Protección de los trabajadores frente al riesgo de exposición a radiaciones ionizantes en la industria de fosfatos en Portugal

230 p.En este trabajo de investigación, se pretende desarrollar y aplicar un método para la evaluación de riesgos para la salud de los trabajadores debidos a la exposición ocupacional a radiaciones ionizantes en las industrias de fertilizantes que utilizan fosforita como materia prima. Basado en la literatura disponible se identifican los efectos negativos para la salud de los trabajadores expuestos a radiaciones ionizantes, de acuerdo con los tipos y niveles de exposición a que están sometidos y se caracterizan las radiaciones ionizantes desde un punto de vista físico, energético y de capacidad de penetración. Además se analizan los métodos y los equipos de medición más adecuados para los trabajos de campo a realizar en el caso del estudio presentad

The application of supercritical fluids technology to recover healthy valuable compounds from marine and agricultural food processing by-products: a review

Food by-products contain a remarkable source of bioactive molecules with many benefits for humans; therefore, their exploitation can be an excellent opportunity for the food sector. Moreover, the revalorization of these by-products to produce value-added compounds is considered pivotal for sustainable growth based on a circular economy. Traditional extraction technologies have several drawbacks mainly related to the consumption of hazardous organic solvents, and the high temperatures maintained for long extraction periods which cause the degradation of thermolabile compounds as well as a low extraction efficiency of desired compounds. In this context, supercritical fluid extraction (SFE) has been explored as a suitable green technology for the recovery of a broad range of bioactive compounds from different types of agri-food wastes. This review describes the working principle and development of SFE technology to valorize by-products from different origin (marine, fruit, vegetable, nuts, and other plants). In addition, the potential effects of the extracted active substances on human health were also approached.Axencia Galega de Innovación | Ref. IN607A2019 / 01European Commission | Ref. H2020, AQUABIOPRO-FIT, n. 790956CYTED | Ref. 119RT0568Ministerio de Economía y Competitividad | Ref. RYC2018-026177-IChina Scholarship Council (CSC) | Ref. 201908420246China Scholarship Council (CSC) | Ref. 201908420245Generalitat Valenciana | Ref. IDIFEDER / 2018 / 04

Plant-growth promotion by proteobacterial strains depends on the availability of phosphorus and iron in Arabidopsis thaliana plants

Phosphorus (as phosphate, Pi) and iron (Fe) are critical nutrients in plants that are often poorly available in the soil and can be microbially affected. This work aimed to evaluate how plant-rhizobacteria interaction changes due to different Pi or Fe nutritional scenarios and to study the underlying molecular mechanisms of the microbial modulation of these nutrients in plants. Thus, three proteobacteria (Paraburkholderia phytofirmans PsJN, Azospirillum brasilense Sp7, and Pseudomonas putida KT2440) were used to inoculate Arabidopsis seeds. Additionally, the seeds were exposed to a nutritional factor with the following levels for each nutrient: sufficient (control) or low concentrations of a highly soluble source or sufficient concentrations of a low solubility source. Then, the effects of the combinatorial factors were assessed in plant growth, nutrition, and genetic regulation. Interestingly, some bacterial effects in plants depended on the nutrient source (e.g., increased aerial zones induced by the strains), and others (e.g., decreased primary roots induced by Sp7 or KT2440) occurred regardless of the nutritional treatment. In the short-term, PsJN had detrimental effects on plant growth in the presence of the low-solubility Fe compound, but this was not observed in later stages of plant development. A thorough regulation of the phosphorus content was detected in plants independent of the nutritional treatment. Nevertheless, inoculation with KT2440 increased P content by 29% Pi-deficiency exposed plants. Conversely, the inoculation tended to decrease the Fe content in plants, suggesting a competition for this nutrient in the rhizosphere. The P-source also affected the effects of the PsJN strain in a double mutant of the phosphate starvation response (PSR). Furthermore, depending on the nutrient source, PsJN and Sp7 strains differentially regulated PSR and IAA- associated genes, indicating a role of these pathways in the observed differential phenotypical responses. In the case of iron, PsJN and SP7 regulated iron uptake-related genes regardless of the iron source, which may explain the lower Fe content in inoculated plants. Overall, the plant responses to these proteobacteria were not only influenced by the nutrient concentrations but also by their availabilities, the elapsed time of the interaction, and the specific identities of the beneficial bacteria.Graphical AbstractThe effects of the different nutritional and inoculation treatments are indicated for plant growth parameters (A), gene regulation (B) and phosphorus and iron content (C). Figures created with BioRender.com with an academic license

