Transcriptome pathways unique to dehydration tolerant relatives of modern wheat

Among abiotic stressors, drought is a major factor responsible for dramatic yield loss in agriculture. In order to reveal differences in global expression profiles of drought tolerant and sensitive wild emmer wheat genotypes, a previously deployed shock-like dehydration process was utilized to compare transcriptomes at two time points in root and leaf tissues using the Affymetrix GeneChip(R) Wheat Genome Array hybridization. The comparison of transcriptomes reveal several unique genes or expression patterns such as differential usage of IP(3)-dependent signal transduction pathways, ethylene- and abscisic acid (ABA)-dependent signaling, and preferential or faster induction of ABA-dependent transcription factors by the tolerant genotype that distinguish contrasting genotypes indicative of distinctive stress response pathways. The data also show that wild emmer wheat is capable of engaging known drought stress responsive mechanisms. The global comparison of transcriptomes in the absence of and after dehydration underlined the gene networks especially in root tissues that may have been lost in the selection processes generating modern bread wheats

3D excretory MR urography: Improved image quality with intravenous saline and diuretic administration

Purpose To assess the effect of diuretic administration on the image quality of excretory magnetic resonance urography (MRU) obtained following intravenous hydration, and to determine whether intravenous hydration alone is sufficient to produce diagnostic quality studies of nondilated upper tracts. Materials and Methods A total of 22 patients with nondilated upper tracts were evaluated with contrast-enhanced MRU. All patients received 250 mL of saline intravenously immediately prior to the examination. A total of 11 patients received 10–20 mg furosemide in addition to saline. Imaging was performed with a three-dimensional (3D) and two-dimensional (2D) breathhold spoiled gradient-echo sequences. Excretory MRU images were acquired five minutes after the administration of 0.1 mmol/kg gadolinium and were independently reviewed by two radiologists, who were blinded to the MRU technique. Readers evaluated the calyces, renal pelvis, and ureters qualitatively for degree of opacification, distention, and artifacts on a four-point scale. Statistical analysis was performed using a permutation test. Results There was no significant disagreement between the two readers ( P = 0.14). Furosemide resulted in significant improvement in calyceal and renal pelvis distention ( P < 0.005), and significant artifact reduction in all upper tract segments ( P < 0.001) compared to the effect of saline alone. Conclusion Intravenous furosemide significantly improves the image quality of excretory MRU studies obtained following intravenous hydration. Intravenous saline alone is insufficient to produce diagnostic quality studies of the non-dilated upper tracts. J. Magn. Reson. Imaging 2007. © 2007 Wiley-Liss, Inc.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/56023/1/20875_ftp.pd

A hierarchical detection method in external communication for self-driving vehicles based on TDMA

Security is considered a major challenge for self-driving and semi self-driving vehicles. These vehicles depend heavily on communications to predict and sense their external environment used in their motion. They use a type of ad hoc network termed Vehicular ad hoc networks (VANETs). Unfortunately, VANETs are potentially exposed to many attacks on network and application level. This paper, proposes a new intrusion detection system to protect the communication system of self-driving cars; utilising a combination of hierarchical models based on clusters and log parameters. This security system is designed to detect Sybil and Wormhole attacks in highway usage scenarios. It is based on clusters, utilising Time Division Multiple Access (TDMA) to overcome some of the obstacles of VANETs such as high density, high mobility and bandwidth limitations in exchanging messages. This makes the security system more efficient, accurate and capable of real time detection and quick in identification of malicious behaviour in VANETs. In this scheme, each vehicle log calculates and stores different parameter values after receiving the cooperative awareness messages from nearby vehicles. The vehicles exchange their log data and determine the difference between the parameters, which is utilised to detect Sybil attacks and Wormhole attacks. In order to realize efficient and effective intrusion detection system, we use the well-known network simulator (ns-2) to verify the performance of the security system. Simulation results indicate that the security system can achieve high detection rates and effectively detect anomalies with low rate of false alarms

On the feasibility of a channel-dependent scheduling for the SC-FDMA in 3GPP-LTE (mobile environment) based on a prioritized-bifacet Hungarian method

