU16/02464). G. H. thanks to Generalitat Valenciana for his pre-doctoral grant (ACIF/2021/204). V. M.-C. thanks the financial support from Generalitat Valenciana (CIDEGENT/2020/031).Garrido-García, EM.; Hernández-Sigüenza, G.; Climent Terol, E.; Marcos Martínez, MD.; Rurack, K.; Gaviña, P.; Parra, M.... (2023). Strip-based lateral flow-type indicator displacement assay for gamma-hydroxybutyric acid (GHB) detection in beverages. Sensors and Actuators B Chemical. 377. https://doi.org/10.1016/j.snb.2022.13304337

Carbon nanodots modified-electrode for peroxide-free cholesterol biosensing and biofuel cell design

The determination of cholesterol is greatly important because high concentrations of this biomarker are associated to heart disease. Moreover, cholesterol can be used as a fuel in enzymatic fuel cells operating under physiological conditions. Here, we present a cholesterol biosensor and a peroxide-free biofuel cell based on the electrocatalytic oxidation of the NADH generated during the enzymatic reaction of cholesterol dehydrogenase (ChDH) as an alternative to the H2O2 biosensing strategies used with cholesterol oxidase-bioelectrodes. Azure A functionalized-carbon nanodots were used as NADH oxidation electrocatalysts and for ChDH covalent immobilization. The biosensor responded linearly to cholesterol concentrations up to 1.7 mM with good sensitivity (4.50 mA cm−2 M−1) and at a low potential. The ChDH bioelectrode was combined with an O2-reducing bilirubin oxidase cathode to produce electrical energy using cholesterol as fuel and O2 as oxidant. Furthermore, the resulting enzymatic fuel cell was tested in human serum naturally containing free cholesterolA.L.DL. and M.P. thank MCIU/AEI/FEDER, EU for funding project RTI2018–095090-B-I00. M.B. acknowledges funding from the European Union’s Horizon 2020 Research and Innovation Program under the Marie Skłodowska-Curie grant agreement No. 713366. This work was also supported by Talent Attraction Project from CAM (SI3/PJI/ 2021–00341 and 2021–5A/BIO-20943), Spanish Ministerio de Ciencia e Innovacion (PID2020–116728RB-I00) and TRANSNANOAVANSENSCAM Program (S2018/NMT-4349

Azure A embedded in carbon dots as NADH electrocatalyst: Development of a glutamate electrochemical biosensor

Carbon nanodots modified with azure A (AA-CDs) have been synthesized and applied as redox mediator of bioelectrocatalytic reactions. A deep characterization of AA-CDs nanomaterial has been carried out, proving the covalent attachment of azure A molecules into the carbon dots nanostructure. Disposable screen-printed carbon electrodes (SPCE) have been modified with AA-CDs, through the action of chitosan polymer (Chit-AA-CDs/SPCE). The Chit-AA-CDs/SPCE electrocatalytic activity towards the oxidation of NADH has been proved, obtaining excellent results regarding the low oxidation potential achieved (−0.15 V vs. Ag) and low detection and quantification limits (LOD and LOQ) for NADH, 16 and 53 µM, respectively. The developed electrochemical platform has been applied for the construction of a glutamate biosensor by immobilizing L-glutamic dehydrogenase (GLDH/Chit-AA-CDs/SPCE). The morphology of GLDH/Chit-AA-CDs/SPCE platform was analysed by AFM at each different step of the electrode modification process. The resulting biosensing platform is capable of detect NADH enzymatically generated by GLDH in the presence of glutamate and NAD+. Good analytical parameters were obtained for glutamate analysis using GLDH/Chit-AA-CDs/SPCE, as LOD and LOQ of 3.3 and 11 µM, respectively. The biosensor has been successfully applied to the analysis of food and biological samplesThis work has been supported by the Spanish Ministerio de Ciencia e Innovacion (PID2020–116728RB-I00) and Comunidad Autonoma de Madrid (SI3/PJI/2021–00341, P2018/NMT-4349 TRANSNANOAVANSENS Program

The impact of ABCDE bundle implementation on patient outcomes: a nationwide cohort study

Background: The ABCDE bundle is a set of evidence-based practices to systematically reduce the risks of sedation, delirium, and immobility in intensive care patients. Implementing the bundle improves clinical outcome. Aims and Objectives: To investigate the association between patient outcomes and compliance with bundle components ABC (analgosedation algorithms), D (delirium protocol), and E (early mobilization protocol). Design: A Spanish multicentre cohort study of adult patients receiving invasive mechanical ventilation (IMV) for ≥48 h until extubation. Methods: The primary outcome was pain level, cooperation to permit Medical Research Council Scale administration, patient days of delirium, and mobility. The secondary outcome was cumulative drug dosing by IMV days. Tertiary outcomes (ICU days, IMV days, bed rest days, ICU mortality, ICUAW) and independent variables (analgosedation, delirium, early mobilization protocols) were also studied. Results: Data were collected from 605 patients in 80 ICUs and 5214 patient days with IMV. Two-thirds of the ICUs studied applied no protocols. Pain was not assessed on 83.6% of patient days. Patient cooperation made scale administration feasible on 20.7% of days. Delirium and immobility were found on 4.2% and 69.9% of days, respectively. Patients had shorter stays in ICUs with bundle protocols and fewer days of IMV in ICUs with delirium and mobilization bundle components (P = 0.006 and P = 0.03, respectively). Analgosedation protocols were associated with more opioid dosing (P = 0.02), and delirium and early mobilization protocols with more propofol (P = 0.001), dexmedetomidine (P = 0.001), and lower benzodiazepine dosing (P = 0.008). Conclusions: The implementation rate of ABCDE bundle components was very low in our Spanish setting, but when implemented, patients had a shorter ICU stay, more analgesia dosing, and lighter sedation. Relevance to clinical practice: Applying some but not all the bundle components, there is increased analgesia and light sedation drug use, decreased benzodiazepines, and increased patient cooperation and mobility, resulting in a shorter ICU stay and fewer days of IMV