A DEM approach for simulating flexible beam elements with the Project Chrono core module in DualSPHysics

This work presents a novel approach for simulating elastic beam elements in DualSPHysics leveraging functions made available by the coupling with the Project Chrono library. Such numerical frameworks, belonging to the Meshfree Particle Methods family, stand out for several features, like complex multiphase phenomena, moving boundaries, and high deformations which are handled with relative ease and reasonable numerical stability and reliability. Based on a co-rotating rigid element structure and lumped elasticity, a cogent mathematical formulation, relying on the Euler–Bernoulli beam theory for the structural discretization, is presented and applied to simulating two-dimensional flexible beams with the discrete elements method (DEM) formulation. Three test cases are presented to validate the smoothed particle hydrodynamics-based (SPH) structure model in both accuracy and stability, starting from an equilibrium test, to the dynamic response, and closing with a fluid–structure interaction simulation. This work proves that the developed theory can be used within a Lagrangian framework, using the features provided by a DEM solver, overtaking the initial limitations, and hence applying the results of static theories to complex dynamic problems.Xunta de Galicia | Ref. ED431C 2021/44Xunta de Galicia | Ref. ED481A-2021/337Ministerio de Ciencia, Innovación y Universidades | Ref. IJCI-2017-32592Agencia Estatal de Investigación | Ref. PID2020-113245RB-I0

Regular wave seakeeping analysis of a planing hull by smoothed particle hydrodynamics: a comprehensive validation

In this work, the dynamics of a planing hull in regular head waves was investigated using the Smoothed Particle Hydrodynamics (SPH) meshfree method. The simulation of the interaction of such vessels with wave trains features several challenging characteristics, from the complex physical interaction, due to large dynamic responses, to the likewise heavy numerical workload. A novel numerical wave flume implemented within the SPH-based code DualSPHysics fulfills both demands, guaranteeing comparable accuracy with an established proprietary Computational Fluid Dynamics (CFD) solver without sharpening the computational load. The numerical wave flume uses ad hoc open-boundary conditions to reproduce the flow characteristics encountered by the hull during its motion, combining the current and waves while adjusting their properties with respect to the vessel’s experimental towing speed. It follows a relatively small three-dimensional domain, where the potentiality of the SPH method in modeling free-surface flows interacting with moving structures is unleashed. The results in different wave conditions show the feasibility of this novel approach, considering the overall good agreement with the experiments; hence, an interesting alternative procedure to simulate the seakeeping test in several marine conditions with bearable effort and satisfying accuracy is established.Ministerio de Ciencia e Innovación | Ref. PID2020-113245RBI00Xunta de Galicia | Ref. ED431C 2021/44Ministerio de Ciencia e Innovación | Ref. TED2021-129479AI00Xunta de Galicia | Ref. ED481A-2021/337Ministerio de Ciencia e Innovación | Ref. RYC2020-030197-

Development and characterization of a novel sustainable probiotic goat whey cheese containing second cheese whey powder and stabilized with thyme essential oil and sodium citrate

