Mise Au Point Des Techniques De Micropropagation Pour Une Multiplication Massive De Gladiolus Segetum Et Gladiolus Dubius

Apical buds of corms of gladiolus (Gladiolus segetum and Gladiolus dubius) were cultivated on Murashige and Skoog (1962) medium supplemented with IBA 0.1 mg.l-1 and BA 2 mg.l-1. The number of newly formed buds was 3,8 and 4,13 buds per explants for respectively Gladiolus segetum and Gladiolus dubius. The in vitro Rooting need a supplement with auxin and sucrose. Rooting is not present in the control medium. The rate of rooting varied from 10 to 36%, depending on the species and the concentration of IBA in the medium. The Important rooting (36%) is observed for Gladiolus segetum in the presence of IBA 1 mg.l -1. Finally, the acclimatization is possible, these shoots rooted have formed many corms after 6 weeks of culture

A human ALDH1A2 gene variant is associated with increased newborn kidney size and serum retinoic acid

Nephron number varies widely between 0.3 and 1.3 million per kidney in humans. During fetal life, the rate of nephrogenesis is influenced by local retinoic acid (RA) level such that even moderate maternal vitamin A deficiency limits the final nephron number in rodents. Inactivation of genes in the RA pathway causes renal agenesis in mice; however, the impact of retinoids on human kidney development is unknown. To resolve this, we tested for associations between variants of genes involved in RA metabolism (ALDH1A2, CYP26A1, and CYP26B1) and kidney size among normal newborns. Homozygosity for a common (1 in 5) variant, rs7169289(G), within an Sp1 transcription factor motif of the ALDH1A2 gene, showed a significant 22% increase in newborn kidney volume when adjusted for body surface area. Infants bearing this allele had higher umbilical cord blood RA levels compared to those with homozygous wild-type ALDH1A2 rs7169289(A) alleles. Furthermore, the effect of the rs7169289(G) variant was evident in subgroups with or without a previously reported hypomorphic RET 1476(A) proto-oncogene allele that is critical in determining final nephron number. As maternal vitamin A deficiency is widespread in developing countries and may compromise availability of retinol for fetal RA synthesis, our study suggests that the ALDH1A2 rs7169289(G) variant might be protective for such individuals

Plantes vasculaires nouvelles ou rares pour la Tunisie presentes sur les iles (Galite, Zembra, Kuriat, Monastir, Kerkennah, Kneiss, Djerba)

Vascular plants new or rare for Tunisia occurring on the islands (Galite, Zembra, Kuriat, Monastir, Kerkennah, Kneiss, Djerba). - This study concerns the indigenous vascular plants newly reported for Tunisia and discovered on the islands during some field trips performed since 2014. Five native taxa are new to Tunisia: Bellis perennis, Carthamus creticus, Moraea mediterranea, Asplenium balearicum and Galium minutulum, the latter two also being new to North Africa. Information on 24 rare or poorly known taxa, most often of great biogeographical interest, is given, too. Most of these were discovered on the islands of the eastern coast (Kuriat, Monastir, Kerkennah, Kneiss, Djerba), but also on the northern islands of Zembra and La Galite. The new chorological data underline the first-rate conservation importance of these islands and islets for the preservation of the overall coastal biodiversity of Tunisia

Antibacterial activity of Thymoquinone, an active principle of Nigella sativa and its potency to prevent bacterial biofilm formation

<p>Abstract</p> <p>Background</p> <p>Thymoquinone is an active principle of <it>Nigella sativa </it>seed known as "Habbah Al-Sauda" in Arabic countries and "Sinouj" in Tunisia. Bacterial biofilms tend to exhibit significant tolerance to antimicrobials drugs during infections.</p> <p>Methods</p> <p>The antibacterial activity of Thymoquinone (TQ) and its biofilm inhibition potencies were investigated on 11 human pathogenic bacteria. The growth and development of the biofilm were assessed using the crystal violet (CV) and the 2, 3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) reduction assay.</p> <p>Results</p> <p>TQ exhibited a significant bactericidal activity against the majority of the tested bacteria (MICs values ranged from 8 to 32 μg/ml) especially Gram positive cocci (<it>Staphylococcus aureus </it>ATCC 25923 and <it>Staphylococcus epidermidis </it>CIP 106510). Crystal violet assay demonstrated that the minimum biofilm inhibition concentration (BIC50) was reached with 22 and 60 μg/ml for <it>Staphylococcus aureus </it>ATCC 25923 and <it>Staphylococcus epidermidis </it>CIP 106510 respectively. In addition our data revealed that cells oxidative activity was influenced by TQ supplementation. In the same way, TQ prevented cell adhesion to glass slides surface.</p> <p>Conclusion</p> <p>The ability of TQ to prevent biofilm formation warrants further investigation to explore its use as bioactive substances with antibiofilm potential.</p

MOLLUSCICIDAL AND LARVICIDAL ACTIVITIES OF Atriplex inflata AERIAL PARTS AGAINST THE MOLLUSK Galba truncatula, INTERMEDIATE HOST OF Fasciola hepatica

Fasciolosis is a widespread parasitosis of farm live-stock in many developing countries. For this reason, it is necessary to search for new substances against parasitic diseases caused by flukes. Indeed, a wide variety of terrestrial plants have been subjected to chemical and pharmacological screening in order to discover their potential for human medicinal use. The molluscicidal and larvicidal activities of Atriplex inflata were tested on Galba truncatula and Fasciola hepatica larval stages infecting this snail in Tunisia. Phytochemical tests were conducted on extracts in order to establish a meaningful relationship with molluscicidal and larvicidal activities. The molluscicidal activity was evaluated by subjecting snails to sample aqueous solutions. Accordingly, hexane, ethyl acetate, methanol and methanol-water (8:2, v-v) were used as extraction solvents. As a result, hexane and ethyl acetate extracts showed potent activity, according to the World Health Organization, giving LC50 = 7.59 mg/L and 6.69 mg/L for hexane extracts of leaves and fruits, respectively. Ethyl acetate extracts gave LC50 = 5.90 mg/L and 7.32 mg/L for leaves and fruits, successively. Molluscicidal activities of powders were less potent on snails, but active according to the World Health Organization. Hexane and ethyl acetate extracts from leaves and fruits gave potent larvicidal activities with a delay rate exceeding 45.50% (45.50- 98.92%). Phytochemical tests showed that these activities may be attributed to the presence of triterpenoids and/or sterols

Plantes vasculaires nouvelles ou rares pour la Tunisie présentes sur les îles (Galite, Zembra, Kuriat, Monastir, Kerkennah, Kneiss, Djerba)

International audienceVascular plants new or rare for Tunisia occurring on the islands (Galite, Zembra, Kuriat, Monastir, Kerkennah, Kneiss, Djerba).-This study concerns the indigenous vascular plants newly reported for Tunisia and discovered on the islands during some field trips performed since 2014. Five native taxa are new to Tunisia: Bellis perennis, Carthamus creticus, Moraea mediterranea, Asplenium balearicum and Galium minutulum, the latter two also being new to North Africa. Information on 24 rare or poorly known taxa, most often of great biogeographical interest, is given, too. Most of these were discovered on the islands of the eastern coast (Kuriat, Monastir, Kerkennah, Kneiss, Djerba), but also on the northern islands of Zembra and La Galite. The new chorological data underline the first-rate conservation importance of these islands and islets for the preservation of the overall coastal biodiversity of Tunisia