85 research outputs found
Induction of annexin-1 during TRAIL-induced apoptosis in thyroid carcinoma cells
We investigated the expression of annexin-1 (ANXA1) in thyroid carcinoma cell lines and in thyroid cancers with a different degree of differentiation. The highest level of ANXA1 expression examined by Western blotting was detected in the papillary carcinoma cells (NPA) and in the follicular cells (WRO). On the other hand, the most undifferentiated thyroid carcinoma cells (ARO and FRO) presented the lowest level of ANXA1 expression. In surgical tissue specimens from 32 patients with thyroid cancers, we found high immunoreactivity for ANXA1 in papillary (PTC) and follicular (FTC) thyroid cancers while in undifferentiated thyroid cancers (UTC) the expression of the protein was barely detectable. Control thyroid tissue resulted positive for ANXA1. In summary, 70% of UTC examined weakly expressed ANXA1, whereas 65% of PTC or FTC specimens tested showed high expression of the protein. Thus ANXA1 expression may correlate with the tumorigenesis suggesting that the protein may represent an effective differentiation marker in thyroid cancer
Augmented Reality to Guide Selective Clamping and Tumor Dissection During Robot-assisted Partial Nephrectomy: A Preliminary Experience.
ABSTRACT Introduction to explore the feasibility of augmented reality (AR) to guide arterial clamping during robot-assisted partial nephrectomy (RAPN). Patients and Methods 15 consecutive patients with T1 renal mass underwent RAPN guided by AR. The 3D virtual model derived by computed tomography was superimposed on the actual view provided by the Da Vinci video stream thought AR technology. Preoperative plan of arterial clamping based on 2D conventional imaging, on 3D model and the effective intraoperative surgical approach guided by AR were compared using the McNeamar test. Results The plan of arterial clamping based on 2D preoperative imaging was recorded as follows: no clamping in 3 (20%), clamping of the main artery in 10 (66.7%) and selective clamping in 1 (6.7%) and super-selective clamping in 1 (6.7%) cases. After revision of the 3D model, the plan of clamping was modified as follows: no clamping in 1 (6.7%), clamping of the main artery in 2 (13.3%), selective clamping in 8 (53.3%) and super-selective clamping in 4 (26.7%) cases (p=0.03). The effective intraoperative clamping approach guided by AR-guidance was performed as planned in 13 (86.7%) patients. Conclusion AR for 3D guided renal surgery is useful to increase the adoption of selective clamping during RAPN
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Circulating Cancer Stem Cell-Derived Extracellular Vesicles as a Novel Biomarker for Clinical Outcome Evaluation.
The recent introduction of the "precision medicine" concept in oncology pushed cancer research to focus on dynamic measurable biomarkers able to predict responses to novel anticancer therapies in order to improve clinical outcomes. Recently, the involvement of extracellular vesicles (EVs) in cancer pathophysiology has been described, and given their release from all cell types under specific stimuli, EVs have also been proposed as potential biomarkers in cancer. Among the techniques used to study EVs, flow cytometry has a high clinical potential. Here, we have applied a recently developed and simplified flow cytometry method for circulating EV enumeration, subtyping, and isolation from a large cohort of metastatic and locally advanced nonhaematological cancer patients (Nâ=â106); samples from gender- and age-matched healthy volunteers were also analysed. A large spectrum of cancer-related markers was used to analyse differences in terms of peripheral blood circulating EV phenotypes between patients and healthy volunteers, as well as their correlation to clinical outcomes. Finally, EVs from patients and controls were isolated by fluorescence-activated cell sorting, and their protein cargoes were analysed by proteomics. Results demonstrated that EV counts were significantly higher in cancer patients than in healthy volunteers, as previously reported. More interestingly, results also demonstrated that cancer patients presented higher concentrations of circulating CD31+ endothelial-derived and tumour cancer stem cell-derived CD133â+âCD326- EVs, when compared to healthy volunteers. Furthermore, higher levels of CD133â+âCD326- EVs showed a significant correlation with a poor overall survival. Additionally, proteomics analysis of EV cargoes demonstrated disparities in terms of protein content and function between circulating EVs in cancer patients and healthy controls. Overall, our data strongly suggest that blood circulating cancer stem cell-derived EVs may have a role as a diagnostic and prognostic biomarker in cancer
Corrigendum to âCirculating Cancer Stem Cell-Derived Extracellular Vesicles as a Novel Biomarker for Clinical Outcome Evaluationâ
[This corrects the article DOI: 10.1155/2019/5879616.].Peer Reviewe
A multicenter epidemiological study on second malignancy in non-syndromic pheochromocytoma/paraganglioma patients in Italy