Feasibility of the internet attachment-based compassion therapy in the general population: protocol for an open-label uncontrolled pilot trial

Background: Compassion-based interventions delivered over the internet are showing promising results for the promotion of psychological health and well-being. Several studies have highlighted their feasibility, acceptance, and preliminary efficacy. However, this is an incipient field of research, and to the best of our knowledge, there are no data available from Spanish-speaking countries. Objective: The aim of this study is to investigate the feasibility, acceptance, and preliminary efficacy of the Internet Attachment-Based Compassion Therapy (iABCT), a web-based version of the Attachment-Based Compassion Therapy, in Spanish speakers from the general population. Methods: This feasibility study features a single-arm, uncontrolled, within-group design with an embedded qualitative and quantitative process evaluation at baseline, immediately after the intervention and at the 3-month follow-up. A minimum of 35 participants from the general population will be allocated to iABCT. Feasibility measures will include attrition rate, patterns of use of the web-based system, and participants' acceptability, usability, and opinion. The primary outcome was measured using the Pemberton Happiness Index. Secondary outcomes were measured using the Compassion Scale, Self-Compassion Scale, Forms of Self-Criticizing/Attacking and Self-Reassuring Scale-Short form, Five Facets of Mindfulness Questionnaire, Relationships Questionnaire, General Health Questionnaire, Non-Attachment Scale, International Positive and Negative Affect Schedule Short Form, Purpose-In-Life Test, and difficulties regarding the practice of compassion (Compassion Practice Quality Questionnaire). Mixed models will be used to evaluate primary and secondary outcome measures. A qualitative content analysis of the participants' qualitative responses will also be performed. Results: Enrollment started in February 2020 and will be finished in April 2020. Data analysis will start in October 2020. Conclusions: To our knowledge, this study will, for the first time, show data on the feasibility, acceptability, and preliminary efficacy of web-based compassion (and self-compassion) training-that is, the adapted iABCT-in Spanish speakers from the general population. Further aspects of their implementation (ie, facilitators, barriers, and unwanted effects) and mechanisms of change will be investigated. This study will allow the revision and fine-tuning of the developed intervention, study design, and planning procedures, as well as the initiation of a future randomized controlled trial

A hotspot for posttranslational modifications on the androgen receptor dimer interface drives pathology and anti-androgen resistance

Mutations of the androgen receptor (AR) associated with prostate cancer and androgen insensitivity syndrome may profoundly influence its structure, protein interaction network, and binding to chromatin, resulting in altered transcription signatures and drug responses. Current structural information fails to explain the effect of pathological mutations on AR structure-function relationship. Here, we have thoroughly studied the effects of selected mutations that span the complete dimer interface of AR ligand-binding domain (AR-LBD) using x-ray crystallography in combination with in vitro, in silico, and cell-based assays. We show that these variants alter AR-dependent transcription and responses to anti-androgens by inducing a previously undescribed allosteric switch in the AR-LBD that increases exposure of a major methylation target, Arg761. We also corroborate the relevance of residues Arg761 and Tyr764 for AR dimerization and function. Together, our results reveal allosteric coupling of AR dimerization and posttranslational modifications as a disease mechanism with implications for precision medicine

The impact of surgical practice on oncological outcomes in robot-assisted radical hysterectomy for early-stage cervical cancer, Spanish National Registry

Minimal invasive surgery (MIS) has been associated with lower disease-free survival than open surgery among women who underwent radical hysterectomy for early-stage cervical cancer. However, the mechanisms by which MIS increases mortality in cervical cancer remain uncertain. We aimed to determine if surgical practice among centers using robotic surgery has an impact on oncological outcomes. We evaluated 215 women with early-stage cervical cancer (≤IB1 or IIA1, FIGO 2009) who underwent robot-assisted radical hysterectomy in five Spanish tertiary centers between 2009 and 2018. A higher surgical volume, higher participation in clinical trials, higher rate of MRI use for diagnosis, greater use of sentinel lymph node biopsies, and a favorable learning curve with low rates of early recurrences were observed for the centers with better oncological outcomes. These factors might have a significant impact on oncological outcomes in all surgical approaches. Abstract: This study aimed to assess whether surgical practice had a significant impact on oncological outcomes among women who underwent robot-assisted radical hysterectomy for early-stage cervical cancer (≤IB1 or IIA1, FIGO 2009). The secondary objective was to audit the pre-surgical quality indicators (QI) proposed by the European Society of Gynaecological Oncology (ESGO). The top 5 of 10 centers in Spain and Portugal were included in the analysis. The hospitals were divided into group A (n = 118) and group B (n = 97), with recurrence rates of 10%, respectively. After balancing both groups using the propensity score, the ORs for all events were higher and statistically significant for group B (recurrences OR = 1.23, 95% CI = 1.13-1.15, p-value = 0.001; death OR = 1.10, 95% CI = 1.02-1.18, p-value = 0.012; disease-specific mortality ORr = 1.11, 95% CI = 1.04-1.19, p-value = 0.002). A higher surgical volume, higher participation in clinical trials, higher rate of MRI use for diagnosis, greater use of sentinel lymph node biopsies, and a favorable learning curve with low rates of early recurrences were observed among the centers with better oncological outcomes. These factors might have a significant impact on oncological outcomes not only after robot-assisted surgery, but also after laparoscopies and open surgeries in the treatment of cervical cancer