Probiotic goat whey cheeses with added second cheese whey powder (SCWP) were developed, resulting in creamy and spreadable products. The products contained Lactobacillus rhamnosus and Bifidobacterium animalis, as well as thyme essential oil and sodium citrate. Matrices of probiotic whey cheeses, with and without additives, were produced and stored at 5 °C for 21 days. Microbial and chemical profiles were evaluated weekly. The composition of the optimum matrix, formulated with whey cheese, probiotic culture, SCWP, thyme essential oil and sodium citrate (WCPSTC) was, expressed in % (w/w): protein (10.78 ± 0.08), fat (7.59 ± 0.03), dry matter (25.64 ± 0.13), ash (2.81 ± 0.02) and lactose (3.16 ± 0.04). Viable cell numbers of both probiotic cultures in matrix WCPSTC remained above 107 CFU g−1. This finding is of the utmost importance since it proves that both probiotic bacteria, citrate and thyme essential oil can be combined in order to increase the shelf-life and functional value of dairy products. All matrices’ pH values decreased during storage, yet only matrix WCPSTC remained above 5.0 pH units. The results indicated that the development of a probiotic whey cheese incorporating a dairy by-product, SCWP, is possible without compromising its chemical, microbiological or sensorial stability.info:eu-repo/semantics/publishedVersio

The recombinant cysteine proteinase B (CPB) from Leishmania braziliensis and its domains: promising antigens for serodiagnosis of cutaneous and visceral leishmaniasis in dogs.

Leishmaniasis represents a group of parasitic diseases caused by a protozoan of the genus Leishmania and is widely distributed in tropical and subtropical regions. Leishmaniasis is one of the major tropical neglected diseases, with 1.5 to 2 million new cases occurring annually. Diagnosis remains a challenge despite advances in parasitological, serological, and molecular methods. Dogs are an important host for the parasite and develop both visceral and cutaneous lesions. Our goal was to contribute to the diagnosis of canine cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) using the recombinant cysteine proteinase B (F-CPB) from Leishmania braziliensis and its N- and C-terminal domains (N-CPB and C-CPB) as antigens in an enzyme-linked immunosorbent assay (ELISA). Sera from dogs from Northwest Argentina diagnosed with CL were tested by ELISA against a supernatant of L. braziliensis lysate, the F-CPB protein, and its domains. We found values of sensitivity (Se) of 90.7%, 94.4%, and 94.3% and specificity (Sp) of 95.5%, 90.9%, and 91.3% for F-CPB and its N- and C-terminal domains, respectively. In sera from dogs diagnosed with VL from Northeast Argentina, we found Se of 93.3%, 73.3%, and 66.7% and Sp of 92.3%, 76.9%, and 88.5% for F-CPB and its N- and C-terminal domains, respectively. These results support CPB as a relevant antigen for canine leishmaniasis diagnosis in its different clinical presentations. More interestingly, the amino acid sequence of CPB showed high percentages of identity in several Leishmania species, suggesting that the CPB from L. braziliensis qualifies as a good antigen for the diagnosis of leishmaniasis caused by different species.Fil: Bivona, Augusto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Czentner Colomo, Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Sanchez Alberti, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Cerny, Natacha. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Cardoso Landaburu, Alejandro Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Nevot, María Cecilia. Veterinaria del Oeste; ArgentinaFil: Estevez, José Octavio. Veterinaria del Oeste; ArgentinaFil: Marco, Jorge Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Basombrío, Manuel Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Patología Experimental. Universidad Nacional de Salta. Facultad de Ciencias de la Salud. Instituto de Patología Experimental; ArgentinaFil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; ArgentinaFil: Cazorla, Silvia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones en Microbiología y Parasitología Médica. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentin

Coupling an SPH-based solver with an FEA structural solver to simulate free surface flows interacting with flexible structures

