A Generic Undo Support for State-Based CRDTs

CRDTs (Conflict-free Replicated Data Types) have properties desirable for large-scale distributed systems with variable network latency or transient partitions. With CRDT, data are always available for local updates and data states converge when the replicas have incorporated the same updates. Undo is useful for correcting human mistakes and for restoring system-wide invariant violated due to long delays or network partitions. There is currently no generally applicable undo support for CRDTs. There are at least two reasons for this. First, there is currently no abstraction that we can practically use to capture the relations between undo and normal operations with respect to concurrency and causality. Second, using inverse operations as the existing partial solutions, the CRDT designer has to hard-code certain rules and design a new CRDT for almost every operation that needs undo support. In this paper, we present an approach to generic support of undo for CRDTs. The approach consists of two major parts. We first work out an abstraction that captures the semantics of concurrent undo and redo operations through equivalence classes. The abstraction is a natural extension of undo and redo in sequential applications and is straightforward to implement in practice. By using this abstraction, we then device a mechanism to augment existing CRDTs. The mechanism provides an "out of the box" support for undo without the involvement of the CRDT designers. We also present a practical application of the approach in collaborative editing

GIS Use in Oral Rabies Vaccine Programs

Frequent human and domestic animal exposures to rabid wildlife have raised the public\u27s awareness, leading to an increase in the number of wildlife submissions for rabies testing as well as an increase in the number of people requiring post exposure prophylaxis treatment. During 1998 and 1999, the Health and Human Services Department of a densely populated urban/suburban county in Virginia received a total of 955 animal submissions for rabies testing. Wildlife accounted for 714 of the submissions. Seventy-nine of the submitted wildlife were found dead, 445 were killed or euthanized for testing (190 unknown). Of the wildlife submissions,152 (21%) were positive, including 100 of 178 raccoons submitted. Human exposure, potentially requiring post-exposure prophylaxis, was recorded in 22 positive and 334 negative rabies submissions. Information was not available for 9 positive and 135 negative submissions; human exposure did not occur with the remaining submissions. To reduce the public\u27s risk of exposure to rabid animals, the County is developing a program to distribute oral rabies vaccine to raccoons. To increase the precision of vaccine delivery to raccoons, we propose the use of geographical information systems (GIS) as a method for selecting vaccination sites. Results from trapping and tracking studies, along with hydrographic and vegetation data, were utilized in the development of GIS generated vaccine distribution maps. Also factored in was human activity, commerce, residential housing density, competition by companion animals for vaccine bait, the location of refuse facilities, and property ownership. It is expected that this GIS supported approach will improve the efficiency of the program by lessening the cost while increasing the number of raccoons immunized. The resulting decrease in the incidence of rabies will lead to fewer human exposures to rabid wildlife, a decrease in the number of healthy wildlife euthanized for testing, and a decrease in the number of people requiring post-exposure prophylaxis treatment

MUTE: A Peer-to-Peer Web-based Real-time Collaborative Editor

International audienceReal-time collaborative editing allows multiple users to edit shared documents at the same time from different places. Existing real-time collaborative editors rely on a central authority that stores user data which is a perceived privacy threat. In this paper, we present MultiUser Text Editor (MUTE), a peer-to-peer web-based real-time collaborative editor without central authority disadvantages. Users share their data with the collaborators they trust without having to store their data on a central place. MUTE features high scalability and supports offline and ad-hoc collaboration

Two amino acid mutations in the capsid protein of type 2 porcine circovirus (PCV2) enhanced PCV2 replication in vitro and attenuated the virus in vivo

