25 research outputs found
Optimizing RNA Library Preparation to Redefine the Translational Status of 80S Monosomes: A Dissertation
Deep sequencing of strand-specific cDNA libraries is now a ubiquitous tool for identifying and quantifying RNAs in diverse sample types. The accuracy of conclusions drawn from these analyses depends on precise and quantitative conversion of the RNA sample into a DNA library suitable for sequencing. Here, we describe an optimized method of preparing strand-specific RNA deep sequencing libraries from small RNAs and variably sized RNA fragments obtained from ribonucleoprotein particle footprinting experiments or fragmentation of long RNAs. Because all enzymatic reactions were optimized and driven to apparent completion, sequence diversity and species abundance in the input sample are well preserved. This optimized method was used in an adapted ribosome-profiling approach to sequence mRNA footprints protected either by 80S monosomes or polysomes in S. cerevisiae. Contrary to popular belief, we show that 80S monosomes are translationally active as demonstrated by strong three-nucleotide phasing of monosome footprints across open reading frames. Most mRNAs exhibit some degree of monosome occupancy, with monosomes predominating on upstream ORFs, canonical ORFs shorter than ~590 nucleotides and any ORF for which the total time required to complete elongation is substantially shorter than the time required for initiation. Additionally, endogenous NMD targets tend to be monosome-enriched. Thus, rather than being inactive, 80S monosomes are significant contributors to overall cellular translation
An optimized kit-free method for making strand-specific deep sequencing libraries from RNA fragments
Deep sequencing of strand-specific cDNA libraries is now a ubiquitous tool for identifying and quantifying RNAs in diverse sample types. The accuracy of conclusions drawn from these analyses depends on precise and quantitative conversion of the RNA sample into a DNA library suitable for sequencing. Here, we describe an optimized method of preparing strand-specific RNA deep sequencing libraries from small RNAs and variably sized RNA fragments obtained from ribonucleoprotein particle footprinting experiments or fragmentation of long RNAs. Our approach works across a wide range of input amounts (400 pg to 200 ng), is easy to follow and produces a library in 2-3 days at relatively low reagent cost, all while giving the user complete control over every step. Because all enzymatic reactions were optimized and driven to apparent completion, sequence diversity and species abundance in the input sample are well preserved
Processing of joint molecule intermediates by structure-selective endonucleases during homologous recombination in eukaryotes
Homologous recombination is required for maintaining genomic integrity by functioning in high-fidelity repair of DNA double-strand breaks and other complex lesions, replication fork support, and meiotic chromosome segregation. Joint DNA molecules are key intermediates in recombination and their differential processing determines whether the genetic outcome is a crossover or non-crossover event. The Holliday model of recombination highlights the resolution of four-way DNA joint molecules, termed Holliday junctions, and the bacterial Holliday junction resolvase RuvC set the paradigm for the mechanism of crossover formation. In eukaryotes, much effort has been invested in identifying the eukaryotic equivalent of bacterial RuvC, leading to the discovery of a number of DNA endonucleases, including Mus81–Mms4/EME1, Slx1–Slx4/BTBD12/MUS312, XPF–ERCC1, and Yen1/GEN1. These nucleases exert different selectivity for various DNA joint molecules, including Holliday junctions. Their mutant phenotypes and distinct species-specific characteristics expose a surprisingly complex system of joint molecule processing. In an attempt to reconcile the biochemical and genetic data, we propose that nicked junctions constitute important in vivo recombination intermediates whose processing determines the efficiency and outcome (crossover/non-crossover) of homologous recombination
Redefining the Translational Status of 80S Monosomes
Fully assembled ribosomes exist in two populations: polysomes and monosomes. While the former has been studied extensively, to what extent translation occurs on monosomes and its importance for overall translational output remain controversial. Here, we used ribosome profiling to examine the translational status of 80S monosomes in Saccharomyces cerevisiae. We found that the vast majority of 80S monosomes are elongating, not initiating. Further, most mRNAs exhibit some degree of monosome occupancy, with monosomes predominating on nonsense-mediated decay (NMD) targets, upstream open reading frames (uORFs), canonical ORFs shorter than approximately 590 nt, and ORFs for which the total time required to complete elongation is substantially shorter than that required for initiation. Importantly, mRNAs encoding low-abundance regulatory proteins tend to be enriched in the monosome fraction. Our data highlight the importance of monosomes for the translation of highly regulated mRNAs
TMPRSS2-ERG fusions linked to prostate cancer racial health disparities : a focus on Africa
BACKGROUND : The androgen‐regulated gene TMPRSS2 to the ETS transcription factor
gene ERG fusion is the most common genomic alteration acquired during prostate
tumorigenesis and biased toward men of European ancestry. In contrast, African
American men present with more advanced disease, yet their tumors are less likely to
acquire TMPRSS2‐ERG. Data for Africa is scarce.
METHODS : RNA was made available for genomic analyses from 181 prostate tissue
biopsy cores from Black South African men, 94 with and 87 without pathological
evidence for prostate cancer. Reverse transcription polymerase chain reaction was
used to screen for the TMPRSS2‐ERG fusion, while transcript junction coordinates and
isoform frequencies, including novel gene fusions, were determined using targeted
RNA sequencing.
RESULTS : Here we report a frequency of 13% for TMPRSS2‐ERG in tumors from Black
South Africans. Present in 12/94 positive versus 1/87 cancer negative prostate tissue
cores, this suggests a 92.62% predictivity for a positive cancer diagnosis (P = 0.0031).
At a frequency of almost half that reported for African Americans and roughly a
quarter of that reported for men of European ancestry, acquisition of TMPRSS2‐ERG
appears to be inversely associated with aggressive prostate cancer. Further support
was provided by linking the presence of TMPRSS2‐ERG to low‐grade disease in
younger patients (P = 0.0466), with higher expressing distal ERG fusion junction coordinates.
CONCLUSIONS : Only the second study of its kind for the African continent, we support a
link between TMPRSS2‐ERG status and prostate cancer racial health disparity beyond
the borders of the United States. We call for urgent evaluation of androgen
deprivation therapy within Africa.The
Cancer Association of South Africa (CANSA) and National Research
Foundation (NRF) South Africa for funds to support and maintain the
SAPCS (to MSRB. and VMH). The project was further supported by a
University of Sydney Bridging Support Grant to V.M.H. (#G199756),
with additional support from the Australian Prostate Cancer Research
Centre (APCRC) NSW. National Health and Medical Research Council
(NHMRC) grants support JB (#APP1108254 and APP1114016) and
EEH (#APP1103685), while VMH is supported by the University of
Sydney Foundation and Petre Foundation, Australia.http://wileyonlinelibrary.com/journal/prosam2020School of Health Systems and Public Health (SHSPH