1,080 research outputs found
Inbreeding depression across the lifespan in a wild mammal population
Inbreeding depression is of major concern for the conservation of threatened species, and inbreeding avoidance is thought to be a key driver in the evolution of mating systems. However, the estimation of individual inbreeding coefficients in natural populations has been challenging, and, consequently, the full effect of inbreeding on fitness remains unclear. Genomic inbreeding coefficients may resolve the long-standing paucity of data on inbreeding depression in adult traits and total fitness. Here we investigate inbreeding depression in a range of life history traits and fitness in a wild population of red deer (Cervus elaphus) in Scotland using individual inbreeding coefficients derived from dense Single-Nucleotide Polymorphism (SNP) data (Fgrm). We find associations between Fgrm and annual breeding success in both sexes, and between maternal inbreeding coefficient and offspring survival. We also confirm previous findings of inbreeding depression in birth weight and juvenile survival. In contrast, inbreeding coefficients calculated from a deep and comparatively complete pedigree detected inbreeding depression in juvenile survival, but not in any adult fitness component. The total effect of inbreeding on lifetime breeding success (LBS) was substantial in both sexes: for Fgrm =0.125, a value resulting from a half-sib mating, LBS declined by 72% for females and 95% for males. Our results demonstrate that SNP-based estimates of inbreeding provide a powerful tool for evaluating inbreeding depression in natural populations, and suggest that, to date, the prevalence of inbreeding depression in adult traits may have been underestimated
Production of VEGF165 by Ewing's sarcoma cells induces vasculogenesis and the incorporation of CD34+ stem cells into the expanding tumor vasculature
The Ewing's sarcoma cell line TC71 overexpresses vascular endothelial growth factor isoform 165 (VEGF165), a potent proangiogenic molecule that induces endothelial cell proliferation, migration, and chemotaxis. CD34+ bone marrow stem cells can differentiate into endothelial and hematopoietic cells. We used a transplant model to determine whether CD34 + cells migrate from the bone marrow to Ewing's sarcoma tumors and participate in the neovascularization process that supports tumor growth. We also examined the role of VEGF165 in CD34+ cell migration. Human umbilical cord CD34+ cells were transplanted into sublethally irradiated severe combined immunodeficient mice. Seven days later, the mice were injected subcutaneously with TC71 tumor cells. Tumors were excised 2 weeks later and analyzed by immunohistochemistry. The tumor sections expressed both human VE-cadherin and mouse CD31, indicating involvement of donor-derived human cells in the tumor vessels. To determine the role of VEGF165 in the chemoattraction of CD34+ cells, we generated two VEGF 165-deficient TC71 clones, a stable anti-sense VEGF165 cell line (Clone 17) and a VEGF165 siRNA-inhibited clone (TC/siVEGF7-1). The resulting VEGF165-deficient tumor cells had normal growth rates in vitro, but had delayed growth when implanted into mice. Immunohistochemical analysis revealed decreased infiltration of CD34+ cells into both VEGF165-deficient tumors. These data show that bone marrow stem cells contribute to the growing tumor vasculature in Ewing's sarcoma and that VEGF165 is critical for the migration of CD34+ cells from the bone marrow into the tumor. © 2006 Wiley-Liss, Inc.Fil: Lee, Tim H.. University of Texas; Estados UnidosFil: Bolontrade, Marcela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. University of Texas; Estados UnidosFil: Worth, Laura L.. University of Texas; Estados UnidosFil: Guan, Hui. University of Texas; Estados UnidosFil: Ellis, Lee M.. University of Texas; Estados UnidosFil: Kleinerman, Eugenie S.. University of Texas; Estados Unido
Beam-induced Fe nanopillars as tunable domain-wall pinning sites
Focused-electron-beam-induced deposition (FEBID) is employed to create freestanding magnetic nanostructures. By growing Fe nanopillars on top of a perpendicular magnetic domain wall (DW) conduit, pinning of the DWs is observed due to the stray fields emanating from the nanopillar. Furthermore, a different DW pinning behavior is observed between the up and down magnetic states of the pillar, allowing to deduce the switching fields of the pillar in a novel way. The implications of these results are two-fold: not only can 3-dimensional nano-objects be used to control DW motion in applications, it is also proposed that DW motion is a unique tool to probe the magnetic properties of nano-objects