SIMPLE SUMMARY: As no previous studies had assessed the risk of second malignant tumors in patients with pheochromocytomas/paragangliomas (PPGLs), we aimed to evaluate whether these patients could have an increased risk of additional malignancy, comparing them with patients in the general population who had a first malignancy and developed a second malignant tumor. We demonstrated that PPGL patients had higher incidence of additional malignant tumors and the risk of developing a second malignant tumor increased with age at diagnosis. As the main tumors were prostate, colorectal and lung/bronchial cancers in males, and breast cancer, differentiated thyroid cancer and melanoma in females, our findings could have an impact on the surveillance strategy. ABSTRACT: No studies have carried out an extensive analysis of the possible association between non-syndromic pheochromocytomas and paragangliomas (PPGLs) and other malignancies. To assess >the risk of additional malignancy in PPGL, we retrospectively evaluated 741 patients with PPGLs followed-up in twelve referral centers in Italy. Incidence of second malignant tumors was compared between this cohort and Italian patients with two subsequent malignancies. Among our patients, 95 (12.8%) developed a second malignant tumor, which were mainly prostate, colorectal and lung/bronchial cancers in males, breast cancer, differentiated thyroid cancer and melanoma in females. The standardized incidence ratio was 9.59 (95% CI 5.46â15.71) in males and 13.21 (95% CI 7.52â21.63) in females. At multivariable analysis, the risk of developing a second malignant tumor increased with age at diagnosis (HR 2.50, 95% CI 1.15â5.44, p = 0.021 for 50â59 vs. 60- vs. <50-year). In patients with available genetic evaluation, a positive genetic test was inversely associated with the risk of developing a second tumor (HR 0.25, 95% CI 0.10â0.63, p = 0.003). In conclusion, PPGLs patients have higher incidence of additional malignant tumors compared to the general population who had a first malignancy, which could have an impact on the surveillance strategy
TOI-1235 b: A Keystone Super-Earth For Testing Radius Valley Emergence Models Around Early M Dwarfs
Small planets on close-in orbits tend to exhibit envelope mass fractions of either effectively zero or up to a few percent depending on their size and orbital period. Models of thermally driven atmospheric mass loss and of terrestrial planet formation in a gas-poor environment make distinct predictions regarding the location of this rocky/nonrocky transition in periodâradius space. Here we present the confirmation of TOI-1235 b (P = 3.44 days, ), a planet whose size and period are intermediate between the competing model predictions, thus making the system an important test case for emergence models of the rocky/nonrocky transition around early M dwarfs (R s = 0.630 ± 0.015 , M s = 0.640 ± 0.016 ). We confirm the TESS planet discovery using reconnaissance spectroscopy, ground-based photometry, high-resolution imaging, and a set of 38 precise radial velocities (RVs) from HARPS-N and HIRES. We measure a planet mass of , which implies an iron core mass fraction of % in the absence of a gaseous envelope. The bulk composition of TOI-1235 b is therefore consistent with being Earth-like, and we constrain an H/He envelope mass fraction to be \u3c0.5% at 90% confidence. Our results are consistent with model predictions from thermally driven atmospheric mass loss but not with gas-poor formation, suggesting that the former class of processes remains efficient at sculpting close-in planets around early M dwarfs. Our RV analysis also reveals a strong periodicity close to the first harmonic of the photometrically determined stellar rotation period that we treat as stellar activity, despite other lines of evidence favoring a planetary origin ( days, ) that cannot be firmly ruled out by our data
Toi-1235 b: A keystone super-earth for testing radius valley emergence models around early m dwarfs
Small planets on close-in orbits tend to exhibit envelope mass fractions of
either effectively zero or up to a few percent depending on their size and
orbital period. Models of thermally-driven atmospheric mass loss and of
terrestrial planet formation in a gas-poor environment make distinct
predictions regarding the location of this rocky/non-rocky transition in
period-radius space. Here we present the confirmation of TOI-1235 b (
days, R), a planet whose size and
period are intermediate between the competing model predictions, thus making
the system an important test case for emergence models of the rocky/non-rocky
transition around early M dwarfs ( R,
M). We confirm the TESS planet discovery using
reconnaissance spectroscopy, ground-based photometry, high-resolution imaging,
and a set of 38 precise radial-velocities from HARPS-N and HIRES. We measure a
planet mass of M which implies an iron core
mass fraction of % in the absence of a gaseous envelope. The
bulk composition of TOI-1235 b is therefore consistent with being Earth-like
and we constrain a H/He envelope mass fraction to be % at 90% confidence.
