KONSEP REVITALISASI CITRA TEPIAN AIR DI RUANG TERBUKA KORIDOR SULTAN MUHAMMAD PONTIANAK

Revitalisasi merupakan salah satu upaya yang dikembangkan untuk menanggulangi kawasan yang mengalami degradasi. Kawasan yang sangat rentan mengalami degradasi/penurunan kualitas lingkungannya yaitu kawasan yang berada di tepian sungai. Upaya Pemerintah Kota Pontianak untuk menata kawasan tepian Sungai Kapuas sebagai ruang terbuka, untuk memunculkan citra Kota Pontianak sebagai kota tepian air, dan meningkatkan kembali vitalitas kawasan di pusat kota dengan tata guna lahan pusat perdagangan dan jasa. Berlokasi di Koridor Sultan Muhammad. Menarik perhatian penulis untuk memberikan masukan/ide berupa konsep ruang terbuka yang dapat menampilkan image kota tepian air. Metodologi yang dilakukan adalah mengumpulkan data dengan teknik observasi dan studi dokumentasi; analisis data berdasarkan potensi dan kendala yang ada pada kawasan. Hasil analisis adalah konsep perancangan untuk meningkatakan citra kawasan tepian air. Hasil konsep berdasarkan analisis terdiri dari : 1).Konsep Kegiatan, 2). Konsep Bentuk, 3). Konsep Bangunan, 4).Konsep Sirkulasi , 5).Konsep Perkerasan, 6).Konsep Penanda Kawasan,7). Konsep Utilitas Kawasan 8).Konsep Parkir, 9).Konsep Zoning dan 10).Tema Kawasan

Gamma Ray Spectroscopy with Scintillation Light in Liquid Xenon

Scintillation light from gamma ray irradiation in liquid xenon is detected by two Hamamatsu R9288 photomultiplier tubes (PMTs) immersed in the liquid. UV light reflector material, PTFE, is used to optimize the light collection efficiency. The detector gives a high light yield of 6 photoelectron per keV (pe/keV), which allows efficient detection of the 122 keV gamma-ray line from Co-57, with a measured energy resolution of (8.8+/-0.6)% (sigma). The best achievable energy resolution, by removing the instrumental fluctuations, from liquid xenon scintillation light is estimated to be around 6-8% (sigma) for gamma-ray with energy between 662 keV and 122 keV

Penentuan Konsep Pengembangan Kawasan Pedesaan di Kecamatan Tulung, Kabupaten Klaten

Menurut RTRW Kecamatan Tulung menjadi kawasan pertanian dan kawasan industri. Permukiman yang tumbuh di Kecamatan Tulung cenderung memusat di berbagai titik yang terdapat pusat aktivitas seperti industri. Permukiman di Kecamatan Tulung berkembang secara organik atau unplanned. Letak Kecamatan Tulung cukup strategis yaitu berada diantara Kabupaten Klaten dan Kabupaten Boyolali serta akses jalan kolektor Jatinom-Boyolali yang menghubungkan Kabupaten Boyolali dan Kabupaten Klaten juga membuat Kecamatan Tulung mudah dijangkau. Hal ini menyebabkan Kecamatan Tulung menjadi kawasan hunian yang ideal yang berdampak pada bertambahnya luas lahan permukiman tanpa ada control dari pemerintah. Konsep Eco Industrial Village diharapkan mampu menjawab masalah yang ada di Kecamatan Tulung. Metode pengumpulan data diperoleh melalui survei primer dan sekunder. Survei primer dilakukan dengan terjun langsung ke lapangan untuk melakukan observasi lapangan, wawancara, serta pemetaan denga Teknik analisis deskriptif. Tujuan penelitian ini dibagi dalam beberapa sasaran: pertama, penentuan konsep pengembangan wilayah. Kedua, melakukan pemetaan zonasi kawasan sesuai dengan konsep

Characterization of endogenous Kv1.3 channel isoforms in T cells

Producción CientíficaVoltage-dependent potassium channel Kv1.3 plays a key role on T-cell activation; however, lack of reliable antibodies has prevented its accurate detection under endogenous circumstances. To overcome this limitation, we created a Jurkat T-cell line with endogenous Kv1.3 channel tagged, to determine the expression, location, and changes upon activation of the native Kv1.3 channels. CRISPR-Cas9 technique was used to insert a Flag-Myc peptide at the C terminus of the KCNA3 gene. Basal or activated channel expression was studied using western blot analysis and imaging techniques. We identified two isoforms of Kv1.3 other than the canonical channel (54 KDa) differing on their N terminus: a longer isoform (70 KDa) and a truncated isoform (43 KDa). All three isoforms were upregulated after T-cell activation. We focused on the functional characterization of the truncated isoform (short form, SF), because it has not been previously described and could be present in the available Kv1.3−/− mice models. Overexpression of SF in HEK cells elicited small amplitude Kv1.3-like currents, which, contrary to canonical Kv1.3, did not induce HEK proliferation. To explore the role of endogenous SF isoform in a native system, we generated both a knockout Jurkat clone and a clone expressing only the SF isoform. Although the canonical isoform (long form) localizes mainly at the plasma membrane, SF remains intracellular, accumulating perinuclearly. Accordingly, SF Jurkat cells did not show Kv1.3 currents and exhibited depolarized resting membrane potential (VM), decreased Ca2+ influx, and a reduction in the [Ca2+]i increase upon stimulation. Functional characterization of these Kv1.3 channel isoforms showed their differential contribution to signaling pathways involved in formation of the immunological synapse. We conclude that alternative translation initiation generates at least three endogenous Kv1.3 channel isoforms in T cells that exhibit different functional roles. For some of these functions, Kv1.3 proteins do not need to form functional plasma membrane channels.Ministerio de Economía y Competitividad (grant PID 2020‐118517RB‐I00)Junta de Castilla y León (grants VA172P20) and (CLU-2019-02)Funds from Institut Curie, INSERM,Agence Nationale de la Recherche RetroTact (ANR‐20CE15‐0009‐01,ANR‐10‐IDEX‐0001‐02 PSL*, and ANR‐11‐LABX‐0043)Fondation pour la Recherche Médicale FRM (EQU202003010280