We propose a methodology based on the prioritization and opportunistic reuse of the optimization algorithm known as Hungarian method for the feasible implementation of a channel-dependent scheduler in the long-term evolution uplink (single carrier frequency division multiple access system). This proposal aims to offer a solution to the third generation system’s constraint of allocating only adjacent subcarriers, by providing an optimal resource allotment under a fairness scheme. A multiuser mobile environment following the third generation partnership project TS 45.005v9.3.0/25.943v9.0.0 was also implemented for evaluating the scheduler’s performance. From the results, it was possible to examine the channel frequency response for all users (four user equipments) along the whole bandwidth, to visualize the dynamic resource allocation for each of the 10,000 channel realizations considered, to generate the statistical distribution and cumulative distribution functions of the obtained global costs, as well as to evaluate the system’s performance once the proposed algorithm was embedded. Comparing and emphasizing the benefits of utilizing the proposed dynamic allotment instead of the classic static-scheduling and other existent methods.Peer ReviewedPostprint (published version

Molecular and functional heterogeneity of IL-10-producing CD4 + T cells

IL-10 is a prototypical&nbsp;anti-inflammatory cytokine, which is fundamental to the maintenance of immune homeostasis, especially in the intestine. There is an assumption that cells producing IL-10 have an immunoregulatory function. However, here we report that IL-10-producing CD4 + T cells are phenotypically and functionally heterogeneous. By combining single cell transcriptome and functional analyses, we identified a subpopulation of IL-10-producing Foxp3 neg CD4 + T cells that displays regulatory activity unlike other IL-10-producing CD4 + T cells, which are unexpectedly pro-inflammatory. The combinatorial expression of co-inhibitory receptors is sufficient to discriminate IL-10-producing CD4 + T cells with regulatory function from others and to identify them across different tissues and disease models in mice and humans. These regulatory IL-10-producing Foxp3 neg CD4 + T cells have a unique transcriptional program, which goes beyond the regulation of IL-10 expression. Finally, we found that patients with Inflammatory Bowel Disease demonstrate a deficiency in this specific regulatory T-cell subpopulation

The use of smart technologies in enabling construction components reuse: A viable method or a problem creating solution?

© 2017 The Authors. The exploitation of Radio Frequency Identification (RFID) for tracking and archiving the properties of structural construction components could be a potentially innovative disruption for the construction sector. This is because RFID can stimulate the reuse of construction components and reduce their wastage, hence addressing sustainability issues in the construction sector. To test the plausibility of that idea, this study explores the potential pre-conditions for RFID to facilitate construction components reuse, and develops a guidance for promoting their redistribution back to the supply chain. It also looks at how integrating RFID with Building Information Modelling (BIM) can possibly be a valuable extension of its capabilities, providing the opportunity for tracked components to be incorporated into new structures in an informed, sound way. A preliminary assessment of the strengths, weaknesses, opportunities and threats of the RFID technology is presented in order to depict its current and future potential in promoting construction components’ sustainable lifecycle management, while emphasis has been laid on capturing their technical, environmental, economic and social value. Findings suggest that the collection of the right amount of information at the design-construction-deconstruction-reuse-disposal stage is crucial for RFID to become a successful innovation in the construction sector. Although a number of limitations related to the technical operability and recycling of RFID tags seem to currently hinder its uptake for structural components’ lifecycle management, future technological innovations could provide solutions that would enable it to become a mainstream practice. Taken together these proposals advocate that the use of RFID and its integration with BIM can create the right environment for the development of new business models focused on sustainable resource management. These models may then unlock multiple values that are otherwise dissipated in the system. If the rapid technological development of RFID capability can be allied to policy interventions that control and manage its uptake along the supply chain, the sustainable lifecycle management of construction components could be radically enhanced.UK Engineering and Physical Sciences Research Council (EPSRC) and the UK Economic and Social Research Council (ESRC) who funded this work (Grant No. EP/K012398/1) in the context of the Infrastructure Business models, valuation and Innovation for Local Delivery (iBUILD) project