Losses of expression of the antigens A, Lea and Lex and over-expression of Ley in carcinomas and HG-SIL of the uterine cervix

<p>Abstract</p> <p>Background</p> <p>The glycosylation of a great number of molecules, glyco-protein or glycolipids, has been of interest for decades.</p> <p>Objective</p> <p>To compare the expressive patterns of the isoantigenic determinants of histo-blood groups ABH and Lewis in squamous and simple epithelium and in precursors and cancers of the cervix.</p> <p>Methods</p> <p>A total of 36 lesions and neoplasms (10 LG-SIL, 16 HG-SIL and 10 invasive carcinomas) have been studied with immunohistochemical techniques, using monoclonal antibodies (MoAb BG1 to BG8) for precursor chains, blood-group ABH and Lewis group Le^a, Le^b, Le^x, and Le^y, and four types of lectins. In addition, we have studied the expression of p53 protein and PCNA, establishing the rate of proliferation of each lesion. Using PCR techniques, we have also detected part of the intron of the E6 gene of HPV-16.</p> <p>Results</p> <p>In the invasive cervical carcinomas, we observed a loss of expression of the Le^xantigen (p < 0.01). With regard to the progression of the different lesions studied, we found alterations in the patterns of expression of the antigens of the ABH and Lewis blood groups. There was a tendency towards a loss of expression and heterogeneous patterns in the more advanced lesions, as well as over-expression of the Le^yantigens. With PCNA, we established a proliferative rate which tended to be greater in relation to the progression of the cervix neoplasms.</p> <p>Conclusion</p> <p>These results indicate that there is a relation between the losses of histo-blood groups and the progression of the squamous intraepithelial lesions.</p

Deaf bilingual education: key tenets for strategic change

Pese a los progresos logrados en la protección de los derechos fundamentales de salud y educación de las personas sordas usuarias de las lenguas de signos, a menudo se sigue negando a niños sordos su derecho a aprender y usar las lenguas de signos en su educación y en entornos físicos y psicosociales accesibles de conformidad con la Convención de los Derechos de las Personas con Discapacidad (CDPD) y con las recientes leyes 27/2007 y 17/2007. El objetivo principal de este estudio fue obtener información sobre los factores estratégicos que favorecen una escuela saludable e inclusiva, mediante programas de educación bilingüe sostenibles y de calidad para escolares sordos en España. La metodología utilizada consistió en un estudio descriptivo con componentes de investigación-acción participativa y un enfoque de análisis estratégico. Como resultados, cabe destacar que las principales macrotendencias que, en el ámbito internacional, han promovido el cambio hacia prácticas bilingües son dos: en primer lugar, los cambios sociales y políticos que tienden a una creciente aceptación de la diversidad y los temas relacionados con las personas sordas; y en segundo lugar, una tendencia a un activismo, una autoconciencia y un apoderamiento crecientes por parte de las personas sordas. Como contrapartida, las que lo han obstaculizado son, por un lado, la visión de la sordera como una condición médica, que puede solucionarse mediante la tecnología; y por otro lado, el fonocentrismo, unido a una cierta resistencia social a lo desconocido. Aunque en España algunas de las escuelas que han iniciado el cambio cumplen las recomendaciones de la CDPD, la mayoría están todavía en una fase preliminar de formulación o puesta en práctica de este nuevo modelo y distan mucho de encontrarse distribuidas de manera igualitaria en las diferentes comunidades autónomas. Como conclusión podemos decir que conseguir una educación más saludable e inclusiva para los escolares sordos implica poner en marcha una estrategia de actuación planificada en la que participen activamente todos los sectores y organismos relevantes implicados y, en particular, las comunidades sordas a través de las organizaciones que las representan. Sin un cambio radical de perspectiva, la salud y la educación no serán accesibles a todos y las desigualdades sociales se verán acentuadas.Although progress has been made in protecting deaf sign language users’ fundamental rights to health and education, all too often deaf children are still denied the right to learn and use sign languages in their education and in accessible physical and psycho-social environments in accordance with both the Convention on the Rights of Persons with Disabilities (CRPD) and recent Spanish laws (Act 27/2007 and Act 17/2007). The main objective of the present study was to collect information about some strategic factors that may facilitate healthy, inclusive schooling through sustainable, high-quality deaf bilingual programmes in Spain. A descriptive study was conducted, with some components of participatory action research and a strategic analysis framework. The two main megatrends found to have spurred the current change toward bilingual education in order of priority are: (1) societal and political changes towards a growing acceptance of diversity and Deaf issues and (2) growing Deaf activism, selfawareness and empowerment. The two main hindering megatrends are: (1) the view of deafness as a medical condition with a technological solution and (2) phonocentrism and societal resistance to the unknown. Although some schools have achieved CRPD-recommended standards, the majority of deaf bilingual programmes in Spain are still in a very early stage of formulation and implementation and are far from being equally distributed across the country. Transforming schools into healthier, more inclusive settings for deaf children implies the implementation of a well-planned strategy involving the active participation of relevant sectors and organizations, particularly deaf communities through their representative organizations. Unless there is a radical change of perspective, health and education will not be available to all, and therefore social inequalities will increase.Fondo de Investigación Sanitaria del Ministerio de Sanidad y Consumo; Vicerrectorado de Investigación, Desarrollo e Innovación; Vicerrectorado de Alumnado; y Grupo de Investigación en Salud Pública de la Universidad de Alicante