Myocardin-Dependent Kv1.5 Channel Expression Prevents Phenotypic Modulation of Human Vessels in Organ Culture

Objective: We have previously described that changes in the expression of Kv channels associate to phenotypic modulation (PM), so that Kv1.3 /Kv1.5 ratio is a landmark of vascular smooth muscle cells (VSMCs) phenotype. Moreover, we demonstrated that the Kv1.3 functional expression is relevant for PM in several types of vascular lesions. Here, we explore the efficacy of Kv1.3 inhibition for the prevention of remodeling in human vessels, and the mechanisms linking the switch in Kv1.3 /Kv1.5 ratio to PM. Approach and Results: Vascular remodeling was explored using organ culture and primary cultures of VSMCs obtained from human vessels. We studied the effects of Kv1.3 inhibition on serum-induced remodeling, as well as the impact of viral vector-mediated overexpression of Kv channels or myocardin knock-down. Kv1.3 blockade prevented remodeling by inhibiting proliferation, migration and extracellular matrix (ECM) secretion. PM activated Kv1.3 via downregulation of Kv1.5. Hence, both Kv1.3 blockers and Kv1.5 overexpression inhibited remodeling in a non-additive fashion. Finally, myocardin knock-down induced vessel remodeling and Kv1.5 downregulation and myocardin overexpression increased Kv1.5, while Kv1.5 overexpression inhibited PM without changing myocardin expression. Conclusions: We demonstrate that Kv1.5 channel gene is a myocardin-regulated, VSMCs contractile marker. Kv1.5 downregulation upon PM leaves Kv1.3 as the dominant Kv1 channel expressed in dedifferentiated cells. We demonstrated that the inhibition of Kv1.3 channel function with selective blockers or by preventing Kv1.5 downregulation can represent an effective, novel strategy for the prevention of intimal hyperplasia and restenosis of the human vessels used for coronary angioplasty procedures.This work was supported by grant BFU2016-75360-R from the Ministerio de Economía y Competitividad (MINECO), to MTPG and JRLL and Junta de Castilla y León Grant VA114P17 to MTPG. MAM and JS are predoctoral fellows of the UVa-Santander. KS was supported by the Swedish Research Council (2017-01225_3

Brazilian network for the surveillance of maternal potentially life threatening morbidity and maternal near-miss and a multidimensional evaluation of their long term consequences

<p>Abstract</p> <p>Background</p> <p>It has been suggested that the study of women who survive life-threatening complications related to pregnancy (maternal near-miss cases) may represent a practical alternative to surveillance of maternal morbidity/mortality since the number of cases is higher and the woman herself is able to provide information on the difficulties she faced and the long-term repercussions of the event. These repercussions, which may include sexual dysfunction, postpartum depression and posttraumatic stress disorder, may persist for prolonged periods of time, affecting women's quality of life and resulting in adverse effects to them and their babies.</p> <p>Objective</p> <p>The aims of the present study are to create a nationwide network of scientific cooperation to carry out surveillance and estimate the frequency of maternal near-miss cases, to perform a multicenter investigation into the quality of care for women with severe complications of pregnancy, and to carry out a multidimensional evaluation of these women up to six months.</p> <p>Methods/Design</p> <p>This project has two components: a multicenter, cross-sectional study to be implemented in 27 referral obstetric units in different geographical regions of Brazil, and a concurrent cohort study of multidimensional analysis. Over 12 months, investigators will perform prospective surveillance to identify all maternal complications. The population of the cross-sectional component will consist of all women surviving potentially life-threatening conditions (severe maternal complications) or life-threatening conditions (the maternal near miss criteria) and maternal deaths according to the new WHO definition and criteria. Data analysis will be performed in case subgroups according to the moment of occurrence and determining cause. Frequencies of near-miss and other severe maternal morbidity and the association between organ dysfunction and maternal death will be estimated. A proportion of cases identified in the cross-sectional study will comprise the cohort of women for the multidimensional analysis. Various aspects of the lives of women surviving severe maternal complications will be evaluated 3 and 6 months after the event and compared to a group of women who suffered no severe complications in pregnancy. Previously validated questionnaires will be used in the interviews to assess reproductive function, posttraumatic stress, functional capacity, quality of life, sexual function, postpartum depression and infant development.</p