This work proposes a two-way coupling between a Smoothed Particle Hydrodynamics (SPH) model-based named DualSPHysics and a Finite Element Analysis (FEA) method to solve fluid–structure interaction (FSI). Aiming at having a computationally efficient solution via spatial adjustable resolutions for the two phases, the SPH-FEA coupling herein presented implements the Euler–Bernoulli beam model, based on a simplified model that incorporates axial and flexural deformations, to introduce a solid solver in the DualSPHysics framework. This approach is particularly functional and very precise for slender beam elements undergoing large displacements, and large deformations can also be experienced by the structural elements due to the non-linear FEA implementation via a co-rotational formulation. In this two-way coupling, the structure is discretised in the SPH domain using boundary particles on which the forces exerted by fluid phases are computed. Such forces are passed over to the FEA structural solver that updates the beam shape and, finally, the particle positions are subsequently reshuffled to represent the deformed shape at each time step. The SPH-FEA coupling is validated against four reference cases, which prove the model to be as accurate as other approaches presented in literature.Ministerio de Ciencia e Innovación | Ref. PID2020-113245RB-I00Ministerio de Ciencia e Innovación | Ref. TED2021-129479A-I00Xunta de Galicia | Ref. ED431C 2021/44Xunta de Galicia | Ref. ED481A-2021/337Universidade de Vigo/CISU

Anticoagulant Activity of a Unique Sulfated Pyranosic (133) - ß - L - Arabinan through Direct Interaction with Thrombin

Fernández, Paula Virginia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Química de Biomoléculas. Buenos Aires, Argentina.Quintana, Irene. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Laboratorio de Hemostasia y Trombosis. Buenos Aires, Argentina.Cerezo, Alberto S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Subsede del Centro de Investigación de Hidratos de Carbono (CIHIDECAR). Ciudad Universitaria, Buenos Aires, Argentina.Caramelo, Julio J. CONICET - Instituto de Investigaciones Bioquímicas de Buenos Aires (IIBBA). Buenos Aires, Argentina.Pol Fachin, Laercio. Universidade Federal do Rio Grande do Sul. Faculdade de Farmácia. Programa de Pos-Graduação em Biologia Celular e Molecular. Centro de Biotecnologia. Rio Grande do Sul, Brasil.Verli, Hugo. Universidade Federal do Rio Grande do Sul.Centro de Biotecnologia. Programa de Pos-Graduação em Biologia Celular e Molecular. Rio Grande do Sul, Brasil.Estevez, José Manuel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIByNE). Buenos Aires, Argentina.Ciancia, Marina. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Química de Biomoléculas. Buenos Aires, Argentina.223–233Background: Many seaweed polysaccharides have anticoagulant activity, but the mechanism of action was elucidated in a few cases. Results: A highly sulfated pyranosic ß-arabinan exerts its activity through direct and indirect inhibition of thrombin. Conclusion: The structure and mechanism of action of the arabinan are different from those found for other polysaccharides. Significance: This arabinan could be an alternative anticoagulant in certain specific cases

Coupling of an SPH-based solver with a multiphysics library

Financiado para publicación en acceso aberto: Universidade de Vigo/CISUGA two-way coupling between the Smoothed Particle Hydrodynamics-based (SPH) code with a multiphysics library to solve complex fluid-solid interaction problems is proposed. This work provides full access to the package for the use of this coupling by releasing the source code, completed with guidelines for its compilation and utilization, and self-contained template setups for practical uses of the novel implemented features, is provided here. The presented coupling expands the applicability of two different solvers allowing to simulate fluids, multibody systems, collisions with frictional contacts using either non-smooth contact (NSC) or smooth contact (SMC) methods, all integrated under the same framework. The fluid solver is the open-source code DualSPHysics, highly optimised for simulating free-surface phenomena and structure interactions, uniquely positioned as a general-purpose Computational Fluid Dynamics (CFD) software with a GPU-accelerated solver. Mechanical systems that comprise collision detection and/or multibody dynamics are solved by the multiphysics library Project Chrono, which uses a Discrete Element Method (DEM). Therefore, this SPH-DEM coupling approach can manage interactions between fluid and complex multibody systems with relative constraints, springs, or mechanical joints.Ministerio de Ciencia e Innovación | Ref. PID2020-113245RB-I00Xunta de Galicia | Ref. ED431C 2021/44Xunta de Galicia | Ref. ED481A-2021/337Ministerio de Ciencia e Innovación, Xunta de Galicia con fondos de la Unión Europea NextGenerationEU y el Fondo Europeo Marítimo y de Pesca | Ref. PRTR-C17.I