Maverick dark matter at colliders
Assuming that dark matter is a weakly interacting massive particle (WIMP)
species X produced in the early Universe as a cold thermal relic, we study the
collider signal of pp or ppbar -> XXbar + jets and its distinguishability from
standard-model background processes associated with jets and missing energy. We
assume that the WIMP is the sole particle related to dark matter within reach
of the LHC--a "maverick" particle--and that it couples to quarks through a
higher dimensional contact interaction. We simulate the WIMP final-state signal
XXbar + jet and dominant standard-model (SM) background processes and find that
the dark-matter production process results in higher energies for the colored
final state partons than do the standard-model background processes, resulting
in more QCD radiation and a higher jet multiplicity. As a consequence, the
detectable signature of maverick dark matter is an excess over standard-model
expectations of events consisting of large missing transverse energy, together
with large leading jet transverse momentum and scalar sum of the transverse
momenta of the jets. Existing Tevatron data and forthcoming LHC data can
constrain (or discover!) maverick dark matter.Comment: 11 pages, 7 figure
Voluntary exercise modulates pathways associated with amelioration of retinal degenerative diseases
Background: Exercise has been shown to promote a healthier and longer life and linked to a reduced risk of developing neurodegenerative diseases including retinal degenerations. However, the molecular pathways underpinning exercise-induced cellular protection are not well understood. In this work we aim to profile the molecular changes underlying exercise-induced retinal protection and investigate how exercise-induced inflammatory pathway modulation may slow the progression of retinal degenerations. Methods: Female C57Bl/6J mice at 6 weeks old were given free access to open voluntary running wheels for a period of 28 days and then subjected to 5 days of photo-oxidative damage (PD)-induced retinal degeneration. Following, retinal function (electroretinography; ERG), morphology (optical coherence tomography; OCT) and measures of cell death (TUNEL) and inflammation (IBA1) were analysed and compared to sedentary controls. To decipher global gene expression changes as a result of voluntary exercise, RNA sequencing and pathway and modular gene co-expression analyses were performed on retinal lysates of exercised and sedentary mice that were subjected to PD, as well as healthy dim-reared controls. Results: Following 5 days of PD, exercised mice had significantly preserved retinal function, integrity and reduced levels of retinal cell death and inflammation, compared to sedentary controls. In response to voluntary exercise, inflammatory and extracellular matrix integrity pathways were significantly modulated, with the gene expression profile of exercised mice more closely trending towards that of a healthy dim-reared retina. Conclusion: We suggest that voluntary exercise may mediate retinal protection by influencing key pathways involved in regulating retinal health and shifting the transcriptomic profile to a healthy phenotype
Single Top Production as a Window to Physics Beyond the Standard Model
Production of single top quarks at a high energy hadron collider is studied
as a means to identify physics beyond the standard model related to the
electroweak symmetry breaking. The sensitivity of the -channel mode,
the -channel -gluon fusion mode, and the \tw mode to various possible
forms of new physics is assessed, and it is found that the three modes are
sensitive to different forms of new physics, indicating that they provide
complimentary information about the properties of the top quark. Polarization
observables are also considered, and found to provide potentially useful
information about the structure of the interactions of top.Comment: References added and minor discussion improvements; results
unchanged; Version to be published in PR
The t W- Mode of Single Top Production
The t W- mode of single top production is proposed as an important means to
study the weak interactions of the top quark. While the rate of this mode is
most likely too small to be observed at Run II of the Fermilab Tevatron, it is
expected to be considerably larger at the CERN LHC. In this article the
inclusive t W- rate is computed, including O(1 / log (m_t^2 / m_b^2))
corrections, and when combined with detailed Monte Carlo simulations including
the top and W decay products, indicate that the t W- single top process may be