Proceso para la obtención y purificación de B-Ficoeritrina

Número de publicación: ES2197820 A1 (01.01.2004) También publicado como: ES2197820 B1 (01.03.2005) Número de Solicitud: Consulta de Expedientes OEPM (C.E.O.) P200201398 (17.06.2002)Proceso en tres etapas para la obtención y purificación de la proteína B-ficoeritrina procedente de la microalga Porphyridium cruentum caracterizado por su alto rendimiento. La primera etapa consiste en una ruptura celular encaminada a liberar el material citoplasmático mediante un proceso de choque osmótico usando un tapón de ácido acético/acetato sódico. La segunda etapa utiliza un proceso cromatográfico en lecho expandido desarrollado en un columna de absorción rellena con un soporte absorbente iónico denominado Streamline-DEAE. Por último, la tercera etapa es un proceso adicional cromatográfico en columna de intercambio iónico de tipo clásico que utiliza como fase estacionaria un lecho de DEAE-celulosa DE-52.Universidad de Almería. Universidad de Granada. Universidad de Jaé

An exploratory study of faculty perceptions regarding the management of university teaching

Resumen: En los últimos años han proliferado el número de investigaciones y publicaciones relacionadas con el nuevo papel que ha de desempeñar el profesor en el nuevo contexto universitario establecido por la convergencia con Europa, aunque consideramos que sigue siendo pertinente el desarrollo y presentación de trabajos relacionados con ciertos ámbitos de la docencia que inciden en el aumento de su calidad, como son la programación de asignaturas, los recursos didácticos utilizados y la consideración de la motivación del estudiante en el proceso de aprendizaje. En este trabajo se presentan las opiniones del profesorado sobre la importancia y uso que le conceden a estos tres ámbitos con respecto a su labor docente. Consideramos conveniente incidir en una mayor concienciación por parte de los profesores por mejorar su actividad docente, a través de grupos de trabajo interdisciplinares y de una formación didáctica centrada en el desarrollo curricular.Abstract: In recent years we have witnessed the proliferation in the number of research and publications related to the new role played by the teacher in the new university established by the convergence with Europe, although we believe it is still relevant the development and presentation of papers works on certain areas of teaching that influence the rise of its quality, such as scheduling of courses, teaching resources used and consideration of student motivation in the learning process. This paper presents the views of teachers on the importance and use to attach to these three áreas with regard to their teaching profession. We consider it desirable the increase of a greater awareness on the part of teachers to improve their teaching through interdisciplinary work groups and a training curriculum focusing on curriculum development

ESTUDIO EXPLORATORIO DE LAS PERCEPCIONES DEL PROFESORADO UNIVERSITARIO RESPECTO A LA GESTIÓN DE LA DOCENCIA

En los últimos años han proliferado el número de investigaciones y publicaciones relacionadas con el nuevo papel que ha de desempeñar el profesor en el nuevo contexto universitario establecido por la convergencia con Europa,aunque consideramos que sigue siendo pertinente el desarrollo y presentación de trabajos relacionados con ciertos ámbitos de la docencia que inciden en el aumento de su calidad, como son la programación de asignaturas, los recursos didácticos utilizados y la consideración de la motivación del estudiante en el proceso de aprendizaje. En este trabajo se presentan las opiniones del profesorado sobre la importancia y uso que le conceden a estos tres ámbitos con respecto a su labor docente.Consideramos conveniente incidir en una mayor concienciación por parte de los profesores por mejorar su actividad docente, a través de grupos de trabajo interdisciplinares y de una formación didáctica centrada en el desarrollo curricular.ABSTRACTIn recent years we have witnessed the proliferation in the number of research and publications related to the new role played by the teacher in the new  university established by the convergence with Europe, although we believe it is still relevant the development and presentation of papers works on certainareas of teaching that influence the rise of its quality, such as scheduling of courses, teaching resources used and consideration of student motivation in the learning process.This paper presents the views of teachers on the importance and use to attach to these three areas with regard to their teaching profession. We consider it desirable the increase of a greater awareness on the part of teachers to improve their teaching through interdisciplinary work groups and a training curriculum focusing on curriculum development