Porcine circovirus type 2 (PCV2) is the primary causative agent of postweaning multisystemic wasting syndrome (PMWS) in pigs. To identify potential genetic determinants for virulence and replication, we serially passaged a PCV2 isolate 120 times in PK-15 cells. The viruses harvested at virus passages 1 (VP1) and 120 (VP120) were biologically, genetically, and experimentally characterized. The PCV2 VP120 virus replicated in PK-15 cells to a titer similar to that of the PK-15 cell line-derived nonpathogenic PCV1 but replicated more efficiently than PCV2 VP1 with a difference of about 1 log unit in the titers. The complete genomic sequences of viruses at passages 0, 30, 60, 90, and 120 were determined. After 120 passages, only two nucleotide mutations were identified in the entire genome, and both were located in the capsid gene: the mutations were located at nucleotide positions 328 (C328G) and 573 (A573C). The C328G mutation, in which a proline at position 110 of the capsid protein changed to an alanine (P110A), occurred at passage 30 and remained in the subsequent passages. The second mutation, A573C, resulting in a change from an arginine to a serine at position 191 (R191S), appeared at passage 120. To experimentally characterize the VP120 virus, 31 specific-pathogen-free pigs were randomly divided into three groups. Ten pigs in group 1 received phosphate-buffered saline as negative controls. Each pig in group 2 (11 pigs) was inoculated intramuscularly and intranasally with 10(4.9) 50% tissue culture infective doses (TCID(50)) of PCV2 VP120. Each pig in group 3 (10 pigs) was similarly inoculated with 10(4.9) TCID(50) of PCV2 VP1. Viremia was detected in 9 of 10 pigs in the PCV2 VP1 group with a mean duration of 3 weeks, but in only 4 of 11 pigs in the PCV2 VP120 group with a mean duration of 1.6 weeks. The PCV2 genomic copy numbers in serum in the PCV2 VP1 group were significantly higher than those in the PCV2 VP120 group (P < 0.0001). Gross and histopathologic lesions in pigs inoculated with PCV2 VP1 were more severe than those inoculated with PCV2 VP120 at both day 21 and 42 necropsies (P = 0.0032 and P = 0.0274, respectively). Taken together, the results from this study indicated that the P110A and R191S mutations in the capsid of PCV2 enhanced the growth ability of PCV2 in vitro and attenuated the virus in vivo. This finding has important implications for PCV2 vaccine development

Does having a cat in your house increase your risk of catching COVID-19?

[EN]Due to the zoonotic origin of SARS-Coronavirus 2 (SARS-CoV-2), the potential for its transmission from humans back to animals and the possibility that it might establish ongoing infection pathways in other animal species has been discussed. Cats are highly susceptible to SARS-CoV-2 and were shown experimentally to transmit the virus to other cats. Infection of cats has been widely reported. Domestic cats in COVID-19-positive households could therefore be a part of a human to animal to human transmission pathway. Here, we report the results of a qualitative risk assessment focusing on the potential of cat to human transmission in such settings. The assessment was based on evidence available by October 2021. After the introduction of SARS-CoV-2 to a household by a human, cats may become infected and infected cats may pose an additional infection risk for other members of the household. In order to assess this additional risk qualitatively, expert opinion was elicited within the framework of a modified Delphi procedure. The conclusion was that the additional risk of infection of an additional person in a household associated with keeping a domestic cat is very low to negligible, depending on the intensity of cat-to-human interactions. The separation of cats from humans suffering from SARS-CoV-2 infection should contribute to preventing further transmission.SIThis work was funded by the German Federal Ministry of Education and Research within the COVMon Project, being part of the InfectControl2020 Initiative (BMBF grant no. 03COV16D)

Effect of Bovine Somatotropin on Neutrophil Functions and Clinical Symptoms During Streptococcus uberis Mastitis

The effect of recombinant bovine somatotropin (bST) on the chemiluminescence, diapedesis, and expression of adhesion receptors (CD11a, CD11b, CD18) of isolated polymorphonuclear leukocytes was studied. The plasma concentrations of insulin-like growth factor-I (IGF-I), bST, cortisol, and alpha-lactalbumin were also monitored. In addition, general and local clinical symptoms and the differentiation of circulating leukocytes were also studied during experimentally induced Streptococcus uberis mastitis in cows. Ten cows were infected with 500 cfu of S. uberis O140J in both left quarters. Five cows were subcutaneously treated with 500 mg of recombinant bST 7 d before and after infection, and 5 control cows received the excipient. General (fever, tachycardia, inappetance, and depression) and local symptoms (swelling, pain, firmness, and flecks in milk) were more acute, severe, and longer-lasting in control cows. Treatment with bST had no effect on chemiluminescence and diapedesis of circulating polymorphonuclear leukocytes and no effect on the expression of adhesion receptors. Recombinant bST induced significantly higher IGF-I and bST concentrations in plasma. The leukopenia observed after infection was less pronounced in the bST-treated cows, and the number of circulating band neutrophils and metamyelocytes was significantly lower in the treated group. The concentration of cortisol did not differ between both groups, but the blood concentration of alpha-lactalbumin significantly increased in both groups from 6 d after infection. These results showed that treatment with recombinant bST improves animal welfare by protecting the cows from severe local and general clinical symptoms during subsequent S. uberis mastitis, but that it has no effect on chemiluminescence, diapedesis, and the expression of adhesion receptors of circulating polymorphonuclear leukocytes