Our results are consistent with model predictions from thermally-driven
atmospheric mass loss but not with gas-poor formation, which suggests that the
former class of processes remain efficient at sculpting close-in planets around
early M dwarfs. Our RV analysis also reveals a strong periodicity close to the
first harmonic of the photometrically-determined stellar rotation period that
we treat as stellar activity, despite other lines of evidence favoring a
planetary origin ( days,
M) that cannot be firmly ruled out by our data
An ultrahot Neptune in the Neptune desert
About one out of 200 Sun-like stars has a planet with an orbital period
shorter than one day: an ultra-short-period planet (Sanchis-ojeda et al. 2014;
Winn et al. 2018). All of the previously known ultra-short-period planets are
either hot Jupiters, with sizes above 10 Earth radii (Re), or apparently rocky
planets smaller than 2 Re. Such lack of planets of intermediate size (the "hot
Neptune desert") has been interpreted as the inability of low-mass planets to
retain any hydrogen/helium (H/He) envelope in the face of strong stellar
irradiation. Here, we report the discovery of an ultra-short-period planet with
a radius of 4.6 Re and a mass of 29 Me, firmly in the hot Neptune desert. Data
from the Transiting Exoplanet Survey Satellite (Ricker et al. 2015) revealed
transits of the bright Sun-like star \starname\, every 0.79 days. The planet's
mean density is similar to that of Neptune, and according to thermal evolution
models, it has a H/He-rich envelope constituting 9.0^(+2.7)_(-2.9)% of the
total mass. With an equilibrium temperature around 2000 K, it is unclear how
this "ultra-hot Neptune" managed to retain such an envelope. Follow-up
observations of the planet's atmosphere to better understand its origin and
physical nature will be facilitated by the star's brightness (Vmag=9.8)
An ultrahot Neptune in the Neptune desert
About 1 out of 200 Sun-like stars has a planet with an orbital period shorter than one day: an ultrashort-period planet. All of the previously known ultrashort-period planets are either hot Jupiters, with sizes above 10 Earth radii (Râ), or apparently rocky planets smaller than 2âRâ. Such lack of planets of intermediate size (the âhot Neptune desertâ) has been interpreted as the inability of low-mass planets to retain any hydrogen/helium (H/He) envelope in the face of strong stellar irradiation. Here we report the discovery of an ultrashort-period planet with a radius of 4.6âRâ and a mass of 29âMâ, firmly in the hot Neptune desert. Data from the Transiting Exoplanet Survey Satellite revealed transits of the bright Sun-like star LTT 9779 every 0.79âdays. The planetâs mean density is similar to that of Neptune, and according to thermal evolution models, it has a H/He-rich envelope constituting 9.0^(+2.7)_(â2.9)% of the total mass. With an equilibrium temperature around 2,000âK, it is unclear how this âultrahot Neptuneâ managed to retain such an envelope. Follow-up observations of the planetâs atmosphere to better understand its origin and physical nature will be facilitated by the starâs brightness (V_(mag)â=â9.8)
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