extracted from the considerable t tbar and W+ W- j backgrounds at low
luminosity runs of the LHC.Comment: 16 pages, 4 figure
Unusual raptor nests around the world
From surveys in many countries, we report using unusual nesting materials (e.g., paper money, rags, metal, antlers, and large bones) and unusual nesting situations. For example, we documented nests of Steppe Eagles [Aquila nipalensis] and Upland Buzzards [Buteo hemilasius] on the ground beside well-traveled roads, Saker Falcon [Falco cherrug] eyries in attics and a cistern, and Osprey [Pandian haliaetus] nests on the masts of boats and on a suspended automobile. Other records include a Golden Eagle [A. chrysaelos] nest 7.0 m in height, believed to be the tallest nest ever described, and, for the same species, we report nesting in rudimentary, nests. Some nest sites are within a Few meters of known predators or competitors. These unusual observations may be important in revealing the plasticity of a species' behavioral repertoire
An investigation into the prevalence of sleep disturbances in primary Sjögren’s syndrome: a systematic review of the literature
Objectives. To identify whether sleep disturbances are more prevalent in primary SS (pSS) patients compared with the general population and to recognize which specific sleep symptoms are particularly problematic in this population. Methods. Electronic searches of the literature were conducted in PubMed, Medline (Ovid), Embase (Ovid), PsychINFO (Ovid) and Web of Science and the search strategy registered a priori. Titles and abstracts were reviewed by two authors independently against a set of prespecified inclusion/exclusion criteria, reference lists were examined and a narrative synthesis of the included articles was conducted. Results. Eight whole-text papers containing nine separate studies met the inclusion criteria and were included in the narrative analysis. Few of these studies met all of the quality assessment criteria. The studies used a range of self-reported measures and objective measures, including polysomnography. Mixed evidence was obtained for some of the individual sleep outcomes, but overall compared with controls, pSS patients reported greater subjective sleep disturbances and daytime somnolence and demonstrated more night awakenings and pre-existing obstructive sleep apnoea. Conclusions. A range of sleep disturbances are commonly reported in pSS patients. Further polysomnography studies are recommended to confirm the increased prevalence of night awakenings and obstructive sleep apnoea in this patient group. pSS patients with excessive daytime somnolence should be screened for co-morbid sleep disorders and treated appropriately. Interventions targeted at sleep difficulties in pSS, such as cognitive behavioural therapy for insomnia and nocturnal humidification devices, have the potential to improve quality of life in this patient group and warrant further investigation
Recommended from our members
The low-resolution version of HadGEM3 GC3.1: development and evaluation for global climate
A new climate model, HadGEM3 N96ORCA1, is presented that is part of the GC3.1 configuration of HadGEM3. N96ORCA1 has a horizontal resolution of ~135 km in the atmosphere and 1° in the ocean and requires an order of magnitude less computing power than its medium-resolution counterpart, N216ORCA025, while retaining a high degree of performance traceability. Scientific performance is compared both to observations and the N216ORCA025 model. N96ORCA1 reproduces observed climate mean and variability almost as well as N216ORCA025. Patterns of biases are similar across the two models. In the north-west Atlantic, N96ORCA1 shows a cold surface bias of up to 6K, typical of ocean models of this resolution. The strength of the Atlantic meridional overturning circulation (16 to 17 Sv) matches observations. In the Southern Ocean, a warm surface bias (up to 2K) is smaller than in N216ORCA025 and linked to improved ocean circulation. Model El Niño/Southern Oscillation and Atlantic Multidecadal Variability are close to observations. Both the cold bias in the Northern hemisphere (N96ORCA1) and the warm bias in the Southern hemisphere (N216ORCA025) develop in the first few decades of the simulations. As in many comparable climate models, simulated interhemispheric gradients of top-of-atmosphere radiation are larger than observations suggest, with contributions from both hemispheres. HadGEM3 GC3.1 N96ORCA1 constitutes the physical core of the UK Earth System Model (UKESM1) and will be used extensively in the Coupled Model Intercomparison Project 6 (CMIP6), both as part of UKESM1 and as a stand-alone coupled climate model
- …