Assessing site quality using the National Forest Inventory

A pre-print version of the same manuscript is also available, which entitles "Assessing site quality using the National Forest Inventory"Sustainable production of wood is one of the main services provided by forest systems. Site productivity in the case of forests is often evaluated through the site quality. However, most of the works addressing the site quality have been done at local or regional scale. In this work, we aim to develop site quality models for five dominant species in Spanish forests (Fagus sylvatica, Pinus pinaster atlantica, Quercus pyrenaica, Pinus nigra, Pinus sylvestris) and create site quality maps at a national-scale from these models. First, we develop site quality models using site form (height-diameter relationship) as the reference index and the Spanish National Forest Inventory as dataset. Then, we fit spatial additive models entering physiographic and climatic variables in order to predict the site quality over the whole country. Additionally, we plot site form maps for the five species in order to describe spatial pattern in site quality at a national scale. Altitude and aspect appeared to be fundamental variables in the assessment of site quality. The accuracy of the spatial additive models ranged from 38.2% to 47.9%. The correspondence between the predicted and observed maps of site qualities is clear. Our results provide a tool which could be used by forest managers in land use planning as well as in forest policy decision-making at a national scale. We suggest that this method could be used in other countries and that the maps could be expanded to the European scale to assessing the way in which site quality varies across Europe always considering that the relationships between forest productivity and environmental variables could vary among biogeoclimatic zonesMarie Sklodowska-Curie Action, Towards a worldwide quantification of forest degradation (QUAFORD) 699884 AEI/FEDER, UE, AGL, AGL2016-76769-C2-2-R AEI/FEDER, UE, IJCI-2014-20614S

Polymeric Helical Structures à la Carte by Rational Design of Monomers

This document is the Accepted Manuscript version of a Published Work that appeared in final form in Macromolecules, copyright © 2020 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see: https://doi.org/10.1021/acs.macromol.0c00085Preparation of helical structures à la carte by monomer design of dynamic helical polymers such as poly(phenylacetylene)s (PPAs) is a difficult task due to conformational freedom of the polyene backbone. Herein, we study the monomer/helical polymer scaffold relationship by preparation of two novel phenylacetylene monomer series substituted at the phenyl ring in ortho-, meta-, or para-positions with the two enantiomers of either α-hydroxy-α-phenylacetic acid (1) or α-chloro-α-phenylacetic acid (S-2) linked through an anilide bond. These monomers were further polymerized, and their secondary structure and dynamic behavior were analyzed. Compiling information from these studies and the structural data for other PPAs found in the literature, we can state that anilide linkages in para-substituted polymers tend to generate compressed cis-cisoidal polyene structures, which can be transformed into more elongated cis-transoidal ones by external stimuli, while benzamide linkages in para-substituted polymers form mainly cis-transoidal scaffolds. The macromolecular structure of PPAs is also largely affected by the aromatic substitution pattern, adopting more stretched scaffolds once the pendant group is placed in meta- or ortho-positions, due to the steric hindrance generated by placing this group closer to the backboneFinancial support from MINECO (CTQ2015-70519-P), Xunta de Galicia (ED431C 2018/30; Centro singular de investigacioń de Galicia accreditation 2016-2019, ED431G/09, R.R. postdoctoral fellowship, and the European Regional Development Fund (ERDF) is gratefully acknowledgedS

Impact of atmospheric deposition on the metabolism of coastal microbial communities

11 páginas, 3 tablas, 5 figurasThe impact of rain water collected at marine, urban and rural sites on coastal phytoplankton biomass, primary production and community composition as well as the effect on microbial plankton metabolism was studied in 3 microcosm experiments conducted under contrasting spring, autumn and winter conditions. The measured responses were highly variable. Rainwater additions increased chlorophyll a (Chl a) concentration (5–68% difference between rainwater treatments relative to the control) in all experiments and reduced or stimulated primary production (PP) depending on the treatment and the experiment (from −10 to +169% relative to the control). Autotrophic stimulation was highest in spring, probably related to the low initial natural nutrient concentrations. Under winter nutrient replete conditions, rainwater inputs changed the phytoplankton community although this change did not promote increases in primary production. Enhancement of net autotrophy (increase of net oxygen production up to 227%) after rainwater inputs were only found during the period of low nutrient availability. Inputs of dissolved organic nitrogen (DON) explained a large fraction of the variability in the response of PP, Chl a, community respiration (CR) and net community production (NCP). Our results suggest that differences in the initial environmental conditions (i.e. nutrient availability), rainwater composition and the ability of the present autotrophic communities to utilize the new nutrients result in substantial changes in the microbial responses and associated biologically-mediated carbon fluxes. As atmospheric nutrient inputs into coastal oceans are increasing rapidly, our results help to understand the effects of different inputs on the metabolism of distinct microbial communitiesThis research was supported by the Galician Government (Xunta de Galicia) through the grants 07MMA002402PR (IMAN) and PGIDIT06PXIB312222PR (AddEx). S.M-G. and E.E.G-M. were funded by F.P.U. fellowships and E.T. by a Ramón y Cajal contract of the Spanish Ministry of Science and InnovationPeer reviewe